Study of the Histological Changes Caused by the Intake of Metformin, Carnitine and Omega-3 in Some Organs of Male Diabetic Rabbits
http://www.doi.org/10.26538/tjnpr/v6i9.21
Keywords:
T2DM, Omega-3, Metformin, L-carotene, Diabetes mellitus, AlloxanAbstract
Diabetes mellitus (DM) refers to a group of diseases that affect how the body uses blood sugar. The condition is often managed with medications. Metformin helps to manage blood sugar levels. Carnitine is an adjuvant treatment for type 2 diabetes, and omega-3 can be taken in conjunction with metformin to reduce diabetic complications. Many of the drugs for managing DM may affect vital organs in the body. Therefore, the present study was conducted to examine the effects of metformin, L-carnitine, and omega-3 fatty acids on the histological changes of the liver and kidney cells in diabetic rabbits. Forty-five (45) male rabbits weighing 1500-1800 g and aged 10-14 months were divided into 5 groups: the control group (1), the alloxan-induced DM group (2), the DM group that received metformin treatment (3), the DM group that received metformin and L-carnitine treatment (4), and the DM group that was administered with
metformin and omega-3 (5). The results of the study revealed that several histological changes associated with DM were observed. This was represented by calcification of the bile ducts of the liver, pigmentation of the nuclei, degeneration of some cells, and extensive necrosis. Also, the kidney showed glomerulus defect (necrosis & atrophy), and vacuoles some tubules. The findings of the present study revealed that the histology of the rabbits' livers and kidneys in the treated and untreated groups differed significantly. Therefore, this study recommends caution while using medications to manage diabetes mellitus.
References
Galicia-Garcia U, Asier B, Shifa J, Asier L, Haziq S, Kepa BU, Helena O, César M. Pathophysiology of type 2 diabetes mellitus. Int. J Mol Sci. 2020; 21:6275-6311.
Chih-Yuan W, David LN, Philip H. 2020 vision – An overview of prospects for diabetes management and prevention in the next decade. Diat Res Clin. 2018; 143:101–112.
Sami W, Tahir A, Nadeem SB, Mohd RH. Effect of diet on type 2 diabetes mellitus: A review. Int J Health Sci (Qassim). 2017; 11(2):65-71.
Milne N and Di Rosa F. The diabetes review: A guide to the basics. J Diab Nur. 2020; 24:161.
Dai X, Luo ZC, Zhai L, Zhao WP, Huang F. Adverse drug events associated with low-dose (10 mg) versus high-dose (25 mg) empagliflozin in patients treated for type 2 diabetes mellitus: A systematic review and meta-analysis of randomized controlled trials. Diabetes Ther. 2018; 9(2):753-770.
Ito M, Mitsumasa F, Yuka S, Hiroyuki W. Eiichi I. Carnitinerelated hypoglycemia caused by 3 days of pivalate antibiotic therapy in a patient with severe muscular dystrophy: a case report. BMC Pediatrics. 2017; 17:73.
Karabulut N. Effects of L-carnitine and magnesium on some biochemical parameters in experimental diadiabetes-inducedts. PhD Thesis, University of Erciyes, Turkey. 2017; 21-34p.
Senturk M, Meryem E, Zeynep SS. Investigation of the effects of L-carnitine and magnesium on oxidative stress and cytokines in the tissue of experimental diabetic rats. Acta VeterinariaBeograd. 2021; 71(4):477-489.
Novakova K, Kummer O, Bouitbir J, Stoffel SD, HoerlerKoerner U, Bodmer M, Roberts P, Urwyler A, Ehrsam R, Krähenbühl S. Effect of L-carnitine supplementation on the body carnitine pool, skeletal muscle energy metabolism and physical performance in male vegetarians. Eur J Nutr.2016; 55(1):207-17.
Lima Rocha JÉ, Mendes Furtado M, Mello Neto RS, da Silva Mendes AV, Brito AKdS, Sena de Almeida, JOC, Rodrigues Queiroz EI, de Sousa França JV, Silva Primo MG, Cunha Sales ALdC. Effects of fish oil supplementation on oxidative stress biomarkers and liver damage in hypercholesterolemic rats. Nutrients. 2022; 14:426.
Adeva-Andany MM, Calvo-Castro I, Fernández-Fernández C, Donapetry-García C, Pedre-Piñeiro AM. Significance of Lcarnitine for human health. IUBMB Life. 2017; 69(8):578-594.
Borum PR. Carnitine. Ann, Rev, Nutr. 1983; 3:233-259.
Wood AHR, Chappell HF, Zulyniak MA. Dietary and supplemental long-chain omega-3 fatty acids as moderators of cognitive impairment and Alzheimer's disease. Euro J Nut 2022; 61(2):589–604.
Anu A, Kylie AA, Mark M, Peter WS, Manohar LG. Longchain omega-3 polyunsaturated fatty acids and cognitive decline in non-demented adults: a systematic review and metaanalysis. Nutr Rev. 2020; 78(7):563–578.
