Synthesis and characterization of copper nanoparticles using Allium cepa (L.) outer peel at ambient temperature
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Abstract
Nanotechnology is increasingly finding applications in various fields like material engineering, medicine, and environmental science. Due to their antimicrobial, catalytic, and electrical properties, copper nanoparticles (CuNPs) have been used in biomedical treatments, cleaning up the environment, and making electronic devices. However, toxic chemicals and high energy consumption are the challenges of conventional synthesis methods of nanoparticles. This study explores the eco-friendly synthesis of copper nanoparticles (CuNPs) using the outer peel extract of Allium cepa L. at ambient temperature (25 ± 2°C). The phytochemicals in the extract were used as reducing agents in the process. The formation of CuNPs was confirmed by a color change to dark brown when they encountered Cu2+ ions. Using UV–Visible spectroscopy to characterize the sample showed broad absorbance band around 380-600 nm, which means nanoparticles are forming. Using Fourier Transform Infrared (FTIR) analysis, functional groups like hydroxyl (O-H), Cu-H, and C-C were found to be reducing Cu ions. X-Ray Diffractometry (XRD) confirmed the crystalline structure of the CuNPs, while Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) showed pseudo-spherical, rod-like, and polygonal particles with diameters ranging from 8.31 to 179.75 nm. Energy Dispersive X-Ray Spectroscopy (EDS) analysis indicated the presence of copper, oxygen, and sulfur. The results indicate a sustainable alternative for advanced nanotechnology synthesis and applications by demonstrating an efficient, cost-effective, and environmentally friendly approach to synthesizing CuNPs under ambient conditions.
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References
Phang YK, Aminuzzaman M, Akhtaruzzaman Md MG, Ogawa S, Watanabe A. Green synthesis and characterization of cuo nanoparticles derived from papaya peel extract for the photocatalytic degradation of palm oil mill effluent (Pome). Sustainability. 2021; 13(2):796. https://doi.org/10.3390/su13020796
Arun L, Karthikeyan C, Philip D, Unni C. Optical, magnetic, electrical, and chemo-catalytic properties of bio-synthesized CuO/NiO nanocomposites. J. Phys Chem Solids. 2020; 136:109155. https://doi.org/10.1016/j.jpcs.2019.109155.
Naseem T, Durrani T. The role of some important metal oxide nanoparticles for wastewater and antibacterial applications: A review. Environ Chem Ecotoxicol. 2021; 3:59-75. https://doi.org/10.1016/j.enceco.2020.12.001
El-Khawaga AM, Zidan A, Abd El-Mageed AI. Preparation methods of different nanomaterials for various potential applications: A review. J. Mol Struct. 2023; 1281:135148. https://doi.org/10.1016/j.molstruc.2023.135148
Amini SM. Preparation of antimicrobial metallic nanoparticles with bioactive compounds. Mater Sci Eng C. 2019; 103:109809. https://doi.org/10.1016/j.msec.2019.109809
Hatipoğlu A. Rapid green synthesis of gold nanoparticles: Synthesis, characterization, and antimicrobial activities. Prog. Nutr. 2021; 23:e2021242.
Atalar MN, Baran A, Baran MF, Keskin C, Aktepe N, Yavuz Ö, İrtegun Kandemir S. Economic fast synthesis of olive leaf extract and silver nanoparticles and biomedical applications. Part Sci. Technol. 2022; 40(5):589-597. https://doi.org/10.1080/02726351.2021.1977443
Baran MF, Keskin C, Baran A, Hatipoğlu A, Yildiztekin M, Küçükaydin S, Kurt K, Hoşgören H, Sarker MMR, Sufianov A, Beylerli O. Green synthesis of silver nanoparticles from Allium cepa L. Peel extract, their antioxidant, antipathogenic, and anticholinesterase activity. Molecules. 2023; 28(5):2310. https://doi.org/10.3390/molecules28052310
Nahar K, Aziz S, Bashar MS, Haque MA, Al-Reza S. Synthesis and characterization of Silver nanoparticles from Cinnamomum tamala leaf extract and its antibacterial potential. Int. J. Nano Dimens. 2020; 11:88–98.
