Antimicrobial Activity and Chemical Composition Analysis of Jasminum sambac L. and Plumeria alba L. Flower Extracts
doi.org/10.26538/tjnpr/v6i3.6
Keywords:
Antimicrobial, Ethanol extract, Jasminum sambac L, Phytochemical, Plumeria alba L.Abstract
Jasminum sambac L. and Plumeria alba L. are known to have antimicrobial properties. Thisresearch was conducted to determine the antimicrobial activity and chemical composition of crude ethanol extracts from J. sambac and P. alba flowers. J. sambac and P. alba flowers were obtained, air-dried, and extracted with ethanol. The final J. sambac and P. alba flower ethanol extracts were prepared in ratios of 1:1, 1:2, and 2:1, with concentrations of 25 and 50 mg/mL, respectively. Antimicrobial activity was tested against Staphylococcus aureus, Escherichia coli and Candida albicans. Qualitative phytochemical and Gas Chromatography-Mass Spectrometry (GC-MS) analyses were conducted to determine the phytoconstituents of the flower extracts. The maximum inhibition zone of J. sambac and P. alba (1:2) ratio against S. aureus (8.68±0.15 mm) and coli (8.59±0.09 mm) was obtained at 50 and 25 mg/mL for 6 h, respectively. Furthermore, the highest C. albicans inhibition zone of 7.54±0.27 mm was achieved after 24 h using a 25 mg/mL J. sambac: P. alba (2:1) ratio. The phytochemical analysis showed that both flower extracts contained tannin, flavonoid, saponin, and phenol. After that, GC-MS analysis of sambac revealed 24 compounds, the majority of which were made up of 9,12,15-octadecatrienal, octadecanoic acid, and 9,12-octadecadienoic acid. There were 44 compounds with a higher percentage of cyclohexanol, octadecanoic acid, and squalene in the Plumeria alba GC-MS analysis. The findings of this study suggest that ethanol extracts of J. sambac and P. alba flowers could be employed as antibacterial agents.
References
Gu Y. Raising awareness of antimicrobial resistance: comment on reducing expectations for antibiotics in primary care: a randomized experiment to test the response to fear-based messages about antimicrobial resistance. BMC Med. 2020; 18(1):1-2.
Azam F, Rahman MS, Amin GM Al, Miah I, Koh Y. Delafloxacin, a superior antibacterial agent to its competitor fluor
oquinolones comparative activity of delafloxacin with other fluoroquinolones. Korean J Microbiol. 2021; 57(3):133-141
.
Bhatia P, Sharma A, George AJ, Anvitha D, Kumar P, Dwivedi VP, Chandra, NS. Antibacterial activity of medicinal p
lants against ESKAPE : an update. Heliyon. 2021; 7:e06310.
Chanthasena P, Penkhrue W, Khowangklang P, Sritangos P, Nantapong N. Antimicrobial potential of fungi isolated fro
m soils of a dry dipterocarp forest in northeast Thailand. Chiang Mai J Sci. 2021; 48(3):793-807.
Yau JW, Thor SM, Tsai D, Speare T, Rissel C. Antimicrobial stewardship in rural and remote primary health care: a nar
rative review. Antimicrob Resist Infect Contr. 2021; 10(1):1-33.
Su PW, Yang CH, Yang JF, Su PY, Chuang LY. Antibacterial activities and antibacterial mechanism of Polygonum cuspidatum extract against nosocomial drug-resistant pathogens. Molecules. 2015; 20(6):11119-11130.
Astuti P, Rollando R, Wahyuono S, Nurrochmad A. Antimicrobial activities of isoprene compounds produced by an en
dophytic fungus isolated from the leaves of Coleus amboinicus Lour. J Pharm Pharmacogn Res. 2020; 8(4):280-289.
Al-mamoori F, Al-tawalbe DM, Alnaqeeb M. Medicinal plants for the treatment and management of oral infections: a r
eview. Trop J Nat Prod Res. 2021; 5(9):1528-1536.
