Effect of Cnidoscolus aconitifolius Extract on Rat Liver Injury Induced with Carbon Tetrachloride (CCl4)



  • Zamharira Muslim Department of Pharmacy, Poltekkes Kemenkes Bengkulu, Bengkulu, Indonesia
  • Jon Farizal Department of Technology Laboratorium Medic, Poltekkes Kemenkes Bengkulu, Bengkulu, Indonesia
  • Rahmad Abdillah Department of Pharmacology, Faculty of Pharmacy, Universitas Andalas, Padang, Indonesia
  • Sunita Sunita Department of Technology Laboratorium Medic, Poltekkes Kemenkes Bengkulu, Bengkulu, Indonesia


Carbon tetrachloride, Liver, Hepatoprotective, Cnidoscolus aconitifolius


Cnidoscolus aconitifolius in Indonesia is consumed as a vegetable which contains phenolic compounds known to have activity in the liver. This study was designed to determine the hepatoprotective effect of various doses of C. aconitifolius extract on the liver of rats injury induced with carbon tetrachloride (CCl4). Thirty-six Wistar rats were used in this study, divided into six groups. Normal group, positive control, negative control, and treatment groups of C. aconitifolius extract (50, 100, and 200 mg/kg). The study analysed serum transaminases and liver histopathology of rats. Serum analysis was performed to determine the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol, and albumin levels. The histopathology of rat liver was analyzed using the Mandja Roenigk method. This study showed that in the groups treated with C. aconitifolius extract at the doses of 50 mg/kg, 100
mg/kg and 200 mg/kg (Group D, E, F) respectively, there was a decrease in ALT, AST and cholesterol levels. In contrast, albumin levels increased compared to the positive control (Group B) (p<0.05). The administration of C. aconitifolius extract (Group F: 200 mg/kg b.w) had almost the same effect as the administration of Curcuma Force® (Group C) (P>0.05). This study showed improvement in the liver function of rats after administration of C. aconitifolius extract. In conclusion, C. aconitifolius possesses a hepatoprotective effect on rat liver damage caused by CCl4 induction.


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How to Cite

Muslim, Z., Farizal, J., Abdillah, R., & Sunita, S. (2022). Effect of Cnidoscolus aconitifolius Extract on Rat Liver Injury Induced with Carbon Tetrachloride (CCl4): http://www.doi.org/10.26538/tjnpr/v6i11.11. Tropical Journal of Natural Product Research (TJNPR), 6(11), 1809–1813. Retrieved from https://tjnpr.org/index.php/home/article/view/1232