The comparison of antioxidant properties between frozen and freeze-dried strawberries (Fragaria x ananassa) Tropical Journal of Natural Product Research
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Abstract
Strawberries (Fragaria x ananassa) are a delectable fruit renowned for their numerous health benefits, due to their high concentrations of active compounds, including antioxidants, vitamin C, phenolic acids, and flavonoids. However, the phytochemical composition of strawberries is vulnerable to degradation during post-harvest storage and food preparation. Both freezing and freeze-drying are commonly used techniques in strawberry processing, but their effects on nutritional quality and antioxidant activity have not been thoroughly studied. This study aims to investigate the nutritional compounds of fresh strawberries and compare the physical quality and antioxidant activity of frozen and freeze-dried strawberries. An observational analytical design was used. Chemical profiling using LC-MS was conducted to identify major antioxidant compounds, while also comparing changes in colour, texture, and weight between frozen and freeze-dried strawberries. Additionally, antioxidant activity was measured using the DPPH free radical scavenging assay, and the levels of total tannins, flavonoids, and polyphenols were quantified in both frozen and freeze-dried strawberries. The results showed that 88 phytochemical compounds were found in fresh strawberries, with kaempferol and quercetin being the dominant antioxidants. Freeze-dried strawberries had better physical qualities, including a brighter colour and crunchier texture, but they lost more weight compared to frozen strawberries. The freeze-drying technique also improved antioxidant capacity, as shown by lower IC50 values and higher levels of tannins, flavonoids, and polyphenols compared to frozen technique. This study implies that the freeze-drying technique is a promising method for preserving the nutritional and health-promoting properties of strawberries in post-harvest periods.
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References
1. World Population Review. Strawberry Production by Country 2024. [Online]. 2024. [cited 2024 Dec 20]. Available from: https://worldpopulationreview.com/country-rankings/strawberry-production-by-country
2. BPS Statistics Indonesia. Fruit Crop Production, 2021-2023. [Online]. 2024. [cited 2024 Dec 20]. Available from: https://www.bps.go.id/id/publication/2023/02/28/18018f9896f09f03580a614b/statistik-indonesia-2023.html
3. Chatham LA, Howard JE, Juvik JA. A natural colorant system from corn: Flavone-anthocyanin copigmentation for altered hues and improved shelf life. Food Chem. 2020;310: 125734. doi:10.1016/j.foodchem.2019.125734
4. Giampieri F, Tulipani S, Alvarez-Suarez JM, Quiles JL, Mezzetti B, Battino M. The strawberry: composition, nutritional quality, and impact on human health. Nutr. 2012;28(1): 9-19. doi:10.1016/j.nut.2011.08.009
5. Gasparrini M, Forbes-Hernandez TY, Giampieri F, Afrin S, Mezzetti B, Quiles JL, Bompadre S, Battino M. Protective effect of strawberry extract against inflammatory stress induced in human dermal fibroblasts. Mol. 2017;22(1): 164. doi:10.3390/molecules22010164
6. Mallhi IY, Sohaib M, Khan AU, Rabbani I. Antidiabetic, Antioxidative and Antihyperlipidemic Effects of Strawberry Fruit Extract in Alloxan-Induced Diabetic Rats. Foods. 2023;12(15): 2911. doi:10.3390/foods12152911
7. Ansary J, Forbes-Hernandez TY, Regolo L, Cianciosi D, Giampieri F. The anticancer activity of strawberry. Acta Hortic. 2021;1309: 925-932. doi:10.17660/ActaHortic.2021.1309.132
8. Silva FL da, Escribano-Bailon MT, Alonso JJP, Rivas-Gonzalo JC, Santos-Buelga C. Anthocyanin pigments in strawberry. LWT - Food Sci Technol. 2007;40(2): 374-382. doi:10.1016/j.lwt.2005.09.018
9. Marques KK, Renfroe MH, Brevard PBB, Lee RE, Gloeckner JW. Differences in antioxidant levels of fresh, frozen and freeze-dried strawberries and strawberry jam. Int J Food Sci Nutr. 2010;61(8): 759-769. doi:10.3109/09637481003796306
