The Inhibitory Effect of Common Edible Mushrooms on Hepatic Cancer Cell Invasion


  • Nguyen H.N. Minh NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam
  • Vo T.N. My Department of Pharmacy, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam
  • Vo T. Dat Chemical Engineering, Ho Chi Minh City University of Technology, Ho Chi Minh City, Vietnam
  • Dai-Hung Ngo Institute of Applied Technology, Thu Dau Mot University, Binh Duong Province, Vietnam
  • Thanh S. Vo Institute of Applied Technology and Sustainable Development, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam


Hep-3B, Anticancer,, Metastasis,, MMPs,, TIMPs.


Prevention of liver cancer cell metastasis significantly contributes to the lowering of mortality incidence. Moreover, a daily diet rich in natural ingredients has been reported to play important roles in prevention of metastatic development. This study, therefore, focused to screen and identify the potential edible mushrooms that possess the inhibitory effect on the invasion of hepatic cancer cells. The edible mushrooms were extracted by distilled water under temperature of 100oC for 3 h. The wound healing assay was used to investigate the cancer cell migration. The cytotoxicity of extracts (100-1000 µg/ml) on Hep-3B cells was examined by MTT assay. The expression levels of genes controlling migration process was assessed by real time PCR. Among different edible mushrooms, the extracts of Lentinula edodes (LE) and Volvariella volvacea (VV) have been found to be potential agents for inhibition of Hep-3B cell invasion. This inhibition was identified due to induction of apoptosis and regulation of genes involved in migration process. In particular, the treatment of L. edodes and V. volvacea extracts increased the expression of apoptosis-activating molecules, including caspase-3, -8, -9, and Bax. Moreover, these extracts caused suppression of enzyme matrix metalloproteinases, including MMP-2, MMP-9, and MMP-13, and up-regulation of tissue inhibitors of metalloproteinases, including TIMP-1 and TIMP-2. Notably, the activities of L. edodes and V. volvacea extracts were not due to cytotoxic effect. These results indicated that LE and VV extracts possess the promising anti-metastatic activity, supporting them as potential functional foods for prevention of metastasis of hepatic cancer cells.


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How to Cite

H.N. Minh, N., T.N. My, V., T. Dat, V., Ngo, D.-H., & S. Vo, T. (2022). The Inhibitory Effect of Common Edible Mushrooms on Hepatic Cancer Cell Invasion: Tropical Journal of Natural Product Research (TJNPR), 6(10), 1644–1649. Retrieved from