Pharmacognostic Study of Bawang Dayak (Eleutherine bulbosa (Mill.) Urb.) and its Clay Mask Against Acne-Causing Bacteria


  • Syahrida D. Ardhany Department of Pharmacy, Faculty of Health Sciences Muhammadiyah University of Palangkaraya, Palangka Raya, 73111, Central Kalimantan, Indonesia
  • Muhammad A. Fansuri Department of Pharmacy, Faculty of Health Sciences Muhammadiyah University of Palangkaraya, Palangka Raya, 73111, Central Kalimantan, Indonesia
  • Susi Novaryatiin Department of Pharmacy, Faculty of Health Sciences Muhammadiyah University of Palangkaraya, Palangka Raya, 73111, Central Kalimantan, Indonesia


Acne,, Bawang Dayak,, Clay Mask,, Eleutherine bulbosa,, Pharmacognostic.


Previous studies have shown that the ethanolic extract of Bawang Dayak (Eleutherine bulbosa (Mill.) Urb.) can inhibit acne-causing bacteria. Therefore, this study aims to conduct a pharmacognostic evaluation of Bawang Dayak and make a clay mask for acne vulgaris. Bawang Dayak bulbs were extracted using a percolator with 96% ethanol. The qualitative test is carried out using several methods, including the identification of organoleptic, macroscopic, microscopic, and chemical compounds with thin-layer chromatography. Furthermore, the physical and microbiology test was conducted on the clay mask using the disc diffusion technique against acne-causing bacteria. The result showed the physico-chemical parameters of bulbs as 8.67% loss on drying, 0.21% foreign organic matter, 13.31% water-soluble, 10.52% alcohol soluble, 0.53% total ash, 0.85% acid insoluble ash, and 1.67% water-soluble ash. The qualitative test of the phytochemical compound showed the presence of alkaloids, flavonoids, saponins, tannins, and catechols. The best eluent TLC for the ethanolic extract was n-hexane: ethyl acetate (8:2) or ethyl acetate: ethanol: water (8:2:1). Furthermore, the clay mask has moderate activity against S. aureus and S. epidermidis and weak activity against P.acnes. Conclusively, pharmacognostic studies on Bawang Dayak simplicia bulbs met the set parameters, and the clay mask of Bawang Dayak inhibits acne-causing bacteria.


Rani SV and Nair BR. Pharmacognostic and physicochemical evaluation of bulbs of Eleutherine bulbosa (Mller) Urban, a medicinal plant. J Pharmacogn Phytochem. 2015; 4(3):273-77.

Fitriyanti, Yusmalina, Muthia R. Pharmacognostic study of sangkareho root (Callicarpa longifolia Lam.) from Pelaihari, South Kalimantan. Borneo J Pharm. 2020; 3(2):58-63.

Majid N, Nissar S, Raja WY, Nawchoo IA, Bhat ZA. Pharmacognostic standardization of Aralia cachemirica: a comparative study. Future J Pharm Sci. 2021; 7(33):1-8.

Muthia R, Wati H, Jamaludin WB, Kartini, Setiawan F, Fikri M, Wahhab A. Standardization of Eleutherine bulbosa Urb. bulbs and total flavonoid content from three locations in Kalimantan, Indonesia. Pharmacogn J. 2021; 13(1):73-80.

Kamarudin AA, Sayuti NH, Saad N, Razak NAA, Esa NM. Induction of apoptosis by Eleutherine bulbosa (Mill.) Urb. bulb extracted under optimised extraction condition on human retinoblastoma cancer cells (WERI-Rb-1). J Ethnopharmacol. 2022; 284(114770).

Kamarudin AA, Sayuti NH, Saad N, Razak NAA, Esa NM. Eleutherine bulbosa (Mill.) Urb. Bulb: Review of the pharmacological activities and its prospects for application. Int J Mol Sci. 2021; 22(6747):1-20.

Novaryatiin S and Ardhany SD. Potential anti-acne: bawang dayak (Eleutherine bulbosa (Mill.) Urb.) from Central Kalimantan-Indonesia. Pharmacogn J. 2020; 12(1):52-57.

Novaryatiin S and Ardhany SD. The antibacterial activity of bawang dayak (Eleutherine bulbosa (Mill.) Urb.) from Central Kalimantan against acne-causing bacteria. Int J Appl Pharm. 2019; 11(Special issue 5):22-25.

