Evaluation of the Subacute Toxic Effects of the Alkaloids of the Seeds of Peganum harmala L in the Liver, Kidney and Ovary of Female Rats

http://www.doi.org/10.26538/tjnpr/v6i10.12

Authors

  • Mahdeb Nadia Department of Biochemistry – FSNV – Ferhat Abbas Sétif1 University
  • Allouni Rima Department of Biochemistry – FSNV – Ferhat Abbas Sétif1 University
  • Reguai Rima Department of Biochemistry – FSNV – Ferhat Abbas Sétif1 University
  • Azzoug khouloud Department of Biochemistry – FSNV – Ferhat Abbas Sétif1 University
  • Bouzidi Abdelouahab Department of Biochemistry – FSNV – Ferhat Abbas Sétif1 University

Keywords:

Peganum harmala,, Alkaloids,, Toxicity,, Rat,, Organ.

Abstract

Peganum harmala (Harmel) is a medicinal plant used in traditional Algerian medicine to treat several diseases. The subacute total alkaloid toxicity of Peganum harmala seeds on the major organs of female Wistar rats treated with 5.5 mg/kg, i.p. (1/30 LD50) for 30 days was assessed in this study. The treated animals revealed a significant increase in body weight after the first 10 days of treatment followed by a significant drop after the 20th day. The analysis of the blood and serum parameters showed: at p <0.05, a significant elevation in relative liver mass was recorded in parallel with the significant increase in glutamate-pyruvate transaminase (TGP) in liver parameters while hematologic and renal parameters did not change significantly, the exception however was for Mean Platelet Volume (MPV), Platelet Distribution Width (PDW), and urea. Histological sections of the liver of the treated rats showed some necrosis, a slight dilation of the Centro-lobular vein, mobilization of Kupffer cells, fatty deposits, blood (abnormal accumulation of blood) and hepatocyte ballooning. Some renal histological sections revealed glomerular atrophy. Observation of histological sections of the ovaries showed abnormalities: follicles developed devoid of oocytes, and a very limited number of developed follicles. In conclusion, the alkaloids of Peganum harmala have a toxic effect mainly on the ovaries, kidney and liver of female Wistar rats.

References

Nadège DL. Study of medicinal plants in the Maghreb: traditional uses and phytochemical studies. Thesis, Toulouse: University Paul Sabatier, France; 2017.

Merck Index. An Encyclopedia of chemicals, drugs and biologicals. (11th Ed.). New York: S. Budavari. Rahway; 1989.

Calderoni M, Altare M, Mastracci L, Grillo F, Cornara L, Pagano A. Potential Risks of Plant Constituents in Dietary Supplements: Qualitative and Quantitative Analysis of Peganum harmala. Molecules. 2021; 26(5):1368.

Guergour H. Study of the morphological, phytochemical and pharmacotoxicological aspects of the Peganum harmala plant. Thesis, Setif: University Ferhat Abbas Sétif1, Algeria, 2018.

Merad R. Contribution to the knowledge of the Algerian pharmacopoeia. Thesis, Algiers: University of Algiers, Algeria, 1973.

Djafer R, Akil DSR, Boukachabia R, Megueddem M, Djafer R, Boukachabia R, Megueddem M. About a case of lethal harmel poisoning (Peganum harmala L.). Phytothérapie. 2017; 15:288- 289.

Hammiche V, Merad R, Azzouz M. Toxic plants for medicinal use around the Mediterranean. (1st ed.). Paris: Springer; 2013.

Mahmoudian M, Jalilpour H, Salehian P. Toxicity of Peganum harmala: Review and a Case Report Iran. J Pharmacol Ther. 2002; 1(1):1-4.

Achour S, Rhalem N, Khattabi A, Lofti H, Mokhtari A, Soulaymani A, Turcant, A, Soulaymani Bencheikh R. Peganum harmala L. poisoning in Morocco: about 200 cases. Therapies. 2012; 67(1):53–58.

Mahdeb N, Bettihi S, Ghadjati N, Bouzidi A. Evaluation of Acute Toxicity of the Alkaloids of Peganum harmala L Seeds in Albino Wistar Female Rats. Adv Pharmacol Pharm. 2020; 8(2):24-30.

Bouzidi A, Mahdeb N, Kara N. Toxicity studies of alkaloids of seeds of Datura stramonium and synthesis alkaloids in male rats. J Med Plant Res. 2011; 5(15):3421-3431.

Bruneton J. Toxic plants: Plants dangerous to humans and animals. (3rd Ed.). Paris : Lavoisier ; 2005.

Kurt R. Chromatography on thin layers. (1st ed.). Paris : Gauthiers Villars; 1971.

Allouni R, Guergour H, Mahdeb N, Omrane M, Bouzidi A. Acute Toxicity Study of the Total Alkaloids of Ruta montana in Male Mice. Int J Pharmacogn Phytochem Res. 2017; 9(8):1060- 1066.

Kartal M, Altun ML, Kurucu S. HPLC method for the analysis of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L. J Pharm Biomed Anal. 2003; 31(2):263-269.

