Phytochemical, Antioxidant and Anti-Inflammatory Characterization of Leaves and Bark of Mimusops coriacea (A.DC) Miq from Ecuador
oi.org/10.26538/tjnpr/v4i9.14
Keywords:
Phytochemical, Mimusops coriacea, Leaves, Barks, Anti-inflammatory activity, Antioxidant activityAbstract
Mimusops coriacea (A.DC) belonging to the family Sapotacea is a tree that grows widely in Ecuador. It is traditionally used as an analgesic and anti-inflammatory medicine. There is no phytochemical information on the leaves and bark of the plant, or pharmacological studies that justifies its traditional use. The objective of this study was to determine the chemical composition, antioxidant and anti-inflammatory activity of hydroalcoholic extracts of the leaves and bark of M. coriacea. Extracts were obtained by maceration from leaves and bark, using 80% hydroalcohol as solvent. Total phenols were quantified by Folin-Ciocalteu, total flavonoids by the colorimetric method of aluminum chloride and an analysis was performed by LC-MS. The antioxidant capacity by FRAP, DPPH and ABTS assays and anti-inflammatory activity using carrageenan-induced paw edema model in rats were evaluated. The results revealed a significant difference in the content of phenols and flavonoids, being higher for the leaf extract. Two glycosylated flavonoids (myricetin-3-O-α-L-rhamnoside and myricetin-3-O-glucoside) and two triterpenic saponins derived from protobasic acid were identified by LC-MS. The two extracts showed a high ferro-reducing capacity and antiradical activity, with the leaf extract being the most active (IC50 = 4.58 μg/mL, DPPH and 197.00 μg/mL, ABTS). Both the extract from the leaves and the bark showed an anti-inflammatory effect, somewhat higher than the extract from the leaves. The results provide the first findings on the chemical and biological study of the
leaves and bark of M. coriacea that grow in Ecuador, which justifies the traditional use of the species as anti-inflammatory.
References
Silva V, Falco V, Dias MI, Barros L, Silva A, Capita R, Alonso-Calleja C, Amaral JS, Igrejas G, Ferreira ICFR, Poeta P. Evaluation of the phenolic profile of Castanea sativa Mill. by-products and their antioxidant and antimicrobial activity against multiresistant bacteria. Antioxid. 2020; 9(87):1-14.
Chukwu EC, Osuocha KU, Iwueke AV. Phytochemical profiling, body weight effect and antihypercholesterolemia potentials of Cnidoscolus aconitifolius leaf extracts in male albino rat. J Pharmacogn Phytother. 2020; 12(2):19-27.
Allegra M. Antioxidant and Anti-Inflammatory Properties of Plants Extract. Antioxid. 2019; 8:549-553.
Borquaye LS, Laryea MK, Gasu EN, Boateng MA, Baffour PK , Kyeremateng A, Doh G. Anti-inflammatory and antioxidant activities of extracts of Reissantia indica, Cissus cornifolia and Grosseria vignei. Cogent Bio. 2020; 6:1785755.
Konaré MA, Diarra N, Cissé Ch, Traoré DAK, Togola I, Kassogué A, Sanogo R, Outtara AS. Evaluation of the biological activities of leaf and bark extracts of Ficus platiphylla Delile, a medicinal plant used in Mali. J Med Plants Res. 2020; 14(3):118-128.
Database of tropical plants. Ken Fern. tropical.theferns.info. 2019. [online]. Available from: tropical.theferns.info/viewtropical.php?id=Mimusops+coria cea.
Baliga MS, Pai RJ, Bhat HP, Palatty PL, Boloor R. Chemistry and medicinal properties of the Bakul (Mimusops elengi Linn): a review. Food Res Int. 2011; 44(7):1823- 1829.
Semenya S, Potgieter M, Erasmus L. Ethnobotanical survey of medicinal plants used by Bapedi healers to treat diabetes mellitus in the Limpopo Province, South Africa. J Ethnopharmacol. 2012; 141(1):440-445.
Erazo N. Compendio de plantas medicinales del Ecuador. Riobamba: Escuela Superior Politécnica de Chimborazo; 2010. Aviable from:
https://www.google.com/search?q=Erazo+N.+2010.
