Comparative Study, Phytochemical Screening and Antioxidant Activities of Three Types of Apple Seed Extracts http://www.doi.org/10.26538/tjnpr/v6i9.9
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Abstract
The “Red Delicious, Golden Delicious, and Granny Smith” apples are widely cultivated and consumed worldwide. Because of their therapeutic phytochemical content, research findings have confirmed their beneficial effects in disease prevention and management. The present study was aimed at determining the phytochemical profile and evaluating the antioxidant activity of the seed extract of three varieties of apple. Water, methanol, chloroform, and hexane were used as solvents to extract the seeds of the three varieties of apples. The three crude apple seed extracts were subjected to preliminary phytochemical screening and antioxidant activity testing
using 2,2-diphenyl-1-picrylhydrazyl (DPPH). The obtained extracts tested positive for alkaloid, flavonoid, saponin, and tannin compounds in phytochemical screening. However, no glycosides or anthraquinones were observed in any of the extracts. The outcome of the antioxidant capacity test for all the varieties of apple seed extracts indicated by colorimetry that the intensity of colour was reciprocal to concentration. The antioxidant activity of Red Delicious apple extract was in the order of methanol> chloroform > hexane > aqueous extract. However, the antioxidant activity of Golden Delicious apple extract was in the order of chloroform > hexane > aqueous > methanol extract. Furthermore, the antioxidant activity of Granny Smith apple extract was in the order of chloroform > hexane > methanol > aqueous extract. The findings of this study suggest that all the seed extracts from the three apple varieties could be used as new antioxidant compound options.
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