10. Afrin S, Gasparrini M, Forbes-Hernandez TY, Reboredo-Rodriguez P, Mezzetti B, Varela-López A, Giampieri F, Battino M. Promising health benefits of the strawberry: a focus on clinical studies. J Agric Food Chem. 2016;64(22): 4435-4449. doi:10.1021/acs.jafc.6b00857
11. Khoo HE, Azlan A, Tang ST, Lim SM. Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food Nutr Res. 2017;61(1): 1361779. doi:10.1080/16546628.2017.1361779
12. Basu A, Fu DX, Wilkinson M, Simmons B, Wu M, Betts NM, Du M, Lyons TJ. Strawberries decrease atherosclerotic markers in subjects with metabolic syndrome. Nutr Res,. 2010;30(7): 462-469. doi:10.1016/j.nutres.2010.06.016
13. Nowak D, Jakubczyk E. The Freeze-Drying of Foods—The Characteristic of the Process Course and the Effect of Its Parameters on the Physical Properties of Food Materials. Foods. 2020;9(10): 1488. doi:10.3390/foods9101488
14. Grover Y, Negi PS. Recent developments in freezing of fruits and vegetables: Striving for controlled ice nucleation and crystallization with enhanced freezing rates. J Food Sci. 2023;88(12): 4799-4826. doi:10.1111/1750-3841.16810
15. Ansar, Nazaruddin, Azis AD. New frozen product development from strawberries (Fragaria x ananassa Duch.). Heliyon. 2020;6(9): e05118. doi:10.1016/j.heliyon.2020.e05118
16. Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, Pandey RP, Chang CM. Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa. Mol. 2022;27(4): 1326. doi:10.3390/molecules27041326
17. Soares LAL, Bassani VL, González Ortega G, Petrovick PR. Total Flavonoid Determination for the Quality Control of Aqueous Extractives from Phyllanthus niruri L. Acta Farm Bonaerense. 2003;22(3):203-207.
18. Kang WY, Li CF, Liu YX. Antioxidant phenolic compounds and flavonoids of Mitragyna rotundifolia (Roxb.) Kuntze in vitro. Med Chem Res. 2010;19(9):1222-1232. doi:10.1007/s00044-009-9265-x
19. De Souza VR, Pereira PAP, Da Silva TLT, De Oliveira Lima LC, Pio R, Queiroz F. Determination of the bioactive compounds, antioxidant activity and chemical composition of Brazilian blackberry, red raspberry, strawberry, blueberry and sweet cherry fruits. Food Chem. 2014;156: 362-368. doi:10.1016/j.foodchem.2014.01.125
20. Seeram NP, Lee R, Scheuller HS, Heber D. Identification of phenolic compounds in strawberries by liquid chromatography electrospray ionization mass spectroscopy. Food Chem. 2006;97(1): 1-11. doi:10.1016/j.foodchem.2005.02.047
21. Putri RM, Jumeri, Falah MAF. Effects of osmotic dehydration pre-treatment on process and quality of freeze-dried strawberries (Fragaria x ananassa var. Mencir). IOP Conf Ser Earth Environ Sci. 2023;1200(1): 012030. doi:10.1088/1755-1315/1200/1/012030
22. Neri L, Faieta M, Di Mattia C, Sacchetti G, Mastrocola D, Pittia P. Antioxidant activity in frozen plant foods: effect of cryoprotectants, freezing process and frozen storage. Foods. 2020;9(12):1886. doi:10.3390/foods9121886
23. Jia X, Luo X, Nakako K, Takahisa N. Effects of moisture content and storage method on the physical properties of dried persimmon during frozen storage. Ital J Food Sci. 2024;36(3):141-150. doi:10.15586/ijfs.v36i3.2525
24. Raj R, Cosgun A, Kulić D. Strawberry water content estimation and ripeness classification using hyperspectral sensing. Agron. 2022;12(2): 425. doi:10.3390/agronomy12020425
25. Scherer R, Godoy HT. Antioxidant activity index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method. Food Chem. 2009;112(3): 654-658. doi:10.1016/j.foodchem.2008.06.026
26. Rufino MSM, Fernandes FAN, Alves RE, de Brito ES. Free radical-scavenging behaviour of some north-east Brazilian fruits in a DPPH. system. Food Chem. 2009;114(2): 693-695. doi:10.1016/j.foodchem.2008.09.098
27. Las DW, Fabiana G da SP, Mayara CS, Juliete G de L de S, Willian F da C, Camila WL. Chemical composition and antimicrobial and antioxidant activity of essential oil and various plant extracts from Prunus myrtifolia (L.) Urb. Afr J Agric Res. 2014;9(9): 846-853. doi:10.5897/AJAR2013.8260
28. Olugbami JO, Gbadegesin MA, Odunola OA. In vitro evaluation of the antioxidant potential, phenolic and flavonoid contents of the stem bark ethanol extract of Anogeissus leiocarpus. Afr J Med Med Sci. 2014;43(Suppl 1):101-109.