Ankomah AD, Boakye YD, Agana TA, Adu F, Agyare C. Antimicrobial properties of Cnestis ferruginea Vahl ex DC. Heliyon. 2021; 7:e08266.
Gupta A, Jeyakumar E, Lawrence R. Journey of limonene as an antimicrobial agent. J Pure Appl Microbiol. 2021; 15(3
):1094-1110.
Siva M, Shanmugam KR, Shanmugam B, Venkata SG, Ravi S, Sathyavelu RK, Mallikarjuna K. Ocimum sanctum: a re
view on the pharmacological properties. Int J Basic Clin Pharmacol. 2016; 5(3):558-565.
Egharevba, GO, Dosumu OO, Oguntoye SO, Njinga NS, Olatunde S, Hamid AA, Anand A, Amtul, Z, Priyanka, U. An
tidiabetic, antioxidant and antimicrobial activities of extracts of Tephrosia bracteolata leaves. Heliyon. 2019; 5:e02275.
Primiani CN, Pujiati P, Setiawan MA. Phytochemical constituents and antimicrobial activity of Elaeocarpus sphaericus
Schum seed extract. Trop J Nat Prod Res. 2021; 5(10):1775-1781.
Sabharwal S, Sudan S, Ranjan V. Jasminum sambac Linn (Motia): a review. Int J Pharm Res Bio-Science. 2013; 2(5):1 08-130.
Chen G, Mostafa S, Lu Z, Du R, Cui J, Wang Y, Liao, Q, Lu J, Mao Q, Chang B, Wang L, Jia Z, Yang X, Zhu Y, Yan J, Jin B. The jasmine (Jasminum sambac) genome and flower fragrances. bioRxiv. 2020. 1-25 p.
Kumar S, Vishwavidyalaya GK, Vishwavidyalaya GK, Gautam SS. Screening of antimicrobial properties of Jasminum
sambac Linn. leaf extracts against dental pathogens. Res J Phytochem. 2015; 9(4):195-200.
Idrees S, Hanif MA, Ayub MA, Jilani MI, Memon N. Frangipani. Medicinal Plants of South Asia. Elsevier Ltd. 2020; 287-300p.
Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal. 201
; 6(2):71-79.
Setyani W, Yakub J, Yandri O, Kawan VR. Phytochemical investigation and antibacterial activity ethanol extract of pa
paya seeds (Carica papaya L.) applicated for gel product. J Ilmu Kefarmasian Indones. 2020; 18(1):96-100.
Rohmah J, Saidi IA, Rini CS, Putri ZA, Tiana KH, Putri CTR. Antioxidant activity assay of white Turi (Sesbania grand
iflora (L.) Pers) extracts using DPPH radical scavenging method. Pharmaciana. 2020; 10(3):257-268.
Mensah-Agyei GO, Ayeni KI, Ezeamagu CO. GC-MS analysis of bioactive compounds and evaluation of antimicrobia
l activity of the extracts of Daedalea elegans: A Nigerian mushroom. Afr J Microbiol Res. 2020; 14(6):204-210.
Awang-Jamil Z, Aminuddin MF, Zaidi BQ, Basri AM, Ahmad N, Taha H. Phytochemicals and antimicrobial analysis
of selected medicinal plants from Brunei darussalam. Biodiversitas. 2021; 22(2):601-606.
Nascimento GGF, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antib
iotic-resistant bacteria. Braz J Microbiol. 2000; 31(4):247-256.
Jumpathong J, Suphrom N, Dell B. Screening of antibacterial activity of Goniothalamus calvicarpa extracts against Xa
nthomonas axonopodis pv. citri in vitro. CMU J Nat Sci. 2020; 19(2):252-264.
Tangjitjaroenkun J, Tangchitcharoenkhul R, Yahayo W, Supabphol R. In vitro antimicrobial and cytotoxic activities of
mangrove actinomycetes from Eastern Thailand. Chiang Mai J Sci. 2017; 44(2):322-337.
Ranpariya B, Salunke G, Karmakar S, Babiya K, Sutar S, Kadoo N, Kumbhakar P, Ghosh S. Antimicrobial synergy of s
ilver-platinum nanohybrids with antibiotics. Front Microbiol. 2021; 11(2):1-12.
