The Effect of Different Citric Acid Concentrations on Physicochemical and Antioxidant Properties of Red Pitaya Peel Gummy Candies

Main Article Content

Nur S. Mohd Isa
Hui Y. Cheok
Aliah Z. Mohsin
Nur M. MohdMaidin

Abstract

Red pitaya peels (RPP), often discarded as waste, are rich in betacyanin, making them a potential natural colourant for food products such as gummy candy. However, citric acid may influence the quality and stability of the colourant. This study examined the physicochemical, antioxidant, and sensory properties of RPP gummy candies with different citric acid concentrations. The RPP were dehydrated, ground into powder, and incorporated into gummy formulations. Citric acid significantly reduced water activity (p<0.05), lowered pH, and increased titratable acidity. Colour analysis showed a significant rise (p<0.05) in a* value with higher citric acid, indicating a redder colour. Samples containing 3% citric acid exhibited a marked reduction (p<0.05) in chewiness. Antioxidant properties were also affected, with the 3% citric acid sample showing the highest total phenolic content (0.91 mg GAE/g) and DPPH radical scavenging activity (40.73%). However, total betacyanin content declined significantly (p<0.05) in samples with citric acid, ranging from 0.55-0.85 mg/g, compared to 1.79 mg/g with 0% citric acid. Sensory evaluation revealed that the sample F2 with 1% citric acid sample received the highest scores for most attributes, suggesting an optimal balance between flavour and texture. Overall, the study highlights the potential of RPP as a natural colourant and demonstrates how varying citric acid concentrations impact the physical, antioxidant, and sensory qualities of gummy candy. These findings underscore the importance of optimising citric acid levels to maintain both the functional and sensory attributes of RPP-infused gummy candies.  

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How to Cite
Isa, N. S. M., Cheok, H. Y., Mohsin, A. Z., & MohdMaidin, N. M. (2024). The Effect of Different Citric Acid Concentrations on Physicochemical and Antioxidant Properties of Red Pitaya Peel Gummy Candies . Tropical Journal of Natural Product Research (TJNPR), 8(12), 9654 – 9663. https://doi.org/10.26538/tjnpr/v8i12.45
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Articles
Author Biographies

Nur S. Mohd Isa, Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia 

Food and Innovation Sustainability Group, Food Security Cluster, Universiti Malaysia Terengganu, Malaysia 

Aliah Z. Mohsin, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia 

Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia, Malaysia. 

Nur M. MohdMaidin, Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia 

Food and Innovation Sustainability Group, Food Security Cluster, Universiti Malaysia Terengganu, Malaysia 

How to Cite

Isa, N. S. M., Cheok, H. Y., Mohsin, A. Z., & MohdMaidin, N. M. (2024). The Effect of Different Citric Acid Concentrations on Physicochemical and Antioxidant Properties of Red Pitaya Peel Gummy Candies . Tropical Journal of Natural Product Research (TJNPR), 8(12), 9654 – 9663. https://doi.org/10.26538/tjnpr/v8i12.45

References

Luu T, Le T, Huynh N, Quintela-Alonso P. Dragon fruit: A review of health benefits and nutrients and its sustainable development under climate changes in Vietnam. Czech J Food Sci. 2021;39:71–94. https://doi.org/10.17221/139/2020-CJFS.

Hua Q, Chen C, Tel Zur N, Wang H, Wu J, Chen J, Zhang Z, Zhao J, Hu G, Qin Y. Metabolomic characterization of pitaya fruit from three red-skinned cultivars with different pulp colors. Plant Physiol Biochem. 2018;126:117–125. https://doi.org/https://doi.org/10.1016/j.plaphy.2018.02.027.

Huang Y, Brennan MA, Kasapis S, Richardson SJ, Brennan CS. Maturation Process, Nutritional Profile, Bioactivities and Utilisation in Food Products of Red Pitaya Fruits: A Review. Foods. 2021;10. https://doi.org/10.3390/foods10112862.

