Exploring the Wonders of Kaempferia parviflora: A Comprehensive Review of its Botanical Characteristics, Extraction Methods, Pharmacological Characteristics, and Future Directions

Authors

  • Hafifah Hardini Master’s Program in Pharmaceutical Science, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55821, Indonesia
  • Yosi B. Murti Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55821, Indonesia
  •  Retno Murwant Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55821, Indonesia

DOI:

https://doi.org/10.26538/tjnpr/v9i8.4

Keywords:

Kaempferia parviflora, Black ginger, Phytochemicals, Extraction, Ethnopharmacology, Ethnobotany

Abstract

Kaempferia parviflora (KP), commonly known as black ginger, is an aromatic plant native to Thailand, notable for its richness in methoxyflavones—distinct compounds possessing potent pharmacological properties. Despite its promising bioactivity, the development of KP as a therapeutic agent remains insufficiently explored, with existing research yet to be fully integrated. This narrative review seeks to bridge traditional knowledge with contemporary pharmaceutical research by comprehensively examining the ethnomedicinal applications, extraction techniques, phytochemical composition, pharmacological investigations, conservation strategies, and by identifying research gaps and developmental challenges associated with KP. Relevant studies were selected from original publications in English or Indonesian, dated between 1980 and 2025, and sourced through the Scopus, PubMed, and Google Scholar databases. Findings indicate that KP is widely distributed across Southeast Asia. The primary methoxyflavones identified include 5,7,4′-trimethoxyflavone, 5,7-dimethoxyflavone, 3,5,7,3′,4′-pentamethoxyflavone; along with various essential oil constituents. Among conventional extraction techniques, maceration is considered most suitable, whereas microwave-assisted extraction (MAE) proves most efficient for isolating methoxyflavones, and supercritical CO₂ extraction is particularly effective for extracting essential oils. Traditionally used as an aphrodisiac, KP has garnered modern scientific interest for its anticancer, anti-obesity, and anti-inflammatory properties. Conservation efforts include in vitro culture, vivipary, and shade management. This review highlights the potential of KP as a promising source of novel therapeutic agents and emphasizes the need for further multidisciplinary research to explore its bioactive compounds and promote their sustainable utilization.

References

Yenjai C, Prasanphen K, Daodee S, Wongpanich V, Kittakoop P. Bioactive flavonoids from Kaempferia parviflora. Fitoterapia. 2004 Jan; 75(1):89–92.

Varghese BA, Nair RVR, Jude S, Varma K, Amalraj A, Kuttappan S. Green synthesis of gold nanoparticles using Kaempferia parviflora rhizome extract and their characterization and application as an antimicrobial, antioxidant and catalytic degradation agent. J Taiwan Inst Chem Eng. 2021 Sep; 126:166–172.

Nguyen DMC, Luong TH, Nghiem TC, Jung WJ. Chemical composition, antioxidant and antifungal activities of rhizome essential oil of Kaempferia parviflora Wall. ex Baker grown in Vietnam. J Appl Biol Chem. 2023 Jan; 66:15-22.

Lert-Amornpat T, Maketon C, Fungfuang W. Effect of Kaempferia parviflora on sexual performance in streptozotocin-induced diabetic male rats. Andrologia. 2017; 49(10).

Horigome S, Maeda M, Ho HJ, Shirakawa H, Komai M. Effect of Kaempferia parviflora extract and its polymethoxyflavonoid components on testosterone production in mouse testis-derived tumour cells. J Funct Foods. 2016 Oct; 26:529–538.

Takuathung MN, Potikanond S, Sookkhee S, Mungkornasawakul P, Jearanaikulvanich T, Chinda K, Wikan N, Nimlamool W. Anti-psoriatic and anti-inflammatory effects of Kaempferia parviflora in keratinocytes and macrophage cells. Biomed Pharmacother. 2021;143. Doi: 10.1016/j.biopha.2021.112229

Gustin C, Ringgit G, Siddiquee S, Saallah S, Kamaruzaman KAB. Optimal formulation of bitter gourd and black galingale extract: Evaluation of effects on inflammation and oxidative stress-related genes. Food Biosci. 2024 Dec; 62:104933.

