Traditional Uses, Botany, Phytochemistry, and Pharmacology of Pelargonium graveolens: A Comprehensive Review http://www.doi.org/10.26538/tjnpr/v6i10.2

Main Article Content

Harzallah A. Amel
Hachama Kamel
Fizir Meriem
Khadraoui Abdelkader

Abstract

Pelargonium graveolens (PG) is a popular medicinal plant, widely used in Africa for centuries to treat various diseases. Because of its wide exploitation, the therapeutic studies of P. graveolens keep penetrating. This research engulfs a comprehension of all previous studies related to this medicinal herb, where it summaries and evaluates the traditional uses, the botany, the phytochemistry, the pharmacology, and the toxicology of P. graveolens. A literature review was conducted through the classic books of herbs, medicine, PhD papers, and online scientific databases, searching up to January 2022. This review analyzes all literature on the research subject. Our main findings are: (1) more than 290 biochemical components have been identified from P. graveolens, counting terpenoids, flavonoids, steroids, alkaloids and other composites. (2) Terpenoids are the most significant biologically active matter detected in this plant. (3) Extracts and compounds of P. graveolens exert a wide range of pharmacological effects. Study of the plant’s toxicological effects has also been restricted as well. P. graveolens has potential in the treatment of numerous ailments, particularly cancers and diabetes. Current investigations confirmed that much traditional uses of P. graveolens has been corroborated by current studies. However, contemporary reports on its pharmacological impacts are not deep enough, and its underlying mechanisms for the cure of tumours and diabetes should be further elucidated.

Article Details

How to Cite
A. Amel, H., Kamel, H., Meriem, F., & Abdelkader, K. (2022). Traditional Uses, Botany, Phytochemistry, and Pharmacology of Pelargonium graveolens: A Comprehensive Review: http://www.doi.org/10.26538/tjnpr/v6i10.2. Tropical Journal of Natural Product Research (TJNPR), 6(10), 1547-1569. https://tjnpr.org/index.php/home/article/view/1195
Section
Articles

References

Blerot B, Baudino S, Prunier C, Demarne F, Toulemonde B, Caissard JC. Botany, agronomy and biotechnology of Pelargonium used for essential oil production. Phytochem Rev. 2016; 15(5):935-960.

Bown D. New Encyclopedia of Herbs and Their Uses: The Definitive Guide to the Identification, Cultivation, and Uses of Herbs the Definitive Guide to the Identification, Cultivation and Uses of Herbs. New York: Dorling Kindersley; 2001; 173- 195p.

Tucker AO. Scented-leaf Geraniums: Introduction & Recipes. Deware: Department of Agriculture and Natural Resources, Delaware State University; 1991; 16p.

Miller D. Pelargoniums: A Gardener's Guide to the Species and Their Cultivars and Hybrids. New York: Timber Press; 1996; 48p.

Saraswathi J, Venkatesh K, Nirmala B, Majid HH, Roja Rani A. Phytopharmacological importance of Pelargonium species. J Med Plant Res. 2011; 5(13):2587-2598.

Lis-Balchin M. Geranium and Pelargonium: History of Nomenclature, Usage and Cultivation. Abingdon-on-Thames: Taylor & Francis Group; 2019; 6-32p.

Palmer E and Clarke B. The South African Herbal. Cape Town: Tafelberg Publishers; 1985; 58-72p.

Brawner F. Geraniums: The Complete Encyclopedia. London: Schiffer Publishing, Limited; 2003; 3-16p.

Lavasanijou MR, Reza M, Sohrabi HR, Karimi M, Ashjazade M, Salajeghe M, Farzineejadizadeh H, Parsaei P, Elmamooz A. Wound healing effects of quercus brantii and Pelargonium graveolens extracts in male wistar rats. Wounds 2016; 28(10):369-375.

Roeschenbleck J, Albers F, Müller K, Weinl S, Kudla J, Phylogenetics, character evolution and a subgeneric revision of the genus Pelargonium (Geraniaceae). Phytotaxa; 2014; 159(2):31-76.

Van-der-Walt JJ. A taxonomic revision of the type section of Pelargonium L’Hérit.(Geraniaceae). Bothalia 1985; 15(3):345- 385.

Acevedo-Rodríguez P and Strong MT. Catalogue of seed plants of the West Indies. California: Smithsonian Institution Scholarly Press; 2012; 153-968p.

Belsinger S and Coca J. Flowers in the Kitchen: A Bouquet of Tasty Recipes. Colorado: Interweave Press; 1991; 14-18p.

Boukhatem M, Sudhaa T, Darwish NHE, Nada HG, Mousaa SA. Aromatic essence of Geranium Scented (Pelargonium graveolens L'Hérit.) from Algeria: exploration of antioxidant, anti-inflammatory and anticancer (anti-angiogenic and cytotoxic) properties, in vitro and in ovo, against different cell lines metastatic cancer. Ann pharm fr. 2021; 80(3):383-396.

Ringelberg J, Bakker F, van-der-Niet T. Floral evolution in Cape Pelargonium (Geraniaceae). Wageningen University: Biosystematics Group; 2012; 53-41p.

Gupta R, Sastry KP, Banerjee S, Mallavarapu GR, Kumar S. Genetic resource enhancement by isolation of diversegenotypes from seed progeny in predominantly sterile rose scentedgeranium Pelargonium graveolens. Genet Resour Crop Evol. 2001; 48(6):629-636.

Gardner Z, McGuffin M, American Herbal Products Association’s Botanical Safety Handbook, Second Edition. New York: Taylor & Francis; 2013; 335p.

Lawless J. The Encyclopedia of Essential Oils: The Complete Guide to the Use of Aromatic Oils In Aromatherapy, Herbalism, Health, and Well Being. Massachusetts: Red Wheel Weiser; 2013; 80-96p.

