Plectranthus scutellarioides Leaf Extract Protective Effects Against Isoniazid and Rifampicin-Induced Hepatotoxicity in Wistar Rats

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Lusi P. Dwita
Ni Putu E. Hikmawanti
Juhairoh Husniah
Muhammad Hazraj
Siti N. Bela
Eva Aryanti


Plectranthus scutellarioides has been studied to have antioxidant and anti-inflammatory activity which shows potential with hepatoprotection effect. This study determined the hepatoprotective activity of ethanol extract of P. scutellarioides leaves. The rats were divided into five groups: PSE (Plectranthus scutellarioisdes leaves extract) 150, 300, and 600 mg/kg, negative control, and positive control (Silymarin). All groups were given the test substances concurrently with isoniazid and rifampicin (50 mg/kg) p.o every day for 28 days. The results showed that the PSE could prevent liver damage as indicated by lower levels of total bilirubin, direct bilirubin,albumin, AST, and ALT than negative controls (p<0.005). Histological observations of the liver confirmed these results, and this dose-dependent hepatoprotective activity was in line with the lipid peroxidation inhibition. P. scutellarioides extract at a dose of 600 mg/kg showed the best activity, comparable (p> 0.05) to Silymarin. The data suggest that the co-administration of P. scutellarioides leaf extract with isoniazid and rifampicin could prevent the liver injury caused by these drugs.

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Dwita, L. P., Hikmawanti, N. P. E., Husniah, J., Hazraj, M., Bela, S. N., & Aryanti, E. (2022). Plectranthus scutellarioides Leaf Extract Protective Effects Against Isoniazid and Rifampicin-Induced Hepatotoxicity in Wistar Rats: Tropical Journal of Natural Product Research (TJNPR), 6(12), 1936–1940. Retrieved from


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