Ninkuu V, Adetunde LA, Sackey I, Opoku N, Diedong P. Antifungal efficacy of crude extracts of Azadirachta indica an
d Vernonia amygdalina against pathogenic Aspergillus niger (ATCC 16404). J Med Plants Res. 2019; 13(17):408-412.
Gowdhami T, Rajalakshmi AK, Sugumar N, Valliappan R. Evaluation of antimicrobial activity of different solvent extr
acts of aromatic plant: Jasminum sambac linn. J Chem Pharm Res. 2015; 7(11):136-143.
Barry A, Enade I, Maywan H. Future molecular medicine from white frangipani (Plumeria alba L.): a review. J Med Pl
ants Res. 2020; 14(10):544-554.
Shekhar S and Prasad MP. Comparative analysis of antioxidant properties of jasmine species by hydrogen peroxide assay. Eur J Biotechnol Biosci. 2015; 3(2):26-29.
Senbagam D, Senthilkumar B, Amutha R, Arunt, Nagarajan G, Kalandar A. Phytotherapeutic control of foodborne pathogens by Jasminum sambac L. Flowers. Int J Pharm Pharm Sci. 2016; 8(3):188-193.
Adamczak A, Ożarowski M, Karpiński TM. Antibacterial activity of some flavonoids and organic acids widely distribu
ted in plants. J Clin Med. 2019; 9,109:1-17.
Sagar NA and Pareek S. Antimicrobial assessment of polyphenolic extracts from onion (Allium cepa L.) skin of fifteen
cultivars by sonication-assisted extraction method. Heliyon. 2020; 6:e05478.
Joshi S, Amatya S, Pandey RD, Khadka P, Bhattarai J. Antimicrobial, antioxidant, antidiabetic, cytotoxic activities and
GC-MS analysis of methanolic extract of Elaeocarpus sphaericus leaves from Nepal. Int J Adv Res Chem Sci. 2020; 8(1):11-23.
Ezeonu IM and Chukwudozie IK. Antimicrobial activity, phytochemistry and acute toxicity profile of Sarcocephalius la
tifolius root bark. Trop J Nat Prod Res. 2021; 5(3):576-581.
Sharma N, Samarakoon KW, Gyawali R, Park YH, Lee SJ, Oh SJ, Lee TH, Jeong DK. Evaluation of the antioxidant, anti-inflammatory, and anticancer activities of Euphorbia hirta ethanolic extract. Molecules. 2014; 19(9):14567-14581.
Olivia NU, Goodness UC, Obinna OM. Phytochemical profiling and GC-MS analysis of aqueous methanol fraction of
Hibiscus asper leaves. Futur J Pharm Sci. 2021; 7(59):1-5.
Daood U, Matinlinna JP, Pichika MR, Mak KK, Nagendrababu V, Fawzy AS. A quaternary ammonium silane antimicr
obial triggers bacterial membrane and biofilm destruction. Sci Rep. 2020; 10(1):1-14.
Kok ESK, Lim XJ, Chew SX, Ong SF, See LY, Lim SH, Wong LA, Davamani F, Nagendrababu V, Fawzy A, Daood U
. Quaternary ammonium silane (k21) based intracanal medicament triggers biofilm destruction. BMC Oral Health. 202
; 21(1):1-17.
Abubakar M and Majinda R. GC-MS analysis and preliminary antimicrobial activity of Albizia adianthifolia (Schumac
h) and Pterocarpus angolensis (DC). Med. 2016; 3(3):1-9.
Hussain HY, Osama M, Hussain W. Stereostructure, antimicrobial and cytotoxic activity of cyclohexene, cyclohexanol
and pyridine derivatives synthesized from chalcones. Int J Pharm Sci Res. 2014; 5(5):2084-2094.
Mamedov IG, Shikhaliyevaa IM, Mamedova YA, Maharramov AM. Antibacterial and antifungal activities of thiosemi
carbazone, pyrazole, indole, cyclohexanol derivatives of 2-hydroxy-5-methyl- and 4-bromoacetophenones. Indian J Chem -Section B. 2019; 58(08):930-934.