Leung AY and Foster S. Encyclopedia of Common Natural Ingredients: Used in Food, Drugs, and Cosmetics. Michigan: Wiley; 2003; 269-270p.

Paulsen E, Skov PS, Andersen KE. Immediate skin and mucosal symptoms from pot plants and vegetables in gardeners and greenhouse workers. Contact Derm. 1998; 39(4):166-170.

Barceloux DG. Medical Toxicology of Natural Substances: Foods, Fungi, Medicinal Herbs, Plants, and Venomous Animals. New Jersey: John Wiley & Sons; 2008; 64-68p.

Rinzler CA. The New Complete Book of Herbs, Spices, and Condiments. New York: Facts on File; 2001; 77-80p.

Peroni L and Pistoia M. The Language of Flowers. New York: Crown Publishers; 1985; 128-194p.

Peddie M, Lewis J, Lewis J. Growing & Using Scented Geraniums: Storey's Country Wisdom Bulletin A-131. North Adams: Storey Publishing; 1991; 28p.

Barrett J. What Can I Do with My Herbs?: How to Grow, Use, and Enjoy These Versatile Plants. Texas: Texas A&M University Press; 2009; 63-67p.

Crowley C. 101+ Recipes from the herb lady. North Carolina: Lulu. com; 200; 76-81p.

Tümen G, Malyer H, Bafier K, Aydin S. Plants used in Anatolia for wound healing: Proceedings of the IVth international congress of ethnobotany (ICEB 2005); 2006; 217- 221p.

Mahboubi M, Taghizadeh M, Khamechian T, Tamtaji OR, Mokhtari R, Alireza S. The wound healing effects of herbal cream containing Oliveria decumbens and Pelargonium graveolens essential oils in diabetic foot ulcer model. World J Plast Surg. 2018; 7(1):45-50.

Van Wyk BE. The potential of South African plants in the development of new medicinal products. S Afr J Bot. 2011; 77(4):812-829.

Owen DJ. The Herbal Internet Companion: Herbs and Herbal Medicine Online. Florida: Taylor & Francis; 2002; 170-172p.

da Cunha AP, Nogueira MT, Roque OR. Plantas aromáticas e óleos essenciais: composição e aplicações. Lisboa: Fundação Calouste Gulbenkian; 2012; 520-670p.

San MAJ. Medicinal plants in central Chile. Econ Bot. 1983; 37(2):216-227.

Small E. Culinary Herbs. Ontario Canada: NRC Research Press; 2006; 356p.

Duke JA and Ayensu ES. Medicinal Plants of China. Washington: Reference Publications; 1985; 121p.

Prasad M. Trace elements in traditional healing plants— remedies or risks. Trace elements as contaminants and nutrients: consequences in ecosystems and human health. New York: Wiley; 2008; 137-160p.

Pittler MH and Ernst E. Complementary therapies for neuropathic and neuralgic pain: systematic review. The Clinical journal of pain 2008; 24(8):731-733.

Hart S and Lis-Balchin M. Pharmacology of Pelargonium essential oils and extracts in vitro and in vivo. Florida: CRC Press; 2002; 128-143p.

Boukhris M, Bouaziz M, Feki I, Sayadi S. Hypoglycemic and antioxidant effects of leaf essential oil of Pelargonium graveolens L’Hér. in alloxan induced diabetic rats. Lipids Health Dis. 2012; 11(1):1-10.

Kolodziej H. Pelargonium reniforme and Pelargonium sidoides: their botany, chemistry and medicinal use. Florida: CRC Press; 2002; 274-302p.

Seidel V and Taylor PW. In vitro activity of extracts and constituents of Pelagonium against rapidly growing mycobacteria. Int J Antimicrob Agents. 2004; 23(6):613-619.

Erb M and Kliebenstein DJ. Plant secondary metabolites as defenses, regulators, and primary metabolites: the blurred functional trichotomy. Plant physiol 2020; 184(1):39-52.

Pradeepa M, Kalidas V, Geetha N. Qualitative and quantitative phytochemical analysis and bactericidal activity of Pelargonium graveolens L’Her. Int J Appl Pharm 2016; 8(3):7- 11.

Ali E, Hassan F, Elgimabi M. Improving the growth, yield and volatile oil content of Pelargonium graveolens L. Herit by foliar application with moringa leaf extract through motivating physiological and biochemical parameters. S Afr J Bot. 2018; 119:383-389.

Khanuja SP, Shasany A, Darokar MP, Kumar S. Rapid isolation of DNA from dry and fresh samples of plants producing large amounts of secondary metabolites and essential oils. Plant Mol Biol Report. 1999; 17(1):74-74.

Ennaifer M, Bouzaiene T, Chouaibi M, Hamdi M, Pelargonium graveolens aqueous decoction: a new water-soluble polysaccharide and antioxidant-rich extract. Biomed Res Int. 2018; 2018(1)1-11.

Swamy K and Rao S. Effect of 24-epibrassinolide on growth, photosynthesis, and essential oil content of Pelargonium graveolens (L.) Herit. RJPPE2. 2009; 56(5):616-620.

El-Kareim A, El-Nagar M, Marouf AE. Attractiveness and Effects of Insectary Plant Flowers on Certain Aphidophagous Insects as Bio-Agents. JPPP. 2011; 2(6):609-622.

Ayad HS, El-Din K, Reda F. Efficiency of stigmasterol and α- tocopherol application on vegetative growth, essential oil pattern, protein and lipid peroxidation of geranium (Pelargonium graveolens L.). J Appl Sci Res. 2009; 5:887-892.

Kang CJ, Lee MG, Cho YS, Lee JW, Kyung YJ, Shin JS, Kim ES, Kim JK. Characterization of geranium (Pelargonium graveolens) chloroplast EF-Tu cDNA. Mol Cells. 2000; 10(5):579-583.

Hasanvand F, Rezaei Nejad A, Feizian M. Effect of Silicic Acid on some Anatomical and Biochemical Characteristics of Pelargonium graveolens under Salinity Stress. J Hortic Sci. 2017; 30(4):723-732.