Soedirga L, Marchellin M. Physicochemical Properties of Jelly Candy Made with Pectin from Red Dragon Fruit Peel in Combination with Carrageenan. J Sustain Agric. 2022;37(1):1-14. https://doi.org/10.20961/carakatani.v37i1.53798.

Manihuruk F, Suryati T, Arief I. Effectiveness of the Red Dragon Fruit (Hylocereus polyrhizus) Peel Extract as the Colorant, Antioxidant, and Antimicrobial on Beef Sausage. Trop Anim Sci J. 2017;40(1):47–54. https://doi.org/10.5398/medpet.2017.40.1.47.

Madane P, Das AK, Nanda PK, Bandyopadhyay S, Jagtap P, Shewalkar A, Maity B. Dragon fruit (Hylocereus undatus) peel as antioxidant dietary fibre on quality and lipid oxidation of chicken nuggets. J Food Sci Technol. 2020;57(4):1449–1461. https://doi.org/10.1007/s13197-019-04180-z.

Esatbeyoglu T, Wagner AE, Schini-Kerth VB, Rimbach G. Betanin—A food colorant with biological activity. Mol Nutr Food Res. 2015;59(1):36–47. https://doi.org/https://doi.org/10.1002/mnfr.201400484.

Sadowska-Bartosz I, Bartosz G. Biological Properties and Applications of Betalains. Molecules. 2021;26(9):2520.

Halimfanezi L, Asra RR. A Review: Analysis of Betacyanin Levels in Various Natural Products. Asian J Pharm Res Dev. 2020;8(5):88–95. https://doi.org/10.22270/ajprd.v8i5.846.

Stintzing FC, Herbach KM, Mosshammer MR, Carle R, Yi W, Sellappan S, Akoh CC, Bunch R, Felker P. Color, Betalain Pattern, and Antioxidant Properties of Cactus Pear (Opuntia spp.) Clones. J Agric Food Chem. 2005;53(2):442–451. https://doi.org/10.1021/jf048751y.

Skalicky M, Kubes J, Shokoofeh H, Tahjib-Ul-Arif M, Vachova P, Hejnak V. Betacyanins and betaxanthins in cultivated varieties of Beta vulgaris L. compared to weed beets. Molecules. 2020;25(22):5395. https://doi.org/10.3390/molecules25225395.

Wong YM, Siow LF. Effects of heat, pH, antioxidant, agitation and light on betacyanin stability using red-fleshed dragon fruit (Hylocereus polyrhizus) juice and concentrate as models. J Food Sci Technol. 2015;52(5):3086–3092. https://doi.org/10.1007/s13197-014-1362-2.

Das M, Saeid A, Hossain MF, Jiang GH, Eun JB, Ahmed M. Influence of extraction parameters and stability of betacyanins extracted from red amaranth during storage. J Food Sci Technol. 2019;56(2):643–653. https://doi.org/10.1007/s13197-018-3519-x.

Karangutkar AV, Ananthanarayan L. Evaluating the effect of additives on stability of betacyanin pigments from Basella rubra in a model beverage system during storage. J Food Sci Technol. 2021;58:1262–1273. https://doi.org/10.1007/s13197-020-04635-8.

Herbach KM, Rohe M, Stintzing FC, Carle R. Structural and chromatic stability of purple pitaya (Hylocereus polyrhizus [Weber] Britton & Rose) betacyanins as affected by the juice matrix and selected additives. Food Res Int. 2006;39(6):667–677. https://doi.org/https://doi.org/10.1016/j.foodres.2006.01.004.

Skopińska A, Szot D, Tuwalska D, Starzak K, Osiadło M, Wybraniec S. The effect of citric acid on stabilization of betanin solutions. Chall Mod Technol. 2014;5(2):19–24.

Ge H, Wu Y, Woshnak LL, Mitmesser SH. Effects of hydrocolloids, acids and nutrients on gelatin network in gummies. Food Hydrocoll. 2021;113:106549. https://doi.org/https://doi.org/10.1016/j.foodhyd.2020.106549.

