The Inhibitory Effect of Common Edible Mushrooms on Hepatic Cancer Cell Invasion

http://www.doi.org/10.26538/tjnpr/v6i10.14

Authors

  • Nguyen H.N. Minh NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam
  • Vo T.N. My Department of Pharmacy, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam
  • Vo T. Dat Chemical Engineering, Ho Chi Minh City University of Technology, Ho Chi Minh City, Vietnam
  • Dai-Hung Ngo Institute of Applied Technology, Thu Dau Mot University, Binh Duong Province, Vietnam
  • Thanh S. Vo Institute of Applied Technology and Sustainable Development, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam

Keywords:

Hep-3B, Anticancer,, Metastasis,, MMPs,, TIMPs.

Abstract

Prevention of liver cancer cell metastasis significantly contributes to the lowering of mortality incidence. Moreover, a daily diet rich in natural ingredients has been reported to play important roles in prevention of metastatic development. This study, therefore, focused to screen and identify the potential edible mushrooms that possess the inhibitory effect on the invasion of hepatic cancer cells. The edible mushrooms were extracted by distilled water under temperature of 100oC for 3 h. The wound healing assay was used to investigate the cancer cell migration. The cytotoxicity of extracts (100-1000 µg/ml) on Hep-3B cells was examined by MTT assay. The expression levels of genes controlling migration process was assessed by real time PCR. Among different edible mushrooms, the extracts of Lentinula edodes (LE) and Volvariella volvacea (VV) have been found to be potential agents for inhibition of Hep-3B cell invasion. This inhibition was identified due to induction of apoptosis and regulation of genes involved in migration process. In particular, the treatment of L. edodes and V. volvacea extracts increased the expression of apoptosis-activating molecules, including caspase-3, -8, -9, and Bax. Moreover, these extracts caused suppression of enzyme matrix metalloproteinases, including MMP-2, MMP-9, and MMP-13, and up-regulation of tissue inhibitors of metalloproteinases, including TIMP-1 and TIMP-2. Notably, the activities of L. edodes and V. volvacea extracts were not due to cytotoxic effect. These results indicated that LE and VV extracts possess the promising anti-metastatic activity, supporting them as potential functional foods for prevention of metastasis of hepatic cancer cells.

References

Steeg PS. Tumor metastasis: mechanistic insights and clinical challenges. Nat Med. 2006; 12: 895-904.

Welch DR and Hurst DR. Defining the Hallmarks of Metastasis. Cancer Res. 2019; 79:3011-3027.

Tsilimigras DI, Brodt P, Clavien PA, Muschel RJ, D’Angelica MI, Endo I, Parks RW, Doyle M, de Santibañes E, Pawlik TM. Liver metastases. Nat Rev Dis Primers. 2021; 7:1-23.

Te Boekhorst V and Friedl P. Plasticity of Cancer Cell Invasion- Mechanisms and Implications for Therapy. Adv Cancer Res. 2016; 132:209-264.

Debela DT, Muzazu SG, Heraro KD, Ndalama MT, Mesele BW, Haile DC, Kitui SK, Manyazewal T. New approaches and procedures for cancer treatment: Current perspectives. SAGE Open Med. 2021; 9:1-10.

Ahmad R, Khan MA, Srivastava AN, Gupta A, Srivastava A, Jafri TR, Siddiqui Z, Chaubey S, Khan T, Srivastava AK. Anticancer Potential of Dietary Natural Products: A Comprehensive Review. Antican Agents Med Chem. 2020; 20:122-236.

Assemie A and Abaya G. The Effect of Edible Mushroom on Health and Their Biochemistry. Int J Microbiol. 2022; 2022:1-7.

Patel S and Goyal A. Recent developments in mushrooms as anti- cancer therapeutics: a review. 3 Biotech. 2012; 2:1-15.

Valverde ME, Hernández-Pérez T, Paredes-López O. Edible mushrooms: improving human health and promoting quality life. Int J Microbiol. 2015; 2015:1-14.

Panda SK, Sahoo G, Swain SS, Luyten W. Anticancer Activities of Mushrooms: A Neglected Source for Drug Discovery. Pharmaceut (Basel). 2022; 15:1-25.

Jonkman JE, Cathcart JA, Xu F, Bartolini ME, Amon JE, Stevens KM, Colarusso P. An introduction to the wound healing assay using live-cell microscopy. Cell Adh Migr. 2014; 8:440-451.

Rollando R, Monica E, Aftoni M. In vitro Cytotoxic Potential of Sterculia quadrifida Leaf Extract Against Human Breast Cancer Cell Lines. J Trop J Nat Prod Res. 2022; 6:1228-1232.

Livak KJ and Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001; 25:402-408.

Martin TA and Jiang WG. Loss of tight junction barrier function and its role in cancer metastasis. Biochim Biophys Acta. 2009; 1788:872-891.

Sheng SQ, Yu LY, Zhou XW, Pan HY, Hu FY, Liu JL. Paeonol prevents migration and invasion, and promotes apoptosis of cervical cancer cells by inhibiting 5‑lipoxygenase. Mol Med Rep. 2021; 23:1-8.

Liang ZE, Yi YJ, Guo YT, Wang RC, Hu QL, Xiong XY. Inhibition of migration and induction of apoptosis in LoVo human colon cancer cells by polysaccharides from Ganoderma lucidum. Mol Med Rep. 2015; 12:7629-7636.

