The Effect of Aqueous Extract of Ocimum gratissimum On Monosodium Glutamate Induced Biochemical Changes in Albino Rats
doi.org/10.26538/tjnpr/v4i2.1
Keywords:
Ocimum gratissimum,, Monosodium glutamate, Hepatic function,, Renal function.Abstract
The plant Ocimum gratissimum is used in folklore for the treatment of some diseases such as cancer, blood disorders and hepatitis. This study investigated the impact of Ocimum gratissimum leaf extract on monosodium glutamate-induced biochemical changes in albino rats. Fresh leaves of Ocimum gratissimum were procured in Oyingbo market in Lagos. The leaves were air-dried and the aqueous extract was prepared and used for the study. Acute toxicity of the extract was investigated by Lorke’s method. Twenty-five albino rats were randomized into 5 groups of five animals each. Group 1 was used as control, group 2 received no treatment while groups 3, 4 and 5 served as the treatment groups. Groups 2-5 were orally administered with 200 mg/kg body weight of monosodium glutamate for 14 days. This was followed with administration of 100, 200 and 300 mg/kg of Ocimum gratissimum leaf extract for groups 3, 4and 5, respectively. The animals were sacrificed after 14 days of treatment. Blood samples were obtained in all the groups and analyzed for renal and hepatic functions indices. The median lethal dose (LD50) of the extract was found to be 3800 mg/kg body weight. There was increase in weight of the rats after treatment, hepatic functions indices of alanine transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), total protein, albumin and rena function indices of urea and creatinine serum levels normalized after treatment. Occimum gratissimum aqueous extract affected some biochemical parameters induced by monosodium glutamate.
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