GC-MS Analysis and Antioxidant Potential of the Stem and Leaf Extracts of Dendrophthoe pentandra (L.) Miq. Hemiparasite on Shorea roxburghii G. Don and Cassia fistula L.

http://www.doi.org/10.26538/tjnpr/v6i10.13

Authors

  • Pasakorn Bunchalee Department of Biology, Faculty of Science, Mahasarakham University, 44150, Thailand
  • Orawan Monthakantirat Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, 4002, Thailand
  • Prathan Luecha Department of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, 4002, Thailand
  • Tatiya Srichai Department of Biology, Faculty of Science, Mahasarakham University, 44150, Thailand
  • Suthira Maneechai Department of Biology, Faculty of Science, Mahasarakham University, 44150, Thailand

Keywords:

Antioxidant,, Dendrophthoe pentandra,, Hemiparasite,, Phytochemical profiles

Abstract

Dendrophthoe pentandra (L.) Miq. (Kafak mamung) is a hemiparasitic plant utilized for several ethnopharmacological and medicinal purposes. However, the hemiparasitic plant vary in correlation with the species from host plant, due to variation in the compound’s profiles. This study was aimed to determine the compounds contained in leaf and stem extracts from various host plants. Soxhlet extraction was used to prepare crude extracts using ethanol and ethyl acetate as solvents. Phytochemical profiles of the stem and leaf were analyzed by GC-MS to determine the total phenolic content (TPC) and total flavonoid content (TFC), while antioxidant activities were examined using DPPH and ABTS assays. Folin-Ciocalteu colorimetry showed that ethanol extract of D. pentandra hemiparasite stem growing on Shorea roxburghii had the highest TPC (398.45± 2.49 mg GAE/g extract) and DPPH assays 88.518± 0.339%. Chemical analysis using GC-MS revealed the presence of 14 compounds including 9-octadecanamide (Z)-, hexadecanamide and octadecanamide. The phytochemical profiles of the compounds identified in the extracts of D. pentandra showed that this plant has a potential for future health promotion.

References

Těšitel J, Plavcová L, Cameron DD. Interactions between hemiparasitic plants and their hosts: The importance of organic carbon transfer. Plant Signal Behav. 2010; 5(9):1072-1076.

Artanti N, Firmansyah T, Darmawan A. Bioactivities Evaluation of Indonesian Mistletoes (Dendrophthoe pentandra (L.) Miq. Leaves Extracts. J Appl Pharm Sci. 2012; 2(1):24-27.

Yee LS, Fauzi NFM, Najihah NN, Daud NM, Sulain MD. Study of Dendrophthoe Pentandra Ethyl Acetate Extract as Potential Anticancer Candidate on Safety and Toxicity Aspects. J Anal Pharm Res. 2017; 6(1):1-11.

O’Neill AR and Rana SK. An ethnobotanical analysis of parasitic plants (Parijibi) in the Nepal Himalaya. J Ethnobio Ethnomedicine. 2016; 12(14):1-15.

Elsyana V, Bintang M, Priosoeryanto BP. Cytotoxicity and antiproliferative activity assay of clove mistletoe (Dendrophthoe pentandra (L.) Miq.) leaves extracts. Advances in Pharmacol and Pharmaceut Sci. 2016; (2016):3242698.

Mustarichie R, Warya S, Saptarini NM, Ramdhani D. Total Flavonoid Content and Anti-Inflammatory Properties of Indonesian Mistletoes (Dendrophthoe pentandra L. (Miq.)) Ethanol Extract. World J Pharmaceut Res WJPR. 2015; 4(4):287-302.

Inta A, Shengji P, Balslevc H, Wangpakapattanawonga P, Trisonthia, C. A comparative study on medicinal plants used in Akha's traditional medicine in China and Thailand, cultural coherence or ecological divergence? J Ethnopharmacol. 2008; 116(3):508-517.

Sahakitpichan P, Disadee W, Buntawong R, Chimnoi N, Ruchirawat S, kanchanapoom T. A furan-2-carbonyl Cglucoside and an alkyl glucoside from the parasitic plant, Dendrophthoe pentandra. Phytochem Lett. 2017; 21:90-93.

Hardiyanti R, Marpaung L, Adnyana IK, Simanjuntak P. Isolation of Quercitrin from Dendrophthoe pentandra leaves and it’s antioxidant and antibacterial activities. Rasayan J. Chem. 2019; 12(4):1822-1827.

Artanti N, Ma’arifa Y, Hanafi M. Isolation and identification of active antioxidant compound from star fruit (Averrhoa carambola) mistletoe (Dendrophthoe pentandra L.) Miq. Ethanol extract. J Appl Sci. 2006; 6:1659-63.

Amin I, Norazaidah Y, Hainida KIE. Antioxidant activity and phenolic content of raw and blanched Amaranthus species. Food Chem. 2006; 94(1):47-52.

Pothitirat W, Chomnawang MT, Supabphol R, Gritsanapan W. Comparison of bioactive compounds content, free radical scavenging and anti-acne inducing bacteria activities of extracts from the mangosteen fruit rind at two stages of maturity. Fitoterapia. 2009; 80(7):442-7.

