Investigating the Effect of Flavonoid, Saponin, Alkaloids and Tannins Extracted from Combretum dolichopentalum Diels in CCl4 –Induced Hepatotoxicity

doi.org/10.26538/tjnpr/v6i8.16

Authors

  • Favour N.Ujowundu Federal University of Technology Owerri, Imo State Nigeria
  • James O. Kalu Federal University of Technology Owerri, Imo State Nigeria
  • Cosmas O. Ujowundu Federal University of Technology Owerri, Imo State Nigeria
  • Ignatius O. Onyeocha Federal University of Technology Owerri, Imo State Nigeria
  • Chinyere H. Onuoha Federal University of Technology Owerri, Imo State Nigeria
  • Raymond C. Ibeh Federal University of Technology Owerri, Imo State Nigeria
  • Uchechi K. Obasi Federal University of Technology Owerri, Imo State Nigeria
  • Oluwatosin E. Ntaji Federal University of Technology Owerri, Imo State Nigeria
  • Ifeyinwa N. Chigbu Federal University of Technology Owerri, Imo State Nigeria
  • Chioma Y. Ezirim Federal University of Technology Owerri, Imo State Nigeria

Keywords:

Combretum dolichopentalum, Flavonoids,, Saponins,, Tannins,, Hepatotoxicity.

Abstract

Modification of biological molecules caused by oxidative stress is the most popular harmful b side reaction leading to pathological changes. This study investigated the impact of flavonoids, a saponins, and tannin extracted from Combretum dolichopentalum on CCl4-induced hepatotoxicit five male rats were sorted into nine groups, and allowed access to water and food. Groups received distilled water only, groups III and IV pre-treated with 250 and 500 mg/kg body wei ethanol extract of C. dolichopentalum (EECD) respectively, group V pre-treated with 50 m silymarin, group VI to IX pre-treated with 100 mg/kg bwt of flavonoids, saponins, alkaloids and C. dolichopentalum respectively. After seven days, all animals (except normal control) were in with 0.4 mL/kg bwt CCl4, and sacrificed 48 h after. Samples of liver and blood were used for and biochemical studies respectively. Results showed that CCl4   -induced  massive fatty cha centrilobular necrosis in the liver, marked elevations in serum malondialdehyde (MDA), live enzymes  and  other  biochemical  changes  were  observed.  However,  animals  administered alkaloids, flavonoids, tannins and saponins of C. dolichopentalum presented improved liver his feature.  The  biochemical  parameters  of  treated  rats  approached  that  of  normal  control  w administered alkaloid and flavonoid showed more significant decrease in MDA and total biliru results from the current study show that C. dolichopentalum, especially flavonoid and alkaloid clearly protects the liver from CCl4 induced hepatotoxicity. Furthermore, the saponin fracti dolichopentalum appears to be hepatotoxic to the liver.

References

Clarke S. Ageing as a war between chemical and biochemical processes: protein methylation and the recognition of age-damaged proteins for repair. Ageing Res Rev. 2003; 2(3):263-285.

Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. Oxidative Stress: Harms and Benefits for Human Health. Oxid Med Cell Longev. 2017;2017:8416763:1-13.

Kietzmann T and Gorlach A. Reactive oxygen species in the control of hypoxia-inducible factor-mediated gene expression. Semin Cell Dev Biol. 2005; 16(4-5):474-486.

Tan BL, Norhaizan ME, Liew WPP Sulaiman RH. Antioxidant and Oxidative Stress: A Mutual Interplay in Age-Related Diseases. Front Pharmacol. 2018; 9:1162: 1-28.

Asuzu IU and Onu OU. Anti-ulcer activity of the Ethanol Extract of Combretum dolichopentalum Root. J Crude Drug Res. 1988; 25:44 – 48.

Ujowundu FN, Ukoha AI, Ojiako AO, Nwaoguikpe RN. Nutritional characterization of Combretum dolichopentalum Leaves. Biochem Anal Biochem. 2015; 4(4):1-5.

Ujowundu FN. In vitro evaluation of free radical-scavenging potentials of ethanol extract of Combretum dolichopentalum leaves. Glob Drugs Ther.2017; 2(6):1-5.

