Antioxidant Activity of Column Fractions and Caryatin Isolated from the Ethyl acetate Extract of Dioscorea hirtiflora tuber doi.org/10.26538/tjnpr/v4i7.4
Main Article Content
Abstract
The tubers of Dioscorea hirtifloraBenth. are widely used as food during famine and as medicine in South-western Nigeria. The aim of this study was to identify bioactive compound(s) from the tuber of Dioscorea hirtiflora. Bioactive constituents from the ethyl acetate extract and fractions of the tubers were identified using gas chromatography-mass spectrometry (GC-MS), and by reverse phase-high performance liquid chromatography (RP-HPLC). The extract was subjected to column chromatography (CC) and preparative thin layer chromatography (PTLC) for the isolation of constituent(s). Fractions from the column chromatography of the extract as well as
an isolated compound from the sub-fraction of the extract were evaluated for their antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The GC-MS of the derivatized ethyl acetate extract showed the presence of phenolics (benzoic acid, 4-hydroxybenzoic acid and 3,4-dihydroxybenzoic acid) while the RP-HPLC confirmed the presence of gallic acid, protocatechuic acid, catechin, isovanillic acid and quercetin in the fractions. CC and PTLC of the extract led to the isolation of compound I-24characterised as 3,5- dimethoxy-7, 3´, 4´-trihydroxy flavone (caryatin) by a combination of nuclear magnetic
resonance (NMR) spectroscopy and mass spectrometry (MS), and by comparison of spectra data with literature. Fraction I and sub-fraction I-2 had the highest antioxidant activity with IC50 values of 2.11 ± 0.09 and 4.67 ± 0.06 µg/mL, respectively,while the isolated compound had IC50 value of 49.4 ± 2.18 µg/mL.The effect of these compounds as antioxidant could give credence to their traditional use as food and medicine.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
References
Wilkin P. Dioscoreaceae of South-Central Africa. Kew Bull. 2001; 56:361.
Iwu MM, Okunji CO, Ohiaeri GO, Akah P, Corley D. Hypoglycaemic activity of dioscoretine from tubers of Dioscoreadumetorum. Planta Med. 1990; 56:264-267.
Komori T. Glycosides from Dioscoreabulbifera. Toxicon. 1997; 35(10):1531-1535.
Li SS, Deng JZ, Zhao SX. Steroids from tubers of Dioscoreabulbifera. J Plant Resour Environ. 1999; 8:61–62.
Liu H, Chou GX, Wu T, Guo YL, Wang SC, Wang CH, Wang ZT. Steroidal Sapogenins and Glycosides from the Rhizomes of Dioscoreabulbifera. J Nat Prod. 2009; 72:1964–1968.
Wang G, Liu JS, Lin BB, Wang GK, Liu JK. Two new furanoidnorditerpenes from Dioscoreabulbifera. Chem Pharm Bull. 2009; 57:625–627.
Gao H, Hou B, Kuroyanagi M, Wu L. Constituents from anti-tumour promoting active part of Dioscoreabulbifera in JB6 mouse epidermal cells. Asian J Trad Med. 2007; 3:104- 109.
Adeniran AA and Sonibare MA. In vitro antioxidant activity, brine shrimp lethality and assessment of bioactive constituents of three wild Dioscorea species. JFood MeasCharact. 2017; 11:685-695.
Adeniran AA, Sonibare MA, Rajacharya GH, Kumar S. Assessment of genetic fidelity of Dioscoreabulbifera L. and DioscoreahirtifloraBenth. and medicinal bioactivity produced from the induced tuberous root. Plant Cell Tiss Org. 2018; 132:343-357.
Fiehn O, Kopka J, Willmitzer L. Metabolite profiling for plant functional genomics. Nat. Biotechnol. 2000; 18(11):1157-1161.
Mitova M, Taskova R, Popov S, Berger RG, Krings U,Handjieva N. GC/MS analysis of some bioactive constituents from Carthamuslanatus L. Zeitschrift fur Naturforsch C. 2003; 58:697-703.
Dey P and Chaudhuri KT. Phytochemical characterization of Dioscoreaalata leaf and stem by silylation followed by GC-MS analysis. Food Chem. 2016; 1:1-6.
Seal T. Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves, Sonchusarvensis and Oenanthelinearis of North-Eastern region in India J Pharm Sci. 2016; 1:157–166.
Ahmadi-Sakha S, Sharifi M, Niknam V. Bioproduction of phenylethanoid glycosides by plant cell culture of ScrophulariastriataBoiss.: from shake-flasks to bioreactor. Plant Cell Tiss Org. 2016; 124:275–281.
Bursal E and Gülçin I. Polyphenol content and in vitro antioxidant activities of lyophilized aqueous extract of kiwifruit (Actinidiadeliciosa). Food Res. 2011; 44(5):1482– 1489.
Dey P and Chaudhuri TK. Phytochemical characterization of DioscoreaAlata leaf and stem by silylation followed by GC-MS analysis. J Food Biochem. 2015; 40(4):630-635.
Dey P, Ray S, Chaudhuri TK. Immunomodulatory activities and phytochemical characterization of the methanolic extract of Dioscoreaalata aerial tuber. J Funct Foods 2016; 23:315-328.
Agrawal PK. Carbon-13 NMR of flavonoids. Elsevier, Amsterdam, Nertherlands. Chapter 6. 1989. 283-364 p.
Mabry TJ, Markham KR, Thomas MB. The systematic identification of flavonoids. Springer, Berlin. 1970. 24-35 p.
Abdallah HM, Salama MM, Abd-elrahman EH, El- Maraghy SA. Antidiabeticactivity of phenolic compounds from Pecan bark in streptozotocin-induced diabetic rats. PhytochemLett. 2011; 4:337-341.
Zhou W, Oh J, Li W, Kim DW, Lee SH, Na M. Phytochemical studies of Korean Endangered plants: A new flavone from Rhododendron brachycarpum G. Don. B. Korean Chem Soc. 2013; 34(8):2535-2538.
Gao HY, Kuroyanagi M, Wu LJ, Kawahara N, Yasuno T, Nakamura Y. Antitumour promoting constituents from Dioscoreabulbifera L. in JB6 mouse epidermal cells. Bio Pharm Bull. 2002; 25(12):1241–1243.
Suzuki H, Yamamoto S, Hirayama C, Takino T, Fujisawa K, Oda T. Depression UVB-induced skin stress and inhibition of intestinal tumour. Liver.1986; 6(1):35-44.
Jeon SE, Choi-Kwon S, Park KA, Lee HJ, Park MS, Lee JH. Treatment of viral hepatitis by (+)-catechin. PhotodermatolPhotoimmunolPhotomed. 2003; 19(1):235- 239.