Ishii M, Miyata H, Ikeda N, Sakurai T, Oura Y, Nishimura M. Kaempferia parviflora extract and its component polymethoxyflavones suppress adipogenic differentiation of human bone marrow-derived mesenchymal stem cells via the AMPK pathway. Mol Biol Rep. 2024 Jun; 51(1):785.

Yoshida I, Mishima T, Kumagai M, Takahashi Y, Fujita K, Igarashi T. Black ginger (Kaempferia parviflora) extract enhances circadian rhythm and promotes lipolysis in mice fed a high-fat diet. J Funct Foods. 2023 Aug; 107:105649.

Park GS, Shin J, Hong S, Saini RK, Gopal J, Oh JW. Evaluating the Diverse Anticancer Effects of Laos Kaempferia parviflora (Black Ginger) on Human Melanoma Cell Lines. Med Lith. 2024; 60(8).

Xia J, Xu M, Hu H, Zhang Q, Yu D, Cai M, Geng X, Zhang H, Zhang Y, Guo M, Lu D, Xu H, Li L, Zhang W. 5,7,4′-Trimethoxyflavone triggers cancer cell PD-L1 ubiquitin–proteasome degradation and facilitates antitumor immunity by targeting HRD1. MedComm. 2024; 5(7). Doi: 10.1002/mco2.611

Hairunisa I, Bakar MFA, Da’i M, Bakar FIA, Syamsul ES. Cytotoxic Activity, Anti-Migration and In Silico Study of Black Ginger (Kaempferia parviflora) Extract against Breast Cancer Cell. Cancers. 2023;15(10).

Sitthichai P, Chanpirom S, Maneerat T, Charoensup R, Tree-Udom T, Pintathong P, Laphookhieo S, Sripisut T. Kaempferia parviflora Rhizome Extract as Potential Anti-Acne Ingredient. Molecules. 2022; 27(14). Doi: 10.3390/molecules27144401

Leesombun A, Boonmasawai S, Nishikawa Y. Ethanol Extracts from Thai Plants have Anti-Plasmodium and Anti-Toxoplasma Activities In Vitro. Acta Parasitol. 2019 Jun; 64(2):257–261.

Mohamad AS, Nordin MN, Ani IC, Jemberang J, Ishak R, Hasan AN, Ahmad MI, Omar MR, Hamzah R. Preliminary studies of Volten VR4®

Kaempferia parviflora herb extracts on blood glucose levels in human type-2 diabetes mellitus and its mineral element analysis. Herba Pol.

2021;67(4):42–53.

Sripanidkulchai B, Somintara S, Pariwatthanakun C, Sripanidkulchai K, Leardkamolkarn V. Antidiabetic activity of methoxyflavone-enriched extract of Kaempferia parviflora in streptozotocin-induced diabetic rats. Songklanakarin J Sci Technol. 2020; 42(6):1239–1247.

Temviriyanukul P, Chansawhang A, Karinchai J, Phochantachinda S, Buranasinsup S, Inthachat W, Pitchakarn P, Chantong B. Kaempferia parviflora Extracts Protect Neural Stem Cells from Amyloid Peptide-Mediated Inflammation in Co-Culture Model with Microglia. Nutrients. 2023; 15(5). Doi: 10.3390/nu15051098

Prasanth MI, Malar DS, Brimson JM, Verma K, Tonsomboon A, Plaingam W, Tencomnao T. DAF-16 and SKN-1 mediate Anti-aging and Neuroprotective efficacies of “thai ginseng” Kaempferia parviflora Rhizome extract in Caenorhabditis elegans. Nutr Healthy Aging. 2022 Oct ;7(1–2):23–38. Doi: 10.3233/nha-210148

Chua LS, Abdullah FI, Awang MA. Potential of natural bioactive C-glycosyl flavones for antidiabetic properties. Studies in Natural Products Chemistry. Elsevier; 2020. p. 241–61. Available from: https://doi.org/10.1016/B978-0-12-817903-1.00008-5

Klinngam W, Rungkamoltip P, Thongin S, Joothamongkhon J, Khumkhrong P, Khongkow M, Namdee K, Tepaamorndech S, Chaikul P, Kanlayavattanakul M, Lourith N, Piboonprai K, Ruktanonchai U, Asawapirom U, Iempridee T. Polymethoxyflavones from Kaempferia parviflora ameliorate skin aging in primary human dermal fibroblasts and ex vivo human skin. Biomed Pharmacother. 2022;145. Doi:

10.1016/j.biopha.2021.112461

Lee S, Jang T, Kim KH, Kang KS. Improvement of Damage in Human Dermal Fibroblasts by 3,5,7-Trimethoxyflavone from Black Ginger (Kaempferia parviflora). Antioxidants. 2022 Feb ;11(2):425.

