Effect of Cnidoscolus aconitifolius Extract on Rat Liver Injury Induced with Carbon Tetrachloride (CCl4) http://www.doi.org/10.26538/tjnpr/v6i11.11

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Zamharira Muslim
Jon Farizal
Rahmad Abdillah
Sunita Sunita

Abstract

Cnidoscolus aconitifolius in Indonesia is consumed as a vegetable which contains phenolic compounds known to have activity in the liver. This study was designed to determine the hepatoprotective effect of various doses of C. aconitifolius extract on the liver of rats injury induced with carbon tetrachloride (CCl4). Thirty-six Wistar rats were used in this study, divided into six groups. Normal group, positive control, negative control, and treatment groups of C. aconitifolius extract (50, 100, and 200 mg/kg). The study analysed serum transaminases and liver histopathology of rats. Serum analysis was performed to determine the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol, and albumin levels. The histopathology of rat liver was analyzed using the Mandja Roenigk method. This study showed that in the groups treated with C. aconitifolius extract at the doses of 50 mg/kg, 100
mg/kg and 200 mg/kg (Group D, E, F) respectively, there was a decrease in ALT, AST and cholesterol levels. In contrast, albumin levels increased compared to the positive control (Group B) (p<0.05). The administration of C. aconitifolius extract (Group F: 200 mg/kg b.w) had almost the same effect as the administration of Curcuma Force® (Group C) (P>0.05). This study showed improvement in the liver function of rats after administration of C. aconitifolius extract. In conclusion, C. aconitifolius possesses a hepatoprotective effect on rat liver damage caused by CCl4 induction.

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How to Cite
Muslim, Z., Farizal, J., Abdillah, R., & Sunita, S. (2022). Effect of Cnidoscolus aconitifolius Extract on Rat Liver Injury Induced with Carbon Tetrachloride (CCl4): http://www.doi.org/10.26538/tjnpr/v6i11.11. Tropical Journal of Natural Product Research (TJNPR), 6(11), 1809–1813. Retrieved from https://tjnpr.org/index.php/home/article/view/1232
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References

Singh A, Joshi S, Joshi A, Patni P, Tewari D. Chapter 8 - Druginduced hepatotoxicity. In: Alavian SM, Nabavi SM, Nabavi SF, Silva Bioactive Compounds, and Plant Extracts in Liver Diseases ASBTI of N, eds. Influence of Nutrients, Bioactive Compounds, and Plant Extracts in Liver Diseases. Academic Press; 2021; 141- 157.

Saha P, Talukdar AD, Nath R, Sarker SD, Nahar L, Sahu J and Choudhury MD. Role of natural phenolics in hepatoprotection: A mechanistic review and analysis of regulatory network of associated genes. Front Pharmacol. 2019; 10(MAY):1-25.

Awoyinka OA, Balogun IO, Ogunnowo AA. Phytochemical screening and in vitro bioactivity of Cnidoscolus aconitifolius (Euphorbiaceae). J Med Plants Res. 2007; 1(3):63-65.

Figueroa-Valverde L, Díaz-Cedillo F, Camacho-Luis A, Ramos ML. Efectos inducidos por Ruta graveolens L., Cnidoscolus chayamansa McVaugh y Citrus aurantium L. sobre los niveles de glucosa, colesterol y triacilglicéridos en un modelo de rata diabética. Rev Bras Farmacogn. 2009; 19(4):898-907.

Miranda-Velasquez L, Oranday-Cardenas A, Lozano-Garza H, Rivas-Morales C, Chamorro-Cevallos G, Cruz-Vega DE. Hypocholesterolemic Activity from the Leaf Extracts of Cnidoscolus chayamansa. Plant Foods Hum Nutr. 2010; 65(4):392-395.

Onasanwo SA, Oyagbemi AA, Saba AB. Anti-inflammatory and analgesic properties of the ethanolic extract of Cnidoscolus aconitifolius in rats and mice. J Basic Clin Physiol Pharmacol. 2011;22(1-2):34-41.

Oyagbemi AA and Odetola AA. Hepatoprotective effects of ethanolic extract of Cnidoscolus aconitifolius on paracetamolinduced hepatic damage in rats. Pakistan J Biol Sci. 2010; 13(4):164-169.

Oyagbemi AA and Odetola AA. Hepatoprotective and nephroprotective effects of Cnidoscolus aconitifolius in protein energy malnutrition induced liver and kidney damage. Pharmacognosy Res. 2013;5(4):260-264.

Ross-ibarra J and Molina-cruz A. The ethnobotany of chaya (Cnidoscolus aconitifolius ssp. aconitifolius breckon): Econ Bot. 2002; 56(4):350-365.

Somade OT, Ugbaja RN, Idowu MA, Akinloye OA. Cnidoscolus aconitifolius leaf extract and ascorbate confer amelioration and protection against dimethyl nitrosamine-induced renal toxicity and testicular abnormalities in rats. Toxicol Reports. 2021; 8(April):1098-1108.

Obichi E, Monago C, Belonwu D. Effect of Cnidoscolus aconitifolius (Family Euphorbiaceae) Aqueous Leaf Extract on Some Antioxidant Enzymes and Haematological Parameters of High Fat Diet and Streptozotocin Induced Diabetic Wistar Albino Rats. J Appl Sci Environ Manag. 2015; 19(2):201.

Ali SA, Sharief NH, Mohamed YS. Hepatoprotective Activity of Some Medicinal Plants in Sudan. Evidence-based Complement Altern Med. Published online 2019.

Direktur Jenderal Bina Kefarmasian dan Alat Kesehatan. Farmakope Herbal Indonesia. II.; 2017.