Halees RY, Talib WH, Issa RA. Varthemia iphionoides and Pelargonium graveolens extracts as a treatment of breast cancer implanted in diabetic mice. Pharmacogn Mag. 2019; 15(65):698.

Ghorbanpour M and Hatami M. Changes in growth, antioxidant defense system and major essential oils constituents of Pelargonium graveolens plant exposed to nano-scale silver and thidiazuron. Indian J Plant Physiol. 2015; 20(2):116-123.

Heide L. Geranylpyrophosphate synthase from cell cultures of Lithospermum erythrorhizon. FEBS letters. 1988; 237(1- 2):159-162.

Lee SH, Kim ES, Lee MY. Purification and characterization of a cationic isoperoxidase from scented-geranium. Phytochem. 2001; 58(6):859-864.

Min BS, Kim YK, Ma CW, Jin ES, Lee TK, Lee YB, Ryo KK, Lee MY. Purification and Characterization of an Anionic Isoperoxidase from Scented‐Geranium Callus. Prep. Biochem. Biotechnol. 2004; 34(3):253-264.

Jain C, Khatana S, Vijayvergia R. Bioactivity of secondary metabolites of various plants: a review. Int J Pharm Sci. 2019; 10(2):494-498.

Yang L, Wen KS, Ruan X, Zhao YX, Wei F, Wang Q. Response of plant secondary metabolites to environmental factors. Mole. 2018; 23(4):762-786.

Paranamana V, Jayasekara H, Bulugahapitiya V. Phytochemical profile and In vitro sun protective activity of Wrightia antidysenterica, Ipomoea pescaprae and Ipomoea aquatica flower extracts; Proceedings of 9th Ruhuna International Science & Technology Conference 2022; 1-2.

Seca AM and Pinto DC. Biological potential and medical use of secondary metabolites: Multidisciplinary Digital Publishing Institute 2019; 6(2):66-72.

Kabera JN, Semana E, Mussa AR, He X. Plant secondary metabolites: biosynthesis, classification, function and pharmacological properties. J Pharm Pharmacol. 2014; 2(7):377-392.

Asgarpanah J and Ramezanloo F. An overview on phytopharmacology of Pelargonium graveolens L. NISCAIR- CSIR. 2015;1-15.

Yves-Ren N. Citronellol and geraniol in geranium and rose oils. Perfumery & Essential Oil Rec. 1957; 48:118-120.

Kami T, Ȏtaishi S, Hayashi S, Matsuura T, A study on low- boiling compounds of essential oil of geranium species. Agric Biol Chem. 1969; 33(4):502-505.

Bergman, ME, Bhardwaj M, Phillips MA. Cytosolic geraniol and citronellol biosynthesis require a Nudix hydrolase in rose‐ scented geranium (Pelargonium graveolens). Plant J. 2021; 107:493–510.

Aziz ZA, Akil A, Mohd SSH, Alptug K, Muhammed M, Lokhat D, Mohd R, Magdah G, Kamal M, Ghulam A. Essential oils: extraction techniques, pharmaceutical and therapeutic potential-a review. Curr Drug Metab. 2018; 19(13):1100-1110.

Liao J. Geranium, Pelargonium graveolens P. capitatum cv Analysis of leaf aroma components of Attar of Rose-the effect of leaf age and bleaching treatment. Dissertation of Institute of Horticulture, National Taiwan University. 2008:1-85.

Nur Ç. Chemical Fingerprinting of the Geranium (Pelargonium graveolens) Essential Oil by Using FTIR, Raman and GC-MS Techniques. EJOSAT 2021; (25):810-814.

Shellie RA and Marriott PJ. Comprehensive two-dimensional gas chromatography-mass spectrometry analysis of Pelargonium graveolens essential oil using rapid scanning quadrupole mass spectrometry. Analyst. 2003; 128(7):879-883.

Putievsky E, Ravid U, Dudai N. The effect of water stress on yield components and essential oil of Pelargonium graveolens L. J Essent Oil Res. 1990; 2(3):111-114.

Korezawa N. Studies on the Yield Analysis of Essential Oil in Pelargonium species. J Trop Agric. 1960; 4(1):1-12.

Gupta R, Mallavarapu GR, Banerjee S, Kumar S. Characteristics of an isomenthone‐rich somaclonal mutant isolated in a geraniol‐rich rose‐scented geranium accession of Pelargonium graveolens. Flavour Fragr J. 2001; 16(5):319-324.

OhtaY, Nishimura K, Hirose Y. Studies on the Monoterpene Fraction of ―Geranium Oil‖ from Pelargonium roseum Bourbon. Agric Biol Chem. 1964; 28(1):5-9.

Allison RD. The biosynthesis of terpenes in Pelargonium graveolens. Oregon State University; 1963; 3-70.

Hiroaki K. Jar fermenter culture of adventitious bud tissue of Pelargonium odoratum. J Japan Soc Biosci and Biotech. 1986; 60(1):15-17.

Van-der-Walt J and Demarne F. Pelargonium graveolens and P. radens: a comparison of their morphology and essential oils. S Afr J Bot. 1988; 54(6):617-622.

Demarne F and Van der Walt J. Origin of the rose-scented Pelargonium cultivar grown on Réunion Island. S Afr J Bot. 1989; 55(2):184-191.

Rajeswara R, Sastry BRK, Rao EVP, Ramesh S. Variation in yields and quality of geranium (Pelargonium graveolens L'Hér. ex Aiton) under varied climatic and fertility conditions. J Essent Oil Res. 1990; 2(2):73-79.

Lis-Balchin M. Essential oil profiles and their possible use in hybridization of some common scented geraniums. J Essent Oil Res. 1991; 3(2):99-105.