Hashimoto M and Shahdat H. Fatty acids: From membrane ingredients to signaling molecules. Biochemistry and Health Benefits of Fatty Acids book. 2018; 1-5p.
Calder PC. Omega-3 fatty acids and inflammatory process: from molecules to man. United Kingdom. 2017; 1-21p.
Zatalia SR and Sanusi, H. The role of antioxidants in the pathophysiology, complications, and management of diabetes mellitus. Acta Med, Indones Arp. 2013; 45(2):141-7.
Misra M and Aiman, U. Alloxan. An unpredictable drug for diabetes induction? Ind J Pharm. 2012; 44(4):538-539.
Akhtar MS, Ahmed M, Gulzar K, Adnan H. Hypoglycemia activity of Dodonaea viscosa leaves in normal alloxan-induced diabetic rabbits. Dia Betologia. 2011; 40(3):71-79.
Presnell J K and Schreibman M P. Humason’s animal tissue techniques, 5th edition. John Hopkins Univ. Balfimore. 1997; 546 p.
Brzoska M, Moniuszko-Jakoniuk J, AL-Marcinkie-wicz B, Sawicki B. Liver and kidney function and Histology in rats exposed to cadmium and ethanol. Alcohol. 2003; 32:2-10.
Sagano M, Yamato H, Hayashi T, Ochia H, Kakucki J, Goto S, Nishijima F, Ino N, Kazama J, Takeuchi T, Mokuda O, Ishikawa T, and Okazaki R. High-fat diet in low-dose streptozotocin-treated heminphrectomized rats induces all features of human. J Nutr Metab Cardio. 2006; 16(7):477-84.
Ragavan B and Krishnakumari S. Effect of T. Arjuna stem Bark extract on histopathology of liver, kidney, and pancreas of alloxan-Induced Diabetic Rtats. Afr J Bio Med. 2006; 9:189- 197.
Ramaiah SK, Rachel M, Apte UM. Hepatocyte Proliferation is the Possible Mechanism for the Transient Decrease in Liver Injury during the steatosis stage of alcoholic liver disease. Toxic Pathol. 2004; 32(5):567-576.
Al-Zorri SG. Some physiological and Histological effects of alcoholic extract Tribulus terrestris in diabetic female rabbits. B.Sc. Bio. College of science. 2009; 15-28p.
Mir SH, Abdul-Baqui B, Darzi, MM, Abdul-Whid S. Biochemical and histomorphological study of streptozotocin– induced diabetes mellitus in rabbits. Pak J Nut. 2008; 7(2):359- 364.
Lin HZ, Xang SQ, Chucharee FK, Ronnrt G, Diehl AM. Metformin revises fatty liver disease in obsess leptin-deficient mice, J Nat Med. 2000; 998-1003.
Zheng H, Piao S, Sun L, Zhao H, Jin J, Jin J, Yang C, Li C. Renoprotective effects of L-carnitine in streptozotocininduced diabetic nephropathy. Int J Clin Exp Med. 2018; 1:4459-4469.
Curran, RC and Crocker J. Curran atlas of histopathology, 4th ed. Havery miller publisher oxford university press, USA. 2008; PP:172-204.
Gomaa AM and Abd-EL-Aziz E. Omega-3 fatty acids decrease oxidative stress, tumor necrosis factor-alpha, and interleukin-1 beta in hyperthyroidism induced hepatic dysfunction rat model. J Pathophys, 2016; 23:295-301.
EL-Moghazy M, Zesdan NS, EL-Atrsh AM, EL-Gogry M, Tousson E. The possible effect of diets containing fish oil (omega-3) on hematological, biochemical, and histopathogical alterations of rabbit liver and kidney. Biomed Pre Nutr. 2014; 4(3):371-377.
Bassey EO, Oyebadejo S, Ikanna A. Histopathological assessment of the kidney of alloxan induced diabetic rat treated with macerated Allium sativum (garlic). Asia J Bio med and Pharm Sci. 2014; 4(35):13-17.
Pourghasem M, Nasiri E, Shafi H. Early Renal Histological changes in Alloxan Induced Diabetic Rats. Int. J Mol Cell Med. 2014; 3(1):11-15.
Roy R, Kamat SG, Mascarenhas C. Prophylactic effect of dietary supplementation of fish oil extracted from sardinella longiceps on renal dysfunction in alloxan induced diabetic mice. J Diab Obes. 2016; 3(1):8-15.
Karch AM. Focus on nursing pharmacology, 4th ed. Walters Kluwer Lippincott Williams and Wilkins, 2008; 555,728,988 p.
Krishna V. Textbook of pathology. 1st ed. Orient Long man private. India. 2004; 538p.
Zhang S, Xu H, Yu X, Wu Y, Sui D. Metformin ameliorate diabetic nephropathy in a rat model of Low dose streptozotocin-induced diabetes. Exp Therap Med. 2017; 4(1259):383-390.
Howard T and Karsner MD. Human pathology, School of Medicine, Western, Reserve University, Cleveland. 1986; 42, 90, 665, 720p.
Barone J and Hills B. Usmle, Pathology, step 1. Kaplan med Inc. 2004; 50p.
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