Singh KK, Mangang HA, Singh OK, Keithellakpam OS, Bhardwaj PK, Mukherjee PK, Ningombam DS, Akoijam S. Comprehensive Metabolomic and Bioactivity Profiling of Zingiberaceae Species From Manipur: Elucidating Antidiabetic and Antioxidant Mechanisms Through In Vitro and In Silico Approaches. Phytochem Anal. 2025

Cronin P, Ryan F, Coughlan M. Undertaking a literature review: a step-by-step approach. Br J Nurs. 2008 Jan; 17(1):38–43.

Paré G, Trudel MC, Jaana M, Kitsiou S. Synthesizing information systems knowledge: A typology of literature reviews. Inf Manag. 2015; 52(2):183–199.

Kress WJ, Prince LM, Williams KJ. The phylogeny and a new classification of the gingers (Zingiberaceae): evidence from molecular data. Am J Bot. 2002 Oct; 89(10):1682–1696.

Debnath S, Vijayan D. Diversity, phytogeographical distribution, endemism and conservation status of Zingiberaceae in India. Plant Sci Today. 2024;11(2):72–78.

Kam Y. Taxonomic studies in the genus Kaempferia (Zingiberaceae). Notes R Bot Gard Edinb. 1980.

Singh TA, Sarangi PK, Singh CB. High-Value Plants: Novel Insights and Biotechnological Advances. CRC Press; 2024. p. 271.

Catherine DL, Thohirah LA, Johnson S, NurAshikin PA, Maheran AA. Morphological Description for Kunyit Hitam (Kaempferia parviflora) and Breaking Bud Dormancy with BAP and Ethephon Treatments. Trans Malay Soc Plant Physiol. 2014.

Nattapon N, Jatuporn C, Thaya J, Jonsson K, Puangpaka S. Chromosome number variation and polyploidy in 19 Kaempferia (Zingiberaceae) taxa from Thailand and one species from Laos: Cytogenetics of Kaempferia. J Syst Evol. 2017;55(5):466–476.

Chen D, Li H, Li W, Feng S, Deng D. Kaempferia parviflora and Its Methoxyflavones: Chemistry and Biological Activities. Evid Based Complement Alternat Med. 2018; 4057456.

Thawtar MS, Kusano M, Yingtao L, Wunna, Thein MS, Tanaka K, Rivera M, Shi M,Watanabe KN. Exploring Volatile Organic Compounds in Rhizomes and Leaves of Kaempferia parviflora Wall. Ex Baker Using HS-SPME and GC–TOF/MS Combined with Multivariate Analysis. Metabolites. 2023 May;13(5):651.

Pham NK, Nguyen HT, Nguyen QB. A review on the ethnomedicinal uses, phytochemistry and pharmacology of plant species belonging to Kaempferia L. genus (Zingiberaceae). Pharm Sci Asia. 2020;48(1):1–24.

Yee TT, Lwin KWY. Study of Phytochemical Composition on Kaempferia parviflora Wall. ex Baker. IEEE-SEM. 2019 Jul;7(7):128–136.

Karim MA, Ardie SW, Khumaida N. Pematahan Dormansi Rimpang Kaempferia parviflora Wall. Ex Baker. Bul Agrohorti. 2014 Feb; 2(1):104.

Yusuf M, Chowdhury JU, Begum J. Ethno-medico-botanical knowledge from Kaukhali proper and Betbunia of Rangamati District. Bangladesh J Plant Taxon. 2006. Doi: 10.3329/bjpt.v13i1.594

Wuttidharmmavej W. Rattanakosin pharmaceutical scripture. Bangkok, Thailand: Wuttidharmmavej; 2002.

Wongsrikaew N, Kim H, Vichitphan K, Cho SK, Han J. Antiproliferative activity and polymethoxyflavone composition analysis of Kaempferia parviflora extracts. J Korean Soc Appl Biol Chem. 2012 Dec;55(6):813–817.

