The Combined Effect of Coenzyme Q10 and Magnesium Ion on Advanced Glycation End Products Formed by Methylglyoxal in Rabbits http://www.doi.org/10.26538/tjnpr/v6i10.7
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Abstract
Methylglyoxal (MGO) is a poisonous and highly reactive alpha-oxoaldehyde with increased levels during oxidative stress in various disorders. MGO is generated from glyceraldehyde-3- phosphate and dihydroxyacetone phosphate during glycolysis. Continued exposure to Methylglyoxal causes Advanced Glycation End products (AGEs), implicated in different diseases, such as the early stages of diabetes, and affects male fertility, reducing sperm count and quality. This study aimed to evaluate the combined effects of coenzyme Q10 and Magnesium ion on oxidoreductase and liver enzymes due to the toxicity of MGO in experimental animals. Twenty-four rabbits (male and female) weighing 750-2100 mg, divided into four groups, were used for this experiment. Group one was used as the positive control (without treatment). Group two (negative control) received an intraperitoneal dose of 0.1 ml (20 mg/kg) per kg body weight of Methylglyoxal. Animals in group three received 0.1 ml (20 mg/kg) methylglyoxal intraperitoneally and Magnesium 5 mg/kg orally per kg body weight. Group four animals received 0.1 ml (20 mg/kg) methylglyoxal intraperitoneal, a combination of 5 mg/kg Magnesium, and 2.5 mg/kg coenzyme Q10 orally to evaluate the effect of the metal ions and antioxidants on liver enzymes activity and uric acid and other endogenous antioxidant markers (SOD and GSH). Results from our study showed that methylglyoxal exerted toxic effects on some biochemical parameters of the animals. However, the administration of CoQ10 and Magnesium ions significantly increased the levels of liver and antioxidant enzymes such as SOD and glutathione levels to resist oxidative overload.
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Lee JH, Parveen A, Do MH, Kang MC, Yumnam S, Kim SY. Molecular mechanisms of methylglyoxal-induced aortic endothelial dysfunction in human vascular endothelial cells. Cell Death Dis. 2020; 11(5):1-15.
Zheng J, Guo H, Ou J, Liu P, Huang C, Wang M, Simal-Gandara J, Battino M, Jafari SM, Zou L, Ou S, Xiao J. Benefits, deleterious effects and mitigation of methyllyoxal in foods: A critical review. Trends Food Sci Technol. 2021; 107:201–212.
Bellier J, Nokin MJ, Lardez E, Karoyan P, Peulen O, Castronovo V, Bellahcene A. Methylglyoxal, a potent inducer of AGEs, connects between diabetes and cancer. Diabetes Res Clin Pract. 2019; 148:200-211.
Matafome P, Rodrigues T, Sena C, Seiça R. Methylglyoxal in Metabolic Disorders: Facts, Myths, and Promises. Med Res Rev. 2017; 37(2):368-403.
Mahdi NK, Mahdi JK, S.Abd Al-Wahab SA. Oxidative Stress Among Patients with Some Different Parasitic Infections. Med J Basrah Univ. 2009; 27(2):66-70.
Al-ubuda NM, Al-naama LM, Al-hashimi AH. Profile study of some oxidant and antioxidant levels in leukemic patients. Med J Basrah Univ. 2012; 30(2):115-121.
Schumacher D, Morgenstern J, Oguchi Y, Volk N, Kopf, S, Groenerm JB, Nawroth PP, Fleming T, Freichel M. Compensatory mechanisms for methylglyoxal detoxification in experimental & clinical diabetes. Mol Metab. 2018; 18:143-152.
Schmitz AE, de Souza LF, Santos B, Maher P, Lopes FM, Londero GF, Klamt F,Dafre AL. Methylglyoxal-Induced Protection Response and Toxicity: Role of Glutathione Reductase and Thioredoxin Systems. Neurotox Res. 2017; 32(3):340-350.
Pal A, Roy A, Ray M. Creatine supplementation with methylglyoxal: a potent therapy for cancer in experimental models. Amino Acids. 2016; 48(8):2003-2013.
Michel M, Hess C, Kaps L, Kremer WM, Hilscher M, Galle PR, Moehler M, Schattenberg JM, Worns MA, Labenz C, Nagel M. Elevated serum levels of methylglyoxal are associated with impaired liver function in patients with liver cirrhosis. Sci Rep. 2021; 11(1):1-11.
Pisoschi AM and Pop A. The role of antioxidants in the chemistry of oxidative stress: A review. Eur J Med Chem. 2015; 97:55-74.
Paramita D and Wisnubroto JD. Effect of methylglyoxal on reactive oxygen species, KI-67, and caspase-3 expression in MCF-7 cells. Exp Mol Pathol. 2018; 105(1):76-80.
