Erratum Bioethanol Production from Pineapple and Cassava Peels Using Fungal Isolates as Inoculants http://www.doi.org/10.26538/tjnpr/v6i9.27
Main Article Content
Abstract
Globally, the production of biofuels as an alternative energy source from renewable raw materials to complement energy needs has become of utmost importance. The goal of this study was to compare bioethanol production from pineapple and white cassava peels utilizing inoculants such as Neurospora crassa, Aspergillus oryzae and Saccharomyces cerevisiae. The cassava and pineapple peels were obtained from the white cassava and abacaxi pineapple, respectively. The fungal isolates: Neurospora crassa, Aspergillus oryzae, and Saccharomyces cerevisiae were isolated from burnt wood, steamed rice, and fresh palm wine (from oil palm) at 37ºC. Sabouraud Dextrose Agar (SDA) at pH 5.6 was used to grow the fungal isolates, and morphological observations were used to confirm the isolates. The substrates were fermented with different inoculant combinations, distilled on days 4 and 8, and the physicochemical parameters were determined. The results showed that Aspergillus oryzae and Saccharomyces cerevisiae in combination gave the highest bioethanol yield of 48.67±5.7 ml with the pineapple peels at day 8; whereas Aspergillus oryzae and Neurospora crassa in combination gave the highest bioethanol yield of 38.33±2.03 ml with the cassava peels at day 4. This observation was statistically significant (p<0.05). The findings led to the conclusion that pineapple peels have a higher bioethanol yield than cassava peels. The inoculants utilized in this research work indicate the best prospects for bioethanol production from abacaxi pineapple and white cassava.
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References
Dermirbas A. Progress and recent trends in biofuels. Progress in Energy and Combustion Science. 2007; 33(1):1-18.
Khambalker VP, Katkhede SS, Dahatonde S, Korfe ND, Nage SM. Renewable energy: an assessment of public awareness. Intl J of Ambient Energy. 2011; 31:133-142.
Mckinney ML, Schoch RM. Environmental Science, Systems and Solutions. Third edition, Jones and Bartlett Publishers, USA. 2003; 28p.
Millew GT. Living the environment: Principles, connections and solutions. Fourth edition, Brooks /Cole Publishing Company, Pacific Grove, USA. 1999; 178p.
Gupta R and Dermirbas A. Review of Gasoline, diesel, and Ethanol Biofuels from Grasses and plants. New York: Cambridge University Press. 2011; 202p.
Baird C, Cann M, Freemand WH. Biofuels and other Alternative fuels. Environmental Chemistry Fifth Edition. W. H. Freeman & Co Publishers, USA. 2012; 291p.
Hahn-Hagerdal B, Galbe M, Gorwa-Graishind MF, Liden G, Lacchi G. Bio-ethanol – the fuel of tomorrow from the residues of today. Trends Biotechnol. 2006; 24(12):549-556.
Alternative Fuels Data Center. Alternative Fuels comparison chart. 2003.
Shapouri H, James D, Michael W. The energy balance of corn ethanol: an update. United States Department of Agriculture, Office of the Chief Economist, Office of Energy Policy and New Uses. Agricultural Economic Report No. 813. 2002; 20p.
Ferreira S, Durate AP, Ribeiro MH, Queine JA, Domingues FC. Response surface optimization of enzymatic hydrolysis of cistus ladanifer and cytisusstriatus for bioethanol production. Biochem Engineering J. 2009; 43:192-200.
Taherzadeh MJ and Karimi K. Enzyme-based hydrolysis processes for ethanol from lignocellulosic materials; a review: Bioresources. 2007; 2(4):707-738.
Talebrua F, Karakashew D, Angelidaki I. Production of bioethanol from wheat straw: an overview on pretreatment, hydrolysis and fermentatim. Bioresource Tech. 2010; 101:7402- 7409.
Deesuth O, Laopaiboon P, Jaisil P, Laopaiboon L. Optimization of Nitrogen and metal ions supplementation for very high gravity bioethanol fermentation from sweet sorghum juice using an orthogonal array design. Energies, MDPL. 2012; 9:1- 20.
Ingram LO, Gomez PF, Lai X, Moniruzzaman M, Wood BE, Yomano LP, York SW. Metabolic Engineering of bacteria for ethanol production. Biotechnol Bioeng. 1998; 58(2-3):204-214.
Feldman H. Yeast. Molecular and Cell Biology. Wiley- Blackwell, USA. 2010; 464p.
Cogliati M. Global molecular epidemiology of Cryptococcus neoformans and Cryptococcus yaltan: An atlas of the molecular types. Scientifica (Cairo). 2013; 2013:675213.
