In Vitro anti-inflammatory and antiviral activities of Pluchea indica (L.) Less. extracts on human monocytes
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Abstract
Pluchea indica (L.) Less., a halophyte from the Asteraceae family, thrives in saline soils and has traditional medicinal uses. This study aimed to investigate the in vitro antioxidant activity, as well as the anti-inflammatory and antiviral potential of ethanol extracts from P. indica. The crude extract was obtained by refluxing P. indica with 70% ethanol. Phytochemical analysis was conducted, and antioxidant properties were assessed. Toxicity was evaluated in white blood cells, red blood cells, and the THP-1 cell line. The anti-inflammatory effects were analyzed by measuring TNF-α and TGF-β mRNA expression, while antiviral properties were assessed via IFN-β, TLR3, TLR7, TLR8, and TLR9 mRNA expression in THP-1 cells using quantitative RT-PCR. All ethanol extracts contained alkaloids, phenolics, flavonoids, coumarins, tannins, and terpenoids; however, steroids were only present in the flower extract. Higher levels of phenolic and flavonoid compounds were found in the leaf and flower extracts, correlating with antioxidant activity. None of the extracts exhibited cytotoxicity to immune cells. The leaf extract demonstrated anti-inflammatory properties by decreasing TNF-α mRNA expression while stimulating TGF-β mRNA expression. The root extract showed antiviral effects by inducing IFN-β mRNA expression. Additionally, the stem extract exhibited both anti-inflammatory and antiviral properties by reducing TNF-α mRNA expression and enhancing the expression of TGF-β, IFN-β, TLR3, TLR8, and TLR9 mRNA. This study indicates that ethanol extracts of P. indica, prepared via reflux extraction, possess in vitro antioxidant, anti-inflammatory, and antiviral properties in human immune cells, suggesting their potential as therapeutic agents for inflammatory and viral diseases.
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References
1. Ferreira MJ, Pinto DCGA, Cunha Â, Silva H. Halophytes as Medicinal Plants against Human Infectious Diseases. Appl Sci. 2022; 12(15):1-18.
2. Patcharapreecha P, Topark-Ngarm B, Goto I, Kimura M. Studies on saline soils in khon kaen region, northeast thailand I. Physical and chemical properties of saline soils. Soil Sci Plant Nutr. 1989; 35(2):171–179.
3. Liu L, Wang B. Protection of Halophytes and Their Uses for Cultivation of Saline-Alkali Soil in China. Biology (Basel). 2021; 10(353):1–11.
4. Deeprom S, Trelo-Ges V, Topark-Ngarm B, Homchuen S, Kummee T. Plant Diversity on Slightly Saline Soils in Chi River Basin of Northeast Thailand. Int J Environ Rural Dev. 2015; 6(1):40–45.
5. Cho C-L, Lee Y-Z, Tseng C-N, Cho J, Cheng Y-B, Wang K-W, Chen H-J, Chiou S-J, Chou C-H, Hong Y-R. Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells. Biomed Rep. 2017; 7(5):416–422
6. Sen T, Chaudhuri AKN. Antiinflammatory evaluation of a Pluchea indica root extract. J Ethnopharmacol. 1991; 33(1–2):135–141.
7. Trang VM, Son NT, Pham TV, Giang PM. Essential Oils from the Leaves and Stem Barks of Pluchea Indica (L.) Less.: Chemical Analysis, Cytotoxicity, Anti-inflammation, Antimicrobial Activity, Molecular Docking, and ADMET Profiling. Chem Biodivers. 2024; 21(12):e202401785.
8. Srisook K, Jinda S, Srisook E. Anti-inflammatory and antioxidant effects of Pluchea indica leaf extract in TNF-α-induced human endothelial cells. Walailak J Sci Technol. 2021; 18(10):1–12.
9. Rolnik A, Olas B. The plants of the asteraceae family as agents in the protection of human health. Int J Mol Sci. 2021; 22(6):1–10.
10. Sankla N and Sunthamala N. Phytochemical screening, Antioxidant Activity and Ex Vivo Immunological-Associated Properties of a Halophyte Extract, Maytenus mekongensis In Sched. Trop J Nat Prod Res Available. 2021; 5(11):1949–1957.
11. Sankla N, Loutchanwoot P, Khankhum S, Khammuang S, Sarnthima R, Sunthamala N. In vitro Antioxidant and Immunological-Associated Activities of Ethanol Extracts of Azima sarmentosa (Blume) Benth. & Hook. F. Trop J Nat Prod Res. 2022; 6(12):2007–2013.
