Membrane Stabilization, Albumin Denaturation, Protease Inhibition, and Antioxidant Activity as Possible Mechanisms for the Anti-Inflammatory Effects of Flavonoid-Rich Extract of Peltophorum pterocarpum (DC.) K.Heyne (FREPP) Stem Bark doi.org/10.26538/tjnpr/v4i10.25

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Published: Oct 1, 2020
Keywords:
Flavonoid, Anti-inflammatory, Antioxidant, Stem bark, Peltophorum pterocarpum

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Osmund C. Enechi
Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria
Emmanuel S. Okeke
Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria
Nicodemus E. Nwankwo
Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria
John E. Nweze
Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria
Chukwuebuka P. Obilor
Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria
Christopher I. Okoye
Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria
Ogochukwu E. Awoh
Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria

Abstract

The ever increasing discovery of large number medicinal plants and the screening of their bioactivity to provide data that will help physicians and patients make wise decision before using them. The study evaluated the membrane stabilization, albumin denaturation, protease inhibition and antioxidant activities as possible mechanisms for the anti-inflammatory effects of the stem-bark flavonoid-rich extract of Peltophorum pterocarpum (FREPP). The antioxidant activities were assessed using the hydrogen peroxide, nitric oxide (NO) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays. The anti-inflammatory effect of FREPP was determined using the membrane stabilization model, albumin denaturation, protease inhibitor assay, while the total flavonoid was examined using the aluminium chloride colorimetric test. At 0.1, 0.4, 0.8 mg/mL, FREPP inhibited hypotonicity-induced haemolysis by 61.03, 76.84 and 83.84%, respectively. FREPP was effective in inhibiting albumin denaturation in a concentration-dependent manner with the highest inhibition of 69.89% at 50 µg/mL. Protease activity was significantly (P < 0.05) reduced at all concentrations compared to thecontrol. The results showed that FREPP has antioxidant and anti-inflammatory activities.

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Enechi, O. C., Okeke, E. S., Nwankwo, N. E., Nweze, J. E., Obilor, C. P., Okoye, C. I., & Awoh, O. E. (2020). Membrane Stabilization, Albumin Denaturation, Protease Inhibition, and Antioxidant Activity as Possible Mechanisms for the Anti-Inflammatory Effects of Flavonoid-Rich Extract of Peltophorum pterocarpum (DC.) K.Heyne (FREPP) Stem Bark: doi.org/10.26538/tjnpr/v4i10.25. Tropical Journal of Natural Product Research (TJNPR), 4(10), 812-816. https://tjnpr.org/index.php/home/article/view/1073
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Vol. 4 No. 10 (2020): Tropical Journal of Natural Product Research
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Articles
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Author Biographies

Emmanuel S. Okeke, Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria

Natural Science Unit, School of General Studies, University of Nigeria, Nsukka, Enugu State, Nigeria

School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, PR

Nicodemus E. Nwankwo, Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria

Natural Science Unit, School of General Studies, University of Nigeria, Nsukka, Enugu State, Nigeria

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