Hano C, Abbasi BH. Plant-Based Green Synthesis of Nanoparticles: Production, Characterization and Applications. Biomolecules. 2021; 12(1):31. https://doi.org/10.3390/biom12010031
Majid M, Farhan A, Asad MI, Khan MR, Hassan SS, Haq IU, Bungau S. An extensive pharmacological evaluation of new anti-cancer triterpenoid (nummularic acid) from Ipomoea batatas through in vitro, in silico, and in vivo studies. Molecules. 2022; 27:2474.
https://doi.org/10.3390/molecules27082474
Qing Y, Cheng L, Li R, Liu G, Zhang Y, Tang X, Wang J, Liu H, Qin Y. Potential antibacterial mechanism of silver nanoparticles and the optimization of orthopedic implants by advanced modification technologies. Int J. Nanomed. 2018; 13(1):3311–3327. https://doi.org/10.2147/IJN.S165125
Losasso C, Belluco S, Cibin V, Zavagnin P, Mičetić I, Gallocchio F, Zanella M, Bregoli L, Biancotto G, Ricci A. Antibacterial activity of silver nanoparticles: Sensitivity of different Salmonella serovars. Front Microbiol. 2014; 5:227. https://doi.org/10.3389/fmicb.2014.00227
Aminuzzaman M, Kei LM, Liang WH. Green synthesis of copper oxide (CuO) nanoparticles using banana peel extract and their photocatalytic activities. AIP Conf Proc. 2017; 1828:1–5. https://doi.org/10.1063/1.4979387
Sharmila G, Pradeep RS, Sandiya K, Santhiya S, Muthukumaran C, Jeyanthi J, Kumar NM, Thirumarimurugan M. Biogenic synthesis of CuO nanoparticles using Bauhinia tomentosa leaves extract: Characterization and its antibacterial application. J. Mol Struct. 2018; 1165:288–292.
https://doi.org/10.1016/j.molstruc.2018.04.011
Chowdhury R, Khan A, Rashid MH. Green synthesis of CuO nanoparticles using Lantana camara flower extract and their potential catalytic activity towards the aza-Michael reaction. RSC Adv. 2020; 10:14374–14385.
Samari F, Baluchi L, Salehipoor H, Yousefinejad S. Conrollable phyto-synthesis of cupric oxide by aqueous extract of Capparis spinosa (caper) leaves and its application in iron sensing. Microchip J. 2019; 150:1-10. https://doi.org/10.1016/j.microc.2019.104158
Sharma S, Kumar K. Aloe-vera leaf extract as a green agent for the synthesis of CuO nanoparticles inactivating bacterial pathogens and dye. J. Disper Sci Technol. 2020; 41: 1–13. https://doi.org/10.1080/01932691.2020.1791719
Induja MP, Geetha RV. Antimicrobial activity of Allium cepa against bacteria causing enteric infection. Drug Invent Today. 2018; 10(12).
Loredana L, Giuseppina A, Filomena N, Florinda F, Marisa DM, Donatella A. Biochemical, antioxidant properties and antimicrobial activity of different onion varieties in the Mediterranean area. J. Food Meas Charact. 2019; 13: 1232-1241. https://doi.org/10.1007/s11694-019-00038-2
Sharma K, Mahato N, Lee YR. Systematic study on active compounds as antibacterial and antibiofilm agent in aging onions. J. Food Drug Anal. 2018; 26(2): 518–528. https://doi.org/10.1016/j.jfda.2017.06.009
Bystrická J, Musilová J, Vollmannová A, Timoracká M, Kavalcová P. Bioactive components of onion (Allium cepa L.)—A Review. Acta Aliment. 2013; 42:11–22. https://doi.org/10.1556/AAlim.42.2013.1.2
Zhao XX, Lin FJ, Li H, Li HB, Wu DT, Geng F, Ma W, Wang Y, Miao BH, Gan RY. Recent Advances in Bioactive Compounds, Health Functions, and Safety Concerns of Onion (Allium cepa L.). Front Nutr. 2021; 8:669805. https://doi.org/10.3389/fnut.2021.669805
Magry MA, Narula SA. Sustainability of agri-food supply chains through innovative waste management models. In: Valorization of agri-food wastes and by-products. Academic Press; 2021. p. 591-605.
