Comparative Study, Phytochemical Screening and Antioxidant Activities of Three Types of Apple Seed Extracts
http://www.doi.org/10.26538/tjnpr/v6i9.9
Keywords:
Red Delicious, Granny Smith, Golden Delicious, Phytochemical Screening, Antioxidant, Apple SeedAbstract
The “Red Delicious, Golden Delicious, and Granny Smith” apples are widely cultivated and consumed worldwide. Because of their therapeutic phytochemical content, research findings have confirmed their beneficial effects in disease prevention and management. The present study was aimed at determining the phytochemical profile and evaluating the antioxidant activity of the seed extract of three varieties of apple. Water, methanol, chloroform, and hexane were used as solvents to extract the seeds of the three varieties of apples. The three crude apple seed extracts were subjected to preliminary phytochemical screening and antioxidant activity testing
using 2,2-diphenyl-1-picrylhydrazyl (DPPH). The obtained extracts tested positive for alkaloid, flavonoid, saponin, and tannin compounds in phytochemical screening. However, no glycosides or anthraquinones were observed in any of the extracts. The outcome of the antioxidant capacity test for all the varieties of apple seed extracts indicated by colorimetry that the intensity of colour was reciprocal to concentration. The antioxidant activity of Red Delicious apple extract was in the order of methanol> chloroform > hexane > aqueous extract. However, the antioxidant activity of Golden Delicious apple extract was in the order of chloroform > hexane > aqueous > methanol extract. Furthermore, the antioxidant activity of Granny Smith apple extract was in the order of chloroform > hexane > methanol > aqueous extract. The findings of this study suggest that all the seed extracts from the three apple varieties could be used as new antioxidant compound options.
References
Alabri TH, Al Musalami AH, Hossain MA, Weli AM, AlRiyami Q. Comparative study of phytochemical screening, antioxidant and antimicrobial capacities of fresh and dry leaves crude plant extracts of Datura metel L. J King Saud Univ Sci. 2014; 26(3):237-43.
Manandhar NP. Plants and people of Nepal. Timber press; 2002.
Katz L and Baltz RH. Natural product discovery: past, present, and future. J Indust Microb Biotech. 2016; 43(2-3):155-76.
Pisoschi AM and Pop A. The role of antioxidants in the chemistry of oxidative stress: A review. Europ J Med Chem. 2015; 97:55-74.
Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. Oxidative stress: Harms and benefits for human health. Oxid Med Cell Longevity. 2017.
Wu H, Luo T, Li YM, Gao ZP, Zhang KQ, Song JY, Xiao JS, Cao YP. Granny Smith apple procyanidin extract upregulates tight junction protein expression and modulates oxidative stress and inflammation in lipopolysaccharide-induced Caco-2 cells. Food Fun. 2018; 9(6):3321-9.
Li WF, Mao J, Yang SJ, Guo ZG, Ma ZH, Dawuda MM, Zuo CW, Chu MY, Chen BH. Anthocyanin accumulation correlates with hormones in the fruit skin of „Red Delicious‟ and its four generation bud sport mutants. BMC Plant Bio. 2018; 18(1):1-5.
Chinnici F, Bendini A, Gaiani A, Riponi C. Radical scavenging activities of peels and pulps from cv. Golden Delicious apples as related to their phenolic composition. J Agric Food Chem. 2004; 52(15):4684-9.
Bolarinwa IF, Orfila C, Morgan MR. Determination of amygdalin in apple seeds, fresh apples and processed apple juices. Food Chem. 2015; 170:437-42.
Krawitzky M, Arias E, Peiro JM, Negueruela AI, Val J, Oria R. Determination of color, antioxidant activity, and phenolic profile of different fruit tissue of Spanish „Verde Doncella‟apple cultivar. Intern J Food Properties. 2014; 17(10):2298-311.
Oglah MK, Bashir MK, Mustafa YF, Mohammed ET, Khalil RR. Synthesis and biological activities of 3, 5-disubstituted-4- hydroxycinnamic acids linked to a functionalized coumarin. Systematic Rev Pharm. 2020; 11(6):717-25.
Apu AS, Liza MS, Jamaluddin AT, Howlader MA, Saha RK, Rizwan F, Nasrin N. Phytochemical screening and in vitro bioactivities of the extracts of aerial part of Boerhavia diffusa Linn. Asian Pac J Trop Biomed. 2012; 2(9):673-8.
Ikem CJ, Oli AN, Ofori-Attah E, Aning A, Appiah-Opong R, Esimone CO. Screening of Five Herbal Formulations Sold in South-East Nigeria for their Phytochemical Properties, In Vitro Antioxidant, Antiplasmodial and Cytotoxic Activities: Trop J Nat Prod Res. 2022; 6(1):150-5.
