Assessment of Microbial Contamination of Bacteria Isolates from Fermented Cassava (Gari) Sold at Samaru Market, Zaria, Kaduna State and Antibiotic Susceptibility Pattern of Isolated Bacteria
oi.org/10.26538/tjnpr/v4i9.15
Keywords:
Susceptibility testing, Antibiotics, Bacteria contamination, Fermented cassavaAbstract
Fermented cassava is a staple food popularly consumed in Nigeria. It is normally sold in open markets in basins, open sacks and wheel barrows. Contamination of this food product is a potential source of public health hazard more especially when the product is consumed raw or merely soaked in cold water. The aims of this study were to assess the microbial contamination of gari sold in Samaru market, Zaria, Kaduna State, Nigeria and determine the antibiotics susceptibility pattern of the bacteria isolated. Ten samples were collected from different sources and serially diluted. The diluted samples were plated on nutrient agar and MacConkey agar to
determine the total viable bacteria counts and coliform counts, respectively. Antibiotics susceptibility testing was carried out using Kirby Bauer agar diffusion method according to Clinical Laboratory Standard International.The average total bacterial count was 5.20 x 104 CFU/g while the average coliform count was 2.62 x 104 CFU/g. The following bacterial contaminants were isolated from the samples: Staphylococcus aureus 15 (44.1%), Bacillus spp. 7 (20.6%), Escherichia coli 7 (20.6%), Pseudomonas spp. 3 (8.8%) and Salmonella spp. 2 (5.9%). The isolates were highly susceptible to gentamicin and ciprofloxacin, but highly resistant to beta-lactam antibiotics used in the study. The presence of antibiotics resistant bacterial strains in gari sold in the market is a potential source of transmission of antibiotic-resistant strains in the community which can lead to treatment failure of infections caused by such organisms.
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