12. Cao X, Zhang Y, Xun H, Wang J, Tang F. High-Yield Recovery of Antioxidant Compounds from Bambusa chungii Culms Using Pressurized Hot Water Extraction. Antioxidants. 2022; 11(11):1-20.
13. Ayoola GA, Coker HAB, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC, Atangbayila TO. Phytochemical Screening and Antioxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria. Trop J Pharm Res. 2008; 7(3).1019-1024.
14. Chan EWC, Ng YK, Wong SK, Chan HT. .Pluchea indica: An updated review of its botany, uses, bioactive compounds and pharmacological properties. Pharmaceu. Sci. Asia. 2022; 49(1):77–85.
15. Mutha RE, Tatiya AU, Surana SJ. Flavonoids as natural phenolic compounds and their role in therapeutics: an overview. Futur J Pharm Sci. 2021; 7(1).1-13.
16. Nopparat J, Nualla-Ong A, Phongdara A. Ethanolic extracts of Pluchea indica (L.) leaf pretreatment attenuates cytokine-induced β-cell apoptosis in multiple low-dose streptozotocin-induced diabetic mice. PLoS One. 2019; 14(2):1–19.
17. Noridayu AR, Hii YF, Faridah A, Khozirah S, Lajis N. Antioxidant and antiacetylcholinesterase activities of Pluchea indica Less. Int Food Res J. 2011; 18(3):925–929.
18. Magnavacca A, Sangiovanni E, Racagni G, 'Agli MD. The antiviral and immunomodulatory activities of propolis: An update and future perspectives for respiratory diseases. Med Res Rev. 2022; 42:897-945.
19. Buranasukhon W, Athikomkulchai S, Tadtong S, Chittasupho C. Wound healing activity of Pluchea indica leaf extract in oral mucosal cell line and oral spray formulation containing nanoparticles of the extract. Pharm Biol. 2017; 55(1):1767–1774.
20. Pramanik KC, Bhattacharya P, Biswas R, Bandyopadhyay D, Mishra M, Chatterjee T. Hypoglycemic and antihyperglycemic activity of leaf extract of Pluchea indica Less. Orient Pharm Exp Med. 2006; 6(3):232–236.
21. Sirichaiwetchakoon K, Churproong S, Kupittayanant S, Eumkeb G. The Effect of Pluchea indica (L.) Less. Tea on Blood Glucose and Lipid Profile in People with Prediabetes: A Randomized Clinical Trial. J Altern Complement Med. 2021; 27(8):669-677.
22. Jang DI, Lee AH, Shin HY, Song HR, Park JH, Kang TB, Lee SR, Yang SH. The Role of Tumor Necrosis Factor Alpha (TNF-α) in Autoimmune Disease and Current TNF-α Inhibitors in Therapeutics. Int J Mol Sci. 2021; 22(5):1-16.
23. Raschick M, Richter A, Fischer L, Knopf L, Schult A, Yakupov R, Behnisch G, Guttek K, Düzel E, Dunay IR, Seidenbecher CI, Schraven B, Reinhold G, Schott BH. Plasma concentrations of anti-inflammatory cytokine TGF-β are associated with hippocampal structure related to explicit memory performance in older adults. J Neural Transm. 2023; 130(8):989–1002.
24. Vanaki N, Aslani S, Jamshidi A, Mahmoudi M. Role of innate immune system in the pathogenesis of ankylosing spondylitis. Biomed Pharmacother. 2018; 105:130–143.
25. Abbas AK, Lichtman AH, Pillai S, Baker DL. Cellular and molecular immunology. (10th ed.). Pennsylvania: Philadelphia; 2022. 1-587 p.
26. Locher CP, Witvrouw M, De Bethune MP, Burch MT, Mower HF, Davis H, Lasure A, Pauwels R, Clercq ED, Vlietinck AJ. Antiviral activity of Hawaiian medicinal plants against human immunodeficiency virus type-1 (HIV-1). Phytomedicine. 1996; 2(3):259–264.
27. Hornung V, Rothenfusser S, Britsch S, Krug A, Jahrsdörfer B, Giese T, Endres S, Hartmann G. Quantitative Expression of Toll-Like Receptor 1–10 mRNA in Cellular Subsets of Human Peripheral Blood Mononuclear Cells and Sensitivity to CpG Oligodeoxynucleotides. J Immunol. 2002; 168(9):4531–4537.
28. Hemmi H, Akira S. TLR signalling and the function of dendritic cells. Chem Immunol Allergy. 2005; 86:120–135.
29. Chen Y, Lin J, Zhao Y, Ma X, Yi H. Toll-like receptor 3 (TLR3) regulation mechanisms and roles in antiviral innate immune responses. J Zhejiang Univ Sci B. 2021; 22(8):609–32.