Ciriminna R, Meneguzzo F, Delisi R, Pagliaro M. Citric acid: emerging applications of key biotechnology industrial product. Chem Cent J. 2017;11:22. https://doi.org/10.1186/s13065-017-0251-y.

Oplatowska-Stachowiak M, Elliott CT. Food colors: Existing and emerging food safety concerns. Crit Rev Food Sci Nutr. 2017;57(3):524–548. https://doi.org/10.1080/10408398.2014.889652.

de Oliveira ZB, Silva da Costa DV, da Silva dos Santos AC, da Silva Júnior AQ, de Lima Silva A, de Santana RCF, Costa ICG, de Sousa Ramos SF, Padilla G, da Silva SKR. Synthetic Colors in Food: A Warning for Children’s Health. Int J Environ Res Public Health. 2024;21(6):682.

Faridah A, Holinesti R, Syukri D. Betalains from Red Pitaya Peel (Hylocereus polyrhizus): Extraction, Spectrophotometric and HPLC-DAD Identification, Bioactivity and Toxicity Screening. Pak J Nutr. 2015;14:976–982. https://doi.org/10.3923/pjn.2015.976.982.

Jiang H, Zhang W, Li X, Shu C, Jiang W, Cao J. Nutrition, phytochemical profile, bioactivities and applications in food industry of pitaya (Hylocereus spp.) peels: A comprehensive review. Trends Food Sci Technol 2021;116:199–217. https://doi.org/https://doi.org/10.1016/j.tifs.2021.06.040.

Charoen R, Savedboworn W, Phuditcharnchnakun S, Khuntaweetap T. Development of Antioxidant Gummy Jelly Candy Supplemented with Psidium guajava Leaf Extract. KMUTNB Int J Appl Sci Technol. 2015;8(2):145–151.

Bakar J, Shu CE, Muhammad SKS, Hashim DM, Noranizan A. Physico-chemical characteristics of red pitaya (Hylocereus polyrhizus) peel. Int Food Res J. 2011;18:279-286.

Ben Rejeb I, Dhen N, Kassebi S, Gargouri M. Quality Evaluation and Functional Properties of Reduced Sugar Jellies Formulated from Citrus Fruits. J Chem. 2020;2020:5476872. https://doi.org/https://doi.org/10.1155/2020/5476872.

Tarahi M, Mohamadzade Fakhr-davood M, Ghaedrahmati S, Roshanak S, Shahidi F. Physicochemical and Sensory Properties of Vegan Gummy Candies Enriched with High-Fiber Jaban Watermelon Exocarp Powder. Foods. 2023;12(7):1478. https://doi.org/10.3390/foods12071478.

Priatni S, Pradita A. Stability Study of Betacyanin Extract from Red Dragon Fruit (Hylocereus Polyrhizus) Peels. Procedia Chem. 2015;16:438–444. https://doi.org/10.1016/j.proche.2015.12.076.

Aiello F, Caputo P, Oliviero Rossi C, Restuccia D, Spizzirri UG. Formulation of Antioxidant Gummies Based on Gelatin Enriched with Citrus Fruit Peels Extract. Foods. 2024;13:320.

Okafor CE, Ijoma IK, Igboamalu CA, Ezebalu CE, Eze CF, Osita-Chikeze JC, Uzor CE, Ekwuekwe AL. Secondary metabolites, spectra characterization, and antioxidant correlation analysis of the polar and nonpolar extracts of Bryophyllum pinnatum (Lam) Oken. Biotechnol (Pozn). 2024;105(2):121-136.

Ergun R, Lietha R, Hartel RW. Moisture and Shelf Life in Sugar Confections. Crit Rev Food Sci Nutr. 2010;50:162–192. https://doi.org/10.1080/10408390802248833.

Miranda JS, Costa BV, de Oliveira IV, de Lima DCN, Martins EMF, de Castro Leite Júnior BR, Almeida do Nascimento Benevenuto WC, Campelo de Queiroz I, Ribeiro da Silva R, Martins ML. Probiotic jelly candies enriched with native Atlantic Forest fruits and Bacillus coagulans GBI-30 6086. LWT– F. Sc & Tech 2020;126:109275. https://doi.org/https://doi.org/10.1016/j.lwt.2020.109275.