Rajeswara R, Bhattacharya BRA, Kaul P, Chand S, Ramesh S. Changes in Profiles of Essential Oils of Rose-Scented Geranium (Pelargonium sp.) During Leaf Ontogeny. J Essent Oil Res. 1993; 5(3):301-304.

Mallavarapu GR, Rao EVS, Ramesh S, Narayana M. Chemical and Agronomical Investigations of a New Chemotype of Geranium. J Essent Oil Res. 1993; 5(4):433-438.

Southwell IA and Stiff IA. Chemical Composition of an Australian Geranium Oil. J Essent Oil Res. 1995; 7(5):545-547.

Rana VS, Juyal JP, Blazquez MA. Chemical constituents of essential oil of Pelargonium graveolens leaves. Int J Aromather. 2002; 12(4):216-218.

Rao BRR. Biomass yield, essential oil yield and essential oil composition of rose-scented geranium (Pelargonium species) as influenced by row spacings and intercropping with cornmint (Mentha arvensis L.f. piperascens Malinv. ex Holmes). Ind Crops Prod. 2002; 16(2):133-144.

Rajeswara RBR, Kaul PN, Syamasundar KV, Ramesh S. Water soluble fractions of rose-scented geranium (Pelargonium species) essential oil. Bioresour Technol. 2002; 84(3):243-246.

Gomes PB, Mata VG, Rodrigues AE. Characterization of Portuguese-Grown Geranium Oil (Pelargonium sp.). J Essent Oil Res. 2004; 16(5):490-495.

Saxena G, Banerjee S, Gupta R, Rahman L, Tyagi BR, Kumar S, Mallavaparu G, Ramesh S. Composition of the essential oil of a new isomenthone-rich variant of geranium obtained from geraniol-rich cultivar of Pelargonium species. J Essent Oil Res. 2004; 16(2):85-88.

Gauvin A, Lecomte H, Smadja J. Comparative investigations of the essential oils of two scented geranium (Pelargonium spp.) cultivars grown on Reunion Island. Flavour Fragr J. 2004; 19(5):455-460.

Horsey P. The return of Kenyan essential oils. Int J Aromather. 2005; 15(4):159-162.

Pant KP, Bisht PS, Nautiyal MC. Oil Profile of Rose Scented Geranium var. Bourbon grown in the Garhwal Himalaya (India). J Essent Oil-Bear Plants. 2005; 8(1):28-31.

Shawl A, Kumar T, Chishti N, Shabir S. Cultivation of Rose Scented Geranium ( Pelargonium sp.) as a Cash Crop in Kashmir Valley. Asian J Plant Sci. 2006; 5(4):673-675.

Mnif W, Dhifi W, Jelali N, Baaziz H, Hadded A, Hamdi N. Characterization of leaves essential oil of Pelargonium graveolens originating from Tunisia: chemical composition, antioxidant and biological activities. J Essent Oil-Bear Plants. 2011; 14(6):761-769.

Ćavar S and Maksimović M. Antioxidant activity of essential oil and aqueous extract of Pelargonium graveolens L’Her. Food control 2012; 23(1):263-267.

Hsouna AB and Hamdi N. Phytochemical composition and antimicrobial activities of the essential oils and organic extracts from pelargonium graveolens growing in Tunisia. Lipids Health Dis. 2012; 11(1):1-7.

Ghannadi A, Bagherinejad M, Abedi D, Jalali M, Absalan B, Sadeghi N. Antibacterial activity and composition of essential oils from Pelargonium graveolens L'Her and Vitex agnus- castus L. Iran J Microbiol. 2012; 4(4):171-176.

Boukhris M, Monique S, Simmondes SJ, Sayadi S, Bouaziz M. Chemical Composition and Biological Activities of Polar Extracts and Essential Oil of Rose-scented Geranium, Pelargonium graveolens. Phytother Res. 2013; 27(8):1206- 1213.

Boukhatem MN, Kameli A, Saidi F. Essential oil of Algerian rose-scented geranium (Pelargonium graveolens): Chemical composition and antimicrobial activity against food spoilage pathogens. Food Control. 2013; 34(1):208-213.

Sharopov FS, Zhang H, Setzer WN. Composition of geranium (Pelargonium graveolens) essential oil from Tajikistan. Am J Essent Oils Nat Prod. 2014; 2(2):13-16.

Wang M, Chittiboyina AG, Avonto C, Parcher JF, Khan I. Comparison of Current Chemical and Stereochemical Tests for the Identification and Differentiation of Pelargonium graveolens L'Hér.(Geraniaceae) Essential Oils: Analytical Data for (-)-(1S, 4R, 5S)-Guaia-6, 9-diene and (-)-(7R, 10S)-10-epi- γ-Eudesmol. Planta Med. 2014; 8(4):360-372.

Ahamad J and Uthirapathy S. Chemical characterization and antidiabetic activity of essential oils from Pelargonium graveolens leaves. ARO-The Scientific Journal of Koya University 2021; 9(1):109-113.

Rana V and Blazquez M. Essential oil composition of aerial parts of Pelargonium graveolens at different growth stages. Indian Perfum. 2010; 54:22-25.

Fayed SA. Antioxidant and anticancer activities of Citrus reticulate (Petitgrain Mandarin) and Pelargonium graveolens (Geranium) essential oil. Res J Agric & Bio Sci. 2009; 5(5):740-747.

Kaiser R. (5R*, 9S*)‐and (5R*, 9R*)‐2, 2, 9‐Trimethyl‐1, 6‐ dioxaspiro [4.4] non‐3‐ene and their Dihydro Derivatives as New Constituents of Geranium Oil. Helv Chim Acta. 1984; 67(5):1198-1203.

Ravid U and Putievsky E. The influence of harvest dates and leaf location on the essential oil content and major components of Pelargonium graveolens L. in IV International Symposium on Spice and Medicinal Plants. 1983; 144:159-165.