Prestes A, Santos MM, Ecker A, Zanini D, Schetinger MC, Rosemberg DB, de Rocha JB, Barbosa NV. Evaluation of methylglyoxal toxicity in human erythrocytes, leukocytes and platelets. Toxicol Mech Methods. 2017; 27(4):307-317.
Polykretis P, Luchinat E, Boscaro F, Banci L. Methylglyoxal interaction with superoxide dismutase 1. Redox Biol. 2020; 30:101421.
Hameed BJ, Abood HS, Ramadhan UH. Antihyperuricemic and xanthine oxidase inhibitory activities of silymarin in a rat gout model. Int J Green Pharm. 2018; 12(3):S695-S699.
Narainsamy K, Farci S, Braun E, Junot C, Cassier-Chauvat C, Chauvat F. Oxidative-stress detoxification and signalling in cyanobacteria: The crucial glutathione synthesis pathway supports the production of ergothioneine and ophthalmate. Mol Microbiol. 2016; 100(1):15-24.
Hernández-Camacho JD, Bernier M, López-Lluch G, Navas P. Coenzyme Q10 supplementation in aging and disease. Front Physiol. 2018; 9:1-11.
Schwalfenberg GK and Genuis SJ. The Importance of Magnesium in Clinical Healthcare. Scientifica (Cairo). 2017; 2017:4179326.
Girin SV, Savinova IV, Chervinska TM, Antonenko IV, Naumenko NV. Glyoxal and methylglyoxal ultra-low doses activate lymphocytes energy and nitrogen metabolism in vitro. Res J Pharm Biol Chem Sci. 2016; 7(4):1831-1839.
Kostov K. Effects of magnesium deficiency on mechanisms of insulin resistance in type 2 diabetes: Focusing on the processes of insulin secretion and signalling. Int J Mol Sci. 2019; 20(6):1351.
Saleh AS, Shahin MI, Kelada NA. Hepatoprotective effect of taurine and coenzyme Q10 and their combination against acrylamide-induced oxidative stress in rats. Trop J Pharm Res. 2017; 16(8):1849-1855.
Seo K, Seo S, Han JY, Ki SH, Shin SM. Resveratrol attenuates methylglyoxal-induced mitochondrial dysfunction and apoptosis by Sestrin2 induction. Toxicol Appl Pharmacol. 2014; 280(2):314-322.
Gutierrez-Mariscal FM, Yubero-Serrano EM, Villalba JM, Lopez-Miranda J. Coenzyme Q10: From bench to clinic in aging diseases, a translational review. Crit Rev Food Sci Nutr. 2019; 59(14):2240-2257.
Nahar K, Hasanuzzaman M, Alam MM, Fujita M. Roles of exogenous glutathione in antioxidant defense system and methylglyoxal detoxification during salt stress in mung bean.Biol Plant. 2015; 59(4):745-756.
Casagrande D, Waib PH, JordãoJúnior AA. Mechanisms of action and effects of the administration of Coenzyme Q10 on metabolic syndrome. J Nutr Intermed Metab. 2018; 13:26-32.
Farsi F, Mohammadshahi M, Alavinejad P, Rezazadeh A, Zarei M, Engali KA. Functions of Coenzyme Q10 Supplementation on Liver Enzymes, Markers of Systemic Inflammation, and Adipokines in Patients Affected by Non-alcoholic Fatty Liver Disease: A Double-Blind, Placebo-Controlled, Randomized Clinical Trial. J Am Coll Nutr. 2016; 35(4):346-353.
Maheshwari R, Balaraman R, Sen AK, Shukla D, Seth A. Effect of concomitant administration of coenzyme Q10 with sitagliptin on experimentally induced diabetic nephropathy in rats. Ren Fail. 2017; 39(1):130-139.
Sari MI, Daulay M, Wahyuni DD. Superoxide Dismutase Levels and Polymorphism (Ala16val) in Tuberculosis Patients with Diabetes Mellitus in Medan City. Macedonian J Med Sci. 2019; 7(5):730-735.
Kammerscheit X, Hecker A, Rouhier N, Chauvat F, Cassier- Chauvat C. Methylglyoxal detoxification revisited: Role of glutathione transferase in model cyanobacterium Synechocystis sp. strain PCC 6803.MBio. 2020; 11(4):1-13.
Tuama R, Shari F, Ramadhan U. The Effect of Antioxidants on Electrolytes in Vancomycin- Streptozotocin Induced Diabetes Kidney Disease in Rabbits. Indian J Forensic Med Toxicol. 2021; 15(3):3971-3978.