Vincamine Reduces Cell Viability of KB and Hep-2 Cancer Cells Through its Apoptotic Potential
http://www.doi.org/10.26538/tjnpr/v6i9.13
Keywords:
Apoptosis, Cell viability, Hep-2 cells, KB cells, VincamineAbstract
Analysing in vitro anti-proliferative or cytotoxic efficacy of medicinal plants or their bioactive principles would provide preliminary information to assess their anticancer efficacies under in vivo experimental animal models. The anti-proliferative efficacy of vincamine on the HeLa subline of the keratinizing tumour cell line (KB cells) and human epithelial type 2 cells (Hep-2 cells) was investigated in the present study. Nuclear DNA fragmentation, changes in mitochondrial membrane potential (MMP) and chromatin condensation as well as level of reactive oxygen species (ROS) generation was utilized as biomarkers to demonstrate the antiproliferative efficacy of vincamine. Western blotting protocol was employed to evaluate the expression pattern of apoptosis-related proteins such as mutant p53, Bcl-2, Bax, and caspase-3. Vincamine treatment to KB and Hep-2 cells caused reduction in cell proliferation of these cancer cell lines in a dose dependent fashion. Also, treatment with vincamine led to an enhancement in the generation of ROS via activation of MMP depolarization. A significant morphological alteration accompanied by apoptotic cell death was observed in the vincamine treated KB and Hep-2 cells. Bcl-2 and mutant p53 protein expression were markedly down regulated by vincamine, while Bax and caspase-3 status were markedly increased by vincamine in the KB and Hep-2 cells. These findings suggest that vincamine might have the ability to serve as a potent anti-proliferative agent through its apoptotic potential. The findings of this investigation thus explore the capability of vincamine to suppress the proliferation of KB and Hep-2 cells, possibly through its apoptosis inducing potential.
References
Subramanyam GK, Gaddam SA, Kotakadi VS, Palithya S, Penchalaneni J, Challagundla VN. Argyreia nervosa (Samudra pala) leaf extract mediated silver nanoparticles and evaluation of their antioxidant, antibacterial activity, in vitro anticancer and apoptotic studies in KB oral cancer cell lines. Artif Cells NanomedBiotechnol. 2021; 49(1):635-650.
Guo HF and Wang MH. Dichloromethane fraction of Asiasarumheterotropoides induces S phase arrest and apoptosis in KB oral epithelial carcinoma cells. Biomed Pharmacother. 2017; 89:559-564.
Önay Ö, Köse S, Süslü N, Korkusuz P, Nemutlu E, Aydın C, Hoşal Ş. Human laryngeal squamous cell carcinoma cell line release of endogenous anandamide and 2-arachidonoylglycerol, and their antiproliferative efficacy via exogenous supplementation: an in vitro study. Cell Tissue Bank. 2022; 23(1):93-100.
Krishnaveni MK, Suresh R, Arunkumar. Antiproliferative and Apoptotic Efficacys of Quinine in Human Hep-2 Laryngeal Cancer and KB Oral Cancer Cell. Bangladesh J Pharmacol. 2016; 11(3):593-02.
Fernald K andKurokawa M. Evading apoptosis in cancer. Trends Cell Biol. 2013; 23(12):620-33.
Aborehab NM, Elnagar MR, Waly NE. Gallic acid potentiates the apoptotic efficacy of paclitaxel and carboplatin via overexpression of Bax and P53 on the MCF-7 human breast cancer cell line. J Biochem Mol Toxicol. 2021; 35(2):e22638.
Ebrahimi M, Babaei E, Neri F, Feizi MAH. Anti-proliferative and apoptotic efficacy of gemini curcumin in p53-wild type and p53-mutant colorectal cancer cell lines. Int J Pharm. 2021; (15)601:120592.
Li Y, Zou Q, Zhang J. Vincamine exerts protective efficacy on spiral ganglion neurons in endolymphatic hydrops guinea pig models. Am J Transl Res. 2018; 10(11):3650-3663.
Fayed AH. Brain trace element concentration of rats treated with the plant alkaloid, vincamine. Biol Trace Elem Res. 2010; 136(3):314-9.
