Oral and Acute Dermal Toxicity with Passiflora edulis Sims Aqueous Extract in Sprague-Dawley Rats doi.org/10.26538/tjnpr/v4i10.6
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Abstract
Passiflora edulis (Passion fruit) seeds is rich in phenolic compounds (bioflavonoids) that can inhibit lipid peroxidation and have a strong antioxidant effect. The present study investigated the acute oral and acute dermal toxicities of the ethanol extract of Passiflora edulis Sims fruit seeds. Acute oral toxicity of P. edulis S. extract was investigated in rats according to the Organisation for Economic Co-operation and Development (OECD) Guideline. The median lethal dose (LD50) was determined to be >15,000 mg/kg body weight. There was a significant increase in body weight (p < 0.05), and no death was recorded during the 14 days of the study. No overt changes were observed in the treated animals compared to controls. Overall, the results showed that oral administration of ethanol extract of P. edulis Sims. did not produce significant toxic effects in rats, indicating that the extract is relatively safe.
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References
Yang RL, Shi YH, Hao G, Li W, Le GW. Increasing Oxidative Stress with Progressive Hyperlipidemia in Human: Relation between Malondialdehyde and Atherogenic Index. J Clin Biochem Nutr. 2008;43(3):154- 158.
de Queiroz Mdo S, Janebro DI, da Cunha MA, Medeiros JdS, Sabaa-Srur AUO, Diniz MdFFM, Santos SCd. Effect of the yellow passion fruit peel flour (Passiflora edulis f. flavicarpa deg.) in insulin sensitivity in type 2 diabetes mellitus patients. Nutr J. 2012; 11:89.
Kandandapani S, Balaraman AK, Ahamed HN. Extracts of passion fruit peel and seed of Passiflora edulis (Passifloraceae) attenuate oxidative stress in diabetic rats. Chin J Nat Med. 2015; 13(9):680-686.
Kitada M, Ogura Y, Maruki-Uchida H, Sai M, Suzuki T, Kanasaki K, Hara Y, Seto H, Kuroshima Y, Monno I, Koya D. The Effect of Piceatannol from Passion Fruit (Passiflora edulis) Seeds on Metabolic Health in Humans. Nutrients 2017; 9(10):1142-1154.
Sari DK, Marianne, Lestari S, Laksmi LI. Differences in the Levels of Malondialdehyde, Total Cholesterol and Triglycerides after the Administration of a Passion Fruit Seed Ethanol Extract to Wistar Rats. Pak J Nutr. 2020; 19(1):11.
Zeraik ML, Serteyn D, Deby-Dupont G, Wauters J-H, Tits M, Yariwake JH, Angenot L, Franck T. Evaluation of the antioxidant activity of passion fruit (Passiflora edulis and Passiflora alata) extracts on stimulated neutrophils and myeloperoxidase activity assays. Food Chem 2011;
(2):259-265.
Maruki-Uchida H, Morita M, Yonei Y, Sai M. Effect of Passion Fruit Seed Extract Rich in Piceatannol on the Skin of Women: A Randomized, Placebo-Controlled, DoubleBlind Trial. J Nutr Sci Vitaminol (Tokyo) 2018; 64(1):75- 80.
Santos AA, Penha HA, Bellec A, Munhoz CdF, Pedrosaharand A, Bèrges H, Vieira MLC. Begin at the beginning: A BAC-end view of the passion fruit (Passiflora) genome. BMC Genom. 2014; 15:816.
Septembre-Malaterre A, Stanislas G, Douraguia E, Gonthier MP. Evaluation of nutritional and antioxidant properties of the tropical fruits banana, litchi, mango, papaya, passion fruit and pineapple cultivated in Reunion French Island. Food Chem. 2016; 212:225-233.
Neumann NR and Thompson TM. Medical Toxicology Education and Global Health: It is Still a World of Limited Resources in Low- and Middle-Income Countries. J Med Toxicol. 2020; 11:281-281.
Liu C, Wu H, Wang L, Luo H, Lu V, Zhang Q, Tang L, Wang Z. Farfarae Flos: A review of botany, traditional uses, phytochemistry, pharmacology, and toxicology. J Ethnopharmacol 2020:113038.
OECD. Acute Demal Toxicity 402. Paris: Organisation for Economic Co-operation and Development; 1987.
Maruki-Uchida H, Kurita I, Sugiyama K, Sai M, Maeda K, Ito T. The protective effects of piceatannol from passion fruit (Passiflora edulis) seeds in UVB-irradiated keratinocytes. Biol Pharm Bull. 2013; 36(5):845-849.
Matsui Y, Sugiyama K, Kamei M, Takahashi T, Suzuki T, Katagata Y, Ito T. Extract of passion fruit (Passiflora edulis) seed containing high amounts of piceatannol inhibits melanogenesis and promotes collagen synthesis. J Agric Food Chem. 2010; 58(20):11112-1118.
Ahmad R, Tripathi AK, Tripathi P, Singh S, Singh R, Singh RK. Malondialdehyde and protein carbonyl as biomarkers for oxidative stress and disease progression in patients with chronic myeloid leukemia. In Vivo. 2008; 22(4):525-528.
Martinez R, Torres P, Meneses MA, Figueroa JG, Pérez- Álvarez JA, Viuda-Martos M. Chemical, technological and in vitro antioxidant properties of mango, guava, pineapple and passion fruit dietary fibre concentrate. Food Chem. 2012; 135(3):1520-1526.