29. Indriaty I, Ginting B, Hasballah K, Djufri D. A comparative study of total tannin contents and antimicrobial activities in methanol extracts of Rhizophoraceae species. Heca J Appl Sci. 2023;1(2): 62-70. doi:10.60084/hjas.v1i2.89
30. Antasionasti I, Datu OS, Lestari US, Abdullah SS, Jayanto I. Correlation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white. Borneo J Pharm. 2021;4(4):301-310.
doi:10.33084/bjop.v4i4.2497
31. Mphahlele RR, Fawole OA, Makunga NP, Opara UL. Effect of drying on the bioactive compounds, antioxidant, antibacterial and antityrosinase activities of pomegranate peel. BMC Complement Altern Med. 2016;16(1): 143. doi:10.1186/s12906-016-1132-y
32. Ullah A, Munir S, Badshah SL, Khan N, Ghani L, Poulson BG, Emwas AH, Jaremko M. Important Flavonoids and Their Role as a Therapeutic Agent. Mol. 2020;25(22): 5243. doi:10.3390/molecules25225243
33. Ahlem Rebaya, Souad Igueld Belghith, Béatrice Baghdikian, Valérie Mahiou Leddet, Fathi Mabrouki, Evelyne Olivier, Jamila kalthoum Cherif, Malika Trabelsi Ayadi. Total Phenolic, Total Flavonoid, Tannin Content, and Antioxidant Capacity of Halimium halimifolium (Cistaceae). J Appl
Pharm Sci. Published online January 30, 2015. doi:10.7324/JAPS.2015.50110
34. Pérez-Gregorio MR, Regueiro J, González-Barreiro C, Rial-Otero R, Simal-Gándara J. Changes in antioxidant flavonoids during freeze-drying of red onions and subsequent storage. Food Control. 2011;22(7): 1108-1113. doi:10.1016/j.foodcont.2011.01.006
35. Rababah TM, Al-U’datt MH, Angor M, Gammoh S, Abweni F, Magableh G, Almajwal A, Yücel S, AL-Rayyan Y, AL-Rayyan N. Effect of Drying and Freezing on the Phytochemical Properties of Okra during Storage. ACS Omega. 2023;8(38): 34448-34457. doi:10.1021/acsomega.3c02560
36. Borges JM, Lucarini M, Durazzo A, Rufino Arcanjo DD, Rodrigues Lima SK, da Silva RA. Effect of freezing and freeze-drying on bioactive compounds and antioxidant activity of carnauba pulp (Copernicia prunifera (Mill.) H.E. Moore). MOJ Food Process. Technol. 2023;11(2):78-82. doi:10.15406/mojfpt.2023.11.00284
37. Ozay-Arancioglu I, Bekiroglu H, Karadag A, Saroglu O, Tekin-Çakmak ZH, Karasu S. Effect of different drying methods on the bioactive, microstructural, and in-vitro bioaccessibility of bioactive compounds of the pomegranate arils. Food Sci. Technol. 2022;42: 1-9. doi:10.1590/fst.06221