Bioactivity Endophytic Fungi Isolated from the Leaf Stalk of Syzygium jambos L. Alston http://www.doi.org/10.26538/tjnpr/v6i11.4
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Abstract
Antibiotic resistance and free radicals are a problem to be overcome by using antibiotics and antioxidants from natural ingredients. This study aimed to investigate the antibacterial and antioxidant activity of endophytic fungi (EF) isolated from the leaf stalks of jambu mawar (Syzygium jambos (L.) Alston). EF isolated from the leaf stalk of S. jambos was identified microscopically (shape of hyphae and spores) and macroscopically (growth patterns, colony color, texture, margin, and other characteristics). Antibacterial activity was tested on Salmonella typhi, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis bacteria using the Kirby Bauer method. Antioxidant test using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The molecular identified of EF with high antioxidant and antibacterial activity to determine the species and continued with the isolation of pure compounds. Identification of pure compounds was carried out by spectroscopic methods including 1D NMR (¹H-NMR and C-NMR). The results of the isolation of EF from the leaf stalk of S. jambos found four isolates, namely SJS1- SJS4. The results of the antibacterial test represented that the EF isolate SJS1 had strong activity against S. aureus and B. subtilis bacteria. The antioxidant activity test showed IC50 value of 29.29 g/mL. Molecular identification results showed that SJS1 was identified as Lasiodiplodia theobromae. Spectroscopic results of the pure compound identified as 3,5-dihydroxy-4-(4-
hydroxyphenyl)tetrahydro-2H-pyran-2-one. The endophytic fungus Lasiodiplodia theobromae was isolated from the leaf stalk of S. jambos (L.) Alston has the potential as a source of antioxidants and antibacterial bioactive compounds that can be developed through further research, including in vitro and in vivo tests.
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