Ujowundu FN, Ukoha AI, Ojiako AO, Nwaoguikpe RN. Gas chromatographic characterization of the flavonoids, alkaloids, saponins, and tannins isolated from C. dolichopentalum leaves. J Chem Pharm Res. 2015; 7(12):1094-1103.

Ujowundu FN, Ojiako AO, Nwaoguikpe RN, Ujowundu CO. Gas Chromatography-Mass Spectrometry and Infra-Red Studies of Bioactive Phytoorganic Components of Combretum dolichopentalum Leaves. Int J Drug Dev Res. 2017; 9:10-15.

Ujowundu FN. Determination of Antimicrobial Potentials of Ethanol Extract of Combretum dolichopentalum Leaves by Total Dehydrogenase Activity Assay. Int J Pharmacol Phytochem Ethnomed. 2017;8:27-40.

Ujowundu FN, Oparaeche NN, Ujowundu CO, Nwachukwu IN, Nwobodo AM, Enomfon OU. Methanol Extract of Combretum dolichopentalum Exhibits Broad-spectrum Antimicrobial Effect on Nosocomial Organisms. Asian J Biol Sci. 2019; 12:557-564.

Ujowundu CO, Onyema CR, Nwachukwu N, Ujowundu FN, Onwuliri VA, Igwe KO, Achilike JJ, Udensi JU. Antioxidative Effect of Phenolic Extract of Vitex doniana Leaves on Alloxan-Induced Diabetic Stress and Histological Changes in the Pancreas of Wistar Rat. Trop J Nat Prod Res. 2022; 6(2):270-275.

Unsal V, Cicek M, Sabancilar İ. Toxicity of carbon tetrachloride, free radicals and role of antioxidants. Rev Environ Health. 2021; 36(2):279-295.

Obadoni PO and Ochuko MC. Practical methods of determining various components from plant extract. Adv Environ Med Biol. 2001;102:341-398.

Boham BA and Kocipai-Abyazan R. Flavonoids and condensed tannins from leaves of Hawaiian vaccinium vaticulatum and V. calycinium. Pac Sci. 1974; 48:458-463.

National Institutes of Health. Guide for the Care and Use of Laboratory Animals. NIH Publication Number 85-23, US Department of Health, Education and Welfare, Bethesda, MD. 1985.

Okoro I. Manuel of Pratical of Histology. (2nd ed.). Owerri. Imo State: Peace Publishers; 2002; 13-24p.

Xin Z, Waterman DF, Henken RM, Harmon RJ. Effects of Copper status on neutrophil function, Superoxide dismutase and Copper distribution in Steers. J Diary Sci. 1991; 9(74):3078-3085.

Reitman S and Frankel S. A Colorimetric method of determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J ClinPathol. 1957; 28(1):56-63.

Wallin B, Rosengren B, Shetzer HG, Camejo G. Lipoprotein oxidation and measurement of thiobarbituric acid reacting substances (TBARS) formation in a single microlitre plate: its use for evaluation of antioxidants. Anal Biochem. 1993; 208(1):10-15.

Tietz NW. Clinical guide to Laboratory Test. (3rd ed.). Philadelphia: W.B. Sunders Company; 1995; 518-519p.

Doumas BT, Watson WA, Biggs HG. Albumin standards’ and the measurement of serum albumin with bromocresol green. ClinChimActa. 1971; 31(1):87-96.

Zoppi FPA, Felini D, Marcovina S, Ramella C. Method for the determination of total and conjugate bilirubin. Use of a cationic surfactant as a solubilizing agent. Italian Clin Chem Day. 1976; 1:343-359.

Ujowundu FN, Oparaeche NN, Onuoha CH, Haruna MA, Chieme CS, Ujowundu CO. Combretum dolichopentalum extract normalized biochemical and haematological parameters in carbon tetrachloride (CCL4) intoxicated rats. AROC Nat Prod Res. 2021; 1(2):17-28.

