Optimization and Characterization of Dayak Onion (Eleutherine palmifolia (L.) Merr) Extract Nanoparticles Using Cross-link Method Tropical Journal of Natural Product Research
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Abstract
Dayak onions contain 1,4-Naphtoquinone, which has been shown to possess anticancer activity. Dayak onion extract nanoparticle was formulated to improve its bioavailability using ionic gelation. This research aims to formulate and characterise Dayak onion extract nanoparticles (NPs). The organoleptic properties, pH, entrapment efficiency, particle size, SEM and XRD morphology, and chemical properties of the NPs were investigated by FTIR and DSC. The results showed no aggregation or phase separation; the pH of the formulated NPs was 3.56 ± 0.00. The resulting particle size was 329 ± 87.0 nm, while the entrapment efficiency was 94.84 ± 0.00%. Based on SEM and XRD evaluation, the results obtained were heterogeneous with a non-uniform surface, and the particle structure was amorphous with a degree of crystallinity of 37.98%. Meanwhile, the results of the chemical properties tests using FTIR and DSC agreed with previous studies. There was a shift of functional groups in the IR spectra of the nanoparticles and a shift of melting point in the DSC thermogram, which indicated that Dayak onion extract nanoparticles were successfully formed. Based on data obtained in this study, we conclude that Dayak onion extract can be formulated into nanoparticles with efficient characteristics.
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Pakki E, Sumarheni FA, Ismail, Safirahidzni S. Nanoparticle Formulation of Dayak Onion Extract (Eleutherine americana (Aubl) Merr) with Varying Concentrations of Chitosan-Tripolyphosphate (TPP). J Trop Pharm Chem 2016;3(4): 251–263.
Galing RY. Dayak onion (Eleutherine palmifolia) as a multifunctional medicinal plant. Res Dev News 2009;15(3): 16–18.
Hanutami B, Budiman A. Use of Nano Technology in Herbal Medicine Formulation. Pharmaca 2017;15: 29–39.
Abdassah M. Nanoparticles with Ionic Gelation. J Farmaka 2017;15(1): 45–52.
Abdelwahed W, Degobert G, Stainmesse S, Fessi H. Freeze-drying of Nanoparticles: Formulation, Process and Storage Considerations. Adv Drug Deliv Rev 2006;58(15): 1688–1713.
Handaratri A. Yuniati Y. Study of Anthocyanin Extraction from Mulberry Fruit using the Microwave Sonication Method. J Sci Civ Eng Chem 2017;4(1): 63-67.
Sari R, Paramanana A, Isadiartuti D, Rahayyu AM. Effect of Polymer Amount on Physical Characteristics of Diterpene Lactone-Chitosan Diterpene Fraction Nanoparticle Release. J Pharm and Clin Sci 2020;7(2): 99-106.
Iswandana R, Putri KSS, Dwiputra R, Yanuari T, Sari SP, Djajadisastra J. Formulation of Chitosan Tripolyphosphate-Tentrandine Beads Using Ionic Gelation Method: In Vitro and Vivo Evaluation. Int J Appl Pharm. 2017;9(5): 109-115.
Purnama EF, Enjoyn S, Langenati R. Effect of Reaction Temperature on the Degree of Crystallinity and Composition of Hydroxyapatite Made Using Water and Artificial Body Fluid. J Material Sci Ind 2020: 154-159.
Efiana NA, Nugroho AK, Martien R. Formulation of Losartan Nanoparticles with Chitosan as a Carrier. J Pharma Sci Ind, 2013;11(1) 7–12.
Budastra WCG, Hajrin W, Gita WD. Effect of Stirring Speed on the Characteristics of Juwet Fruit Juice Nanoparticles (Syzygium cumini L.). J Med Unram, 2022;11(3): 1000–1006.
Laili H, Winarti L, Sari LOR. Preparation and Characterisation of Chitosan-Naringenin Nanoparticles with Varying Mass Ratios of Chitosan-Sodium Tripolyphosphate. E-J Health Library 2014; 2(2): 308–313.
Mutiah R, Ardania W, Suryadinata A, Megawati DS, Listiyana A, Wafi A, Annisa R. Influence of Chitosan Concentration on Characteristics of Microspheres Delivery System Prepared from Eleutherine palmifolia (L.) Merr. Extract. Pharmacog J 2022;14(6): 702-709.
Fadilah F. Differential Scanning Calorimeter Profiling for Determining Nanoparticles Ethanol Extract of Clove (Syzygium aromaticum L.) toward Michigan Cancer Foundation-7 Human Breast Cancer Cell Lines. Asian J Pharma. 2014;12(4): 1452-1455.
Koshy O, Subramanian L, Thomas S. Chapter 5: Differential Scanning Calorimetry in Nanoscience and Nanotechnology. Elsevier; 2017: 109-122
Alehosseini E, Tabarestani HS, Kharazmi MS, Jafari SM. Physicochemical, Thermal, and Morphological Properties of Chitosan Nanoparticles Produced by Ionic Gelation. Foods 2022; 11(3841): 1-14.
Martien R. Technology Developments Nanoparticles as Drugs. Pharmaceutics Magazine 2012:8(1): 133–144.
Anugraini A, Syahbanu I, Melati HA. Effect of Sonication Time on the Characteristics of Cellulose Acetate Synthesized from Areca Fiber. J Equator Chem 2018;7(3): 250-257.
Pradana AT, Nawatila R, Rachman MR. Physical Characteristics of Quercetin Microparticles with a Combination of Chitosan-Sodium Tripolyphosphate Using the Orifice Ionic Gelation Method. Ibn Sina Scientific J 2022;7(1): 133-142.
Patel JK, Patel RP, Amin AF, Patel MM. Formulation and Evaluation of Mucoadhesive Glipizide Microspheres. AAPS PharmSciTech 2005;6(1): E49-E55.
Fransira I, Anggreini AF, Yanuhar U, Maftuch. Antibacterial Activity of Dayak Onion Bulbs (Eleutherine palmifolia (L.) Mer ) Ethanol Fraction against Pseudomonas fluorescens and Its Secondary Metabolite Analysis. Research J Life Sci 2019;6(2): 94–103.