Sutthanut K, Sripanidkulchai B, Yenjai C, Jay M. Simultaneous identification and quantitation of 11 flavonoid constituents in Kaempferia parviflora by gas chromatography. J Chromatogr A. 2007 Mar; 1143(1):227–233.

Chaisuwan V, Dajanta K, Srikaeo K. Effects of extraction methods on antioxidants and methoxyflavones of Kaempferia parviflora. Food Res. 2022 Jun; 6(3):374–381.

Ninomiya K, Matsumoto T, Chaipech S, Miyake S, Katsuyama Y, Tsuboyama A, Fukutake M, Inoue M. Simultaneous quantitative analysis of 12 methoxyflavones with melanogenesis inhibitory activity from the rhizomes of Kaempferia parviflora. J Nat Med. 2016;70(2):179–189.

Tuntiyasawasdikul S, Limpongsa E, Jaipakdee N, Sripanidkulchai B. Transdermal permeation of Kaempferia parviflora methoxyflavones from isopropyl myristate-based vehicles. AAPS PharmSciTech. 2014;15(4):947–955.

Pitakpawasutthi Y, Palanuvej C, Ruangrungsi N. Quality evaluation of Kaempferia parviflora rhizome with reference to 5,7-dimethoxyflavone. J Adv Pharm Technol Res. 2018;9(1):26–30.

Pripdeevech P, Pitija K, Rujjanawate C, Pojanagaroon S, Kittakoop P, Wongpornchai S. Adaptogenic-active components from Kaempferia parviflora rhizomes. Food Chem. 2012;132(3):1150–1155.

Chaisuwan V, Dajanta K, Srikaeo K. Effects of extraction methods on antioxidants and methoxyflavones of Kaempferia parviflora. Food Res. 2022;6(3):374–381.

Handique SS, Kalita SPS, Lal G, Rawat DD. Extraction technologies for medicinal and aromatic plants. 1st ed. No. 66. Trieste, Italy: United Nations Industrial Development Organization and the International Centre for Science and High Technology; 2008. 260 p.

Rasul MG. Conventional extraction methods use in medicinal plants, their advantages and disadvantages. Int J Basic Clin Pharmacol. 2018;2(6):1–6.

Malakul W, Ingkaninan K, Sawasdee P, Woodman OL. The ethanolic extract of Kaempferia parviflora reduces ischaemic injury in rat isolated hearts. J Ethnopharmacol. 2011;137(1):184–191.

Sookkhee S, Sakonwasun C, Mungkornasawakul P, Khamnoi P, Wikan N, Nimlamool W. Synergistic effects of some methoxyflavones extracted from rhizome of Kaempferia parviflora combined with gentamicin against carbapenem-resistant strains of Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii. Plants. 2022;11(22):3067.

Klinngam W, Rungkamoltip P, Wongwanakul R, Joothamongkhon J, Du-a-man S, Khongkow M, Piboonprai K, Charoenphandhu N, Chatuphonprasert W. Skin rejuvenation efficacy and safety evaluation of Kaempferia parviflora standardized extract (BG100) in human 3D skin models and clinical trial. Biomolecules. 2024;14(7):776.

Huo C, Lee S, Yoo MJ, Lee BS, Jang YS, Kim HK, Park SY, Lee DS. Methoxyflavones from Black Ginger (Kaempferia parviflora Wall. ex Baker) and their Inhibitory Effect on Melanogenesis in B16F10 Mouse Melanoma Cells. Plants. 2023;12(5):1047.

Begum T, Gogoi R, Sarma N, Pandey SK, Lal M. Direct sunlight and partial shading alter the quality, quantity, biochemical activities of Kaempferia parviflora Wall., ex Baker rhizome essential oil: A high industrially important species. Ind Crops Prod. 2022;180:114765.

Luque de Castro MD, Priego-Capote F. Soxhlet extraction: Past and present panacea. J Chromatogr A. 2010;1217(16):2383–2399.

Liu WN, Shi J, Fu Y, Zhao XH. The stability and activity changes of apigenin and luteolin in human cervical cancer hela cells in response to heat treatment and Fe²⁺/Cu²⁺ addition. Foods. 2019;8(8):284.