Boukhatem MN, Kameli A, Ferhat MA, Saidi F, Mekarnia M. Rose geranium essential oil as a source of new and safe anti- inflammatory drugs. LJM 2013; 8(1):1-7.

Kuzuyama T. Biosynthetic studies on terpenoids produced by Streptomyces. J Antibiot. 2017; 70(7):811-818.

Ninkuu V, Zhang L, Yan J, Fu Z, Yang T, Zeng H. Biochemistry of terpenes and recent advances in plant protection. J Serb Chem. 2021; 22(11):1-22.

Bergman ME, Chávez Á, Ferrer A, Phillips MA. Distinct metabolic pathways drive monoterpenoid biosynthesis in a natural population of Pelargonium graveolens. J Exp Bot. 2020; 71(1):258-271.

Cavar S, Vidic D, Maksimovic M. Chemical composition and antioxidant activity of essential oil and aqueous extract of Pelargonium graveolens L'Her. Planta Medica, 2010; 76(12):P132.

Ram M, Singh R, Naqvi AA, Kumar S. Effect of planting time on the yield and quality of essential oil in geranium Pelargonium graveolens. J Hortic Sci. 1997; 72(5):807-810.

Rabelo P, Luz JMQ, Silva SM, Blank AF, Alves PB, Monteiro K, Fabre EG. Yield and Composition of Essential Oil of Pelargonium graveolens L. in Differents Forms of Cultivation and Fertilizations. Inter Symp on Med Plants and Nat Prod. 1098. 2013; 1-10.

Diovu E, Onah CO, Odo KE, Amaechina IN, Akpadolu UD, Nwodo JA, Chah CAT, Akupue UD, Nnadi CO. Sesquiterpene Lactone-Rich Extract of Tithonia diversifolia (Hemsley) A. Gray (Asteraceae) suppresses Trypanosoma brucei brucei in both. TJNPR 2022; 6(8):1268-1273.

Sandasi M, Kamatou GPP, Gavaghan C, Baranska M, Viljoena AM. A quality control method for geranium oil based on vibrational spectroscopy and chemometric data analysis. Vib. Spectrosc 2011; 57(2):242-247.

Wang M, Chittiboyina A, Avula B, Zhao J, Tabanca N, Wang YH, Weerasooriya A, Khan IA. Analytical investigation of geranium oils from Pelargonium graveolenes. Planta Medica 2012; 78(11):PJ71.

Ali O and Hassni N. Variations of the Chemical Constituents and Pharmacological Activities of Pelargonium graveolens Essential Oil from Three Regions in Palestine. An-Najah National University 2021:1-102.

Džamić AM, Soković MD, Ristić MS, Grujić SM, Mileski KS, Marin PD. Chemical composition, antifungal and antioxidant activity of Pelargonium graveolens essential oil. J Appl Pharm Sci. 2014; 4(03):001-005.

Peixoto JdC, Soković MD, Ristić MS, Grujić SM, Mileski KS, Marin PD. Flavonoids from Brazilian Cerrado: Biosynthesis, Chemical and Biological Profile. Molecules 2019; 24(16):1-14.

Nabavi SM, Šamec D, Tomczyk M, Milella L, Russo D, Habtemariam S, Suntar I, Rastrelli L, Daglia M, Xiao J, Giampieri F, Battino M, Sobarzo-Sanchez E, Nabavi SF, Yousefi B, Jeandet P, Xu S, Shirooie S. Flavonoid biosynthetic pathways in plants: Versatile targets for metabolic engineering. Biotechnol. Adv 2020; 38:107316.

Prasad D, Bist S, Tewari A, Singh U, Singh A. The role of phenolic compounds in disease resistance in geranium. Arch. Phytopathol. Pflanzenschutz 2010; 43(7):615-623.

Hashemabadi D, Aboksari HA, Sedaghathoor S, Kaviani B. Geranium (Pelargonium graveolens) extract and mechanical treatment improve water relation, enzyme activity and longevity of cut chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) flowers. Acta Sci Pol Hortorum Cultus. 2016; 15(5):185-203.

Al-Sayed E, Martiskainen O, Seif el-Din SH, Sabra AA, Hammam OA, Naglaa M. El-Lakkany, Protective effect of Pelargonium graveolens against carbon tetrachloride-induced hepatotoxicity in mice and characterization of its bioactive constituents by HPLC–PDA–ESI–MS/MS analysis. Med Chem Res. 2015; 24(4):1438-1448.

Androutsopoulou C, Christopoulou S, Hahalis P, Kotsalou C, Lamari F, Vantarakis A. Evaluation of essential oils and extracts of rose geranium and rose petals as natural preservatives in terms of toxicity, antimicrobial, and antiviral activity. Pathogens. 2021; 10(4):1-16.

Sofic E, Janicijevic AC, Salihovic M, Tahirovic I, Kroyer G. Screening of medicinal plant extracts for quercetin-3rutinoside (rutin) in Bosnia and Herzegovina. Med Plants Int J Phytomed. 2010; 2(2):97-102.

Williams CA, Newman M, Gibby M. The application of leaf phenolic evidence for systematic studies within the genus Pelargonium (Geraniaceae). Biochem Syst Ecol. 2000; 28(2):119-132.

Khan MK and Dangles O. A comprehensive review on flavanones, the major citrus polyphenols. J. Food Compos Anal. 2014; 33(1):85-104.

Jiang N, Doseff AI, Grotewold E. Flavones: from biosynthesis to health benefits. Plants. 2016; 5(2):1-27.

Omar HS, Elsayed TR, Reyad NA, Shamkh IM, Sedeek MS. Gene‐targeted molecular phylogeny, phytochemical analysis, antibacterial and antifungal activities of some medicinal plant species cultivated in Egypt. Phytochem Anal. 2021; 32(5):724- 739.

