Isolation and Evaluation of Pharmacological Activities of a Bioactive Hydroxylated C28 Steroid from the Leaf of Laportea decumana (Roxb.) Wedd doi.org/10.26538/tjnpr/v6i7.9
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Abstract
Cancer and infection rates have risen significantly in the last decade. Medicinal plants can be used to treat cancer and infections. Laportea decumana (Roxb.) Wedd for example, has long been used as a medicinal plant, but scientific evidence on its pharmacological effects is limited. The present study was therefore aimed at isolating a bioactive compound from the leaves of Laportea decumana and evaluating its pharmacological activities. Ethanol was used to extractthe leaves of L. decumana using the maceation method. Preparative thin layer chromatography (TLC) was used for the isolation of an active compound, and preparative high-performance
liquid chromatography (HPLC) was employed for purification. The structure of the active compound was determined by UV/Vis, IR, MS, 1H-NMR, 13C-NMR, HMQC, and HMBC. Staphylococcus aureus and Escherichia coli were used to examine the antibacterial activity of
the compound. Furthermore, the antioxidant potential of the new compound was determined by the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. Molecular docking was performed with the PyRx program. Also, the compound was tested for its ability to inhibit 4T1 cancer cells. The results showed that the extraction process yielded an oily green, thick extract with a yield value of 5.19%. From the leaves of L. decumana, a new hydroxylated C28 steroid was isolated (MCI). The bioactive compound possessed antibacterial properties against the test organisms, as well as antioxidant activity by DPPH assay. Furthermore, MCI was active against 4T1 cancer cells. The
findings of this study suggest that MCI has the potential as a cancer cell inhibitor.
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