Hesperidin and Myricetin Attenuated Non-Alcoholic Fatty Liver Disease (NAFLD) in HepG2 Cells doi.org/10.26538/tjnpr/v4i10.14
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Abstract
Flavonoids are natural compounds that might have beneficial effects on non-alcoholic fatty liver disease (NAFLD). This study determined the efficacy of flavonoids in an NAFLD cell model. HepG2 cells were treated for 24 h with either 1 mM oleic acid (OA) or palmitic acid (PA) and 10 M of one of eleven flavonoids (4 flavones, 2 flavanones, and 5 flavonols) or fenofibrate. Cell morphology was examined by oil-red-O staining. Metabolic gene expression (PPAR α/γ, SREBP 1a/1c, ACC, ACOX, and FAS) was analyzed by RT/qPCR. All flavonoids and fenofibrate reduced intracellular lipid content. Hesperidin, myricetin, and all tested flavanones and flavonols prevented fatty acid-induced increases in metabolic gene expression but fenofibrate and some flavones did not suppress OA-induced FAS expression. Hesperidin and myricetin suppressed metabolic gene expression by more than fenofibrate indicating that they may provide potential pharmacological treatment options for regression of NAFLD status.
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