HPLC Fingerprinting and In vitro Antimycobacterial Activity of the Roots of Cissampelos owariensis and Cissampelos mucronata



  • Nneka N. Ibekwe Department of Medicinal Chemistry and Quality Control, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria
  • Tiwalade A. Adelakun Department of Medicinal Chemistry and Quality Control, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria
  • , Kasim S. Izebe Department of Microbiology and Biotechnology, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria
  • Obi P. Adigwe Office of the Director-General, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria


Cissampelos owariensis, Cissampelos mucronata,, Antimycobacterial,, HPLC,, Quality control.


Two Nigerian medicinal plants Cissampelos owariensis and Cissampelos mucronata (Menispermaceae) are commonly used in traditional medicine for the management of tuberculosis-related symptoms. The rationale behind this study is based on the fact that the two plants possess similar appearances thus often mistaken for each other, and both have also been reported for antimycobacterial activity. Hence, the objective of this study was to profile the chemical constituents of the two plants, establish their respective chromatographic fingerprints as an identity marker, and compare their bioactivities against two Mycobacterium species. Aqueous methanol extract of the roots of both plants was screened for their secondary metabolite contents and were also evaluated for their action against Bacillus Camille Guerin and Mycobacterium smegmatis. Alkaloids, flavonoids, steroids and terpenes were present in both plant extracts. In-vitro antimycobacterial assay showed that the extracts of C. owariensis and C. mucronata inhibited the growth of M. smegmatis at 3.13 mg/mL and 6.25 mg/mL, respectively and was bactericidal at 6.25 mg/mL and 12.5 mg/mL, respectively. Against BCG, the extract of owariensis displayed inhibitory and bactericidal properties at 0.39 mg/mL and 0.78 mg/mL, respectively, and C. mucronata at 3.13 mg/mL and 6.25 mg/mL, respectively. This indicates that both strains of the Mycobacterium were more susceptible to C. owariensis than C. mucronata in- vitro. The HPLC fingerprint results were non-identical for the two plant extracts, and comparison with their alkaloid fraction chromatograph revealed that the crude extracts consisted largely of alkaloids. This study has established distinguishable chromatographic profiles between the two species.


World Health Organization. Global Tuberculosis report 2021[Internet]. Geneva, Switzerland: WHO; 2021. Available from https://www.who.int/publications/i/item/9789240037021 (Accessed 2022 April 25).

Allué-Guardia A, García JI, Torrelles JB. Evolution of drug- resistant Mycobacterium tuberculosis strains and their adaptation to the human lung environment. Front Microbiol. 2021;12:612675.

Ibekwe NN, Nvau JB, Oladosu PO, Usman AM, Ibrahim K, Boshoff HI, Dowd CS, Orisadipe AT, Aiyelaagbe O, Adesomoju AA, Barry III CE. Some Nigerian anti-tuberculosis ethnomedicines: a preliminary efficacy assessment. J Ethnopharmacol. 2014;155(1):524‒532.

Muzila M. Cissampelos mucronata. In: Schmelzer GH, Gurib- Fakim A. (Eds.) Plant resources of tropical Africa 11: Medicinal plants I. Leiden, Netherlands: Backhuys Publishers; 2006. 174‒ 176 p.

Aska A, Kubmarawa D, Nkafamiya I, Shagal H, Oladosu P. Quantitative phytochemical analysis and anti-tuberculosis activity of some selected medicinal plants in some Northern parts of Bauchi state, Nigeria. IOSR J Appl Chem. 2019;12(6):15‒22.

Akande R, Okwute SK, Iliya I, Efiom OO. Chemical constituents and anti-tuberculosis activity of the root extracts of Cissampelos owariensis (P. Beauv.) Menispermaceae. Afr J Pure Appl Chem. 2013;7(1):21‒30.

Efunsayo RO, Olagbende-Dada SO, Mohammed AH. Aqueous leaf extract of Cissampelos owariensis (P.Beauv) Menispermaceae exerts tocolytic activity on isolated gravid rat uterus. Trop J Nat Prod Res. 2018;2(7):354‒357.

Earnest EO, Goodies MO, Paul C. Cissampelos owariensis: Experimental review. Pharm Innov J. 2015;3(11):75‒77.

Akoègninou A, van der Burg WJ, van der Maesen LJG. Analytical flora of Benin. Leiden, Netherlands: Backhuys Publishers; 2006. 1034 p.

Lawal IO, Olufade II, Rafiu BO, Aremu AO. Ethnobotanical survey of plants used for treating cough associated with respiratory conditions in Ede South local government area of Osun State, Nigeria. Plants. 2020;9(5):647.

Efiom O. Isolation and characterization of bis (2–Methoxyethyl) phthalate and hexahydro-1 3–dimethyl–4–phenyl–1H–azepine 4– carboxylic acid from the root of Cissampelos owariensis (P. Beauv). Nig J Basic Appl Sci. 2010;18(2):189‒192.

Sparg S, Van Staden J, Jäger A. Efficiency of traditionally used South African plants against schistosomiasis. J Ethnopharmacol. 2000;73(1‒2):209‒214.

Tshibangu JN, Chifundera K, Kaminsky R, Wright AD, König GM. Screening of African medicinal plants for antimicrobial and enzyme inhibitory activity. J Ethnopharmacol. 2002;80(1):25‒35.

