Acute and Subchronic Toxicity Study of Methanol Extract of the Aerial Parts of Ruta montana L. on Adult Female Wistar Rats


  • Ghedjati Nadra Department of Biochemistry, Faculty of natural sciences and life, University Ferhat Abbas 1, Setif, Algeria
  • Mahdeb Nadia Department of Biochemistry, Faculty of natural sciences and life, University Ferhat Abbas 1, Setif, Algeria
  • Bouzidi A. El Ouahab Department of Biochemistry, Faculty of natural sciences and life, University Ferhat Abbas 1, Setif, Algeria


Ruta montana L.,, Female rats,, LD50,, Subchronic toxicity,, Reproductive hormone


Ruta montana L. locally known as fidjel belongs to the rutaceae family. This plant is used in traditional medicine as an aphrodisiac, abortive and as hypoglycemic. The aim of this research is to evaluate the mean lethal dose (LD50) and subchronic toxicity of methanol extract of Ruta montana L. on female rat. To determine the LD50, 30 female rats were divided into 5 groups; each group received intraperitoneally, a single dose (800, 1000, 1200, 1400 and 1600 mg/kg bw) of the extract, toxicity symptoms and mortality were registered for 14 consecutive days. In subchronic toxicity (45 days), 14 female rats were divided into 2 groups; treated group received intraperitoneally 38,7149 mg/kg bw = 1/30 LD50 of the extract and control group received physiological water. Body weight, relative organ masses, reproductive  hormones, haematological, biochemical and histopathological parameters were evaluated. The LD50 value was calculated to be 1161.4486 mg/kg by Litchfield and Wilcoxon method. In the subchronic study, there was a significant reduction in body weight in the 2nd and 7th week (P<0.05). Relative mass  of  the  brain  and  ovaries  registered  a  significant  decrease  and  increase,  respectively(P<0.05). Follicle stimulating hormone (FSH), luteinizing hormone (LH) and biochemical parameters did not record significant difference. Haematological  parameters  presented significant increase and decrease in mean corpuscular haemoglobin (MCH) and red cell distribution width (RDW), respectively (P<0.05). The histopathology revealed no abnormalities in different studied tissues. The current study revealed that the methanol extract  of  Ruta montana at the tested dose had no toxic effect on subjected organs.


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How to Cite

Nadra, G., Nadia, M., & A. El Ouahab, B. (2022). Acute and Subchronic Toxicity Study of Methanol Extract of the Aerial Parts of Ruta montana L. on Adult Female Wistar Rats: Tropical Journal of Natural Product Research (TJNPR), 6(8), 1203–1209. Retrieved from