Likhitwitayawuid K, Sornsute A, Sritularak B, Ploypradith P. Chemical transformations of oxyresveratrol (trans-2,4,3’,5’- tetrahydroxystilbene) into a potent tyrosinase inhibitor and a strong cytotoxic agent. Bioorg Med Chem Lett. 2006; 16(21):5650-3.

Payet B, Shum Cheong Sing ASC, Smadja J. Assessment of antioxidant activity of cane brown sugars by ABTS and DPPH radical scavenging assays: determination of their polyphenolic and volatile constituents. J Agric Food Chem. 2005; 53(26):10074-9.

Suphrom N, Sonyota W, Insumronga K, Sawangsupa P, Sutamuanga P, Ingkaninanb K. GC-MS analysis and in vitro anti-androgenic activity of Kaempferia rotunda Linn extract. NUJST. 2017; 4(25):34-43.

Osman MA, Mahmoud GI, Shoman SS. Correlation Between Total Phenols Content, Antioxidant Power and Cytotoxicity. Biointerface Res in Appl Chem. 2021; 11(3):10640–10653.

Hadi MY, Mohammed GJ, Hameed IH. Analysis of bioactive chemical compounds of Nigella sativa using gas chromatography-mass spectrometry. J Pharmacogn Phytotherapy. 2016; 8(2):8-24.

Bharose A and Gajera H. Antifungal Activity and Metabolites Study of Bacillus Strain Against Aflatoxin Producing Aspergillus. J Appl Microbiol Biochem. 2018; (2):1-8.

Kumaresan S, Senthilkumar V, Stephen A, Balakumar BS. GC- MS analysis and pass-assisted prediction of biological activity spectra of extract of Phomopsis sp. Isolated from Andrographis paniculata. World J Pharm Res. 2015; 4(1):1035-53.

Jegajeevanram P, Alhaji NMI, Velavan S. Identification of insecticidal components of Mango ginger rhizome and Tagetes erecta flower extracts by GC-MS analysis. IJCPA. 2014; 1(4):203-7.

Aman VB, Samuel LA, Saradhi MP, Rao NB, Vamsikrishna AN, Sudhakar M, Radhakrishnan TM. Antibacterial, antioxidant activity and GC-MS analysis of Eupatorium odoratum. Asian J Pharm Clin Res. 2012; 5(2):99-106.

Idan SA, Al-Marzoqi AH, Hameed IH. Spectral analysis and anti-bacterial activity of methanolic fruit extract fruit extract of Citrullus colocynthis using gas chromatography-mass spectrometry. Afr J Biotechnol. 2015; 14(46):3131-58.

Islam MT, Ali ES, Uddin S.J, Shaw S, Islam MA, Ahmed MI, Chandra SM, Karmakar UK, Yarla NS, Khan IN, Billah MM, Pieczynska MD, Zengin G, Malainer C, Nicoletti F, Gulei D, Berindan-Neagoe I, Apostolov A, Banach M, Yeung AWK, El- Demerdash A, Xiao J, Dey P, Yele S, Jóźwik A, Strzałkowska N, Marchewka J, Rengasamy KRR, Horbańczuk J, Kamal MA, Mubarak MS, Mishra SK, Shilpi JA, Atanasov AG. Phytol: A review of biomedical activities. Food Chem Toxicol. 2018; 121: 82-94.

Senbagalakshmi P, Muthukrishnan S, Jebasingh T, Kumar S, Rao MV, Senbagalakshmi P. Squalene, Biosynthesis and its role in production of bioactive compounds, a Proper Scientific Challenge-A Review. J Emerg Tech and Innov Res. 2019; 6(2):505-526.

Naumoska K, Jug U, Metličar V, Vovk I. Oleamide, a Bioactive Compound, Unwittingly Introduced into the Human Body through Some Plastic Food/Beverages and Medicine Containers. Foods. 2020; 9(5):549.

Alharits L, Handayani W, Yasman, Hemelda NM. Phytochemical Analysis and Antioxidant activity of Leaves and Flowers Extracts of Mistletoe (Dendrophthoe pentandra (L.) Miq.), Collected from UI Campus, Depok. AIP Conference Proceedings. 2019; 2168(1):020101-1-8.

Yismairai E, Hemelda NM, Yasman HW. Antioxidant activity of extract of Mistletoe, Dendrophthoe pentandra (L.) Miq., lived in three different host plants, collected from Kampus UI, Depok. AIP Conference Proceed. 2019; 2168(1):020100-1-7.

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Published

2022-10-01

How to Cite

Bunchalee, P., Monthakantirat, O., Luecha, P., Srichai, T., & Maneechai, S. (2022). GC-MS Analysis and Antioxidant Potential of the Stem and Leaf Extracts of Dendrophthoe pentandra (L.) Miq. Hemiparasite on Shorea roxburghii G. Don and Cassia fistula L.: http://www.doi.org/10.26538/tjnpr/v6i10.13. Tropical Journal of Natural Product Research (TJNPR), 6(10), 1638–1643. Retrieved from https://tjnpr.org/index.php/home/article/view/1216