De Santis D, Turchetti G. Hydrolates: Characteristics, Properties, and Potential Uses in the Food Industry. In: New Findings from Natural: Substances. 2022. p. 183–210.

Nisoa M, Plodkaew A, Sirisathitkul C, Wattanasit K, Somjit B, Pacdeepin P, Pinitsoontorn S. Simulation and experimentation on parameters influencing microwave-assisted extraction of bioactive compounds from Kaempferia parviflora rhizomes. Alex Eng J. 2023;65:357–366.

Krongrawa W, Limmatvapirat S, Saibua S, Limmatvapirat C. Optimization of Ultrasound-Assisted Extraction of Yields and Total Methoxyflavone Contents from Kaempferia parviflora Rhizomes. Molecules. 2022;27(13):4162.

Wongsrikaew N, Woo HC, Vichitphan K, Han J. Supercritical CO₂ for Efficient Extraction of Polymethoxyflavones in Kaempferia parviflora. ResGate. 2011;54(6):1008–1011.

Chaisuwan V, Dajanta K, Srikaeo K. Effects of extraction methods on antioxidants and methoxyflavones of Kaempferia parviflora. Food Res. 2022;6(3):374–381.

Ashokkumar M. Applications of ultrasound in food and bioprocessing. Ultrason Sonochem. 2015;25:17–23.

Devgun M, Nanda A, Ansari SH. Comparison of conventional and non conventional methods of extraction of heartwood of Pterocarpus marsupium Roxb. Acta Pol Pharm Drug Res. 2012;69(3):475–485.

Farooq S, Farooq S, Rather SA, Ganaie TA. Supercritical CO₂ extraction of natural products. In: Extraction of Natural Products from Agro-industrial Wastes: A Green and Sustainable Approach. 2023. p. 79–90.

Başer KHC, Buchbauer G. Handbook of Essential Oils: Science, Technology, and Applications. Boca Raton, FL: CRC Press/Taylor & Francis; 2010. 975 p.

D’Urso G, Zuzarte M, Formisano C, Bruno M, Rijo P, Senatore F. Headspace Solid-Phase Micro-Extraction Versus Hydrodistillation of Volatile Compounds from Leaves of Cultivated Mentha Taxa: Markers of Safe Chemotypes. Molecules. 2022;27(19):6561.

Jin S, Lee MY. Kaempferia parviflora extract as a potential anti-acne agent with anti-inflammatory, sebostatic and anti-Propionibacterium acnes activity. Int J Mol Sci. 2018;19(11):3218.

Krongrawa W, Limmatvapirat S, Vollrath MK, Kittakoop P, Saibua S, Limmatvapirat C. Fabrication, Optimization, and Characterization of Antibacterial Electrospun Shellac Fibers Loaded with Kaempferia parviflora Extract. Pharmaceutics. 2023;15(1):61.

Mala S, Thaweboon S, Luksamijarulkul P, Thaweboon B, Saranpuetti C, Kaypetch R. Effect of Kaempferia parviflora on Streptococcus mutans biofilm formation and its cytotoxicity. Key Eng Mater. 2018;773:328–332.

Kummee S, Tewtrakul S, Subhadhirasakul S. Antimicrobial activity of the ethanol extract and compounds from the rhizomes of Kaempferia parviflora. Songklanakarin J Sci Technol. 2008;30(4):463–466.

Klinjan P, Mungkornasawakul P, Tragoolpua Y, Sangthong P. Cytotoxicity and anti-herpes simplex virus type-1 activity of Kaempferia parviflora extract. Asia-Pac J Sci Technol. 2021;26(3).

Park SH, Park J, Lee M, Kim J, Eun S, Jun W, Lee JH. Antiobesity effect of Kaempferia parviflora accompanied by inhibition of lipogenesis and stimulation of lipolysis. Food Nutr Res. 2023;67:9370.

Lee HS, Jeon YE, Awa R, Yoshino S, Kim EJ. Kaempferia parviflora rhizome extract exerts anti-obesity effect in high-fat diet-induced obese C57BL/6N mice. Food Nutr Res. 2023;67:8949–8961.

Kobayashi H, Horiguchi-Babamoto E, Suzuki M, Makihara H, Tomozawa H, Tsubata M, Matsumoto T, Nakamura K, Yamamoto S. Effects of ethyl acetate extract of Kaempferia parviflora on brown adipose tissue. J Nat Med. 2016;70(1):54–61.

