Comprehensive Quality Assessment of Dittrichia viscosa (L.) from Southeast Morocco: Physicochemical Properties, Phytochemical Composition, Microbiological Profile and Mineral Content Analysis Tropical Journal of Natural Product Research
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Abstract
Dittrichia viscosa (L.) Greuter, is a perennial herb renowned for its therapeutic properties. The present study aimed to carry out a comprehensive assessment of the quality of D. viscosa aerial parts. The aerial parts of D. viscosa were sourced from the Ouarzazate region in southeast Morocco. Dried powdered D. viscosa aerial parts were extracted using aqueous ethanol, and cyclohexane by various extraction techniques. Essential oil was obtained by hydrodistillation in a Clevenger-type apparatus. The quality of the plant material, including its essential oil was assessed by evaluating various parameters including extraction yields, organoleptic and physicochemical properties, phytochemical composition, mineral content, and microbiological profile. Results showed that the yields of the essential oil and other extracts from D. viscosa aerial parts significantly depends on the extraction solvents and techniques used. The hydroethanol extract produced the highest levels of total phenol and total tannin contents, whereas the cyclohexane extract showed the highest total flavonoid content. The organoleptic analysis indicated a slimy, dark yellow oil, with an herbaceous camphoraceous odour The essential oil was predominantly composed of sesquiterpenes, including γ-Muurolene, α-Muurolene, and β-Cadinene. The quality assessment indices and physicochemical properties suggested a commendable quality of the oil, with properties comparable to or exceeding those of other species within the Asteraceae family. Microbiological analysis showed that D. viscosa conformed to WHO standards for herbal medicines, which suggests that the plant is safe for consumption. However, the mineral analysis showed elevated cadmium level, hence the need for caution in its medicinal use.
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References
Boulos L. Medicinal plants of North Africa. Medicinal plants of North Africa. 1983.
Brullo S, de Marco G. Taxonomical revision of the genus Dittrichia (Asteraceae). Port Acta Biol. 2000; 19(1):341-54.
Mssillou I, Agour A, Allali A, Saghrouchni H, Bourhia M, El Moussaoui A, et al. Antioxidant, Antimicrobial, and Insecticidal Properties of a Chemically Characterized Essential Oil from the Leaves of Dittrichia viscosa L. Molecules. 2022; 27(7):2282.
Madani L, Derriche R, Haoui IE. Essential oil of Algerian Inula viscosa leaves. J Essent Oil-Bear Plants. 2014; 17(1):164-8.
Haoui IE, Derriche R, Madani L, Oukali Z. Analysis of the chemical composition of essential oil from Algerian Inula viscosa (L.) Aiton. Arab J Chem. 2015; 8(4):587-90.
Jerada R, Er-Rakibi A, Cherkani Hassani A, Benzeid H, El Ouardi A, Harhar H, et al. A comprehensive review on ethnomedicinal uses, phytochemistry, toxicology, and pharmacological activities of Dittrichia viscosa (L.) Greuter. J Tradit Complement Med. 2024.
AFNOR. NF EN ISO 712 Cereals and cereal products - Determination of moisture content - Routine reference method. AFNOR Editions; 2010.
AFNOR. NF EN ISO 2171 Cereals, pulses and by-products - Determination of ash yield by incineration.: AFNOR Editions; 2010.
Horwitz W, Latimer GW. Official methods of analysis of the Association of Official Analytical Chemists. . 18 ed. Gaithersburg: AOAC International; 2005.
Lecoq R. Manuel d'analyses alimentaires et d'expertises usuelles. 1965.
WHO. Quality control methods for medicinal plant materials: World Health Organization; 1998.
Dorsaf BH, Hanen BI, Chokri J, Larbi KM, Manef A. Chemical composition of some Tunisian Eucalyptus essential oils as obtained by hydrodistillation using Clevenger type apparatus. Biosci Biotechnol Res Asia. 2016; 7(2):647-56.
ISO. NF ISO 1242 Essential oils - Determination of acid value by two titration methods, manual and automatic.: AFNOR Editions; 2023.
ISO. ISO 3657:2023 Animal and vegetable fats and oils Determination of saponification value. 2023.
ISO. ISO 706:2001 Essential Oils - Determination of Ester Value. 2001.
ISO. ISO 3961:2018 Animal and vegetable fats and oils Determination of iodine value. 2018.
ISO. NF ISO 279 Essential oils. Determination of relative density at 20 °C — Reference method. AFNOR Editions; 1999.
ISO. NF EN ISO 6320 Animal and vegetable fats and oils - Determination of refractive index. AFNOR Editions; 2017.