Yenrina R, Novelina, Putra DP. The Effect of Citric Acid Addition on Physicochemical and Organoleptic Characteristics of Young Coconut Meat (Cocos Nucifera, L.) and Butterfly Pea (Clitoria ternatea) Sheet Jam. IOP Conf Ser Earth Environ Sci. 2023;1177:12033. https://doi.org/10.1088/1755-1315/1177/1/012033.

Olsson E, Hedenqvist MS, Johansson C, Järnström L. Influence of citric acid and curing on moisture sorption, diffusion and permeability of starch films. Carbohydr Polym. 2013;94:765–772. https://doi.org/https://doi.org/10.1016/j.carbpol.2013.02.006.

Laroche C, Fine F, Gervais P. Water activity affects heat resistance of microorganisms in food powders. Int J Food Microbiol. 2005;97:307–315. https://doi.org/https://doi.org/10.1016/j.ijfoodmicro.2004.04.023.

Tamby Chik C, Bachok S, Baba N, Abdullah A, Abdullah N. Quality characteristics and acceptability of three types of pitaya fruits in a consumer acceptance test. J Tour Hosp Cul Arts. 2011;3:89–98.

Taharuddin NH, Jumaidin R, Mansor MR, Hazrati KZ, Tarique J, Asyraf MRM, Razman MR. Unlocking the Potential of Lignocellulosic Biomass Dragon Fruit (Hylocereus polyrhizus) in Bioplastics, Biocomposites and Various Commercial Applications. Polymers. 2023;15(12):2654. https://doi.org/10.3390/polym15122654.

Tua SM, Apul S, Restuana SD, Rosa T, Maruba P, Posman S, Delima P, Sisilia Y, Oktavia TD. Effect of citric acid and sucrose concentration on the quality of passion fruit jelly with dutch eggplant. IOP Conf Ser Earth Environ Sci. 2018; 205: 012050.

Guneser O. Kinetic Modelling of Betalain Stability and Color Changes in Yogurt During Storage. Pol J Food Nutr Sci. 2021;71:135–145. https://doi.org/10.31883/pjfns/134393.

Lim TW, Lim RLH, Pui LP, Tan CP, Ho CW. Synergistic enhancing effect of xanthan gum, carboxymethyl cellulose and citric acid on the stability of betacyanins in fermented red dragon fruit (Hylocereus polyrhizus) drink during storage. Heliyon. 2023;9:e21025. https://doi.org/https://doi.org/10.1016/j.heliyon.2023.e21025.

Khoo HE, He X, Tang Y, Li Z, Li C, Zeng Y, Tang J, Sun J. Betacyanins and anthocyanins in pulp and peel of red pitaya (Hylocereus polyrhizus cv. Jindu), inhibition of oxidative stress, lipid reducing, and cytotoxic effects. Front Nutr. 2022;9:894438.

Zeece M. Introduction to the Chemistry of Food (1st ed.). Cambridge:Academic Press; 2020. 430 p.

Cejudo-Bastante MJ, Hurtado N, Delgado A, Heredia FJ. Impact of pH and temperature on the colour and betalain content of Colombian yellow pitaya peel (Selenicereus megalanthus). J Food Sci Technol. 2016;53:2405–2013. https://doi.org/10.1007/s13197-016-2215-y.

Goudie KJ, McCreath SJ, Parkinson JA, Davidson CM, Liggat JJ. Investigation of the influence of pH on the properties and morphology of gelatin hydrogels. J Polymer Sci. 2023;61:2316–2332. https://doi.org/https://doi.org/10.1002/pol.20230141.

Wang R, Hartel RW. Citric acid and heating on gelatin hydrolysis and gelation in confectionery gels. Food Hydrocoll. 2022;129:107642. https://doi.org/https://doi.org/10.1016/j.foodhyd.2022.107642.