Yoshino S, Awa R, Miyake Y, Fukuhara I, Sato H, Ashino T, Yoshida A, Saito H. Daily intake of Kaempferia parviflora extract decreases abdominal fat in overweight and preobese subjects: A randomized, double-blind, placebo-controlled clinical study. Diabetes Metab Syndr Obes. 2018;11:447–458.

Kongdang P, Jaitham R, Thonghoi S, Kuensaen C, Pradit W, Ongchai S. Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways. Phytomedicine. 2019;59:152899.

Ongchai S, Chiranthanut N, Tangyuenyong S, Viriyakhasem N, Kongdang P. Kaempferia parviflora extract alleviated rat arthritis, exerted chondroprotective properties in vitro, and reduced expression of genes associated with inflammatory arthritis. Molecules. 2021;26(6):1703.

Kobayashi H, Suzuki R, Sato K, Ogami T, Tomozawa H, Tsubata M, Makihara H, Horiguchi-Babamoto E. Effect of Kaempferia parviflora extract on knee osteoarthritis. J Nat Med. 2018;72(1):136–144.

Julianti TB, Bakar MFA, Wikantyasning ER. Phytochemical, antioxidant analysis and in vitro xanthine oxidase inhibitory activity of Kaempferia parviflora and Kaempferia galanga. Trop J Nat Prod Res. 2022;6(12):1981–1985.

Kobayashi S, Kato T, Azuma T, Kikuzaki H, Abe K. Anti-allergenic activity of polymethoxyflavones from Kaempferia parviflora. J Funct Foods. 2015;13:100–107.

Tewtrakul S, Subhadhirasakul S, Kummee S. Anti-allergic activity of compounds from Kaempferia parviflora. J Ethnopharmacol. 2008;116(1):191–193.

Paramee S, Sookkhee S, Sakonwasun C, Na Takuathung M, Mungkornasawakul P, Nimlamool W, Wikan N, Smith DR, Potikanond S. Anti-cancer effects of Kaempferia parviflora on ovarian cancer SKOV3 cells. BMC Complement Altern Med. 2018;18(1):178.

Kim MH, Park S, Song G, Lim W, Han YS. Antigrowth effects of Kaempferia parviflora extract enriched in anthocyanidins on human ovarian cancer cells through Ca2+-ROS overload and mitochondrial dysfunction. Mol Cell Toxicol. 2022;18(3):383–391.

Thaklaewphan P, Ruttanapattanakul J, Monkaew S, Buatoom M, Sookkhee S, Nimlamool W, Wikan N, Smith DR, Potikanond S. Kaempferia parviflora extract inhibits TNF-α-induced release of MCP-1 in ovarian cancer cells through the suppression of NF-κB signaling. Biomed Pharmacother. 2021;141:111899.

Potikanond S, Sookkhee S, Takuathung MN, Mungkornasawakul P, Wikan N, Smith DR, Nimlamool W. Kaempferia parviflora extract exhibits anti-cancer activity against HeLa cervical cancer cells. Front Pharmacol. 2017;8:667.

Suradej B, Sookkhee S, Panyakaew J, Mungkornasawakul P, Wikan N, Smith DR, Nimlamool W, Potikanond S. Kaempferia parviflora extract inhibits STAT3 activation and interleukin-6 production in HeLa cervical cancer cells. Int J Mol Sci. 2019;20(17):4377.

Azuma T, Kayano S, Matsumura Y, Konishi Y, Tanaka Y, Kikuzaki H. Antimutagenic and α-glucosidase inhibitory effects of constituents from Kaempferia parviflora. Food Chem. 2011;125(2):471–475.

Nemidkanam V, Kato Y, Kubota T, Chaichanawongsaroj N. Ethyl acetate extract of Kaempferia parviflora inhibits Helicobacter pylori-associated mammalian cell inflammation by regulating proinflammatory cytokine expression and leukocyte chemotaxis. BMC Complement Med Ther. 2020;20(1):124.