Ardhany SD. Antibacterial activity of bawang dayak (Eleutherine sp.) and Tawas Ut (Ampelocissus sp.) from Central Kalimantan against Propionibacterium acnes. Int J Appl Pharm. 2019; 11(Special issue 3):7-10.

Ardhany SD, Putra CD, Novaryatiin S. Modification of anti- acne bawang dayak (Eleutherine bulbosa (Mill.) Urb.) cream to Propionibacterium acnes. J Adv Pharm Technol Res. 2021; 12(1):94-98.

Ardhany SD, Novaryatiin S, Pratama MRF, Utar Z. Irritation test of bawang dayak (Eleutherine bulbosa (Mill.) Urb.) extract cream with human patch test method. J Farmasi Sains dan Praktis (JFSP). 2021; 7(1):74-80.

Ardhany SD, Effendie RR, Novaryatiin S. Uji iritasi formulasi sediaan krim ekstrak bawang dayak (Eleutherine bulbosa (Mill.) Urb.) pada kelinci albino putih. Jurnal Surya Medika. 2019; 5(1):63-9.

Ardhany SD and Novaryatiin S. Antibacterial activity of ethanolic extract bawang dayak (Eleutherine bulbosa (Mill.) Urb,) in cream against Propionibacterium acnes. Int J Appl Pharm. 2019; 11(Special issue 5):1-4.

Angelina M, Mardhiyah A, Dewi RT, Fajriah S, Muthiah N, Ekapratiwi Y, Dewijanti ID, Sukirno, Jamilah, Hartati S. Physicochemical and phytochemical standardization, and antibacterial evaluation of Cassia alata leaves from different locations in Indonesia, Pharmacia. 2021; 68(4):947-56.

Gupta PC, Sharma N, Rao CV. Pharmacognostic studies of the leaves and stem of Careya arborea Roxb. Asian Pac J Trop Biomed.2012; 2(5):404-8.

Thamkaew G, Sjöholm I, Galindo FG. A review of drying methods for improving the quality of dried herbs. Crit Rev Food Sci Nutr. 2021; 61(11):1763-86.

Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med. 2018; 13(20):1-26.

Genovese C, Angeli FD, Bellia F, Distefano A, Spampinato M, Attanasio F, Nicolosi D, Salvatore VD, Furno DL, Mannino G, Milardo F, Volti GL. In vitro antibacterial, anti-adhesive and anti-biofilm activities of Krameria lappacea (Dombey) Burdet & BB simpson root extract against methicillin-resistant Staphylococcus aureus strains. Antibiotics. 2021; 10(428):1-21.

Sakr MM, Ibrahim N, Ali S, Alzahaby N, Omar A, Khairy W, Zohdy BW, Qassem O, Saleh SE. Identification of potential quorum quenching compounds in Brassica oleracea var. capitata against MDR Pseudomonas aeruginosa and Escherichia coli clinical isolates. Arch Pharm Sci ASU. 2021; 5(1):128-142.

Vikhe S and Kunkulol R. Microscopic investigation and pharmacognosy of Stringa orobanchioides Benth. Pharmacogn J. 2020; 12(6):1325-31.

Pandiyan R and Ilango K. Pharmacognostical, physicochemical and phytochemical evaluation of Huberantha senjiana (Annonaceae) leaf: an endemic tree of Gingee Hills Tamil Nadu India. J Pharm Pharmacogn Res. 2022; 10 (1):158-72.

Arnida M, Khairunnisa A, Sutomo F. Standardization of simplicia and ethanol extract of Purun Danau (Lepironia articulate (Retz.) Domin) Rhizome. Borneo J Pharm. 2021; 4(4):273-82.

Jayani NIE, Krisnawan AH, Oktaviyanti ND, Kartini. Standardization of Phyllanthus niruri and Sonchus arvensis as components of scientific Jamu. Trad Med J. 2020; 25(1):7-14.

Husni E and Awaliana FI. Standardization extracts and simplicia of Limau Sundai peel (Citrus x aurantiifolia ‘sundai’) determine content of nobiletin and antibacterial activity test. Pharmacogn J.2021; 13(6):1323-31.

Evifania RD, Apridamayanti P, Sari R. Uji parameter spesifik dan nonspesifik simplisia daun senggani (Melastoma malabathricum L.). Jurnal Cerebellum. 2020; 6(1):17-20.