Zandi M, Hamid M, Mayer C. Effects of concentration, temperature, and pH on chain mobility of gelatin during the early stages of gelation. Iran Polymer J. 2007; 16: 861-870

Vaillant F, Pérez A, Davila I, Dornier M, Reynes R. Colorant and antioxidant properties of red-purple pitahaya (Hylocereus sp.). Fruits. 2005;60:3–12. https://doi.org/10.1051/fruits:2005007.

Harivaindaran KV, Rebecca O, Chandran S. Study of optimal temperature, pH and stability of dragon fruit (Hylocereus polyrhizus) peel for use as potential natural colorant. Pak J Biol Sci. 2008;11 18:2259–2263.

Woo KK, Ngou FH, Ngo LS, Soong WK, Tang PY. Stability of betalain pigment from red dragon fruit (Hylocereus polyrhizus). Am J Food Technol. 2011;6:140–148.

Calva-Estrada SJ, Jiménez-Fernández M, Lugo-Cervantes E. Betalains and their applications in food: The current state of processing, stability and future opportunities in the industry. Food chem: mol sci. 2022; 4:100089.

Maran JP, Manikandan S, Mekala V. Modeling and optimization of betalain extraction from Opuntia ficus-indica using Box–Behnken design with desirability function. Ind Crops Prod. 2013; 49: 304-311.

Pasch JH, von Elbe JH. Betanine Stability in Buffered Solutions Containing Organic Acids, Metal Cations, Antioxidants, or Sequestrants. J Food Sci. 1979;44:72–75. https://doi.org/https://doi.org/10.1111/j.1365-2621.1979.tb10007.x.

Lambros M, Tran T, Fei Q, Nicolaou M. Citric Acid: A Multifunctional Pharmaceutical Excipient. Pharmaceutics. 2022;14:972. https://doi.org/10.3390/pharmaceutics14050972.

Castro-Enríquez DD, Montaño-Leyva B, Del Toro-Sánchez CL, Juaréz-Onofre JE, Carvajal-Millan E, Burruel-Ibarra SE, Tapia-Hernández JA, Barreras-Urbina CG, Rodríguez-Félix F. Stabilization of betalains by encapsulation—a review. J Food Sci Technol. 2020;57:1587–600. https://doi.org/10.1007/s13197-019-04120-x.

Rohin MAK, Abu Bakar A, Ali AM. Total antioxidant activity, total phenolic content and radical scavenging activity both flesh and peel of red pitaya, white pitaya and papaya. Int J Pharm Pharm Sci. 2012;Vol 4,:113–122.

Ruzlan N, Kamarudin KR, Idid SZ, Rehan AM, Koya MS. Antioxidant study of pulps and peels of dragon fruits: a comparative study. Int Food Res J. 2010;17:367-375.

Lourith N, Kanlayavattanakul M. Antioxidant and stability of dragon fruit peel colour. Agro Food Ind Hi Tech. 2013;24:56–58.

Tang W, Li W, Yang Y, Lin X, Wang L, Li C, Yang R. Phenolic compounds profile and antioxidant capacity of pitahaya fruit peel from two red-skinned species (Hylocereus polyrhizus and Hylocereus undatus). Foods. 2021;10:1183.

Hong Quan T, Tieu Yen T, Pham Ngoc Tram G, Ngoc Han T, Phung Tien N, Kaewthong P, Karnjanapratum S. Effect of Red Dragon Fruit (Hylocereus polyrhizus) Peel Powder on Physical, Antioxidative, and Sensory Properties of Biscuits. J Culin Sci Technol. 2024;0:1–15. https://doi.org/10.1080/15428052.2024.2361716.

Wahyuningtias D. The Application of Dragon Fruit Peels as a Dye in Red Velvet Cake. Binus Bus Rev. 2015;6(3):375–382.

Friedman M, Jürgens HS. Effect of pH on the Stability of Plant Phenolic Compounds. J Agric Food Chem. 2000;48:2101–2110. https://doi.org/10.1021/jf990489j.

