Evaluation of Antirheumatoid Arthritis Activity of Aqueous Methanol Extract of Globba winitti Rhizome
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Abstract
Rheumatoid arthritis is an autoimmune disorder affecting about 0.35 – 1% of the world population. This study aimed to assess the effectiveness of the aqueous methanol extract of Globba winitti (AMEGW) in the treatment of Freund's Complete Adjuvant (FCA)-induced arthritis in rats. AMEGW was obtained by maceration in methanol, the acute toxicity of the extract was evaluated by single oral administration of 2000 mg/kg dose of AMEGW to Swiss mice. Arthritis was induced by intraplantar injection of FCA (0.1 mL) to the rats. The FCA-induced arthritic rats were treated with AMEGW at doses of 100, 200, and 400 mg/kg per oral,once daily for 28 days. The anti-arthritic effect of the extract was evaluated by measuring various parameters, including paw volume, joint diameter, pain threshold, thermal hyperalgesia (paw withdrawal latency), mechanical nociceptive threshold, and body weight. On day 28, rats were sacrificed, blood samples were collected for haematological, biochemical, and antioxidant analysis. Radiological and histopathological examination of ankle joints were also performed. AMEGW demonstrated anti-arthritic activity by reducing paw volume and joint diameter, accompanied by an increase in pain threshold, mechanical nociceptive threshold, paw withdrawal latency, and body weights of arthritic rats in a dose-dependent manner. AMEGW at doses of 400 and 200 mg/kg reversed the alterations in haematological, biochemical, and antioxidant parameters caused by FCA-induced arthritis. Furthermore, AMEGW effectively suppressed joint destruction, as evidenced by radiological and histopathological analysis. Therefore, G. winitti exhibits potential as a viable preventive or therapeutic alternative for the treatment of inflammatory conditions such as rheumatoid arthritis.
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Radu A-F and Bungau SG. Management of Rheumatoid Arthritis An Overview. Cells. 2021; 10(11):2857-2858.
Combe B, Landewe R, Daien CI, Hua C, Aletaha D, Álvaro-Gracia JM . Update of the EULAR recommendations for managing early arthritis. Ann Rheum Dis. 2017; 76(6):948–959.
Mazumder S, De R, Sarkar S., Siddiqui AA, Saha SJ, Banerjee C, Iqbal MS, Nag S, Debsharma S, Bandyopadhyay U. Selective scavenging of intra-mitochondrial superoxide corrects diclofenac-induced mitochondrial dysfunction and gastric injury-A novel gastroprotective mechanism independent of gastric acid suppression. Biochem. Pharmacol. 2016; 121:33–51.
Abhishek A, Doherty M, Kuo CF, Mallen CD, Zhang W, Grainge MJ. Rheumatoid arthritis is getting less frequent-results of a nationwide population-based cohort study. Rheumatol. 2017;5 6(5):736-744.
TC Messemaker, Huizinga TW, Kurreeman F. Immunogenetics of rheumatoid arthritis understanding functional implications. J Autoimmun. 2015; 64:74-81.
Chaurasia N , Singh A, Singh IL, Singh T, Tiwari T. Cognitive dysfunction in patients of rheumatoid arthritis. J Fam Med Prim Care. 2020; 9:2219–2225.
Guo Q, Wang Y, Xu D, Nossent J, Pavlos NJ, Xu J. Rheumatoid arthritis- Pathological mechanisms and modern pharmacologic therapies. Bone Res. 2018; 6:1–15.
Del Grossi Moura M, Cruz Lopes L, Silva MT, Barberato-Filho S, Motta RHL, Bergamaschi CC. Use of steroid and nonsteroidal anti-inflammatories in the treatment of rheumatoid arthritis: Systematic review protocol. Medicine (Baltimore). 2018; 97(41):e12658.
Ong SC, Yeong SW, Azman N, Tan BY, Shafie AA, Ooi GS, Harun SN. Factors predicting traditional andcomplementary medicine use among the general public in Malaysia. Trop J Nat Prod Res. 2022; 6(7):1165-1173 .
Falodun A,Siraj R,Chowdary MI. GC-MS Insecticidal leaf essential oil of P.staudtii.Trop J.Pharm Res. 2009; 82:139-143.
