Ameliorative Effect and In-Vivo Antioxidant Properties of Methanol Extract of Burkea africana Stem Bark On Acetaminophen-Induced Hepatotoxicity in Rats
doi.org/10.26538/tjnpr/v4i2.3
Keywords:
Antioxidant,, Hepatoprotection,, Acetaminophen,, Burkea africana,, NephroprotectionAbstract
Burkea africana (Wild Syringa) is a wildly used plant in traditional medicine in Africa. It has been used for the treatment of hepatitis and other disorders. This study was aimed at investigating the liver and kidney protective potentials of the plant in acetaminophen-induced hepatic and nephrotoxicity in rats. Extraction was by cold maceration using 80% methanol. Thirty-six (36) rats were assigned into six groups of six rats each. Group 1 received distilled water, group 2 received silymarin and acetaminophen, group 3 received only acetaminophen. Groups 4-6 received plant extract at 200, 400 and 600 mg/kg b.wt and acetaminophen. Blood samples were taken for estimation of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP), bilirubin, total protein, albumin, serum urea, creatinine, malondialdehyde (MDA), catalase and glutathione. Significant increases (P<0.05) in the serum liver enzymes activities, bilirubin (total and direct), urea and creatinine were observed, while total proteins and albumin were significantly reduced (P<0.05) in the acetaminophen-treated group when compared to normal control. The extract-treated groups revealed significant (P<0.05) reduction in the activities of serum liver enzymes, bilirubin (total and direct), urea and creatinine values, with a significant (P<0.05) improvement in serum total proteins and albumin concentrations. The hepatoprotective and nephroprotective effects observed are probably due to the antioxidant activity of the extract. In conclusion methanol extract of Burkea. africana stem bark may possess hepatoprotective and nephroprotective activities and could be useful in the treatment of hepatitis and nephritis.
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