Yan Y, Li X, Zhang C, Lv L, Gao B, Li M. Research progress on antibacterial activities and mechanisms of natural alkaloids: a review. Antibiotics. 2021; 10(318):1-30.

Shamsudin NF, Ahmed QU, Mahmood S, Shah SAA, Khatib A, Mukhtar S, Alsharif MA, Parveen H, Zakaria ZA. Antibacterial effects of flavonoids and their structure-activity relationship study: a comparative interpretation. Molecules. 2022; 27(1149):1-43.

Adamczak A, Ozarowski M, Karpinski TM. Antibacterial activity of some flavonoids and organic acids widely distributed in plants. J Clin Med. 2020; 9(109):1-17.

Tagousop CN, Tamokou JDD, Kengne IC, Ngnokam D, Nazabadioko LV. Antimicrobial activities of saponins from Melanthera elliptica and their synergistic effects with antibiotics against pathogenic phenotypes. Chem Cent J. 2018; 12(97):1-9.

Eylands NJ, Evans MR, Shaw AM. Antimicrobial mitigation via saponin intervention on Escherichia coli and growth and development of hydroponic lettuce. HortTechnoloy. 2021; 31(2):174-80.

Maisetta G, Batoni G, Caboni P, Esin S, Rinaldi AC, Zucca P. Tannin profile, antioxidant properties, and antimicrobial activity of extracts from two Mediterranean species of parasitic plant Cytinus, BMC Complement Alter Med. 2019; 19(82):1- 11.

Puljula E, Walton G, Woodward MJ, Karonen M. Antimicrobial activities of Ellagitannins against Clostridiales perfringens, Escherichia coli, Lactobacillus plantarum, and Staphylococcus aureus. Molecules. 2020; 25(16):1-13.

Dong S, Yang X, Zhao L, Zhang F, Hou Z, Xue P. Antibacterial activity and mechanism of action saponins from Chenopodium quinoa Willd. husks against foodborne pathogenic bacteria. Ind Crops Prod. 2020; 149:112350.

Farha AK, Yang QQ, Kim G, Li HB, Zhu F, Liu HY, Gan RY, Corke H. Tannins as an alternative to antibiotics. Food Biosci. 2020; 38:100751.

Razaviamri S, Wang K, Liu B, Lee BP. Catechol-based antimicrobial polymers. Molecules. 2021; 26(3):1-17.

Fridayanti A, Purwanto DA, Hendradi E. Preliminary phytochemical screening and GC-MS analysis of ethanol extract of bulbs of Eleutherine sp. Trop J Nat Prod Res. 2022; 6(3):361-64.

Akhtar MS, Rafiullah M, Shehata WA, Hossain A, Ali M. Comparative phytochemical, thin layer chromatographic profiling and antioxidant activity of extracts from some Indian herbal drugs. J Bioresour Bioprod. 2022; 14(22):1-7.

Werdyani S, Fitria A, Rakhmawati S. In vitro tracing of cytotoxic compounds in Jarak Cina Stem Bark (Jatropha Multifida Linn.). EKSAKTA J Sci Data Analysis. 2020; 1(1):7- 13.

Dewatisari WF, Nugroho LH, Retnaningrum E. The potency of Sansevieria trifasciata and S. cylindrical leaves extracts as an antibacterial against Pseudomonas aeruginosa. Biodiversitas.2021; 22(1):408-15.

Lukic M, Pantelic I, Savić SD. Towards optimal pH of the skin and topical formulations: from the current state of the art to tailored products. Cosmetics. 2021; 8(3), 69:1-18.

Syamsidi A, Syamsuddin AM, Sulastri E. Formulation and antioxidant activity of clay mask of tomato (Solanum lycopersicum L.) lycopene extract with variation of concentration of kaoline and bentonite bases. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy). 2021; 7(1):77-90.

Shahbazi Y. Antibacterial and antioxidant properties of methanolic extracts of apple (Malus pumila), Grape (Vitis vinifera), Pomegranate (Punica granatum L.), and Common fig (Ficus carica L.) fruits. Pharm Sci. 2017; 23(4):308-15.




How to Cite

D. Ardhany, S., A. Fansuri, M., & Novaryatiin, S. (2022). Pharmacognostic Study of Bawang Dayak (Eleutherine bulbosa (Mill.) Urb.) and its Clay Mask Against Acne-Causing Bacteria: Tropical Journal of Natural Product Research (TJNPR), 6(10), 1614–1621. Retrieved from