Shi X, Luo X, Chen T, Guo W, Liang C, Tang S, Mo J. Naringenin inhibits migration, invasion, induces apoptosis in human lung cancer cells and arrests tumour progression in vitro. J Cell Mol Med. 2021; 25:2563-2571.

Li Y, Sun W, Han N, Zou Y, Yin D. Curcumin inhibits proliferation, migration, invasion and promotes apoptosis of retinoblastoma cell lines through modulation of miR-99a and JAK/STAT pathway. BMC Cancer. 2018; 18:1-9.

Sánchez-Martín V, Jiménez-García L, Herranz S, Luque A, Acebo P, Amesty Á, Estévez-Braun A, de Las Heras B, Hortelano S. α- Hispanolol Induces Apoptosis and Suppresses Migration and Invasion of Glioblastoma Cells Likely via Downregulation of MMP-2/9 Expression and p38MAPK Attenuation. Front Pharmacol. 2019; 10:1-16.

Webb AH, Gao BT, Goldsmith ZK, Irvine AS, Saleh N, Lee RP, Lendermon JB, Bheemreddy R, Zhang Q, Brennan RC, Johnson D, Steinle JJ, Wilson MW, Morales-Tirado VM. Inhibition of MMP-2 and MMP-9 decreases cellular migration, and angiogenesis in in vitro models of retinoblastoma. BMC Cancer. 2017; 17:1-11.

Hong O-Y, Jang H-Y, Lee Y-R, Jung SH, Youn HJ, Kim J-S. Inhibition of cell invasion and migration by targeting matrix metalloproteinase-9 expression via sirtuin 6 silencing in human breast cancer cells. Sci Report. 2022; 12:12125.

Mazloum-Ardakani M, Barazesh B, Moshtaghioun SM, Sheikhha MH. Designing and optimization of an electrochemical substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay. Sci Rep. 2019; 9:1-8.

Pfeffer CM and Singh ATK. Apoptosis: A Target for Anticancer Therapy. Int J Mol Sci. 2018; 19:1-10.

Su Z, Yang Z, Xu Y, Chen Y, Yu Q. Apoptosis, autophagy, necroptosis, and cancer metastasis. Mol Cancer. 2015; 14:1-14.

McIlwain DR, Berger T, Mak TW. Caspase functions in cell death and disease. Cold Spring Harb Perspect Biol. 2013; 5:1-28.

Artanti AN, Pratiwi LD, Rakhmawati R, Prihapsara F. Cytotoxic effects of parijoto (Medinilla speciosa) methanol extract on mcf- 7/her-2 cells through increased of caspase-9 protein and decreased expression of vascular endothelial growth factor. EurAsian J BioSci. 2020; 14:7861-7868.

Rajabi S, Maresca M, Yumashev AV, Choopani R, Hajimehdipoor H. The Most Competent Plant-Derived Natural Products for Targeting Apoptosis in Cancer Therapy. Biomole. 2021; 11:1-29.

Stamenkovic I. Matrix metalloproteinases in tumor invasion and metastasis. Semin Cancer Biol. 2000; 10:415-433.

Gonzalez-Avila G, Sommer B, Mendoza-Posada DA, Ramos C, Garcia-Hernandez AA, Falfan-Valencia R. Matrix metalloproteinases participation in the metastatic process and their diagnostic and therapeutic applications in cancer. Crit Rev Oncol Hematol. 2019; 137:57-83.

Lv Y, Zhao X, Zhu L, Li S, Xiao Q, He W, Yin L. Targeting intracellular MMPs efficiently inhibits tumor metastasis and angiogenesis. Theranostics. 2018; 8:2830-2845.

Winer A, Adams S, Mignatti P. Matrix Metalloproteinase Inhibitors in Cancer Therapy: Turning Past Failures Into Future Successes. Mol Cancer Ther. 2018; 17:1147-1155.

Murphy G. Tissue inhibitors of metalloproteinases. Genome Biol. 2011; 12:1-7.

Kaleta B, Górski A, Zagożdżon R, Cieślak M, Kaźmierczak- Barańska J, Nawrot B, Klimaszewska M, Malinowska E, Górska S, Turło J. Selenium-containing polysaccharides from Lentinula edodes—Biological activity. Carbohydrate Polymers. 2019; 223:1-8.

Sangthong S, Pintathong P, Pongsua P, Jirarat A, Chaiwut P. Polysaccharides from Volvariella volvacea Mushroom: Extraction, Biological Activities and Cosmetic Efficacy. J Fungi (Basel). 2022; 8:1-19.

Kothari D, Patel S, Kim SK. Anticancer and other therapeutic relevance of mushroom polysaccharides: A holistic appraisal. Biomed Pharmacother. 2018; 105:377-394.

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Published

2022-10-01

How to Cite

H.N. Minh, N., T.N. My, V., T. Dat, V., Ngo, D.-H., & S. Vo, T. (2022). The Inhibitory Effect of Common Edible Mushrooms on Hepatic Cancer Cell Invasion: http://www.doi.org/10.26538/tjnpr/v6i10.14. Tropical Journal of Natural Product Research (TJNPR), 6(10), 1644–1649. Retrieved from https://tjnpr.org/index.php/home/article/view/1217

Issue

Vol. 6 No. 10 (2022): Tropical Journal of Natural Product Research

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