Bensalem S, Soubhye J, Aldib I, Bournine L, Tho Nguyen A, Vanhaeverbeek M, Rousseau A, Boudjeltia K, Sarakbi A, Kauffmann J, Nève J, Prévost M, Stévigny C, Maiza- Benabdesselam F, Bedjou F, Van Antwerpen P, Duez P. Inhibition of myeloperoxidase activity by the alkaloids of Peganum harmala L. (Zygophyllaceae). J Ethnopharmaco. 2014; 154:361-369.

Lamchouri F, Settaf A, Cherrah Y, El Hamidi M, Tligui NS, Lyoussi B, Hassar M. Experimental toxicity of Peganum harmala seeds. Ann. pharm. Fr. 2002; 60(2):123-129.

Frison G, Favretto D, Zancanaro F, Fazzin G, Ferrara SD. A case of b-carboline alkaloid intoxication following ingestion of Peganum harmala seed extract. Forensic Sci Int. 2008; 179(2- 3):37–43.

Abbassi K, Mergaoui L, Atay Z, Adiri K, Stambouli A, Ghaout S. Effects of Peganum harmala extracts (Zygophyllaceae) on Desert Locust (Schistocerca gregaria Forskål 1775). Zool Baetica. 2003; 13/14:203-217.

Aarons DH, Rossi GV, Orzechowski RF. Cardiovascular actions of three harmala Alkaloids: Harmine, harmaline, and harmalol. J Pharm Sci. 1977; 66(9):1244-1248.

Shamaki BU, Sandabe UK, Abdulrahaman FI, Ogbe AO, Hassan ZI, Yusuf IL, Bitrus W, Zongoma Y, Isikhuemhen O. Toxicity studies and body weights changes in Wistar rats following oral administration of methanol extract from indigenous ganoderma sp. In Nigeria. MOJ Biol Med. 2017; 1(5):138‒141.

Kifayatullah M, Mohd SM, Pinaki S, Moklesur MRS, Arindam D, Sreemoy KD. Evaluation of the acute and sub-acute toxicity of the ethanolic extract of Pericampylus glaucus (Lam.) Merr. in BALB/c mice. J Acute Dis. 2015; 4(4):309–315.

Miglio A, Gavazza A, Siepi D, Bagaglia F, Ambra MA, Antognoni MT. Hematological and Biochemical Reference Intervals for 5 Adult Hunting Dog Breeds Using a Blood Donor Database. Animals (Basel). 2002; 10(7):1212.

Moore DM, Zimmerman K, Smith SA. Hematological Assessment in Pet Rabbits: Blood Sample Collection and Blood Cell Identification. Vet. Clin. N. Am. - Exot. Anim. Pract. 2015; 18(1):9-19.

Barnes JM and Denz FA. Experimental methods used in determining chronic toxicity. A critical review. Pharmacol Rev. 1954; 6(2):191-242.

Xin Rh , Wen-jing P, Xiao-lei L, Yong-jiang L, Gui-bo W, Chao- ying L, Jia-sheng X, Jin-yu L, Ge L, Ji-fang Z. Evaluation of the acute and subchronic toxicity of Ziwan aibu tang. Afr J Tradit Comple Altern Med. 2016; 13(3):140-149.

Blétry O and Marroun I. From symptom to prescription in general medicine Symptoms – Diagnosis – Therapeutics. (2nd ed.). Paris: Elsevier Masson, 2014.

Lauda MM, Perier A, Souchaud-Debouverie O, Roy-Peaud F, Luca L, Landron C, Roblot P, Martin M. Isolated ASAT elevation: a simple diagnosis to evoke. Rev Med Interne. 2018; 39(S2):A103-A2035.

Mohamed AS, Jawad AL, Jammali SM, Naki ZJ. Effect of repeated administration of Peganum harmala alcoholic extract on the liver and kidney in Albino mice: A histo-pathological study. J Sci Innov Res. 2013; 2(3):585-597.

Weiner D, Mitch WE, Sands JM. Urea and Ammonia Metabolism and the Control of Renal Nitrogen Excretion. Clin J Am Soc Nephrol. 2015; 10(8):1444–1458.

Higgins C. Urea and the clinical value measuring blood urea concentration. 2016 [cited July 2016]. Available from: www.acutecaretesting.org.

Shapira Z, Terkel J, Egozi Y, Nyska A, Friedman J. abortifacient potential for the epigeal parts of Peganum harmala. J Ethnopharmacol. 1989; 27(3):319-325.

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Published

2022-10-01

How to Cite

Nadia, M., Rima, A., Rima, R., khouloud, A., & Abdelouahab, B. (2022). Evaluation of the Subacute Toxic Effects of the Alkaloids of the Seeds of Peganum harmala L in the Liver, Kidney and Ovary of Female Rats: http://www.doi.org/10.26538/tjnpr/v6i10.12. Tropical Journal of Natural Product Research (TJNPR), 6(10), 1632–1637. Retrieved from https://tjnpr.org/index.php/home/article/view/1215