Nnoli EC, Ibeneme UJ, Omokaro GO, Osarhiemen IO, Ewubare PO, Aliyu SO, et al. Food waste and loss management—causes, effects and possible solutions from a Nigeria context. Am J. Food Sci Technol. 2024; 3(1):30-41.
Kolawole ID, Kolawole GO, Sanni-Manuel BA, Kolawole SK, Ewansiha JU, Kolawole VA, Kolawole FO. Economic impact of waste from food, water, and agriculture in Nigeria: challenges, implications, and applications—a review. Discov Environ. 2024; 2(1):51.
Chaudhary S, Jain VP, Sharma D, Jaiswar G. Implementation of agriculture waste for the synthesis of metal oxide nanoparticles: its management, future opportunities and challenges. J Mater Cycles Waste Manag. 2023; 25(6):3144-3160.
Islam MF, Miah MAS, Huq AO, Saha AK, Mou ZJ, Mondol MMH, Bhuiyan MNI. Green synthesis of zinc oxide nanoparticles using Allium cepa L. waste peel extracts and its antioxidant and antibacterial activities. Heliyon. 2024; 10(3):e12345.
Shweta S, Kaur G. A sustainable synthesis, characterization of modified waste onion peels and its exploration in various applications. In: E3S Web Conf. EDP Sciences; 2024. p. 01077.
Villagrán Z, Anaya-Esparza LM, Velázquez-Carriles CA, Silva-Jara JM, Ruvalcaba-Gómez JM, Aurora-Vigo EF, Martínez-Esquivias F. Plant-based extracts as reducing, capping, and stabilizing agents for the green synthesis of inorganic nanoparticles. Resources. 2024; 13(6):70.
Daylee AK. Green synthesis of copper nanoparticles using Myrtus communis leaves extract: characterization and applications [dissertation]. University of Kerbala; 2022.
Amjad R, Mubeen B, Ali SS, Imam SS, Alshehri S, Ghoneim MM, Kazmi I. Green synthesis and characterization of copper nanoparticles using Fortunella margarita leaves. Polymers (Basel). 2021; 13(24):4364.
Sajid M, Płotka-Wasylka J. Nanoparticles: synthesis, characteristics, and applications in analytical and other sciences. Microchem J. 2020; 154:104623.
Mohammed MS, Kadhim SE. Study of the optical and structural properties of copper nanoparticles prepared via the electrochemical technique. J. Opt. 2024; 1-6.
Patel K, Patel A, Jethwa VP, Patel H, Solanki GK. X-ray diffraction analysis of orthorhombic SnSe nanoparticles by Williamson–Hall, Halder–Wagner and size–strain plot methods. Chem Phys Impact. 2024; 8:100547.
Devaraji M, Thanikachalam PV, Elumalai K. The potential of copper oxide nanoparticles in nanomedicine: a comprehensive review. Biotechnol Notes. 2024.
Anwaar S, Altaf F, Anwar T, Qureshi H, Siddiqi EH, Soufan W, Zaman W. Biogenic synthesis of copper oxide nanoparticles using Eucalyptus globulus leaf extract and its impact on germination and phytochemical composition of Lactuca sativa. Sci Rep. 2024; 14(1):1-15.
Amatya SP, Joshi LP. Bio-synthesis of copper nanoparticles (CuNPs) using garlic extract to investigate antibacterial activity. Bibechana. 2020; 17:13-19.
Ismail MIM. Green synthesis and characterizations of copper nanoparticles. Mater Chem Phys. 2020; 240:122283.
Ginting B, Maulana I, Karnila I. Biosynthesis of copper nanoparticles using Blumea balsamifera leaf extracts: characterization of its antioxidant and cytotoxicity activities. Surf Interfaces. 2020; 21:100799.
Rajeshkumar S, Menon S, Kumar SV, Tambuwala MM, Bakshi HA, Mehta M, Dua K. Antibacterial and antioxidant potential of biosynthesized copper nanoparticles mediated through Cissus arnotiana plant extract. J. Photochem Photobiol B. 2019; 197:111531.
John MS, Nagoth JA, Zannotti M, Giovannetti R, Mancini A, Ramasamy KP, Pucciarelli S. Biogenic synthesis of copper nanoparticles using bacterial strains isolated from an Antarctic consortium associated to a psychrophilic marine ciliate: characterization and potential application as antimicrobial agents. Mar Drugs. 2021; 19(5):263.