Nhung TT and Quoc LP. Investigation of the Inflammatory, Antipyretic, and Analgesic Potential of Ethanol Extract from Hedyotis capitellata Leaves in Mice .Trop J Nat Prod Res . 2023; 7(12):5501–5508.
Pal SK and Lawal IU. Current Status and Future of Traditional, Complementary and Integrative Medicine in Nigeria. Trop J Nat Prod Res. 2023; 7(12):5403-5409.
Ouryemchi I, Oubihi A, Taibi M, Elbouzidi A, Jaber H, Haida S, Tarfaoui K, Atfaoui K, Chaabane K, El Guerrouj B, Bellaouchi R, Asehraou A, Addi M, Benzakour A, Ouhssine M. GC-MS Characterization, Antioxidant, Antimicrobial and Insecticidal Potential of Moroccan Cuminum Cyminum Essential Oil .Trop J Nat Prod Res. 2024; 8(2):6108-6114.
Mehta A, Sethiya N, Mehta C, Shah G. Anti-arthritis activity of roots of Hemidesmus indicus in rats. Asian Pac J Trop Med. 2012; 6 (5):130–135.
Mahayasa NW, Mahayasih PGMW, Kaldicson A, Achmad AS, Rahayu ST. Effect of Extraction Methods on the Antioxidants and Alpha-Glucosidase Inhibitory Activity of Borassus flabellifer Fruit Fiber. Trop J Nat Prod Res. 2024; 8(5):7063-7067.
Gao Y, Wang T, Wang G, Li G, Sun C, Jiang Z,Yang J. Preclinical safety of ginsenoside compound K - Acute and 26 week oral toxicity studies in mice and rats. Food Chem. Toxicol. 2019; 131:110578 .
Karwasra R, Sharma S, Sharma N, Khanna K. Protective effect of Boerhavia diffusa in attenuating pro-inflammatory cytokines and inhibition of activated NF-κB-TNF-α-Nrf2 in Freund’s adjuvant-induced rheumatoid arthritis. Indian J Pharm Educ Res. 2021; 55(2s):s563–s571.
Aloke C, Ibiam U, Orji O, Ugwuja E, Ezeani N, Aja P, Obasi NA. Antiarthritic potential of ethanol and aqueous extracts of stem bark of Cleistopholis patens on complete Freund’s adjuvant-induced rheumatoid arthritis in rats. J.Ayurv Integr Med. 2021; 12(1):28–34.
Banchet G, Boettger M, Fischer N, Gajda M, Brauer R, Schaible H. Experimental arthritis causes tumor necrosis factor-a-dependent infiltration of macrophages into rat dorsal root ganglia which correlates with pain-related behavior. Brain Behav.Immun. 2022; 106:289-306.
Authier N, Gillet J, Fialip J, Eschalier A, Coudore F. A New Animal Model of Vincristine-Induced Nociceptive Peripheral Neuropathy. Neurotoxicol. 2003; 24:797–805.
Ramteke V, Tandan S, Kumar D, Devi A, Shukla M, Vellanki R. Increased hyper- algesia by 5-nitro-2, 3- (phenylpropylamino)-benzoic acid (NPPB), a chloride channel blocker in crush injury-induced neuropathic pain in rats. Pharmacol Biochem Behav. 2009; 91:417–422.
Jalalpure S, Mandavkar Y, Khalure P, Shinde G, Shelar P, Shah A. Antiarthritic activity of various extracts of Mesua ferrea seed. J Ethnopharmacol. 2011; 138:700–704.
Pepys M and Hirschfield G. C-reactive protein- a critical update. J Clin Invest. 2003; 11:1805–1812.
Asquith D, Miller A, McInnes I, Liew F. Animal models of rheumatoid arthritis. Eur J Immunol . . 009; 39(8):2040–2044.
Mythilypriya R, Shanthi P, Sachdanandam P. Salubrious effect of Kalpaamruthaa, a modified indigenous preparation in adjuvant-induced arthritis in rats – A biochemical approach. Chem Biol Interact. 2008; 173:148–158.
