Evaluation of Antioxidant and Antimicrobial Activities of Stachys Leaves Extract http://www.doi.org/10.26538/tjnpr/v6i9.22
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Abstract
The genus Stachys L. (Lamiaceae) includes about 300 species located in temperate and tropical regions across the world Mediterranean, Asia, America and southern Africa. Several species of this genus are extensively used in various traditional medicines of special pharmacological interest are considered the anti-inflammatory, antioxidant, analgesic, anxiolytic and antidepressant activity. The aim of this study was to evaluate the bioactive compounds, antioxidants and antimicrobial activity of Stachys leaves extract. The antioxidant activity was determined by measuring total phenolic content (TPC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). Methanol extracts of Stachys leaves extract and evaluated for its antimicrobial activity. The antibacterial activity was assessed using paper disc method against two bacteria namely Staphylococcus aureus and E. coli. Showed that leaves extracts presented the highest phenolic
compounds content for both species. Furthermore, GC-MS analysis allowed the identification of 37 bioactive compounds regardless of Stachys leaves extract. Leaves extract of Stachys possessed the highest total phenol content and antioxidant activity (DPPH). The antimicrobial tests showed that leaves extracts of Stachys exhibited the highest activity against two bacterial strains (Staphylococcus aureus and Escherichia coli). The studied leaves of Stachys is considered an excellent source of natural bioactive molecules that could be an interesting material for medicinal and food purposes.
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Klemow KM, Bartlow A, Crawford J, Kocher N, Shah J, Ritsick M. Herbal Medicine: Biomolecular and Clinical Aspects; CRC Press: Boca Raton, FL, USA, 2011; 211–228.
Kostic DA, Dimitrijevic DS, Stojanovic GS, Palic IR, Dordevic AS, Ickovski JD. Xanthine oxidase: Isolation, assays of activity, and inhibition. J. Chem. 2015: 8:294858.
Kabir MSH, Hossain MM, Kabir MI, Ahmad S, Chakrabarty N, Rahman MA, Rahman MM. Antioxidant, antidiarrheal, hypoglycemic and thrombolytic activities of organic and aqueous extracts of Hopea odorata leaves and in silico PASS prediction of its isolated compounds. BMC Complem Altern Med. 2016; 16:474.
Mabberley DJ. The plant book: a portable dictionary of the vascular plants. 2nd ed. Cambridge (UK): 1997. Cambridge University Press.
Piozzi F, Paternostro MP, Servettaz O, Arnold NA. Occurrence of (þ)-6-desoxyandalusol in Stachys ionica and Stachys distans. Biochem Syst E.col. 2002; 33:887-889.
Grujic-Jovanovic S, Skaltsa HD, Marin P, Sokovic M. Composition and antimicrobial activity of the essential oil of six Stachys species from Serbia. Flavor Fragr J. 2004. 19:139–144
Aydin A, Sener B, Cakici I, Turan NN, Erdemolgu N. Antioxidant activities of some Lamiaceae plant extracts. Phytother Res. 2006; 20:9-93.
Amirghofran Z, Bahmani M, Azadmehr A, Javidnia K. Anticancer effects of various Iranian native medicinal plants on human tumour cell lines. Neoplasma. 2006; 53:428-433.
Khanavi M, Sharifzadehb M, Hadjiakhoondi A, Shafieec A. Phytochemical investigation and anti-inflammatory activity of aerial parts of Stachys byzanthina C. Koch. J Ethnopharmacol. 2005; 97:463–468.
Hayashi K, Nagamatsu T, Ito M, Hattori T, Suzuki Y. Acetoside, a component of Stachys sieboldii MIQ, may be a promising antinephritic agent: effect of acteoside on crescentic-type anti-GBM nephritis in rats. Jpn J Pharmacol.1994; 65:143–151
Stamatis G, Kyriazopoulos P, Golegou S, Basayiannis A, Skaltsas S, Skaltsa H. In vitro anti-Helicobacter pylori activity of Greek herbal medicines. J Ethnopharmacol. 2003; 88:175–179.
Rabbani M, Sajjdi SE, Zarei HR. Anxiolytic effects of Stachys lavandulifolia Vahl on the elevated plus-maze model of anxiety in mice. J Ethnopharmacol. 2003; 89:271–276.
Conforti F, Menichini F, Formisano C, Rigano D, Senatore F, Arnold NA, Piozzi F. Comparative chemical composition, free radical-scavenging and cytotoxic properties of essential oils of six Stachys species from different regions of the Mediterranean Area. Food Chem. 2009; 116:898–905.
Edeoga HO, Okwu DE, Mbaebie, BO. Phytochemical constituents of some Nigerian medicinal plants. Afr. J. Biotechnol.2005; 4:685–688.
Musa KH, Abdullah A, Jusoh K, Subramaniam V. Antioxidant activity of pink-flesh guava (PsidiumguajavaL.): effect of extraction techniques and solvents, Food Analytical Methods., 2011; 4(1):100-107.
Kamali M, Khosroyar S, Mohammadi A. Antibacterial activity of various extracts from Dracocephalum kotschyi against food pathogenic microorganisms. Int.J. PharmTech Res. 2015; 8(9):158-163.
Skaltsa HD, Demetzos C, Lazari D, Sokovic M. Essential oil analysis and antimicrobial activity of eight Stachys species from Greece, Phytochemistry, 2003; 64(3):743-752,
Hussain AI, Anwar F, Hussain S, Syed T, Przybylski R. "Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations". Food Chemistry. 2008; 108(3):986–995.
Paul Erasto and Alvaro MV. Limonene - A Review: Biosynthetic, Ecological and Pharmacological Relevance. Natural Product Communications. 2008; 3(7):1193-1202.
Xue Liu, Jiaxin Cai, Haiming Chen, Qiuping Zhong, Yaqi Hou, Weijun Chen. Antibacterial activity and mechanism of linalool against Pseudomonas aeruginosa, Microbial Pathogenesis. 2020; 141:103980.
Brand-Williams W, Cuvelier ME, Berset C. Use of a freeradical method to evaluate antioxidant activity. Food Sci Technol. 1995; 28(1):25-30.
Rahman MM, Islam MB, Biswas M, Khurshid Alam AHM. In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Research Notes.2015; 8:621-630.
Gulçin I. Antioxidant activity of food constituents: an overview. Arch Toxicol. 2012; 86(3):345–391.
Kamali H, Ahmadzadeh Sani T, Mohammadi A, Alesheikh P, Khodaverdi E, Hadizadeh F. A comparison between pressurized hot water and pressurized liquid extraction for optimizing phenolic and antioxidants capacity of the wooden layer between of walnut seed. J SUPERCRIT FLUID. 2018; 133(1):535-541.
Namvar K, Mohammadi A, Ataei SE, Feyzi P. Evaluation of Solvent Effect (Methanol: Water Mixture) on the Phenolic Content and Antioxidant Activities of Stachys turcomanica Trautv. J Pharm Sci. 2017; 23:244-248.
Rosello-Soto E, Marti-Quijal FJ, Cilla A, Munekata PES, Lorenzo JM., Remize F, Barba FJ. Influence of Temperature, Solvent and pH on the Selective Extraction of Phenolic Compounds from Tiger Nuts By-Products: Triple-TOF-LCMS-MS Characterization. Molecules. 2019; 24(4):797-810.
Donkor AM, Gumah BK, Akugbire AE. Evaluation of Antibacterial Potentiation of Crude Extracts of Phyllanthus amarus, Tamarindus indica and Cleome viscosa and Their Formulation. Int J Plant Res. 2014; 4(1):23-28.