Gessler M, Tanner M, Chollet J, Nkunya M, Heinrich M. Tanzanian medicinal plants used traditionally for the treatment of malaria: in vivo antimalarial and in vitro cytotoxic activities. Phytother Res. 1995; 9(7):504‒508.

Van Zyl R, De Wet H, van Wyk B, Van Heerden F. Antimalarial activity of thirteen South African Menispermaceae species. Planta Med. 2009;75(09):11.

Akah P, Nwafor S, Okoli C, Egbogha C. Evaluation of the sedative properties of the ethanolic root extract of Cissampelos mucronata. Boll Chim Farm. 2002;141(3):243-246.

Taniguchi M, Chapya A, Kubo I, Nakanishi K. Screening of East African plants for antimicrobial activity. I. Chem Pharm Bull. 1978; 26(9):2910‒2913.

Nondo RS, Mbwambo ZH, Kidukuli AW, Innocent EM, Mihale MJ, Erasto P, Moshi MJ. Larvicidal, antimicrobial and brine shrimp activities of extracts from Cissampelos mucronata and Tephrosia villosa from coast region, Tanzania. BMC Compl Altern Med. 2011;11(1):1‒7.

Nwafor S, Akah P, Okoli C, Ndu O, Ichu E. Uterine relaxant property of the ethanolic root extract of Cissampelos mucronata. J Nat Rem. 2002;2(1):59‒65.

Nwafor S and Okoye C. Antiulcer properties of the ethanol root extract of Cissampelos mucronata. Pharm Biol. 2005;43(5):396‒ 403.

Kela S, Ogunsusi R, Ogbogu V, Nwude N. Screening of some Nigerian plants for molluscicidal activity. Rev Elev Med Vet Pays Trop. 1989;42(2):195‒202.

McGaw L, Lall N, Meyer J, Eloff J. The potential of South African plants against Mycobacterium infections. J Ethnopharmacol. 2008;119(3):482‒500.

Si Y, Ding X, Adelakun TA, Zhang Y, Hao XJ. Acotarines A-G, new diterpenoid alkaloids from Aconitum taronense induce lysosomal biogenesis. Fitoterapia. 2020;147:104738.

Khalid S, Shahzad A, Basharat N, Abubakar M, Anwar P. Phytochemical screening and analysis of selected medicinal plants in Gujrat. J Phytochem Biochem. 2018;2:108.

Caviedes L, Delgado J, Gilman RH. Tetrazolium microplate assay as a rapid and inexpensive colorimetric method for determination of antibiotic susceptibility of Mycobacterium tuberculosis. J Clin Microbiol. 2002;40(5):1873‒1874.

Ncube NS, Afolayan AJ, Okoh AI. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. Afr J Biotechnol. 2008;7(12):1797‒1806.

Baron EJ, Peterson, LR, Finegold SM. Baily & Scotts diagnostic microbiology (9th ed.). Toronto: Mosby; 1994. 168‒187 p.

Alaerts G, Van Erps J, Pieters S, Dumarey M, Van Nederkassel AM, Goodarzi M, Smeyers-Verbeke J, Vander Heyden Y. Similarity analyses of chromatographic fingerprints as tools for identification and quality control of green tea. J Chromatogr B. 2012;910: 61‒70.

Goodarzi M, Russell PJ, Vander Heyden Y. Similarity analyses of chromatographic herbal fingerprints: a review. Anal Chim Acta. 2013; 804:16‒28.

Cahya S, Irmanida B, Mohamad R. HPLC Fingerprint analysis combined with chemometrics for authentication of Kaempferia galanga from related species. Indones J Chem. 2016;16(3):308– 314.

Maroyi A. A Synthesis and review of medicinal uses, phytochemistry and pharmacological properties of Cissampelos mucronata A. Rich.(Menispermaceae). J Pharm Nutr Sci. 2020;10:213‒222.

Dandjesso C, Klotoé J, Dougnon T, Sègbo J, Atègbo J, Gbaguidi F, Fah L, Fanou B, Loko F, Dramane K. Phytochemistry and hemostatic properties of some medicinal plants sold as anti- hemorrhagic in Cotonou markets (Benin). Indian J Sci Technol. 2012;5(8):3105‒3109.

Tshibangu JN, Wright AD, König GM. HPLC isolation of the anti‐plasmodially active bisbenzylisoquinone alkaloids present in roots of Cissampelos mucronata. Phytochem Anal. 2003;14(1):13‒22.

Mendes JW, Cunha WEM, Rodrigues FFG, Lima RDP, Costa JGM. Cissampelos genus: biological activities, ethnobotanical and phytochemical aspects. Phytochem Rev. 2020; 19:955–982.




How to Cite

N. Ibekwe, N., A. Adelakun, T., S. Izebe, , K., & P. Adigwe, O. (2022). HPLC Fingerprinting and In vitro Antimycobacterial Activity of the Roots of Cissampelos owariensis and Cissampelos mucronata: doi.org/10.26538/tjnpr/v6i8.15. Tropical Journal of Natural Product Research (TJNPR), 6(8), 1249–1254. Retrieved from https://tjnpr.org/index.php/home/article/view/1297