Diisooctyl Phthalate Isolated from Rourea mimosoides: A Partial PPARγ Agonist Potently Blocks Adipocyte Differentiation



  • Mohd I. Adenan Universiti Teknologi MARA Pahang Branch, Bandar Tun Abdul Razak 26400, Jengka, Pahang Darul Makmur, Malaysia
  • Tengku S. T. Muhammad Centre for Research and Innovation Management, Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Terengganu, Malaysia
  • Chuah B. Chung Malaysian Institute of Pharmaceutical and Nutraceutical (IPharm), Blok 5A Halaman Bukit Gambir, 11700 Pulau Pinang, Malaysia
  • Mohd A. Aizuddin Malaysian Institute of Pharmaceutical and Nutraceutical (IPharm), Blok 5A Halaman Bukit Gambir, 11700 Pulau Pinang, Malaysia
  • Zuriati Zahari Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam 40450, Selangor Darul Ehsan, Malaysia


Type II Diabetes mellitus, PPAR-γ,, Adipocyte Differentiation,, Rourea mimosoides,, Diisooctyl phthalate.


Type II diabetes mellitus (T2DM) is a metabolic disorder due to the development of insulin resistant caused by the modification of insulin signalling, resulting in a reduction in glucose uptake in myocytes, hepatocytes, adipocytes, and elevation of blood glucose levels. Thiazolidinediones (TZDs) are medications used in the treatment of T2DM. The primary target of TZDs is the peroxisome proliferator-activated receptor-gamma (PPAR-γ), a key regulator of adipogenesis and glucose homeostasis. In this study, extracts from Malaysian plants were tested in a screening platform which includes tests for adipocyte differentiation, insulin-stimulated glucose uptake and PPAR-γ transactivation. A total of 122 plant extracts collected from Royal Belum Rainforest, Malaysia were tested on adipocyte differentiation, glucose uptake and PPAR-transcription assays. Rourea mimosoides was found to inhibit the adipocyte differentiation and showed the ability to enhance the glucose uptake in C2C12 myoblast cells as well as active to promote the transcription of PPAR-γ. A bioactive compound designated as compound 1 was isolated from this plant and was identified as Diisooctyl phthalate. The biological activities (adipocyte differentiation and PPAR-γ transactivation) of this compound were demonstrated and compound 1 was found to possess the ability to trigger expression of PPAR-γ in HepG2 cells.


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How to Cite

I. Adenan, M., S. T. Muhammad, T., B. Chung, C., A. Aizuddin, M., & Zahari, Z. (2022). Diisooctyl Phthalate Isolated from Rourea mimosoides: A Partial PPARγ Agonist Potently Blocks Adipocyte Differentiation: doi.org/10.26538/tjnpr/v6i8.6. Tropical Journal of Natural Product Research (TJNPR), 6(8), 1197–1202. Retrieved from https://tjnpr.org/index.php/home/article/view/1287