The Protective Effect of Sa-khan (Piper, Piperaceae) Against High Glucose-Induced Cytotoxicity in Human Proximal Tubular Epithelial Cells http://www.doi.org/10.26538/tjnpr/v6i12.9

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Published: Dec 30, 2022
Keywords:
Wound scratch assay, MTT, HK-2, High glucose-induced cytotoxicity, Sa-khan

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Daoyot Daorueang
School of Allied Health Sciences, University of Phayao, Phayao, 56000, Thailand

Abstract

High glucose-induced cytotoxicity plays a critical role in the pathogenesis of diabetic kidney disease (DKD). The present study aimed to investigate the potential nephroprotective effect of Sa-khan (Piper, Piperaceae) against high glucose-induced cytotoxicity in a human proximal tubular epithelial cell line. The cytotoxicity induced by high glucose in HK-2 cells, a human proximal tubular epithelial cell line, was studied using the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay. The wound scratch assay was performed to evaluate the migration capability of HK-2 cells. Treating cells with high glucose significantly reduced the viability of HK-2 cells (p < 0.001). Sa-khan crude extract (SCE) at concentration of 0.031 mg/mL was found to increase the viability of high glucose-induced HK-2 cells (p = 0.013). In addition, high glucose significantly increased the migration capability of HK-2 cells (p < 0.001). Furthermore, SCE at concentrations of 0.016 and 0.031 mg/mL abolished the increased migration capability of high glucose-induced cells (p < 0.001). This study shows that Sa-khan crude extract displays a potential protective effect against high glucose-induced proximal tubular cell injury.

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How to Cite
Daorueang, D. (2022). The Protective Effect of Sa-khan (Piper, Piperaceae) Against High Glucose-Induced Cytotoxicity in Human Proximal Tubular Epithelial Cells: http://www.doi.org/10.26538/tjnpr/v6i12.9. Tropical Journal of Natural Product Research (TJNPR), 6(12), 1953-1956. https://tjnpr.org/index.php/home/article/view/1382
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Vol. 6 No. 12 (2022): Tropical Journal of Natural Product Research (TJNPR)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biography

Daoyot Daorueang, School of Allied Health Sciences, University of Phayao, Phayao, 56000, Thailand

 

 

 

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