Boukhatem MN, Amine FM, Kameli A, Saidi F, Walid K, Mohamed SB. Quality assessment of the essential oil from Eucalyptus globulus Labill of Blida (Algeria) origin. Int Lett Chem Phys Astron. 2014; 17(3):303-15.
Poh-Hwa T, Yoke-Kqueen C, Indu Bala J, Son R. Bioprotective properties of three Malaysia Phyllanthus species: An investigation of the antioxidant and antimicrobial activities. Int Food Res J. 2011; 18(3).
Ordonez A, Gomez J, Vattuone M. Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chem. 2006; 97(3):452-8.
Julkunen-Tiitto R. Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics. J Agric Food Chem. 1985;
33(2):213-7.
AOCS. AOCS (ed) Official methods & recommended practices of the American Oil Chemists Society. Champaign, IL1993.
Paul B, Chanda S, Das S, Singh P, Pandey B, Giri S. Mineral assay in atomic absorption spectroscopy. The beats nat sci. 2014; 4(1):1-17.
ISO. ISO 6887-1:2017 Microbiology of the food chain Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 1: General rules for the preparation of the initial suspension and decimal dilutions2017.
ISO. ISO 4833-1:2013 Microbiology of the food chain Horizontal method for the enumeration of microorganisms Part 1: Colony count at 30 °C by
the pour plate technique 2013.
AFNOR. NF V 08-050 Microbiology of food and animal feeding stuffs - Enumeration of presumptive coliforms by colony-count technique at 30 °C. AFNOR Editions; 2009.
ISO. ISO 6888-1:2021 Microbiology of the food chain Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species). Part 1: Method using Baird-Parker agar medium2021.
ISO. ISO 6579-1:2017 Microbiology of the food chain Horizontal method for the detection, enumeration and serotyping of Salmonella. Part 1: Detection of Salmonella spp2017.
ISO. ISO 7932:2004 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of presumptive Bacillus cereus. Colony-count technique at 30 degrees C2004.
ISO. ISO 15213-2:2023 Microbiology of the food chain Horizontal method for the detection and enumeration of Clostridium spp. Part 2: Enumeration of Clostridium perfringens by colony-count technique2023.
ISO. NF V08-059 Microbiology of food animal feeding stuffs - Enumeration of yeasts and moulds by colony-count technique at 25 °C - Routine method. AFNOR Editions; 2002.
Aissa I, Nimbarte VD, Zardi‐Bergaoui A, Znati M, Flamini G, Ascrizzi R, et al. Isocostic acid, a promising bioactive agent from the essential oil of Inula viscosa (L.): Insights from drug likeness properties, molecular docking and SAR analysis. Chem Biodivers. 2019; 16(4):e1800648.
Gharred N, Dbeibia A, Falconieri D, Hammami S, Piras A, Dridi-Dhaouadi S. Chemical composition, antibacterial and antioxidant activities of essential oils from flowers, leaves and aerial parts of Tunisian Dittrichia Viscosa. J Essent Oil Res. 2019; 31(6):582-9.
Ounoughi A, Ramdani M, Lograda T, Chalard P. Chemotypes and antibacterial activities of Inula viscosa essential oils from Algeria. Biodiversitas. 2020; 21(4).
Nadia B, Mesli F, Zahra BF, Merad-Boussalah N, Radja A, Muselli A, et al. Chemical composition variability and vascular endothelial growth factor receptors inhibitory activity of Inulaviscosa essential oils from Algeria. J Biomol Struct Dyn. 2020:1-19.
Qneibi M, Hanania M, Jaradat N, Emwas N, Radwan S. Inula viscosa (L.) Greuter, phytochemical composition, antioxidant, total phenolic content, total flavonoids content and neuroprotective effects. Eur J Integr Med. 2021; 42:101291.
Ounaissia K. Total Phenol Content, Antioxidant And Anti-Inflammatory Activities Of Inula Viscosa From Guelma- Algeria. Int J Agric Nat Sci. 2021; 14(3):186-93.
Mssillou I, Agour A, Slighoua M, Tourabi M, Nouioura G, Lyoussi B, et al. Phytochemical characterization, antioxidant activity, and in vitro investigation of antimicrobial potential of Dittrichia viscosa L. leaf extracts against nosocomial infections. Acta Ecol Sin. 2021.
Truong D-H, Nguyen DH, Ta NTA, Bui AV, Do TH, Nguyen HC. Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of Severinia buxifolia. J Food Qual. 2019; 2019.