Rafique MA, Kiran S, Jamal A, Anjum MN, Jalal F, Munir B, Arshad MY. Green synthesis of copper nanoparticles using Allium cepa (onion) peels for removal of Disperse Yellow 3 dye. Desalin Water Treat. 2022; 272:259-65.
Kenneth M, Nwodo CR. Green synthesis of copper oxide nanoparticles using the outer layer of Allium cepa L. and evaluation of its antimicrobial properties. GSC Biol Pharm Sci. 2023; 22(1):302-311.
Hajizadeh YS, Harzandi N, Babapour E, Yazdanian M, Ranjbar R. Green synthesis and characterization of copper nanoparticles using Iranian propolis extracts. Adv. Mater Sci. Eng. 2022; 2022:8100440.
Ali SG, Haseen U, Jalal M, Khan RA, Alsalme A, Ahmad H, Khan HM. Green synthesis of copper oxide nanoparticles from the leaves of Aegle marmelos and their antimicrobial activity and photocatalytic activities. Molecules. 2023; 28(22):7499.
Nzilu DM, Madivoli ES, Makhanu DS, Wanakai SI, Kiprono GK, Kareru PG. Green synthesis of copper oxide nanoparticles and its efficiency in degradation of rifampicin antibiotic. Sci Rep. 2023; 13(1):14030.
Achamo T, Zereffa EA, Murthy HA, Ramachandran VP, Balachandran R. Phyto-mediated synthesis of copper oxide nanoparticles using Artemisia abyssinica leaf extract and its antioxidant, antimicrobial and DNA binding activities. Green Chem Lett Rev. 2022; 15(3):598-614.
Amaliyah S, Pangesti DP, Masruri M, Sabarudin A, Sumitro SB. Green synthesis and characterization of copper nanoparticles using Piper retrofractum Vahl extract as bioreductor and capping agent. Heliyon. 2020; 6(8):e04636.
Renuga D, Jeyasundari J, Athithan AS, Jacob YBA. Synthesis and characterization of copper oxide nanoparticles using Brassica oleracea var. italic extract for its antifungal application. Mater Res Express. 2020; 7(4):045007.
Abdullah HSTSH, Asseri SNARM, Mohamad WNKW, Kan SY, Azmi AA, Julius FSY, Chia PW. Green synthesis, characterization and applications of silver nanoparticle mediated by the aqueous extract of red onion peel. Environ Pollut. 2021; 271: 116295. https://doi.org/10.1016/j.envpol.2020.116295
Sinha A, Cha BG, Kim J. Three-dimensional macroporous alginate scaffolds embedded with akaganeite nanorods for the filter-based high-speed preparation of arsenic-free drinking water. ACS Appl Nano Mater. 2018; 1: 1940–1948. https://doi.org/10.1021/acsanm.8b00389
Abdelghany TM, Al-Rajhi AMH, Al Abboud MA. Recent advances in green synthesis of silver nanoparticles and their applications: About future directions. Bionanoscience. 2018; 8: 5-16. https://doi.org/10.1007/s12668-017-0413-3
Gour A, Jain NK. Advances in green synthesis of nanoparticles. Artif Cells Nanomed Biotechnol. 2019; 47(1): 844-851. https://doi.org/10.1080/21691401.2019.1577878
Yin SJ, Zhang L, Wan J. Metabolic responses and arginine kinase expression of juvenile cuttlefish (Sepia pharaonis) under salinity stress. Int J Biol Macromol. 2018; 113: 881-888. https://doi.org/10.1016/j.ijbiomac.2018.03.036
Samuel MS, Sivaramakrishna A, Mehta A. Degradation and detoxification of aflatoxin B1 by Pseudomonas putida. Int Biodeterior Biodegrad. 2014; 86: 202-209. https://doi.org/10.1016/j.ibiod.2013.08.026
Akinniyi JN. Transforming agricultural food waste in Nigeria into sustainable nanoparticles: a revolution in green nanotechnology: a mini review. Open J. of Agric Sci. 2023; 4(2): 29-53. https://doi.org/10.52417/ojas.v4i2.750.