Saubade F, Pilkington L, Christopher ML, Gomes LC, McClements J, Peeters M, El Mohtadi M, Mergulhão FJ, Whitehead KA. Principal Component Analysis to Determine the Surface Properties That Influence the Self-Cleaning Action of Hydrophobic Plant Leaves. Langmuir, 2021; 37(27):8177- 8189.

Salem MA, Radwan RA, Mostafa ES, Alseekh S, Fernie AR, Ezzat SM. Using an UPLC/MS-based untargeted metabolomics approach for assessing the antioxidant capacity and anti-aging potential of selected herbs. RSC Advances. 2020; 10(52):31511-31524.

Hammerbacher A, Paetz C, Wright LP, Fischer TC, Bohlmann J, Davis AJ, Fenning TM, Gershenzon J, Schmidt A. Flavan-3- ols in Norway spruce: biosynthesis, accumulation, and function in response to attack by the bark beetle-associated fungus Ceratocystis polonica. Plant Physiol. 2014; 164(4):2107-2122.

Neagu AF, Costea T, Nencu I, Dutu LE, Popescu ML, Olaru OT, Gird CE. Obtaining and characterization of a selective Pelargonium graveolens l’Hér. dry extract with potential therapeutic activity in metabolic diseases. Farmacia. 2018; 66:592-596.

Irmisch S, Ruebsam H, Jancsik S, Yuen MMS, Madilao LL, Bohlmann J. Flavonol biosynthesis genes and their use in engineering the plant antidiabetic metabolite montbretin A. Plant physiol., 2019; 180(3):1277-1290.

Panche AN, Diwan AD, Chandra SR, Flavonoids: an overview. J Nutr Sci. 2016; 5:1-15.

Checkouri E, Reignier F, Robert-Da Silva C, Meilhac O. Evaluation of Polyphenol Content and Antioxidant Capacity of Aqueous Extracts from Eight Medicinal Plants from Reunion Island: Protection against Oxidative Stress in Red Blood Cells and Preadipocytes. Antioxidants. 2020; 9(10):1-14.

Wollenweber E, Dörr M, Christ M. Flavonoid aglycones from the leaf and stem exudates of some Geraniaceae species. Nat Prod Commun. 2011; 6(1):1-3.

Rashmi HB and Negi PS. Phenolic acids from vegetables: A review on processing stability and health benefits. Food Res Inter. 2020; 136:109298.

Kumar N and Goel N. Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnol Rep. 2019; 24:e0370.

Goleniowski M, Bonfill M, Cusido R, Palazón J. Phenolic acids. Natural products 2013; 3:1951-1973.

Fouly K, El-Tanbouly N, El-Hefnawy H, Rasheed D, Mohamadin A, Mariee A. In vitro and in vivo antioxidant activities of certain Egyptian plant extracts. MJPS 2004:1-7.

Schofield P, Mbugua D, Pell A. Analysis of condensed tannins: a review. Anim Feed Sci Technol. 2001; 91(1-2):21-40.

Bourgaud F, Hehn A, Larbat R, Doerpere S, Kellner S, Matern U. Biosynthesis of coumarins in plants: a major pathway still to be unravelled for cytochrome P450 enzymes. Phytochem Rev. 2006; 5(2):293-308.

Dey P, Kundu A, Kumar A, Gupta M, Lee BM, Bhakta T, Dash S, Kim SH. Analysis of alkaloids (indole alkaloids, isoquinoline alkaloids, tropane alkaloids), Recent advances in natural products analysis 2020; 505-567.

Lu JJ, Bao JL, Chen XP, Hwang M, Wang YT. Alkaloids isolated from natural herbs as the anticancer agents. eCAM, 2012; 1-12.

Arassu RT and Nambikkairaj B. Pelargonium graveolens plant leaf essential oil mediated green synthesis of Silver Nano particles and its antifungal activity against human pathogenic fungi. J Pharm Phytochem. 2018; 7:1778-1784.

Bloomer RJ, Harvey CI, Farney TM, Bell ZW, Canale R. Cardiorespiratory/Metabolic Laboratory, Department of Health and Sport Sciences, University of Memphis, Memphis, TN , BS &Robert E. Canale , MS Effects of 1, 3-dimethylamylamine and caffeine alone or in combination on heart rate and blood pressure in healthy men and women. Physician Sportsmed. 2011; 39(3):111-120.

Pawar RS, Tamta H, Ma J, Krynitsky JA, Grundel E, Wamer WG, Rader JI. Updates on chemical and biological research on botanical ingredients in dietary supplements. Anal Bioanal Chem. 2013; 405(13):4373-4384.

Austin KG, Travis J, Pace G, Lieberman HR. Analysis of 1, 3 dimethylamylamine concentrations in Geraniaceae, geranium oil and dietary supplements. Drug Test Anal. 2014; 6(7-8):797- 804.

Mustafavi SH, Naghdi-Badi H, Sękara A, Mehrafarin A, Janda T, Ghorbanpour M, Rafiee H. Polyamines and their possible mechanisms involved in plant physiological processes and elicitation of secondary metabolites. Acta Physiol. Plant. 2018; 40(6):1-19.

Marinkovic J, Markovic M, Nikolic B, Soldatovic I, Ivanov M, Ciric S, Sokovic M, Markovic D. Antibacterial and Antibiofilm Potential of Leptospermum petersonii FM Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates. J. Essent. Oil-Bear Plants 2021; 24(2):304-316.

Atailia I and Djahoudi A. Chemical composition and antibacterial activity of geranium essential oil (Pelargonium graveolens L’Hér.) cultivated in Algeria. Phétothérapie. 2015; 13:156-162.

Bigos M, Wasiela M, Kalemba D, Sienkiewicz M. Antimicrobial activity of geranium oil against clinical strains of Staphylococcus aureus. Mole. 2012; 17(9):10276-10291.

Abd El-Kareem SM, Rabbih M M, Elansary HO, Al-Mana F. Mass spectral fragmentation of Pelargonium graveolens essential oil using GC–MS semi-empirical calculations and biological potential. Processes 2020; 8(2):1-19.

