Anti-hypertensive Evaluation of n-Hexane and Hydro-ethanol Fruit Peel Extracts of Persea americana Mill. (Lauraceae) in albino rats
doi.org/10.26538/tjnpr/v4i5.1
Keywords:
Persea americana, Phytochemical Investigation,, Antihypertensive,, n-Hexane,, Hydro-ethanolic Extracts.Abstract
The fruits of Persea americana are consumed in Nigeria and are reported to have antihypertensive properties in folklore use. Hypertension is currently one of the major risk factors for cardiovascular diseases that lead to high morbidity and mortality globally. The present study was designed to evaluate the antihypertensive effects of n-Hexane and hydroethanol extracts of the fruit peel of P. americana on high-salt diet-induced hypertension rats. The antihypertensive investigation of the extracts at different doses (100, 200, and 400 mg/kg) showed a remarkable dose-dependent effect. At 100 mg/kg, the systolic and diastolic blood pressure (SBP/DBP) for n-hexane and hydro-ethanol extracts were 124.28/103.83 mmHg and 122.72/104.78 mmHg, with pulse pressure (PP) of 20.45 and 17.94 mmHg, respectively. At 200mg/kg, the SBP/DBP were 125.01/112.54 mmHg and 119.92/110.69 mmHg with PP of 12.47 and 9.23 mmHg, respectively. At 400 mg/kg, the SBP/DBP were 117.66/98.8 mmHg and 135.88/121.94 mmHg, with PP of 8.86 and 13.94 mmHg, respectively. The standard drug nifedipine gave SBP/DBP of 105.17/87.72 mmHg with PP of 17.45 mmHg, while other standards gave higher SBP/DBP values; HCT (138.28/126.81 mmHg), telmisartan (174.94/141.29 mmHg) and enalapril (128.99/115.08 mmHg). The results from the highest dose of the n-Hexane extract (400 mg/kg) compared favourably with that of the standard (nifedipine) which shows that the extract might be acting through a similar mechanism as that of nifedipine. The reduction of BP in this study could be due to the presence of identified secondary metabolites in the plant.
References
Owolabi MA, Jaja SI, Coker HA. Vasorelaxant action of aqueous extract of the leaves of Persea americana on isolated thoracic rat aorta. Fitoterapia 2005; 76:567–573.
Noorul H, Mijahid M, Badruddeen KM, Vartika S, Nesar A, Zafar K, Zohrameena S. Physico-Phytochemical analysis and estimation of total phenolic, flavonoids and proanthocyanidin contents of Persea americana (Avocado) seed extract. World J Pharm Sci. 2017; 5(4):70-77.
Nayak BS, Raju SS, Chalapathi, RAV. Wound healing activity of Persea americana (avocado) fruit: a preclinical study on rats. J Wound Care. 2008; 17:123–126.
Yasir M, Das S, Kharya MD. The Phytochemical and Pharmacological profile of Persea americana Mill.Pharmacogn Rev. 2010; 4(7):77-84.
Dzeufiet PDD, Mogueo A, Bilande DC, Aboubakar BO, Tedong L, Dimo T, Kamtchouing P. Antihypertensive potential of the aqueous extract which combine leaf of Persea americana Mill. (Lauraceae), stem and leaf of Cymbopogon citratus (D.C) Sapt. (Poaceae), fruits of Citrus
medical L. (Rutaceae) as well as honey in ethanol and sucrose experimental model. BMC Compl Altern Med.2014; 14:507.
Kawagishi H, Fukumoto Y, Hatakeyama M, He P, Arimoto H, Matsuzawa T, Arimoto Y, Suganuma H, Inakuma T, Sugiyama K. Liver injury suppressing compounds from avocado (Persea americana). J Agric Food Chem. 2001; 49:2215–2221.
Lu QY, Arteaga JR, Zhang Q, Huerta S, Go V, Heber D. Inhibition of prostate cancer cell growth by an avocado extract: Role of lipid-soluble bioactive substances. J Nutr Biochem. 2005; 16:23–30.