Nandini HS and Naik PR. Antidiabetic, antihyperlipidemic and antioxidant efficacy of Vincamine, in streptozotocin-induced diabetic rats. Eur J Pharmacol. 2019; 15; 843:233-239.
Fischhof PK, Möslinger-Gehmayr R, Herrmann WM, Friedmann A, Russmann DL. Therapeutic efficacy of vincamine in dementia. Neuropsychobiology. 1996; 34(1):29-35.
Erdo SA, Molnár P, Lakics V, Bence JZ, Tömösközi Z. Vincamine and vincanol are potent blockers of voltage-gated Na+ channels. Eur J Pharmacol. 1996; 24; 314(1-2):69-73.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 16; 65(1-2):55-63.
Rastogi RP, Singh SP, Häder DP, Sinha RP. Detection of reactive oxygen species (ROS) by the oxidant-sensing probe 2’,7’- dichlorodihydrofluorescein diacetate in thecyanobacterium Anabaena variabilis PCC 7937. Biochem Biophys Res Commun. 2010; 2; 397(3):603-7.
Scaduto RC Jr and Grotyohann LW. Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives. Biophys J. 1999; 76(1 Pt 1):469-77.
Baskić D, Popović S, Ristić P, Arsenijević NN. Analysis of cycloheximide-induced apoptosis in human leukocytes: fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide. Cell Biol Int. 2006; 30(11):924-32.
Böhmer RM and Ellwart J. Combination of BUdR-quenched Hoechst fluorescence with DNA-specific ethidium bromide fluorescence for cell cycle analysis with a two-parameter flow cytometer. Cell Tissue Kinet. 1981; 14(6):653-8.
Al-Rashed S, Baker A, Ahmad SS, Syed A, Bahkali AH, Elgorban AM, Khan MS. Vincamine, a safe natural alkaloid, represents a novel anticancer agent. Bioorg Chem. 2021; 107:104626.
Wu H, Chen L, Zhu F, Han X, Sun L, Chen K. The Cytotoxicity Efficacy of Resveratrol: Cell Cycle Arrest and Induced Apoptosis of Breast Cancer 4T1 Cells. Toxins (Basel). 2019; 13:11(12):731.
Pajaniradje S, Mohankumar K, Pamidimukkala R, Subramanian S, Rajagopalan R. Antiproliferative and apoptotic efficacys of Sesbania grandiflora leaves in human cancer cells. Biomed Res Int. 2014; 2014:474953.
Baracca A, Sgarbi G, Solaini G, Lenaz G. Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesis. BiochimBiophys Acta. 2003; 1606(1-3):137-46.
He B, Lu N, Zhou Z. Cellular and nuclear degradation during apoptosis. CurrOpin Cell Biol. 2009; 21(6):900-12.
Kochubei T, Kitam V, Maksymchuk O, Piven O, Lukash L. Possible mechanisms of Leukoagglutinin induced apoptosis in human cells in vitro. Cell Biol Int. 2016; 40(12):1313-1319.
Vinothkumar V, Manoharan S, Sindhu G, Nirmal MR, Vetrichelvi V. Geraniol modulates cell proliferation, apoptosis, inflammation, and angiogenesis during 7,12- dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis. Mol Cell Biochem. 2012; 369(1-2):17-25.
Manjari V, Murugesan S, Banumathi V, Pattarayan R.Anticancer Activity of PanchaPaasanaChendhuram on MCF-7 Cells. Int J NutrPharmacol Neurol Dis 2018; 8:143-52.
Ahmad S, Badr B, Khan A, Rehman R, Ghias K, Muhammad JS, Khan MR. The Role of K-Ras and P53 in Biliary Tract Carcinoma. J Pak Med Assoc. 2021; 71(10):2378-2384.
Gbemisola SM, Ayorinde JB, Sunday OA, Olubunmi MA, Olawale FO, Olufemi OO, Meyer M. Gene Expression Modulation of Apoptotic and Oestrogen Receptor Alpha Genes by Active Fractions of Selected Nigerian Plants on Cervical Cancer Cell Line (HeLa). Trop J Nat Prod Res. 2021; 5(4):772- 777.
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