Characterization of Crude, Bleached Rubber (Hevea brasiliensis) Seed Oil and Synthesis of its Methyl Ester Using Soy-Bean (Glycine max) Lipase as Biocatalyst http://www.doi.org/10.26538/tjnpr/v6i10.24
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Abstract
Biodiesel is one of the alternatives to fossil fuels derived from vegetables and plant sources. This paper reports biodiesel production from rubber seed oil (Hevea brasiliensis) via soybean lipase- catalyzed transesterification. 86.62% biodiesel and 27.21% glycerol yield were obtained at lipase activity of 24375 IU ascertained by the titrimetric method. 80% ammonium sulfate saturation was used to precipitate lipase. The pellet showed 376250 IU lipase loading, oil to methanol equivalent molar ratio of 5:1, and under 24 hours. The physicochemical properties of the crude, bleached oil, and biodiesel such as peroxide, acid, anisidine, saponification, and fatty acid values were analyzed. The flashpoints recorded were 80.5±1.5 °C, 97±5 °C, and 56±0 °C respectively; it is safer than fossil diesel since it is not ≤ 50ºC. The saponification values (SV) were 62.3, 61.7, and 103.8±2.4 mgKOH/g. Since little quantity of soap in biodiesel can minimize the friction occurring in moving parts and enhance fuel pump purity, it could be employed in running a compression engine. Enzymatic transesterification of rubber seed oil gives a high yield of biodiesel with robust properties suitable for automobile application.
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Winoto V and Yoswathana N. Optimization of biodiesel production using nanomagnetic CaO-based catalysts with subcritical methanol transesterification of rubber seed oil. Energies. 2019; 12(2):230.
Abas N, Kalair A, Khan N. Review of fossil fuels and future energy technologies. Futures. 2015; 69:31-49.
Giakoumis EG. A statistical investigation of biodiesel physical and chemical properties, and their correlation with the degree of unsaturation. Renewable Energy. 2013; 50:858-78.
Vilas Bôas RN, Mendes MF. A review of biodiesel production from non-edible raw materials using the transesterification process with a focus on influence of feedstock composition and free fatty acids. J Chilean Chem Soc. 2022; 67(1):5433-44.
Zieniuk B, Wołoszynowska M, Białecka-Florjańczyk E. Enzymatic synthesis of biodiesel by direct transesterification of rapeseed cake. Int J Food Engr. 2020; 16(3).
Wu L, Wei TY, Tong ZF, Zou Y, Lin ZJ, Sun JH. Bentonite- enhanced biodiesel production by NaOH-catalyzed transesterification of soybean oil with methanol. Fuel Proc Technol. 2016; 144:334-40.
Al‐Zuhair S. Production of biodiesel by lipase‐catalyzed transesterification of vegetable oils: A kinetics study. Biotechnol Prog. 2005; 21(5):1442-8.
Du W, Xu YY, Liu DH, Li ZB. Study on acyl migration in immobilized lipozyme TL-catalyzed transesterification of soybean oil for biodiesel production. Journal of Molecular Catalysis B: Enzy. 2005; 37(1-6):68-71.
Shah S, Sharma S, Gupta MN. Biodiesel preparation by lipase- catalyzed transesterification of Jatropha oil. Energy & Fuels. 2004; 18(1):154-9.
Ulfah M, Praputri E, Sundari E. Biodiesel production methods of rubber seed oil: a review. InIOP Conference Series: Mater. Sci and Engr 2018; 334(1):012006.
Rachmadona N, Amoah J, Quayson E, Hama S, Yoshida A, Kondo A, Ogino C. Lipase-catalyzed ethanolysis for biodiesel production of untreated palm oil mill effluent. Sustain Energy & Fuels. 2020; 4(3):1105-11.
Ikwuagwu OE, Ononogbu IC, Njoku OU. Production of biodiesel using rubber [Hevea brasiliensis (Kunth. Muell.)] seed oil. Industrial crops and products. 2000; 12(1):57-62.
American Oil Chemists' Society, Link WE. Official and tentative methods of the American Oil Chemists' Society. The Society; 1971.
Kumar D, Pugazhendi A, Bajhaiya AK, Gugulothu P. Biofuel production from Macroalgae: present scenario and future scope. Bioengineered. 2021; 12(2):9216.
Diamond, Paul S, Ronald FD. Laboratory Techniques in Chemistry and Biochemistry. No. QD45 D49. 1966.
Aguieiras EC, Cavalcanti-Oliveira ED, de Castro AM, Langone MA, Freire DM. Biodiesel production from Acrocomia aculeata acid oil by (enzyme/enzyme) hydroesterification process: use of vegetable lipase and fermented solid as low-cost biocatalysts. Fuel. 2014; 135:315-21.
Nehdi I, Omri S, Khalil MI, Al-Resayes SI. Characteristics and chemical composition of date palm (Phoenix canariensis) seeds and seed oil. Ind crops and products. 2010; 32(3):360-5.
Reshad AS, Tiwari P, Goud VV. Extraction of oil from rubber seeds for biodiesel application: Optimization of parameters. Fuel. 2015; 150:636-44.
Iko W and Eze SO. Physicochemical characterization of Quassia undulata seed oil for biodiesel production. Afri J Biotechnol. 2012; 11(83):14930-3.
Ved K and Padam K. Study of physical and chemical properties of biodiesel from sorghum oil. Res J Chem Sci. 2013; 3(9):64-8.
Rajesh K, Ram P. Production, physico-chemical and cold-flow properties of biodiesel from Jatropha and Karanja oils. Res J Chem Sci. 2014; 4(12):9-12.
Hideki F, Akihiko K, Hideo N. Biodiesel fuel production by transesterification of oils. J biosci and bioengin. 2001; 92(5):405-16.
Ulfah M, Praputri E, Sundari E. Biodiesel production methods of rubber seed oil: a review. InIOP Conference Series: Mat Sci and Engin 2018; 334(1):012006.
Adebayo GB, Ameen OM, Abass LT. Physico-chemical properties of biodiesel produced from Jatropha curcas oil and fossil diesel. J Microbiol and Biotechnol Res. 2011; 1(1):12-6.
Njoku OU and Ononogbu IC. Preliminary studies on preparation of lubricating greases from bleached rubber seed oil. Ind. J. NR. Res. 1995; 8(20):140-1.
Ebewele RO, Iyayi AF, Hymore FK. Considerations of the extraction process and potential technical applications of Nigerian rubber seed oil. Int J Phy sci. 2010; 5(6):826-831.
Uriate FA. Biofuels from Plant Oils: A book for practitioners and professionals involved in biofuels, to promote a better and more accurate understanding of the nature, production and use of biofuels from plant oils. National Academy of Science and Technology. Government of Japan. Japan ASEAN Solidarity Fund. 2010.
Singh D, Sharma D, Soni SL, Sharma S, Kumari D. Chemical compositions, properties, and standards for different generation biodiesels: A review. Fuel. 2019; 253:60-71.
Kalayasiri P, Jeyashoke N, Krisnangkura K. Survey of seed oils for use as diesel fuels. J American Oil Chemists’ Soci. 1996; 73(4):471-4.
Ramos MJ, Fernández CM, Casas A, Rodríguez L, Pérez Á. Influence of fatty acid composition of raw materials on biodiesel properties. Bioresource technol. 2009; 100(1):261-8.
Huang Z, Huang J, Luo J, Hu D, Yin Z. Performance enhancement and emission reduction of a diesel engine fueled with different biodiesel-diesel blending fuel based on the multi- parameter optimization theory. Fuel. 2022; 314:122753.