Patil M, Kandhare A, Bhise S. Antiarthritic and anti-inflammatory activity of Xanthium srtumarium ethanolic extract in Freund’s complete adjuvant induced arthritis. Biomed Aging Pathol. 2012; 2:6–15.
Hu F, Hepburn R, Li Y, Chen M, Radloff E, Daya S. Effect of ethanol and water extracts of Propolis on acute inflammatory animal models. J Ethnopharmacol. 2005; 100:276-283.
Misera HP and Fridocich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for SOD. J Biol Chem. 1972; 31:70–75.
Morgon M, Depirre J, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S- transferase activities in rat lung and liver. Biochem Biophys Acta 1979; 582:67–78.
Slater T and Sawyer B. The stimulatory effects of carbon tetrachloride and other halogenoalkanes or peroxidative reactions in rat liver fraction in vitro. Biochem J. 1971; 123:805–814.
Verpoorte R. Exploration of nature’s chemodiversity- the role of secondary metabolites as leads in drug development. Drug Discov Today. 1999;3:232–238.
Amresh G, Singh P, Rao C. Antinociceptive and antiarthritic activity of Cissam- pelos pareira roots. J Ethnopharmacol. 2017; 111:531–536.
Patil K, and Suryavanshi J. Effect of Celastrus paniculatus seed on adjuvant induced arthritis in rats. Pharmacogn Mag. 2007; 3:177–181.
Vijayakumar S, Dhanapal R, Sarathchandran I, Saravana A, Vijaya J. Evaluation of antioxidant activity of Ammania baccifera whole plant extract in rats. Asian Pac J Trop Biomed. 2012; 2(1):116–119.
Noguchi M, Kimoto A, Kobayashi S, Yoshino T, Miyata K, Sasamata M. Effect of celecoxib, a cyclooxygenase-2 inhibitor, on the pathophysiology of adjuvant arthritis in rat. Eur J Pharmaco l . 2005; 513:229–235.
Arulmozhi S, Mazumder P, Sathiyanarayanan L, Ashok P. Antiarthritic and antioxidant activity of leaves of Alstonia scholaris Linn R. Br. Eur J Integr Med. 2011; 3:83–90.
Szygula Z, Lubkowska A, Giemza C, Skrzek A, Bryczkowska I, Dołęgowska B. Hematological parameters, and hematopoietic growth factors EPO and IL-3 in response to whole-body cryostimulation (WBC) in military academy students. PloS One. 2014; 9(4):e93096.
Tiwari V, Jandu JS, Bergman MJ. Rheumatoid Factor. [Online]. 2024 [cited Jan- 10]. Available from: https://www.ncbi.nlm.nih.gov/books/NBK532898.
Guo Q, Wang Y, Xu D. Rheumatoid arthritis- pathological mechanisms and modern pharmacologic therapies. Bone Res. 2018; 6:15 .
Chen W, Wang W, Zhou L, Zhou J, He L, Li J, Xu X, Wang J, Wang L. Elevated AST/ALT ratio is associated with all-cause mortality and cancer incident. Clin Lab. 2022;36(5):e24356.
Hemshekhar M, Sunitha K, Thushara R, Sebastin M, Shanmuga M, Kemparaju K. The antiarthritic and anti-inflammatory propensity of 4-methyl esculetin a coumarin derivative. Biochim. 2013; 95:1326–1335.
Kizilntuc A, Cogalgil S, Cerrahoglu L. Carnitine and antioxidants levels in patients with rheumatoid arthritis. Scand J Rheumatol. 1998; 27:441–445.
Hassan M, Hadi R, Al-Rawi Z, Padron V, Stohs S. The glutathione defense system in the pathogenesis of rheumatoid arthritis. J Appl Toxicol. 2001; 21:69–73.
Pedernera A, Guardia T, Calderón C, Rotelli A, De la Rocha N, Genaro S. Anti-ulcerogenic and anti-inflammatory activity of the methanolic extract of Larrea divaricata in the rat. J Ethnopharmacol. 2006; 105:415–420.
Kaushal G and Shao J. Oral Delivery of β-lactamase by Lactococcus Lactis Subsp. Lactis Transformed with Plasmid Ss80. Int J Pharm. 2006; 5(6):210-216.