Optimization of Nanostructured Lipid Carriers for Essential Oil of Artemisia herba-alba
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Abstract
Artemisia herba-alba, commonly known as white wormwood, is an aromatic plant from the Asteraceae family, widely used in traditional medicine due to its pharmacological activities. Essential oils are unstable and susceptible to changes caused by external factors, which can diminish their efficacy. Encapsulation of essential oils can provide controlled drug release, enhance bioavailability, improve physical stability, and increase efficiency while protecting against degradation and reducing evaporation and toxicity. This ensures an optimal pharmacokinetic profile. This research aims to optimize the formulation of nanostructured lipid carriers (NLC) loaded with Artemisia herba-alba essential oil and to evaluate their antioxidant activity. The methodology involves optimizing the NLC formulation using Design Expert (Version 13.0.15, Stat-Ease Inc., Minneapolis, U.S.A.) with response surface methodology/Box-Behnken Design, followed by an evaluation of the optimal formula and antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The optimization results indicated a desirability value of 0.632, with a solid to liquid lipid ratio of 71.36:28.64, a surfactant concentration of 1%, and a sonication time of 6.64 minutes. Both the essential oil of Artemisia herba-alba and the essential oil-loaded nanostructured lipid carriers demonstrated antioxidant activity.
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Awwad A, Poucheret P, Idres AY, Bidel L, Tousch D. The Bitter Asteraceae: An Interesting Approach to Delay the Metabolic Syndrome Progression. NFS J. 2020;18:29–38.
Mohammed MJ, Anand U, Altemimi AB, Tripathi V, Guo Y, Pratap-Singh A. Phenolic Composition, Antioxidant Capacity and Antibacterial Activity of White Wormwood (Artemisia herba-alba). Plants. 2021;10(1):164.
Ouahdani K El, Es-Safi I, Mechchate H, Al-Zahrani M, Qurtam AA, Aleissa M, Bari A, Bousta D. Thymus algeriensis and Artemisia herba-alba Essential Oils: Chemical Analysis, Antioxidant Potential and In Vivo Anti-Inflammatory, Analgesic Activities, and Acute Toxicity. Molecules. 2021;26(22):2780.
Diass K, Oualdi I, Dalli M, Azizi SE, Mohamed M, Gseyra N, Touzani R, Hammouti B. Artemisia herba alba Essential Oil: GC/MS Analysis, Antioxidant Activities with Molecular Docking on S Protein of SARS-CoV-2. Indones J Sci Technol. 2023;8(1):1–18.
Bekka-hadji F, Bombarda I, Djoudi F, Bakour S, Touati A. Chemical Composition and Synergistic Potential of Oils and Antibiotic against Multi-Drug Resistant Bacteria. Molecules. 2022;27:1095.
Cimino C, Maurel OM, Musumeci T, Bonaccorso A, Drago F, Souto EMB, Pignatello R, Carbone C. Essential oils: Pharmaceutical Applications and Encapsulation Strategies Into Lipid-Based Delivery Systems. Pharmaceutics. 2021;13(3):327.
Chura SSD, Memória KAS, Lopes AT, Pelissari FM, Silveira JVW Da, Bezerra J de A, Chaves FCM, Rodrigues AP, Faria JAQA, Carneiro G. Red Sacaca Essential Oil-Loaded Nanostructured Lipid Carriers Optimized by Factorial Design: Cytotoxicity and Cellular Reactive Oxygen Species Levels. Front Pharmacol. 2023;14:1176629.
Singh IR, Pulikkal AK. Preparation, Stability and Biological Activity of Essential Oil-Based Nano Emulsions: A Comprehensive Review. OpenNano. 2022;8:100066.
Nair A, Mallya R, Suvarna V, Khan TA, Momin M, Omri A. Nanoparticles—Attractive Carriers of Antimicrobial Essential Oils. Antibiotics. 2022;11(1):108.
De Sousa DP, Damasceno ROS, Amorati R, Elshabrawy HA, de Castro RD, Bezerra DP, Nunes VRV, Gomes RC, Lima TC. Essential Oils: Chemistry and Pharmacological Activities. Biomolecules. 2023;13(7):1144.
Bonaccorso A, Cimino C, Manno DE, Tomasello B, Serra A, Musumeci T, Puglisi G, Pignatello R, Carbone C. Essential Oil-Loaded NLC for Potential Intranasal Administration. Pharmaceutics. 2021;13(8):1166.
Vieira R, Severino P, Nalone LA, Souto SB, Silva AM, Lucarini M, Durazzo A, Santini A, Souto EB. Sucupira Oil-Loaded Nanostructured Lipid Carriers (NLC): Lipid Screening, Factorial Design, Release Profile, and Cytotoxicity. Molecules. 2020;25:685.
Najjari N, Sari S, Saffari M, Kelidari H, Nokhodchi A. Formulation Optimization and Characterization of Pistacia atlantica Desf. Essential Oil-Loaded Nanostructured Lipid Carriers On The Proliferation of Human Breast Cancer Cell Line SKBR3 (In Vitro Studies). J Herb Med. 2022;36:100600.
