The Effect of Apigenin from Peronema canescens on Ovarian Follicular Development in Female Albino Mice

Abstract

Reproductivity is an important aspect of overall well-being for individuals and populations. The World Health Organization (WHO) estimates that infertility cases in the world are around 8%–10%. Sungkai leaves (Peronema canescens Jack.) contain apigenin compounds which are flavonoid derivatives. Apigenin as an antioxidant helps neutralise free radicals by protecting the ovaries from damage. This study aims to determine the effect of administering active isolates of sungkai leaf on the fertility of female albino mice. Twenty-four female mice divided into four groups of six each were used in this study. The test group was given an oral suspension of active apigenin isolate of sungkai leaves at doses of 1 mg/kg bw, 25 mg/kg bw, and 50 mg/kg bw for 14 days. The negative control group was only given a 0.5% Sodium CMC suspension. On the 15th day, the mice were sacrificed under inhalation anaesthesia using ether, both ovaries were removed, and histopathological preparations were made to determine the number of follicles. The results showed an increase in the number of primary, secondary and de graaf ovarian follicles. The increase in the number of ovaries was optimal at a dose of 50 mg/kg. This study concluded that apigenin isolated from sungkai leaves has the potential to enhance fertility in women.