In vitro Assessment of Cytotoxic Potential of Ajuga orientalis L. Methanol Extract on MCF-7and MRC-5 cells
http://www.doi.org/10.26538/tjnpr/v6i9.11
Keywords:
MRC-5, MCF-7, MTT, Cytotoxic activity, Lamiaceae, Ajuga orientalis LAbstract
Cancer is a significant public health concern both globally and in Jordan. Modern cancer therapies are associated with multidrug resistance, severe side effects, and high costs. There has recently been a lot of interest in screening for plants that can be used in cancer prevention and treatment. The current study is intended to assess the cytotoxic activity of methanolic extract of aerial parts of Ajuga orientalis L. on a human breast cancer cell line (MCF-7). The cytotoxic activity of the extract against human breast cancer cells (MCF-7) and normal fibroblast (MRC-5) was assessed using the (3-(4,5- dimethylthiazol-2-yl) - 2,5- diphenyltetrazolium bromide
(MTT) assay. The cells were treated with different concentrations of methanol extract of Ajuga orientalis (3.125-200 μg/mL) for 24, 48, and 72 hrs. The extract demonstrated inhibition in the proliferation of the MCF-7 cell line in a dose-dependent manner, without any toxic impact on normal fibroblast cells. Furthermore, the extract showed significant cytotoxic activity after 72 hrs of incubation (IC50 30.5±0.05 μg/mL). In conclusion, the methanol extract of A. orientalis showed a potent reduction in the proliferation of MCF-7 cells and could be considered a potential source of the anticancer compound.
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