Madrigal-Santillán, E., Madrigal-Bujaidar, E., Álvarez-González, I., Sumaya-Martínez, M. T., Gutiérrez-Salinas, J., Bautista, M., Morales-González, Á., García-Luna y González-Rubio, M., Aguilar-Faisal, J. L., & Morales-González, J. A.. Review of natural products with hepatoprotective effects. World J Gastroenterol. 2014; 20(40):14787-14804.

Chattopadhyay RR. Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract: Part II. J Ethnopharmacol. 2003; 89:217-219.

Weber LWD, Boll M, Stampfl A. Hepatotoxicity and mechanism of action of haloalkanes: Carbon tetrachloride as a toxicological model. Crit Rev Toxicol. 2003;33(2):105-136.

Slater TF. Free-radical mechanisms in tissue injury. Biochem J. 1984; 222(1):1-15.

Natsume M, Tsuji H, Harada A, Akiyama M, Yano T, Ishikura H, Nakanishi I, Matsushima K, Kaneko S, Mukaida N. Attenuated liver fibrosis and depressed serum albumin levels in carbon tetrachloride-treated IL-6-deficient mice. J Leukoc Biol. 1999; 66(4):601-608.

Boll M, Weber LWD, Becker E, Stampfl A. Pathogenesis of Carbon Tetrachloride-Induced Hepatocyte Injury Bioactivation of CC14 by Cytochrome P450 and Effects on Lipid Homeostasis. Zeitschrift fur Naturforsch - Sect C J Biosci. 2001; 56(1-2):111- 121.

Farkhondeh T and Samarghandian S. The hepatoprotective effects of curcumin against drugs and toxic agents: an updated review. Toxin Rev. 2016; 35(3-4):133-140.

Bhardwaj RK, Glaeser H, Becquemont L, Klotz U, Gupta SK, Fromm MF. Piperine, a major constituent of black pepper, inhibits human P-glycoprotein and CYP3A4. J Pharmacol Exp Ther. 2002; 302(2):645-650.

Tsukamoto S, Cha BC, Ohta T. Dipiperamides A, B, and C: Bisalkaloids from the white pepper Piper nigrum inhibiting CYP3A4 activity. Tetrahedron. 2002;58(9):1667-1671.

Maulina M. Zat-Zat Yang Mempengaruhi Histopatologi Hepar. Unimal Press; 2018.

Soni KB and Kuttan R. Effect of oral curcumin administration on serum peroxides and cholesterol levels in human volunteers. Indian J Physiol Pharmacol. 1992; 36(4):273-275.

Ramos-Gomez M, Figueroa-Pérez MG, Guzman-Maldonado H, Loarca-Piña G, Mendoza S, Quezada-Tristán T and ReynosoCamacho R. Phytochemical Profile, Antioxidant Properties and Hypoglycemic Effect of Chaya (Cnidoscolus Chayamansa) in STZ-Induced Diabetic Rats. J Food Biochem. 2017; 41(1):1-9.

Kuti JO and Konuru HB. Antioxidant Capacity and Phenolic Content in Leaf Extracts of Tree Spinach (Cnidoscolus spp.). J Agric Food Chem. 2004; 52(1):117-121.

Numa S, Rodríguez L, Rodríguez D, Coy-Barrera E. Susceptibility of Tetranychus urticae Koch to an ethanol extract of Cnidoscolus aconitifolius leaves under laboratory conditions. Springerplus. 2015; 4(1).

Sarmiento-Franco L, Sandoval-Castro CA, McNab JM, QuijanoCervera R, Reyes-Ramirez RR. Effect of age of regrowth on chemical composition of chaya (Cnidoscolus aconitifolius) leaves. J Sci Food Agric. 2003; 83(6):609-612.

Loarca-Piña G, Mendoza S, Ramos-Gómez M, Reynoso R. Antioxidant, Antimutagenic, and Antidiabetic Activities of Edible Leaves from Cnidoscolus chayamansa Mc. Vaugh. J Food Sci. 2010; 75(2):68-72.

Valenzuela Soto, Ramón, Morales Rubio, María Eufemia, Verde Star, María Julia, Oranday Cárdenas, Azucena, Preciado-Rangel, Pablo, Antonio González, Jacob, & Esparza-Rivera, Juan Ramón. Cnidoscolus chayamansa hidropónica orgánica y su capacidad hipoglucemiante, calidad nutraceutica y toxicidad. Rev Mex Ciencias Agrícolas. 2017; 6(4):815.

Adewusi EA and Afolayan AJ. A review of natural products with hepatoprotective activity. J Med Plants Res. 2010; 4(13):1318- 1334.

Husna F, Arozal W, Yusuf H, Safarianti S. Protective effect of mangiferin on doxorubicin-induced liver damage in animal model. J Res Pharm. 2022; 26(1):44-51.

Zaĉıragıć Asija, Višnja Muzıka, Amina Valjevac, Amela Dervıševıć, Maja Mıtrašınovıć-Brulıć, Muhamed Foĉak, Esad Ćosovıć, Selma Alıĉelebıć, Samra Ĉustovıć, And Damir Suljevıć.2019. The protective effects of vitamin D3 on histopathology of pancreas and liver in streptozotocin-induced diabetic rats. J Res Pharm. 2022; 26(2):325-333.

Yagmurca M, Bas O, Mollaoglu H, Sahin O, Nacar A, Karaman O, Songur A. Protective Effects of Erdosteine on Doxorubicininduced Hepatotoxicity in Rats. Arch Med Res. 2007; 38(4):380- 385.

Ross Michael H, Kaye Gordon I, Wojciech P. Histology a text and atlas. Published online 2003.

Zheng YP, Zhong XY, Huang YS, Zheng C Bin. HCBP6 is involved in the development of hepatic steatosis induced by highfat diet and CCl4 in rats. Ann Hepatol. 2018; 17(3):511-518.