Bamidele O. Phytoconstituent optimization by response surface methodology and pharmaceutical activities of Pelargonium graveolens LʼHér acetone extract. Int J Green Pharm. 2018; 12(3):1-13.

El Aanachi S, Gali L, Nacer SN, Bensouici C, Dari K, Aassila H. Phenolic contents and in vitro investigation of the antioxidant, enzyme inhibitory, photoprotective, and antimicrobial effects of the organic extracts of Pelargonium graveolens growing in Morocco. Biocatal Agric Biotechnol. 2020; 29:101819.

Ibrahim MA, Sallem OW, Abdelhassib MR, Eldahshan OA. Potentiation of anti-Helicobacter pylori activity of clarithromycin by Pelargonium graveolens oil. Arab J Gastroenterol. 2021; 22(3):224-228.

Breijyeh Z, Jubeh B, Karaman R. Resistance of gram-negative bacteria to current antibacterial agents and approaches to resolve it. Molecules 2020; 25(6):1340.

Setzer WN. Essential oils as complementary and alternative medicines for the treatment of influenza. Am J Essent Oils Nat Prod. 2016; 4(4):16-22.

Senthil K, Gokila VM, Wang CS, Chen CC, Chen YC, Lu LP, Huang CH, Lai CS, Wang SY. Geranium and lemon essential oils and their active compounds downregulate angiotensin- converting enzyme 2 (ACE2), a SARS-CoV-2 spike receptor- binding domain, in epithelial cells. Plants. 2020; 9(6):770.

Ralambondrainy M, Belarbi E, Viranaicken W, Baranauskienė R, Venskutonis PR, Desprès P, Roques P, Kalamouni C, Sélambarom J. In vitro comparison of three common essential oils mosquito repellents as inhibitors of the Ross River virus. PloS one. 2018; 13(5):1-14.

Essid R, Hammami M, Gharbi D, Karkouch I, B-Hamouda T, Elkahoui S, Limam F, Tabbene O. Antifungal mechanism of the combination of Cinnamomum verum and Pelargonium graveolens essential oils with fluconazole against pathogenic Candida strains. Appl Microbiol Biotechnol. 2017; 101(18):6993-7006.

Misra A, Srivastava AK, Srivastava NK, Khan A. Se- acquisition and reactive oxygen species role in growth, photosynthesis, photosynthetic pigments, and biochemical changes in essential oil (s) monoterpene of Geranium (Pelargonium graveolens L. Her.'ex. Ait.). Am. -Eurasian J Sustain Agric. 2010:39-47.

Assane IM, Valladao GM, Pilarski F. Chemical composition, cytotoxicity and antimicrobial activity of selected plant‐derived essential oils against fish pathogens. Aquac Res. 2021; 52(2):793-809.

Dashamiri S, Ghaedi M, Naghiha R, Salehi A, Jannesar R. Antibacterial, anti fungal and E. coli DNA cleavage of Euphorbia prostrata and Pelargonium graveolens extract and their combination with novel nanoparticles. Braz J Pharm Sci. 2019; 54:1-8.

Vimalanathan S and Hudson J. Anti-influenza virus activity of essential oils and vapors. Am J Essent Oils Nat Prod. 2014; 2(1):47-53.

Sadiki FZ, El Idrissi M, Sbiti M. Antibacterial properties of the essential oil of Pelargonium Graveolens L'Hér. RHAZES 2019; 4(4):17-23.

Benelli G, Pavela R, Canale A, Cianfaglione K, Ciaschetti G, Conti F, Nicoletti M, Senthil-Nathan S, Mehlhorn H, Maggi F. Acute larvicidal toxicity of five essential oils (Pinus nigra, Hyssopus officinalis, Satureja montana, Aloysia citrodora and Pelargonium graveolens) against the filariasis vector Culex quinquefasciatus: Synergistic and antagonistic effects. Parasitol Int. 2017; 66(2):166-171.

Malik T, Pant S, Chauhan N, Lohani H. Potentiation of antimicrobial activity of ciprofloxacin by Pelargonium graveolens essential oil against selected uropathogens. Phytother Res. 2011; 25(8):1225-1228.

Amini M, Zayeri F, Salehi M. Trend analysis of cardiovascular disease mortality, incidence, and mortality-to-incidence ratio: results from global burden of disease study 2017. BMC Public Health. 2021; 21(1):1-12.

Yücel D and Yücel E. Plants used in complementary medicine in the treatment of cardiovascular diseases in Turkey. JABS. 2020; 14(1):73-85.

Milutinović A, Šuput D, Zorc-Pleskovič R. Pathogenesis of atherosclerosis in the tunica intima, media, and adventitia of coronary arteries: An updated review. Bosn J Basic Med Sci. 2020; 20(1):1-10.

Juárez Z, Bach H, S-Arreola E, Bach H, Hernández LR. Protective antifungal activity of essential oils extracted from Buddleja perfoliata and Pelargonium graveolens against fungi isolated from stored grains. J Appl Microbiol. 2016; 120(5):1264-1270.

Clebak KT and Dambro AB. Hyperlipidemia: An Evidence- based Review of Current Guidelines. Cureus. 2020; 12(3):1-6.

Cho SY, Jun H, Lee JH, Jia Y, Kim KH, Lee SJ. Linalool reduces the expression of 3-hydroxy-3-methylglutaryl CoA reductase via sterol regulatory element binding protein-2-and ubiquitin-dependent mechanisms. FEBS letters 2011; 585(20):3289-3296.

Galle M, Crespo R, Kladniew BR, Villegas SM, Polo M, de Bravo MG. Suppression by geraniol of the growth of A549 human lung adenocarcinoma cells and inhibition of the mevalonate pathway in culture and in vivo: potential use in cancer chemotherapy. Nutr Cancer. 2014; 66(5):888-895.