Hayouni EA, Abedrabba M, Bouix M, Hamdi M. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chem. 2007; 105(3):1126-34.
Katkade M, Syed H, Andhale R, Sontakke M. Fatty acid profile and quality assessment of safflower (Carthamus tinctorius) oil at region. J Pharmacogn Phytochem. 2018; 7(2):3581-5.
Zekri N, Elazzouzi H, Ailli A, Gouruch AA, Radi FZ, El Belghiti MA, et al. Physicochemical Characterization and Antioxidant Properties of Essential
Oils of M. pulegium (L.), M. suaveolens (Ehrh.) and M. spicata (L.) from Moroccan Middle-Atlas. Foods. 2023; 12(4):760.
Ghiasy-Oskoee M, AghaAlikhani M. Towards utilizing Asteraceae alternative oilseed species on marginal lands: Agronomic performance, fatty acid composition, oil biocompounds, and oil physicochemical properties of Asteraceae species. J Agric Food Res. 2023; 14:100799.
Khaliq HA, Chaudhary BA. Pharmacognostic and phytochemical studies on Parthenium hysterophorus L. J Biomed Pharm Res. 2016; 5(1):65-75.
Suryawanshi VR, Yadav HR, Surani HC. Pharmacognostic and phytochemicals evaluation of Blumea species. Phytochemistry. 2021; 26:2079-83.
Asraoui F, Kounnoun A, Cacciola F, El Mansouri F, Kabach I, Oulad El Majdoub Y, et al. Phytochemical profile, antioxidant capacity, α-amylase and α-glucosidase inhibitory potential of wild Moroccan inula viscosa (L.) aiton leaves. Molecules. 2021; 26(11):3134.
Gökbulut A, Özhana O, Satılmiş B, Batçioğlu K, Günal S, Şarer E. Antioxidant and antimicrobial activities, and phenolic compounds of selected Inula species from Turkey. Nat Prod Commun. 2013; 8(4):1934578X1300800417.
Mahmoudi H, Hosni K, Zaouali W, Amri I, Zargouni H, Hamida NB, et al. Comprehensive phytochemical analysis, antioxidant and antifungal activities of Inula viscosa aiton leaves. J Food Saf. 2016; 36(1):77-88.
Rhimi W, Ben Salem I, Immediato D, Saidi M, Boulila A, Cafarchia C. Chemical composition, antibacterial and antifungal activities of crude Dittrichia viscosa (L.) greuter leaf extracts. Molecules. 2017; 22(7):942.
Salim H, Rimawi WH, Mjahed A. Analysis of extracts from Palestinian Inula viscosa for their phenolic, flavonoid and lipid contents, antioxidant and antibacterial activity. J Chem Biochem. 2017.
El Ouariachi E, Bouyanzer A. Antioxidant activity of solvent extracts of Inula viscosa from Morocco. Arabian Journal of Chemical and Environmental Research. 2014; 1(1):33-40.
Ma G-H, Chen K-X, Zhang L-Q, Li Y-M. Advance in biological activities of natural guaiane-type sesquiterpenes. Med Chem Res. 2019; 28(9):1339-58.
Cox-Georgian D, Ramadoss N, Dona C, Basu C. Therapeutic and Medicinal Uses of Terpenes. In: Joshee N, Dhekney SA, Parajuli P, editors. Medicinal Plants: From Farm to Pharmacy. Cham: Springer International Publishing; 2019. p. 333-59.
WHO. Trace elements in human nutrition and health: World Health Organization; 1996.
WHO. WHO guidelines for assessing quality of herbal medicines with reference to contaminants and residues: World Health Organization; 2007.
Mrid RB, Bouchmaa N, Kabach I, Zouaoui Z, Chtibi H, Maadoudi ME, et al. Dittrichia viscosa L. Leaves: A Valuable Source of Bioactive Compounds with Multiple Pharmacological Effects. Molecules. 2022; 27(7):2108.
Buscaroli A, Zannoni D, Menichetti M, Dinelli E. Assessment of metal accumulation capacity of Dittrichia viscosa (L.) Greuter in two different Italian mine areas for contaminated soils remediation. J Geochem Explor. 2017; 182:123-31.
Martínez-Sánchez MJ, García-Lorenzo ML, Pérez-Sirvent C, Bech J. Trace element accumulation in plants from an aridic area affected by mining activities. J Geochem Explor. 2012; 123:8-12.
Cicero N, Gervasi T, Durazzo A, Lucarini M, Macrì A, Nava V, et al. Mineral and Microbiological Analysis of Spices and Aromatic Herbs. Foods. 2022; 11(4):548.