Davis RH and Perkins DD. Neurospora: a model of model microbes. Nat Rev Genet. 2002; 3:392-403.
Jacobson DJ. Neurospora in temperate forest of Western North America. Mycologia. 2004; 96:66-74.
Rao M, Deshpande V, Keskar S, Srinivasan MC. Cellulase and ethanol production from cellulase by Neurospora crassa. Enzyme and Microb Technol. 1983; 5:133–136.
Shurleff W and Aoyagi A. History of koji – grains and/or soybeans enrobed with a mold culture (300 BCE to 2021): extensively annotated bibliography and source book. Soyinfo Center, USA. 2021; 812p.
Tailor MJ, Richardson T. Applications of microbial enzymes in food systems and in biotechnology. Adv Appl Microbiol. 1979; 25:7-35.
Machida M, Asai K, Sano M, Tanaka T, Terai G, Kusumolo KJ, Arima T, Akita O, Kumaza J, Kashiwagi, Y. Genome sequencing and analysis of Aspergillus oryzae. Nature. 2005; 438:1157-1161.
Dermirbas A. The importance of bioethanol and biodiesel from biomass. Energy Sources Econ Plan Policy. 2008; 3(2):177- 185.
Olabisi OO. Isolation and characterization of palm wine strains of Saccharomyces cererisiae potentially useful as baking yeast. Euro J Exp Biol. 2017; 7:11-16.
Machida M. Progress of Aspergillus enzyme Genomics. Research Centre for Glycoscience. National Institute of Advanced Industrial Science and Technology (AIST) Central. 2002; 6:1-7.
Hsiao-Che KSHJC, Fredrick O, Asiegbu J, Valkonen PT, Yong-Hwan L. Secret lifestyle of Neurospora crassa. Sci Rep. 2014; 30(4):5135.
Oyeleke SB, Jibrin NM. Production of bioethanol from guinea corn husk and millet husk. Afr J Microbiol Res. 2009; 3(4):147- 152.
Snehal I, Sanket J, Akshaya G. Production of bioethanol using agricultural waste: banana pseudo stem. Braz J Microbiol. 2014; 45(3):885-892.
Essien JP, Akpan EJ, Essien EP. Studies on mould growth and biomass production using waste banana peels. World J Microbiol Biotechnol. 2003; 8:13-29.
Mohammed J, Akanksha G, Durlubh K. Production of bioethanol from fruit wastes (banana, papaya and mango peels) under milder conditions. J Bioprocess Biotechnol. 2018; 8(3):327.
Shilpa C, Malhotra G, Chanchal. Alcohol production from fruit and vegetable waste. Intl J Appl Eng. 2013; 16(2):199-206.
Zainal A, Ellena S, Tadjudin N. Bioethanol production from waste of cassava peel by acid hydrolysis and fermentation process. Intl J Pharm Technol Res. 2014; 6(4):1209-1212.
Chibuzor O, Edak AU, Igile G. Bioethanol production from cassava peels using different microbial inoculants. Afr J Biotechnol. 2016; 15(30):1608-1612.
Wikipedia Contributors. Gravity (alcoholic beverage). In Wikipedia, The Free Encyclopedia. 5 March 2019, retrieved 28 July 2020, from https://en.wikipedia.org/w/index.php?title=Gravity_(alco holic_beverage)&oldid=886343596
Engineering Toolbox. Density, Specific Weight and Specific Gravity. [online] Available at: https://www.engineeringtoolbox.com/density-specific-weight- gravity-d_290.html. 2003; [Accessed 28th July, 2020.].
NCDOI. Chemical and Physical Characteristics of Ethanol and Hydrocarbon Fuels.
https://ncdoi.com/OSFM/RPD/PT/Documents/Coursework/Eth anol/Module2_InstructorGuide.pdf ) Assessed 28th July, 2020.
Brian J. The importance of specific gravity. Dynamix agitator Inc British Coloumbia V6V 1L4. 2015.
Lim Y, Jang Y, Kim K. Production of a high concentration of potato tuber by high gravity fermentation. Food Sci Biotechnol. 2013; 22(2):441-448.
Ly H, Block D, Longo M. Interfacial tension effect of ethanol on lipid bilayer rigidity, stability, and are/molecule: a micropipette approach. Bioscience. 2002; 18:88-95.
Walker G. Magnesium as a stress-protectant for industrial strains of Saccharomyces cerevisiae. J Am Soc Brew Chem. 1998; 56:109-113.
Yuhong H, Jin Y, Zhao Y. Viscosity reduction during fuel ethanol production by fresh sweet potato fermentation. Chin J Appl Environ Biol. 2012; 4(4):661-666.