Wattanapitayakul SK, Suwatronnakorn M, Chularojmontri L, Herunsalee A, Niumsakul S, Charuchongkolwongse S, Pompimon W, Satayavivad J. Kaempferia parviflora ethanolic extract promoted nitric oxide production in human umbilical vein endothelial cells. J Ethnopharmacol.

2007;110(3):559–562.

Rujjanawate C, Kanjanapothi D, Amornlerdpison D, Pojanagaroon S. Anti-gastric ulcer effect of Kaempferia parviflora. J Ethnopharmacol. 2005;102(1):120–122.

Tonsomboon A, Prasanth MI, Plaingam W, Tencomnao T. Kaempferia parviflora rhizome extract inhibits glutamate-induced toxicity in HT-22 mouse hippocampal neuronal cells and extends longevity in Caenorhabditis elegans. Biology. 2021;10(4):311.

Wattanathorn J, Tong-Un T, Muchimapura S, Wannanon P, Sripanidkulchai B, Phachonpai W. Anti-stress effects of Kaempferia parviflora in immobilization subjected rats. Am J Pharmacol Toxicol. 2013;8(1):31–38.

Welbat JU, Chaisawang P, Chaijaroonkhanarak W, Prachaney P, Pannangrong W, Sripanidkulchai B, Chaijaroonkhanarak W. Kaempferia parviflora extract ameliorates the cognitive impairments and the reduction in cell proliferation induced by valproic acid treatment in rats. Ann Anat. 2016;206:7–13.

Plaingam W, Sangsuthum S, Angkhasirisap W, Tencomnao T. Kaempferia parviflora rhizome extract and Myristica fragrans volatile oil increase the levels of monoamine neurotransmitters and impact the proteomic profiles in the rat hippocampus: Mechanistic insights into their

neuroprotective effects. J Tradit Complement Med. 2017;7(4):538–552.

Hawiset T, Muchimapura S, Wattanathorn J, Sripanidkulchai B. Screening neuropharmacological activities of Kaempferia parviflora (krachai dam) in healthy adult male rats. Am J Appl Sci. 2011;8(7):695–702.

Youn K, Lee J, Ho CT, Jun M. Discovery of polymethoxyflavones from black ginger (Kaempferia parviflora) as potential β-secretase (BACE1) inhibitors. J Funct Foods. 2016;20:567–574.

Mekjaruskul C, Yang YT, Leed MGD, Sadgrove MP, Jay M, Sripanidkulchai B. Novel formulation strategies for enhancing oral delivery of methoxyflavones in Kaempferia parviflora by SMEDDS or complexation with 2-hydroxypropyl-β-cyclodextrin. Int J Pharm. 2013;445(1–2):1–11.

Chaturapanich G, Chaiyakul S, Verawatnapakul V, Pholpramool C. Effects of Kaempferia parviflora extracts on reproductive parameters and spermatic blood flow in male rats. Reproduction. 2008;136(4):515-522. Doi:10.1530/REP-08-0069

Phung HM, Lee S, Hong S, Lee S, Jung K, Kang KS. Protective effect of polymethoxyflavones isolated from Kaempferia parviflora against TNF-α-induced human dermal fibroblast damage. Antioxidants. 2021;10(10):1609.

Choi MH, Kim KH, Yook HS. Antioxidant activity and development of cosmetic materials of solvent extracts from Kaempferia parviflora. J Korean Soc Food Sci Nutr. 2018;47(4):414–421.

Thao NP, Luyen BTT, Lee SH, Jang HD, Kim YH. Anti-osteoporotic and antioxidant activities by rhizomes of Kaempferia parviflora Wall. ex Baker. Nat Prod Sci. 2016;22(1):13–20.

Julianti TB, Bakar MFA, Wikantyaningsih ER. Phytochemical, antioxidant analysis and in vitro xanthine oxidase inhibitory activity of Kaempferia parviflora and Kaempferia galanga. 2022;6(12):1981-1985.

Takuathung MN, Potikanond S, Sookkhee S, Mungkornasawakul P, Jearanaikulvanich T, Chinda K, Tantisira MH. Anti-psoriatic and anti-inflammatory effects of Kaempferia parviflora in keratinocytes and macrophage cells. Biomed Pharmacother. 2021;143:112229.

Horigome S, Yoshida I, Tsuda A, Harada T, Yamaguchi A, Yamazaki K, Takahashi M. Identification and evaluation of anti-inflammatory compounds from Kaempferia parviflora. Biosci Biotechnol Biochem. 2014;78(5):851–860.

