Evaluation of Ultrasonically Assisted Extraction of the Thai Polyherbal Formulation, Mahajak on Antioxidation and Anti-Inflammation Activities



  • Adisak Thomudtha Ph.D. candidate in Doctor of Philosophy in Pharmacy Program, Faculty of Pharmacy, Mahasarakham University, Maha Sarakham, 44150, Thailand
  • Pornpun Laovachirasuwan Pharmaceutical Chemistry and Natural Product Research Unit, Faculty of Pharmacy, Mahasarakham University, Maha Sarakham, 44150, Thailand
  • Prasob-orn Rinthong Pharmaceutical Chemistry and Natural Product Research Unit, Faculty of Pharmacy, Mahasarakham University, Maha Sarakham, 44150, Thailand


Aromatization, Herbal medicine extraction, Cluster analysis, Principal component analysis, Ultrasonic


Mahajak polyherbal formula is a traditional oil preparation described in the official Thai medicinal textbook. The present study compared biological activities of Mahajak which was prepared by a traditional method and ultrasonically assisted extraction (UAE). The Mahajak samples obtained from four preparative methods including traditional preparation (TP), UAE with sonication time for 10, 20 and 30 minutes (UAE10, UAE20 and UAE30) were determined for in vitro antioxidant and anti-inflammatory activities. GC-MS and principal component analysis were performed to compare their chemical profiles. Results demonstrated the UAE samples did not show difference in the antioxidant activities from that of the TP samples. However, the UAE technique yielded the significance of higher effectiveness on the antiinflammatory activities (p<0.05) as UAE10 and UAE20 showed the most potent antiinflammatory activity (IC50 of 63.12 ± 4.12 and 62.37 ± 2.82 µg/mL, respectively) as compared to TP (IC50 = 74.27 ± 3.54 µg/mL). The GC-MS fingerprints of all UAE samples presented similar GC-MS profiles to that of TP. Ten major chemical components of Mahajak formula were identified and their contents were higher in the UAE samples. In conclusion, the results of this study demonstrated the impact of UAE on Mahajak formula production. The UAE technique improved the percentage yielding and anti-inflammatory activity of Mahajak preparations but not the effect on the chemical components. 


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How to Cite

Thomudtha, A., Laovachirasuwan, P., & Rinthong, P.- orn. (2022). Evaluation of Ultrasonically Assisted Extraction of the Thai Polyherbal Formulation, Mahajak on Antioxidation and Anti-Inflammation Activities: http://www.doi.org/10.26538/tjnpr/v6i11.26. Tropical Journal of Natural Product Research (TJNPR), 6(11), 1900–1905. Retrieved from https://tjnpr.org/index.php/home/article/view/1246