Rocero C, Iturralde G, Zambrano R, Vallardo V. Ampliación del área nacional de recreación Los Samanes. Ministerio de Ambiente. Ecuador. 2010:1-94
Bustamante K, Santos-Ordóñez E, Miranda M, Pacheco R, Gutiérrez Y, Scull R. Morphological and molecular barcode analysis of the medicinal tree Mimusops coriacea (A.DC.) Miq. collected in Ecuador. Peer J. 2019; 7:e7789.
Miranda MM and Cuéllar AC. Manual de prácticas de laboratorio. Farmacognosia y productos naturales. Universidad de la Habana. Ciudad Habana, Cuba; 2000: 56-57 p.
Chlopicka J, Pasko P, Gorinstein S, Jedryas A, Zagrodzki P. Total phenolic and total flavonoid content, antioxidant activity and sensory evaluation of pseudocereal breads. LWT- Food Sci Technol. 2012; 46:548-555.
Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002; 10(3):178- 182.
Pourmorad F, Hosseinimerhr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol. 2006; 5(11):1142-1145.
Benzie IFF and Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal Biochem. 1996; 239:70-76.
Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm. Wiss. Technol. 1995; 22:25-30.
Kedare SB and Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011; 48(4):412-422.
Agudo LM. Técnicas para la determinación de compuestos antioxidante en alimentos. Autodidacta. Revista de la Educación en Extremadura 2010; 27-34 p.
Arnao MB, Cano A, Acosta M. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem Barking 2001; 73(2):239-244.
Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rat as an assay for antiinflammatory drugs. Proc Soc Exp Biol Med Soc Exp Biol Med N Y N. 1962; 111:544-547.
González GMC, Ospina GLF, Rincón VJ. Actividad antiinflamatoria de extractos y fracciones de Myrcianthes leucoxila, Calea prunifolia, Curatella americana y Physalis peruviana en los modelos edema auricular por TPA, edema plantar por carragenina y artritis inducida por colágeno. Biosalud. 2011; 10(1):9-18.
Balamurugan K, Sakthidevi G, Mohan VR. Antiinflammatory activity of leaf of Melastoma malabathricum L. (Melastomataceae). Int J Res Ayurv Pharm. 2012; 3(6):801-802.
The World Medical Association. Declaración de la AMM sobre el Uso de Animales en la Investigación Biomédica. 2016. [WMA Statement on the Use of Animals in Biomedical Research]. [cited 2019 march 2] Available from: https://www.wma.net/es/policies-post/declaracion-dela-amm-sobre-el-uso-de-animales-en-la-investigacionbiomedica/.
Moustafa HB, Amal MK, Mohamed RE, Eman GH. A Review on Phenolic Compounds from Family Sapotaceae. J Pharmacogn Phytochem. 2016; 5(2):280-287.
Oladunni BF, Omotayo TAA, Sabiu S, Ayokun-nun AA, Palanisamy PC, Idowu KM, Adedeji AA. Pharmacognosy: Importance and drawbacks. In: Pharmacognosy - Medicinal Plants. IntechOpen; 2019. 1-18 p.
Zlati´c N, Jakovljevi´c D, Stankovi´c M. Temporal, plant part, and interpopulation variability of secondary metabolites and antioxidant activity of Inula helenium L. Plant 2019; 8(179):10 pages.
Mondal S and Rahaman ST. Flavonoids: A vital resource in healthcare and medicine. Pharm Pharmacol Int J. 2020; 8(2):91-104.
Thuan NH, Pandey RP, Thuy TTT, Park JW, Sohng JK. Improvement of regio-specific production of myricetin-3- O-α-L-rhamnoside in engineered Escherichia coli. Appl Biochem Biotechnol. 2013; 171(8):1956-1967.
Mendes RF, Pena FMJ, Hartvig CM, Rossi M, Furlan CM. Acylated Flavonoid Glycosides are the Main Pigments that Determine the Flower Colour of the Brazilian Native Tree Tibouchina pulchra (Cham.) Cogn Mol. 2019; 24:718.
Eskander J, Lavaud C, Abdel-khalik SM, Soliman HS, Mahmoud II, Long C. Saponins from the Leaves of Mimusops laurifolia. J Nat Prod. 2005; 68(6):832-841.
Gami B, Pathak S, Parabia M. Ethnobotanical, phytochemical and pharmacological review of Mimusops elengi Linn. Asian Pac J Trop Biomed. 2012; 2(9):743-748.