Yang C, Lin Y, Liu K, Peng W, Hsu C. Hepatoprotective Mechanisms of Taxifolin on Carbon Tetrachloride-Induced Acute Liver Injury in Mice. Nutrients. 2019; 11(11):2655.

Michael M, Gaschler B, Stockwell R. Lipid peroxidation in death. Biochem Biophys Res Commun. 2017; 482(3):419- 425.

Abdel-Kader MS, Abulhamd AT, Hamad AM, Alanazi AH, Ali R, Alqasoumi SI. Evaluation of the hepatoprotective effect of combination between hinokiflavone and Glycyrrhizin against CCl4 induced toxicity in rats. Saudi Pharm J. 2018; 26(4):496-503.

Ujowundu CO, Kalu FN, Nwaoguikpe RN, Okechukwu RI, Ihejirika CE. The antioxidative potentials of Gongronema latifolium on diesel petroleum inducedhepatotoxicity. J Appl Pharm. 2012; 2(1):90-94.

Kowalczyk , Sulejczak D, leczkowska , Bukowska- O sko I, ucia M, opiel M, Wietrak E, ramkowski , Wrzosek , aczy nska . Mitochondrial Oxidative Stress—A Causative Factor and Therapeutic Target in Many Diseases. Int J Mol Sci. 2021; 22(24):13384.

Novaes RD, Goncalves RV, Cupertino MC, Santos EC, Bigonha SM. Acute paraquat exposure determines dose- dependent oxidative injury of multiple organs and metabolic dysfunction in rats: impact on exercise tolerance. Int J ExpPathol. 2016; 97(2):114-124.

Elsawy H, Badr GM, Sedky A, Abdallah BM, Alzahrani AM, Abdel-Moneim AM. Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male rats. Peer J. 2019; 7:e7011.

Chukwudoruo CS, Osuji-Kalu-Ibe NC, Igwe KO, Iheme CI, Ujowundu FN, Mba BA. Serum total protein concentration and liver enzymes activities in albino rats model administered with ethanolic leaf extract of Ficus capensis. Afr J Biotechnol. 2021; 20(4):164-168.

Dutta S, Chakraborty AK, Dey P, Kar P, Guha P, Sen S, Kumar A, Sen A, Chaudhuri TK. Amelioration of CCl4 induced liver injury in swiss albino mice by antioxidant rich leaf extract of Croton bonplandianus Baill. PLoS ONE 2018; 13(4):e0196411.

Li XW, Zhu R, Li B, Zhou M, Sheng QJ, Yang YP, Han NY, Li ZQ. Mechanism underlying carbon tetrachloride-inhibited protein synthesis in liver. World J Gastroenterol. 2010; 16(31):3950-3956.

Chatterjea MN and Shinde R. Text Book of Medical Biochemistry. (7th ed.). New Delhi: Jaypee Brothers Medical Publishers; 2007; 93-674p.

Sumaiya S, Naved T, Sharma A, Sarwat M. Chapter 1 - Amelioration of Liver Ailments by Saffron (Crocus sativus) and Its Secondary Metabolites, In: Maryam S, Sajida S. Saffron. Academic Press; 2020; 1-20p.

Ujowundu FN, Ujowundu CO, Ibeh CR, Iweala EJ, Onuoha CH, Iheme C I, Chukwudoruo SC, Kalu JO, Okorondu MM, Haruna MA. Amelioration of CCl4 –induced Nephrotoxicity in rat by flavonoid, alkaloids, saponin, and tannins extracted from Combretum dolichopentalum. Int J Mod Pharm Res. 2022; 6(3):01-11.

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Published

2022-08-01

How to Cite

N.Ujowundu, F., O. Kalu, J., O. Ujowundu, C., O. Onyeocha, I., H. Onuoha, C., C. Ibeh, R., … Y. Ezirim, C. (2022). Investigating the Effect of Flavonoid, Saponin, Alkaloids and Tannins Extracted from Combretum dolichopentalum Diels in CCl4 –Induced Hepatotoxicity: doi.org/10.26538/tjnpr/v6i8.16. Tropical Journal of Natural Product Research (TJNPR), 6(8), 1255–1261. Retrieved from https://tjnpr.org/index.php/home/article/view/1298

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