Promthep K, Eungpinichpong W, Sripanidkulchai B, Chatchawan U. Effect of Kaempferia parviflora extract on physical fitness of soccer players: A randomized double-blind placebo-controlled trial. Med Sci Monit Basic Res. 2015;21:100–108.

Sripanidkulchai B, Promthep K, Tuntiyasawasdikul S, Tabboon P, Areemit R. Supplementation of Kaempferia parviflora extract enhances physical fitness and modulates parameters of heart rate variability in adolescent student-athletes: A randomized, double-blind, placebo-controlled clinical study. J Diet Suppl. 2022;19(2):149–167.

Wattanathorn J, Muchimapura S, Tong-Un T, Saenghong N, Thukhum-Mee W, Sripanidkulchai B. Positive modulation effect of 8-week consumption of Kaempferia parviflora on health-related physical fitness and oxidative status in healthy elderly volunteers. Evid-Based Complement Altern Med ECAM. 2012;2012:732816.

Toda K, Hitoe S, Takeda S, Shimoda H. Black ginger extract increases physical fitness performance and muscular endurance by improving inflammation and energy metabolism. Heliyon. 2016;2(5).

Tep-Areenan P, Ingkaninan K, Randall MD. Mechanisms of Kaempferia parviflora extract (KPE)-induced vasorelaxation in the rat aorta. Asian Biomed. 2010;4(1):103–111.

Tep-Areenan P, Sawasdee P, Randall M. Possible mechanisms of vasorelaxation for 5,7-dimethoxyflavone from Kaempferia parviflora in the rat aorta. Phytother Res PTR. 2010;24(10):1520–1525.

Weerateerangkul P, Palee S, Chinda K, Chattipakorn SC, Chattipakorn N. Effects of Kaempferia parviflora Wall. ex Baker and sildenafil citrate on cGMP level, cardiac function, and intracellular Ca2+ regulation in rat hearts. J Cardiovasc Pharmacol. 2012;60(3):299–309.

Horigome S, Yoshida I, Ito S, Inohana S, Fushimi K, Nagai T, Tanaka M. Inhibitory effects of Kaempferia parviflora extract on monocyte adhesion and cellular reactive oxygen species production in human umbilical vein endothelial cells. Eur J Nutr. 2017;56(3):949–964.

Kafindra L, Khumaida N, Wahyuning Ardie S. Induksi rimpang mikro Kaempferia parviflora secara in vitro dengan penambahan BAP dan sukrosa. J Hortik Indones. 2015;6(1):54–63.

Rajashree P, Reena P. Influence of thidiazuron on in vitro regeneration potential of Kaempferia parviflora Wall. ex Baker from Eastern India. Res J Biotechnol. 2023;18(9):18–22.

Jamilkhair NA, Osman NI, Ismail NH, Jantan MA, Awal A. Establishment of explant sterilization protocol and the effects of BAP and AgNO₃ on in vitro multiplication of Kaempferia parviflora Wall. ex Baker. Malays J Chem. 2024;26(1):332–341.

Koh KS, Ismail MF, Naharudin NS, Gantait S, Sinniah UR. Harnessing the potential of transverse thin cell layer culture for high-frequency micropropagation of Thai ginseng (Kaempferia parviflora Wall. ex Baker). Ind Crops Prod. 2024;213:118375.

Devi AR, Mini Raj N, Minimol JS. Vivipary in Kaempferia parviflora Wall. ex Baker: Discovery and utilization. J Trop Agric. 2022;60(2):187–194.

Labrooy CD, Abdullah TL, Abdullah NAP, Stanslas J. Optimum shade enhances growth and 5,7-dimethoxyflavone accumulation in Kaempferia parviflora Wall. ex Baker cultivars. Sci Hortic. 2016;213:346–353.

Downloads

Published

2025-08-31

How to Cite

Exploring the Wonders of Kaempferia parviflora: A Comprehensive Review of its Botanical Characteristics, Extraction Methods, Pharmacological Characteristics, and Future Directions. (2025). Tropical Journal of Natural Product Research , 9(8), 3465-3479. https://doi.org/10.26538/tjnpr/v9i8.4