Da’i M, Wikantyasning E, Azis FK, Sholikhah M, Suprapto S, Suhendi A, Kusumowati ITK. Cytotoxic Activity of Zerumbone-Loaded Chitosan Oleic Acid Nanoparticles. J Int Pharm Res. 2019;46(4):447–550.
Wikantyasning E, Andasari S, Da’i M, Nabila A. Nanoencapsulation of Zerumbone in Oleic Acid-Modified Chitosan Nanoparticles. In: ICCBB. 2017. p. 105–109.
Da’i M, Wikantyasning E, Saifudin A, Setiyadi G, Wahyuni A, Melia A, Suhendi A. Synthesis of Nanoencapsulation of Alpinia Galanga L. Extract Using Chitosan-Alginate and Cytotoxic Activity on MCF-7 Cell Lines. Int J Pharm Res. 2019;11(1):11540–11544.
Miranda M, Cruz MT, Vitorino C, Cabral C. Nanostructuring Lipid Carriers using Ridolfia segetum (L.) Moris Essential Oil. Mater Sci Eng C. 2019;103:109804.
Yari E, Sari S, Kelidari H, Asare-Addo K, Nokhodchi A. Effect of Rosa damascena Essential Oil Loaded in Nanostructured Lipid Carriers on the Proliferation of Human Breast Cancer Cell Line MDA-MB-231 in Comparison with Cisplatin. J Pharm Innov. 2024;19(4):1–13.
Carneiro SB, Kreutz T, Limberger RP, Teixeira HF, da Veiga Júnior VF, Koester LS. Piper aduncum Essential Oil Rich in Dillapiole: Development of Hydrogel-Thickened Nanoemulsion and Nanostructured Lipid Carrier Intended for Skin Delivery. Pharmaceutics. 2022;14(11):2525.
Gomaa E, Fathi HA, Eissa NG, Elsabahy M. Methods for Preparation of Nanostructured Lipid Carriers. Methods. 2022;199:3–8.
Khezri K, Farahpour M, Rad SM. Efficacy of Mentha Pulegium Essential Oil Encapsulated Into Nanostructured Lipid Carriers As An In Vitro Antibacterial and Infected Wound Healing Agent. Colloids Surfaces A Physicochem Eng Asp. 2020;589:124414.
Ling Q, Zhang B, Wang Y, Xiao Z, Hou J, Xiao C, Liu Y, Jin Z. Chemical Composition and Antioxidant Activity of the Essential Oils of Citral-Rich Chemotype Cinnamomum camphora and Cinnamomum bodinieri. Molecules. 2022;27(21):7356.
Okolie NP, Falodun A, Davids O. Evaluation of the Antioxidant Activity of Root Extract of Pepper Fruit (Dennetia Tripetala), and It’S Potential for the Inhibition of Lipid Peroxidation. African J Tradit Complement Altern Med. 2014;11(3):221–227.
Egharevba E, Chukwuemeke-Nwani P, Eboh U, Okoye E, Bolanle IO, Oseghale IO, Imieje VO, Erharuyi O, Falodun A. Evaluation of the Antioxidant and Hypoglycaemic Potentials of the Leaf Extracts of Stachytarphyta jamaicensis (Verbenaceae). Trop J Nat Prod Res. 2019;3(5):170–174.
Agrawal M, Saraf S, Pradhan M, Patel RJ, Singhvi G, Ajazuddin, Alexander A. Design and Optimization of Curcumin Loaded Nano Lipid Carrier System Using Box-Behnken Design. Biomed Pharmacother. 2021;141:111919.
Solikah A, Iskandarsyah, Harmita. Box-Behnken Design for Optimization of Nanostructured Lipid Carrier Sonication Conditions from Mixtures of Palm Stearin and Palm Olein. Indones J Pharm. 2021;32(4):522–528.
Gordillo-Galeano A, Mora-Huertas CE. Solid Lipid Nanoparticles and Nanostructured Lipid Carriers: A Review Emphasizing on Particle Structure and Drug Release. Eur J Pharm Biopharm. 2018;133:285–308.
Akbari J, Saeedi M, Ahmadi F, Hashemi SMH, Babaei A, Yaddollahi S, Rostamkalaei SS, Asare-Addo K, Nokhodchi A. Solid Lipid Nanoparticles and Nanostructured Lipid Carriers: A Review of The Methods of Manufacture And Routes of Administration. Pharm Dev Technol. 2022;27(5):525–544.
Salvi VR, Pawar P. Nanostructured Lipid Carriers (NLC) System: A Novel Drug Targeting Carrier. J Drug Deliv Sci Technol. 2019;51:255–267.
Rabima R, Sari MP. Entrapment Efficiency and Drug Loading of Curcumin Nanostructured Lipid Carrier (NLC) Formula. Pharmaciana. 2019;9(2):299–306.
Subramaniam B, Siddik ZH, Nagoor NH. Optimization of Nanostructured Lipid Carriers: Understanding The Types, Designs, and Parameters in The Process of Formulations. J Nanoparticle Res. 2020;22(6):141.
Sopyan I, Gozali D, Sriwidodo, Guntina RK. Design-Expert Software (DOE): an Application Tool for Optimization in Pharmaceutical Preparations Formulation. Int J Appl Pharm. 2022;14(4):55–63.