Mamedov IG and Mamedova YV. Biological activity of novel pentasubstituted cyclohexanol against some icroorgani
sms. Indian J Chem - Sect B Org Med Chem. 2021; 60 B(2):283-286.
Shafie N ‘Izzati, Samsulrizal N, Sopian NA, Rajion MA, Meng GY, Ajat MMM, et al. Qualitative phytochemical scree
ning and GC-MS profiling of Azadirachta excelsa leaf extract. Malay Appl Biol. 2015; 44(3):87-92.
Iwara IA, Igile GO, Uboh FE, Eteng MU, Ebong PE. Analysis of bioactive constituents of n-hexane and methanol fracti
ons of Vernonia calvoana extracts using gas chromatography-mass spectrometry. Pharmacogn J. 2018; 10(5):854-858.
Bondaryk M, Staniszewska M, Zielińska P, Urbańczyk-Lipkowska Z. Natural antimicrobial peptides as inspiration for
design of a new generation antifungal compounds. J Fungi. 2017; 3(46):1-36.
Kim BR, Kim HM, Jin CH, Kang SY, Kim JB, Jeon YG, Park KY, Lee IS, Han AR. Composition and antioxidant activities of volatile organic compounds in radiation-bred coreopsis cultivars. Plants. 2020; 9(717):1-9.
Casiglia S, Bruno M, Rosselli S, Senatore F. Chemical composition and antimicrobial activity of the essential oil from f
lowers of Eryngium triquetrum (Apiaceae) collected wild in sicily. Nat Prod Commun. 2016; 11(7):1019-1024.
Yildiz H. Chemical composition, antimicrobial, and antioxidant activities of essential oil and ethanol extract of Corian
drum sativum L. leaves from Turkey. Int J Food Prop. 2016;19(7):1593-1603.
Pragadheesh VPPS, Yadav A, Chanotiya CS, Rout PK, Uniyal GC. Monitoring the emission of volatile organic compo
unds from flowers of Jasminum sambac using solid-phase micro-extraction fibers and gas chromatography with mass spectrometry detection. Nat Prod Commun. 2011; 6(9):1333-1338.
Khidzir KM, Cheng SF, Chuah CH. Interspecies variation of chemical constituents and antioxidant capacity of extracts
from Jasminum sambac and Jasminum multiflorum grown in Malaysia. Ind Crops Prod. 2015; 74:635-641.
Mahmood MA, Saeed M, Ahmad N. Quantitative and qualitative analysis of essential oil of Arabian jasmine (Jasminum sambac) flowers harvested from Pothohar region of Pakistan. J Ornam Plants. 2017; 7(1):17-24.
Rassem HH, Nour AH, Yunus RM. Analysis of bioactive compounds for jasmine flower via Gas chromatography-mass
spectrometry (GC-MS). Malay J Fundam Appl Sci. 2018; 14(2):198-201.
Bera P, Kotamreddy JNR, Samanta T, Maiti S, Mitra A. Inter-specific variation in headspace scent volatiles compositio
n of four commercially cultivated jasmine flowers. Nat Prod Res. 2015; 29(14):1328-1335.
Lawal OA, Ogunwande IA, Opoku AR. Constituents of essential oils from the leaf and flower of Plumeria alba grown
in Nigeria. Nat Prod Commun. 2014; 9(11):1613-1614.
Anand U, Nandy S, Mundhra A, Das N, Pandey DK, Dey A. A review on antimicrobial botanicals, phytochemicals and
natural resistance modifying agents from Apocynaceae family: possible therapeutic approaches against multidrug resistance in pathogenic microorganisms. Drug Resist Updat. 2020; 51(100695):1-30.
Mohamad SA, Adzahar NS, Akhtar MN, Zareen S, Lee TC. Phytochemical analysis and GC-MS profiling in the flower
of Plumeria alba. Mater Sci Forum. 2020; 981:280-284.
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