Enenebeaku UE, Duru CE, Okotcha EN, Ogidi OI, Mgbemena IC, Nwigwe HC, Enenebeaku CK. Phytochemical Analysis and Antioxidant Evaluation of Crude Extracts from the Roots, Stem and Leaves of Dictyandra arborescens (Welw.): Trop J Nat Prod Res. 2022; 6(1):62-70.
Chivodze RS, Sulemane AI, Magaia I, Saete VN, Rugunate SC, Zimila HE, Munyemana F. Phytochemical screening and Evaluation of Antioxidant and Antimicrobial Activity of Solanum linnaeanum Extracts: Trop J Nat Prod Res. 2022; 6(2):194-201.
Fridayanti A, Purwanto DA, Hendradi E. Preliminary Phytochemical Screening and GC-MS Analysis of Ethanol Extract of Bulbs of Eleutherine sp. Trop J Nat Prod Res. 2022; 6(3):361-4.
Sanusi BS, Idris H, Usman A, Hamza M, Abdullahi B. Phytochemical Profiling, Antimycobacterial Activity, TimeKill Assessment and Mode of Action of the Extract of Musa gracilis Holttum: Trop J Nat Prod Res. 2022; 6(3):376-81.
Owolabi AO, Ndako JA, Owa SO, Oluyori AP, Oludipe EO, Akinsanola BA. Antibacterial and Phytochemical Potentials of Ficus capensis Leaf Extracts Against Some Pathogenic Bacteria: Trop J Nat Prod Res. 2022; 6(3):382-7.
Yupparach P and Konsue A. Phytochemical Screening and Antioxidant Activities of Cornukaempferia chayanii Extracts and in vitro Inhibitory Effects on Digestive Enzymes. Trop J Nat Prod Res. 2022; 6(5):705-8.
Yupparach P, Sumalee A, Konsue A. Phytochemical Screening and Biological Activities of a Remedy from A-thi-sa-ra-wak Scripture as a Folkloric Diabetic Medicine: Trop J Nat Prod Res. 2022; 6(6):863-7.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nat. 1958; 181(4617):1199-200.
Hossain MA and Nagooru MR. Biochemical profiling and total flavonoids contents of leaves crude extract of endemic medicinal plant Corydyline terminalis L. Kunth. Pharmacog J. 2011; 3(24):25-30.
Suresh SN and Nagarajan N. Antimicrobial activity and preliminary phytochemical analysis of Begonia malabarica Lam. J Pure App Microb. 2009; 3(2):801-3.
Gonzalez-Guevara JL, Gonzalez-Lavaut JA, Pino-Rodriguez S, Garcia-Torres M, Carballo-Gonzalez MT, Echemendia-Arana OA, Molina-Torres J, Prieto-Gonzalez S. Phytochemical screening and in vitro antiherpetic activity of four Erythtroxylum species. Acta Farmaceut Bonaer. 2004;
(4):506-9.
Anyasor GN, Ogunwenmo O, Oyelana OA, Akpofunure BE. Phytochemical constituents and antioxidant activities of aqueous and methanol stem extracts of Costus afer Ker Gawl. (Costaceae). Afr J Biotech. 2010; 9(31):4880-4.
Chao PD, Hsiu SL, Hou YC. Flavonoids in herbs: Biological fates and potential interactions with xenobiotics. J Food Drug Anal. 2002; 10(4).
Igbinosa OO, Igbinosa EO, Aiyegoro OA. Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn). Afr J Pharm Pharmacol. 2009; 3(2):58-62.
Thitilertdecha N, Teerawutgulrag A, Rakariyatham N. Antioxidant and antibacterial activities of Nephelium lappaceum L. extracts. LWT-Food Sci Technol. 2008; 41(10):2029-35.
Banso A and Adeyemo S. Phytochemical screening and antimicrobial assessment of Abutilon mauritianum, Bacopa monnifera and Datura stramonium. Biokemistri. 2006; 18(1).
Ayoola GA, Coker HA, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC, Atangbayila TO. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Trop J Pharmac Res. 2008; 7(3):1019-24.
Akharaiyi FC. Antibacterial, phytochemical and antioxidant activities of Datura metel. Int J Pharm Tech Res. 2011; 3(1):478-83.
Vadlapudi V and Kaladhar DS. Antimicrobial study of plant extracts of Datura metel L. against some important disease causing pathogens. Asian Pac J Trop Disease. 2012; 2:S94-7.
Sekar D, Kolanjinathan K, Saranraj P, Gajendiran K. Screening of Phyllanthus amarus, Acalypha indica and Datura metel for its antimicrobial activity against selected pathogens. Int J Pharmaceu Bio Arch. 2012; 3(5):1231-6.
Kara M, Assouguem A, Zerhouni AR, Bahhou J. Phytochemical Content and Antioxidant Activity of Vinegar Prepared from Four Apple Varieties by Different Methods: Trop J Nat Prod Res. 2021; 5(9):1578-85.
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