Pudziuvelyte L, Liaudanskas M, Jekabsone A, Sadauskiene I, Bernatoniene J. Elsholtzia ciliata (Thunb.) Hyl. Extracts from different plant parts: Phenolic composition, antioxidant, and anti-inflammatory activities. Mol. 2020; 25(5):1153-1162.
Agu K, Okolie NP, Najdia E, Falodun A. In vitro anticancer assessments of Annona muricata fractions and in vitro antioxidant profile of fractions and isolated acetogenin (15-acetyl guanacone). J Cancer Res Prac. 2018; 5:53-66.
Egharevba E, Chukwuemeke-Nwani P, Eboh U, Okoye E, Olapeju Bolanle I, Oseghale IO, Imieje VO, Erharuyi O, Falodun A. Antioxidant and Hypoglycaemic Potentials of the Leaf Extracts of Stachytarphyta jamaicensis (Verbenaceae). Trop J Nat Prod Res. 2019; 3(5):170-174.
Ogbeide OK, Okhomina OK, Omoregie IG, Unuigbe CA, Ighodaro A, Akhigbe IU, Iheanacho CM, Akubuiro PC, Solomon A, Irabor EEI, Owolabi BJ, Falodun A. Antimalarial, ferric reducing Antioxidant Power and Elemental Analysis of Caesalpinia pulcherrima leaf extract.
J Chem Soc Nig. 2020; 45(4):704 -711.
Afsar T, Razak S, Shabbir M, Rashid KM. Antioxidant activity of polyphenolic compounds isolated from ethyl‑acetate fraction of Acacia hydaspica R. Parker. Chem Centr J. 2018; 12:5.
Uddin MM, Mostari F, Yeasmin S, Jalil MA, Akter N, Amin R, Islam B. Evaluation of in vitro free radical scavenging and antioxidant activities of Mimusops elengi (Bokul) leaf extract. Int J Innov Pharm Sci Res. 2018; 6(09):19-29.
Dutta S and Ray S. Comparative assessment of total phenolic content and in vitro antioxidant activities of bark and leaf methanolic extracts of Manilkara hexandra (Roxb.) Dubard. J King Saud Univ Sci. 2020; 32:643-647.
Mital K and Sumitra C. Evaluation of antioxidant and antimicrobial properties of Manilkara zapota L. (chiku) leaves by sequential soxhlet extraction method. Asian Pac J Trop Biomed. 2012; 2(3):S1526-S1533.
Fernández RGA, Cruzado LM, Bonilla RPE, Ramírez CFJM, Toche TA, Curay CVL. Identificación de metabolitos secundarios y efecto antiinflamatorio del extracto etanólico de hojas de Chromolaena leptocephala (DC) R.M. King & H. Rob. “chilca negra”. Rev Per Med
Integrat. 2017; 2(3):779-784.
Purnima A, Koti BC, Thippeswamy AHM, Jaji MS, Vishwantha SAH, Kurhe YV, Sadiq AJ. Antiinflammatory, Analgesic and Antipyretic Activities of Mimusops elengi Linn. Indian J Pharm Sci. 2010; 72(4):480-485.
Babiaka SB, Nia R, Abuga KO, Mbah JA, Nziko VN, Paper DH, . Antioxidant potential of flavonoid glycosides from Manniophyton fulvum Müll. (Euphorbiaceae): Identification and molecular modeling. Sci Afr. 2020; 8(e00423):1-7.
Alemu A, Tamiru W, Nedi T, Shibeshi W. Analgesic and anti-inflammatory effects of 80% methanol extract of Leonotiso cymifolia (Burm.f.) iwarsson leaves in rodent models. Hindawi. Evid-Based Compl Altern Med. 2018:1- 88.
Ginwala R, Bhavsar R, Chigbu DeGaulle I, Jain P, Khan ZK. Potential role of flavonoids in treating chronic inflammatory diseases with a special focus on the antiinflammatory activity of apigenin. Antioxid. 2019; 8(35):30.
Chen S, Rong Y, Liu M, Cheng S, Liu X, Li X, Yu Y, Yang G, Yang X. Analgesic effects of triterpenoid saponins from Stauntonia chinensis via selective Increase in Inhibitory synaptic response in mouse cortical neurons. Front Pharmacol. 2018; 9:12 pages.
El Hazzam K, Hafsa J, Sobeh M, Mhada M, Taourirte M, EL Kacimi K, Yasri A. An Insight into Saponins from Quinoa (Chenopodium quinoa Willd): A Review. Molecules 2020; 25, 1059.
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