Marmouzi I, Karym EM, Alami R, El Jemli M, Kharbach M, Mamouch F, Attar A, Faridi B, Cherrah Y, Faouzi MEA. Modulatory effect of Syzygium aromaticum and Pelargonium graveolens on oxidative and sodium nitroprusside stress and inflammation. OPEM 2019; 19(2):201-210

Pereira I, Severino P, Santos AC, Silva AM, Souto EB, Linalool bioactive properties and potential applicability in drug delivery systems. Colloids Surf B Biointerfaces. 2018; 171:566-578.

Kshirsagar RP, Kothamasu MV, Patil MA, Reddy GB, Kumar BD, Diwan PV. Geranium oil ameliorates endothelial dysfunction in high fat high sucrose diet induced metabolic complications in rats. J Funct Foods. 2015; 15:284-293.

Association AD. Diagnosis and classification of diabetes mellitus. Diabetes care 2004; 27(1):5-10.

Kasabri V, Afifi FU, Hamdan I. Evaluation of the acute antihyperglycemic effects of four selected indigenous plants from Jordan used in traditional medicine. Pharm Biol. 2011 49(7):687-695.

Rahman ANA, Mohamed AR, Mohammed HH, Elseddawy NM, Salem GA, El-Ghareeb WR, The ameliorative role of geranium (Pelargonium graveolens) essential oil against hepato-renal toxicity, immunosuppression, and oxidative stress of profenofos in common carp, Cyprinus carpio (L.). Aquaculture 2020; 517:774-777.

Giongo JL, Vaucher RA, Sagrillo MR, Santos RCV, Duarte MMF, Rech VC, Lopes LQS, da Cruz IB, Tatsch E, Moresco RN, Gomes P, Luchese C, Steppe M. Anti-inflammatory effect of geranium nanoemulsion macrophages induced with soluble protein of Candida albicans. Microb Pathog. 2017; 110:694- 702.

El-Garawani I, Sobhy EH, Nafie H, Samar E. Foeniculum Vulgare and Pelargonium graveolens essential oil mixture triggers the cell cycle arrest and apoptosis in MCF-7 cells. AAMCE 2019;19(9):1103-1113.

Cheng Y, Dai C, Zhang J. SIRT3-SOD2-ROS pathway is involved in linalool-induced glioma cell apoptotic death. Acta Biochim. Pol. 2017; 64(2):343-350.

Jana S, Patra K, Sarkar S, Jana J, Mukherjee G, Bhattacharjee S, Mandal DP. Antitumorigenic potential of linalool is accompanied by modulation of oxidative stress: an in vivo study in sarcoma-180 solid tumor model. Nutr Cancer. 2014; 66(5):835-848.

Elansary HO, Abdelgaleil SAM, Mahmoud EA, Yessoufou K, Elhindi K, Hendawy SE. Effective antioxidant, antimicrobial and anticancer activities of essential oils of horticultural aromatic crops in northern Egypt. BMC Complement Altern Med. 2018; 18(1):1-10.

Nikolić M, Marković T, Marković D, Calhelha R, Fernandes A, Ferreira I, Stojković D, Ćirić A, Glamočlija J, Soković M. Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute. J Serb Chem. 2021; 86(12):1291-1303.

Sun XB, Wang SM, Li T, Yang YQ. Anticancer activity of linalool terpenoid: apoptosis induction and cell cycle arrest in prostate cancer cells. TJPLS 2015; 14(4):619-625.

Zhao Y, Chen RBS, Yang Y. In vitro and in vivo efficacy studies of Lavender angustifolia essential oil and its active constituents on the proliferation of human prostate cancer. Integr Cancer Ther. 2017; 16(2):215-226.

Boukhatem M, Sudha T, Darwish NHE, Nada HG, Mousa SA. Rose-scented geranium essential oil from Algeria (Pelargonium graveolens L'Hérit.): Assessment of antioxidant, anti- inflammatory and anticancer properties against different metastatic cancer cell lines. Ann pharm. fr 2021; 80(3):383- 396.

Park ST, Chuang TF, Chao SH, Yang JH, Lin YC, Huang HY. Effects of Geranium Oil on Antioxidative Activity and Melanin Synthesis in Cultured Human Skin Melanoma Cell. J Invest Cosmetol. 2011; 7:249-255.

Ren P, Cheng L, Xu L. Frankincense, pine needle and geranium essential oils suppress tumor progression through the regulation of the AMPK/mTOR pathway in breast cancer. Oncol Rep. 2018; 39(1):129-137.

Sunmi L. Effect of Geranium Essential Oil on Melanin Synthesis in B16F10 Melanoma Cells. JKSC 2018; 8(3):407- 414.

Gunaseelan S, Balupillai A, Govindasamy K, Muthusamy G, Ramasamy K, Shanmugam M, Prasad NR. The preventive effect of linalool on acute and chronic UVB-mediated skin carcinogenesis in Swiss albino mice. Photochem Photobiol Sci. 2016; 15(7):851-860.

Paltiyan JC, Dumale JV, Divina CC. Phytochemical analysis, larvicidal activity and cytotoxic properties of malvarosa (Pelargonium graveolens) leaf extract. Int J Agric Technol. 2017; 13(7.3):2281-2295.

Kimura R. Beneficial effects of essential oils on adult T-cell leukemia. Japan J Aromatherapy 2015; 16(1):15-24.

Lalli J, Van ZRL, Van Vuuren SF, Viljoe AM. In vitro biological activities of South African Pelargonium (Geraniaceae) species. S Afr J Bot. 2008; 74(1):153-157.

Slima AB, Ali MB, Barkallah M, Traore AI, Boudawara T, Allouche N, Gdoura R. Antioxidant properties of Pelargonium graveolens L’Her essential oil on the reproductive damage induced by deltamethrin in mice as compared to alpha- tocopherol. Lipids Health Dis. 2013; 12(1):1-9.