Renal Function Indices of Ulcerated Rats Pre-Treated with Binary Combinations of Persea americana Seed and Bryophyllum pinnatum Leaf Ethyl Acetate Fraction

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Published: Dec 29, 2024
DOI: https://doi.org/10.26538/tjnpr/v8i12.47
Keywords:
Nephrotoxicity, Gastric ulcer, Indomethacin, Bryophyllum pinnatum, Persea americana

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Emeka S. Asiwe
Department of Biological Science, University of Agriculture and Environmental Sciences Umuagwo, Imo State, Nigeria
Uchechi E. Enenebeaku
Department of Biological Science, University of Agriculture and Environmental Sciences Umuagwo, Imo State, Nigeria
Precious N. Alisi
Faculty of Medical Laboratory Science, Federal University Otuoke, Bayelsa State, Nigeria
Majesty K. C. Duru
Department of Biological Science, University of Agriculture and Environmental Sciences Umuagwo, Imo State, Nigeria
Obinna C. U. Adumanya
Department of Biological Science, University of Agriculture and Environmental Sciences Umuagwo, Imo State, Nigeria
Adaku V. Iwueke
Department of Biological Science, University of Agriculture and Environmental Sciences Umuagwo, Imo State, Nigeria

Abstract

The use of certain medications and herbal remedies poses a significant risk to renal function. The study aimed to investigate the effect of binary combinations of Persea americana seed (PAEF) and Bryophyllum pinnatum leaf (BPEF) ethyl acetate fractions on renal function indices of ulcerated rats. Wistar rats were divided into ten groups of five rats each. The groups were designated: normal control (NC) (Group 1), ulcer control (UC) (Group 2), Omeprazole (OMEP) (Group 3), PAEF (Group 4), BPEF (Group 5), PAEF + BPEF (1:1) (Group 6), PAEF + BPEF (1:2) (Group 7), PAEF + BPEF (1:3) (Group 8), PAEF + BPEF (2:1) (Group 9), and PAEF + BPEF (3:1) (Group 10). Groups 4-10 were pre-treated with 400 mgkg-1 body weight/day of ethyl acetate fractions of the plants, and their binary combinations orally for 21 days. On the 22nd day, after 12 h fasting, gastric ulcer was induced in the rats in groups 2-10 by a single oral dose of indomethacin (30 mg/kg body weight). Results showed that the administration of PAEF + BPEF (1:2), PAEF + BPEF (1:3), PAEF + BPEF (2:1), PAEF + BPEF (3:1), and omeprazole caused significant (p<0.05) elevation of serum urea when compared to NC and UC. The binary combinations PAEF + BPEF (1:1), PAEF + BPEF (1:3), PAEF + BPEF (2:1), PAEF + BPEF (3:1) caused normalization of serum calcium, magnesium, and zinc concentrations comparable to normal control. Therefore, the binary combinations of PAEF and BPEF may distort kidney function and electrolyte balance.

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Asiwe, E. S., Enenebeaku, U. E., Alisi, P. N., Duru, M. K. C., Adumanya, O. C. U., & Iwueke, A. V. (2024). Renal Function Indices of Ulcerated Rats Pre-Treated with Binary Combinations of Persea americana Seed and Bryophyllum pinnatum Leaf Ethyl Acetate Fraction. Tropical Journal of Natural Product Research (TJNPR), 8(12), 9670 – 9678. https://doi.org/10.26538/tjnpr/v8i12.47
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Vol. 8 No. 12 (2024): Tropical Journal of Natural Product Research (TJNPR)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Asiwe, E. S., Enenebeaku, U. E., Alisi, P. N., Duru, M. K. C., Adumanya, O. C. U., & Iwueke, A. V. (2024). Renal Function Indices of Ulcerated Rats Pre-Treated with Binary Combinations of Persea americana Seed and Bryophyllum pinnatum Leaf Ethyl Acetate Fraction. Tropical Journal of Natural Product Research (TJNPR), 8(12), 9670 – 9678. https://doi.org/10.26538/tjnpr/v8i12.47

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