In vitro Antioxidant and Antidiabetic Potentials of Extracts of the Stem Bark of Cylicodiscus gabunensis (Harms) Mimosaceae http://www.doi.org/10.26538/tjnpr/v6i11.19
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Abstract
The study evaluated the antioxidant and antidiabetic activity of extracts of Cylicodiscus gabunensis (CG) by in vitro assay methods. The powdered plant material was successively extracted to yield n-hexane extract (CG-HX), ethyl acetate extract (CG-EA), and methanol extract (CG-ME), respectively. The crude extract (CG-TE) was separately obtained from methanol. Phytochemical screening revealed the presence of alkaloids, flavonoids, phenolic compounds, steroids, and reducing sugars. The total phenolic content (TPC) was highest in the CG-ME (122.559 mg GAE/g extract), followed by CG-TE (121.913 mg GAE/g extract), while the total flavonoids content (TFC) was highest in the CG-EA extract (159.351 mg QE/g). The percentage DPPH free radical scavenging assay shows that the potency of the CG-EA extract (95.4±0.40 %) was comparable to ascorbic acid (96.22±0.692 %), p˂0.05. In the ferric powerreducing antioxidant assay, CG-TE (2.246±0.1796) and CG-ME (2.140±0.1227) exhibited the highest antioxidant activity compared to others. The extracts significantly inhibited α-Amylase and glucose uptake in yeast cells in the in vitro study. This inhibitory potential was concentration-dependent with crude methanol extract (CG-TE) showing comparable inhibitory potency with Acarbose (45.7%) at 0.05 mg/mL but 40.10% at 4.00 g/mL as against 51.27 % for Acarbose. Similarly, CG-TE was more potent than metronidazole, with percentage glucose uptake inhibitory potency of 50.13% and 76.465% at concentrations of 0.05 mg/mL and 4.00 mg/mL, respectively. We conclude that the plant extracts possess significant antidiabetic activity, which correlated with the antioxidant effects and could be further investigated for use in
managing diabetes and related diseases.
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