Adeyemi OO, Okpo SO, Ogunti OO. Analgesic and antiinflammatory effects of the aqueous extract of leaves of
Persea americana Mill (Lauraceae). Fitoterapia. 2002;73:375–380.
Ojewole JA and Amabeoku GJ. Anticonvulsant effect of Persea americana Mill (Lauraceae) (Avocado) leaf aqueous extract in mice. Phytother Res. 2006; 20:696–700.
Brai BI, Odetola AA, Agom PU. Hypoglycemic and hypocholesterolemic potential of Persea americana leaf extracts. 2007; 10:356–360.
Ojewole JA, Kamadyaapa DR, Gondwe MM, Moodley K, Musabayane CT. Cardiovascular effects of Persea americana Mill (Lauraceae) (avocado) aqueous leaf extract in experimental animals. Cardiovasc J Afr. 2007; 18:69–76.
De Ribeiro A, Fiuza MMMR, Barros F, Gomes C, Trolin G. Acute antihypertensive effects in conscious rats produced by some medicinal plants used in the state of Sao Paulo. J Ethnopharmacol. 1986; 15:261–269.
Badyal DK, Lata H, Dadhich AP. Animal models of hypotensive and effect of drugs. India J Pharmacol. 2003;35:349-362.
Sofowora A. Medicinal Plants and Traditional Medicine in Africa. 3nd Ed. Spectrum Books Ltd, sunshine House, Ibadan, Nigeria. 2008. 130 p.
Evans WC. Trease and Evans Pharmacognosy, 15th Ed. Harcourt Publishers Ltd. China. 2009. 585 p.
Harborne JB. Photochemical Methods: A Guide to Modern Techniques of Plant Analysis 3rd Ed. Chapman A and Hall. London. 1998. 1-301 p.
Sofola OA, Knill A, Hainsworth R, Drinkhill M. Change in endothelial function in mesenteric arteries of Sprague‐Dawley rats fed at high salt diet. J Physiol. 2002; 543(1):255-260.
Balogun ME, Nwachukwu DC, Iyare EE, Besong EE, Obimma JN, Djobissie SF. Antihypertensive effect ofmethanolic extract from the leaves of Hibiscus Sabdariffa L. in rats. Der Pharm Lett 2016; 8(19):473-484.
Badyal DK, Lata H, Dadhichi.AP Animal models of hypertension and effect of drugs. Indian J Pharmcol. 2003;35:349-362.
Anon Video downloads from https//www.ncbi.nih.gov/pmc/articles/pmc2794298/bin/jove-27-1291.flv. Accessed 23/04/2020.
Naish J, Denise C, Combe S. Medical Sciences (2nd ed.). 2014. 562 p.
Lackland DT and Weber MA. Global burden of cardiovascular diseases and stroke: hypertension at the core. The Canadian J Cardiol, 2015; 31(5):569-571.
Xin-fang L, Chun-Yi NG, Kamsiah J. Animal models in cardiovascular research: Hypertension and Atherosclerosis. Biomed Res Int. 2015; 2015:1-11.
Reid JL, Millar JA, Struthers AD. Nifedipine-Studies on its mechanism of action and interaction with other circulatory control mechanism in man. Postgrad Med J. 1983; 59(2):98-103.
Judith MMD. Treating hypertension with calcium channel blockers. Healthline Affairs Team. 2018. Retrieved fromhttps://www.health/heart-disease/calcium channel blockers. Accessed 22/4/2020.
Sweet CS, Gaul SL, Reitz PM, Blaine EH, Ribeiro LT. Mechanism of action of enalapril in experimental hypertension and acute left ventricular failure. J Hypertens Supp. 1983; 1(1):53-63.
Linda LH and Khalid B. Hydrochlorothiazide. Stat pearls Publishing. 2019; 929 p.
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