Chemical Profiles and Bioactivities of Ethanol and Water Extracts of Sclerotium and Fruiting Body of Lignosus rhinocerotis (Cooke) from Banggai Islands, Indonesia
http://www.doi.org/10.26538/tjnpr/v6i12.13
Keywords:
xanthine oxidase inhibition, UPLC-QTOFMS/MS, Polyporaceae, Lignosus rhinocerotis, α-glucosidase inhibitionAbstract
Lignosus rhinocerotis (tiger milk mushroom), a sclerotium-forming mushroom, has been shown
to have various pharmacological properties. However, its chemical constituents were not much
investigated. In addition, its enzymes’ inhibitory potentials are not yet described. Hence, the
study aimed to investigate the chemical compositions of its sclerotium and fruiting body. The
UPLC-QTOF-MS/MS was used to analyze ethanol and water extracts of both sclerotium and
fruiting bodies. The ethanol extracts of the sclerotium and fruiting body were investigated for
their antioxidant (DPPH and phosphomolybdenum) and enzymes (α-glucosidase, xanthine
oxidase, and trypsin) inhibitory activities. The UPLC-QTOF-MS/MS analyses showed the
presence of adenosine, chelidimerine, and pingpeimine B in the water extracts of the sclerotium
and the fruiting body. Whereas cytidine, feroxin A, hirsuteine, mangiferin, neomangiferin and
sophoraisoflavone A were identified in ethanol extracts of the sclerotium and the fruiting body.
The fruiting body showed higher phenolic content than the sclerotium. The fruiting body also
exhibited relatively higher radical scavenging and reducing activities, as well as α-glucosidase
inhibition activities, when compared with the sclerotium. However, both the sclerotium and the
fruiting body showed slight xanthine oxidase (compared to allopurinol) and trypsin inhibition
activities. Findings provide a basis for further exploration of phytotherapeutic applications of L.
rhinocerotis.
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