In Vitro Aggregation Inhibition Activity of n-Hexane Leaf Extract of Azadirachta indica of Human Platelet

http://www.doi.org/10.26538/tjnpr/v6i9.25

Authors

  • Linus A. Nwaogu Department of Biochemistry, School of Biological Sciences, Federal University of Technology, P.M.B. 1526, Owerri, Imo State, Nigeria
  • Chidi U. Igwe Department of Biochemistry, School of Biological Sciences, Federal University of Technology, P.M.B. 1526, Owerri, Imo State, Nigeria
  • Adaku V. Iwueke University of Agriculture and Environmental Sciences, Umuagwo, Imo State, Nigeria
  • Chieme S. Chukwudoruo Department of Biochemistry, School of Biological Sciences, Federal University of Technology, P.M.B. 1526, Owerri, Imo State, Nigeria

Keywords:

Leaves, Neemstick, Inhibition, Aggregation, Phytochemistry, Antiplatelet activity

Abstract

In Nigeria, Azadirachta indica leaf is used traditionally in the treatment of various diseases. The present study aims at investigating the science behind in vitro aggregation inhibition activity of n-hexane leaf extract of Azadirachta indica of human platelet which can be used for the prevention and management of stroke, which has been found to be a common cause of morbidity in Nigeria. The study was carried out using platelet-rich plasma of fifty female student volunteers of blood group O+ with different concentrations of Azadirachta indica leaf extract obtained with n-hexane as extraction solvent. Three concentrations: 0.5, 2.0 and 4.0 mg/mL of
Azadirachta indica leaf extract were used. Aspirin (2.0mg/mL) and distilled water served as the positive and negative controls respectively. Preliminary phytochemical screening of Azadirachta indica leaves revealed the presence of saponins, flavonoids, tannins, steroids, alkaloids, cardiac glycosides, terpenoids and phenols. The Azadirachta indica leaf extract showed a dose effect on percentage platelet aggregation inhibition of 55% at 2 mg/mL higher when compared to aspirin which gave 40% at the same concentration. The results obtained revealed that Azadirachta indica leaf extract exhibited considerable percentage platelet aggregation inhibition and
antiplatelet activity higher than aspirin possibly due to some important phytochemical constituents of the leaf and thus can be used in the prevention and management of stroke. 

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Published

2022-09-01

How to Cite

Nwaogu, L. A., Igwe, C. U., Iwueke, A. V., & Chukwudoruo, C. S. (2022). In Vitro Aggregation Inhibition Activity of n-Hexane Leaf Extract of Azadirachta indica of Human Platelet: http://www.doi.org/10.26538/tjnpr/v6i9.25. Tropical Journal of Natural Product Research (TJNPR), 6(9), 1487–1491. Retrieved from https://tjnpr.org/index.php/home/article/view/1332