Rodriguez-Amaya DB. Update on natural food pigments—A mini-review on carotenoids, anthocyanins, and betalains. Food Res. Int. 2019;124:200–205. doi:10.1016/j.foodres.

Enaru B, Drețcanu G, Pop TD, Stǎnilǎ A, Diaconeasa Z. Anthocyanins: Factors Affecting Their Stability and Degradation. Antioxidants. 2021;10(12):1967. https://doi.org/10.3390/antiox10121967

Javan AJ, Nikmanesh A, Keykhosravy K, Maftoon S, Aminzare M, Bayani M, Parsaiemehr M, Raeisi M. Effect of Citric Acid Dipping Treatment on Bioactive Components and Antioxidant Properties of Sliced Button Mushroom (Agaricus bisporus). J Food Qual Hazards Control. 2015;2:20-25.

Preciado-Rangel P, Gaucín-Delgado J, Salas-Pérez L, Sánchez-Chávez E, Mendoza-Vllarreal R, Ortiz J. The effect of citric acid on the phenolic compounds, flavonoids and antioxidant capacity of wheat sprouts. Rev Fac Cienc Agrar. 2018;50:119–127.

Nguyen T, Nandasiri R, Fadairo O, Eskin MNA. The effect of pH on the phenolic content and antioxidant properties of three different mustard extracts. J Food Sci. 2023;88:2882–901. https://doi.org/https://doi.org/10.1111/1750-3841.16655.

Pasquet PL, Julien-David D, Zhao M, Villain-Gambier M, Trébouet D. Stability and preservation of phenolic compounds and related antioxidant capacity from agro-food matrix: Effect of pH and atmosphere. Food Biosci. 2024;57:103586. https://doi.org/https://doi.org/10.1016/j.fbio.2024.103586.

Long X, Li R, Gu J, Zhang L, Guo S, Fan Y, Fan Y, Zhu P. Changes in phenolic compounds of Phyllanthus emblica juice during different storage temperature and pH conditions. J Food Sci. 2024;89(7):4312-4330.

Singh SK, Kaldate R, Bisht A. Citric acid, antioxidant effects in health. In: Nabavi SM, Silva AS (Eds.). Antioxidants Effects in Health: The Bright and Dark Side. Amsterdam: Elsevier; 2022. 309–322 p. https://doi.org/https://doi.org/10.1016/B978-0-12-819096-8.00045-8.

Abdl Aziz F, Temraz A, Hassan M. Metabolites profiling by LC-ESI-MS/MS technique and in-vitro antioxidant activity of Bauhinia madagascariensis Desv. and Bauhinia purpurea L. aerial parts cultivated in Egypt: a comparative study. Al-Azhar Int J Pharm Med Sci. 2024;4:169–188. https://doi.org/10.21608/aijpms.2023.212409.1215.

Ducamp-Collin MN, Ramarson H, Lebrun M, Self G, Reynes M. Effect of citric acid and chitosan on maintaining red colouration of litchi fruit pericarp. Postharvest Biol Technol. 2008;49:241–246. https://doi.org/https://doi.org/10.1016/j.postharvbio.2008.01.009.

Ge H, Wu Y, Woshnak LL, Mitmesser SH. Effects of hydrocolloids, acids and nutrients on gelatin network in gummies. Food Hydrocoll. 2021;113:106549. https://doi.org/https://doi.org/10.1016/j.foodhyd.2020.106549.

Lesmayati S, Qomariah R, Awanis, Anggreany S. Effect of Gelatin and Citric Acid Concentration on Chemical and Organoleptic Properties of Jelly Citrus. IOP Conf Ser Earth Environ Sci. 2022;1024:12025. https://doi.org/10.1088/1755-1315/1024/1/012025.

Pratiwi LJ, Swasti YR, Pranata FS. The quality of red guava (Psidium guajava L.) gummy candies with variation additions of pineapple peel extract paste (Ananas comoscus L. Merr) as a gelling agent. Food Res. 2023;7:63–70.