Gas Chromatography-Mass Spectrometry Profiling, Antioxidant and Antibacterial Evaluations of Essential Oils Extracted from Mentha piperita and Mentha pulegium

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Published: Dec 29, 2024
DOI: https://doi.org/10.26538/tjnpr/v8i12.6
Keywords:
in silico, Activity, Antioxidant, Antibacterial, Chemical composition, Essential oil, Mentha pulegium, Mentha piperita

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Mohamed Amine El maimouni
Materials, Processes, Catalysis, and Environment Laboratory, Higher School of Technology of Fez, Sidi Mohamed Ben Abdellah University, Imouzzer Road, Fez, Morocco 
Soukaina El amrani
Materials, Processes, Catalysis, and Environment Laboratory, Higher School of Technology of Fez, Sidi Mohamed Ben Abdellah University, Imouzzer Road, Fez, Morocco 
Sanae Lairini
Materials, Processes, Catalysis, and Environment Laboratory, Higher School of Technology of Fez, Sidi Mohamed Ben Abdellah University, Imouzzer Road, Fez, Morocco 
Rabia Bouslamti
Materials, Processes, Catalysis, and Environment Laboratory, Higher School of Technology of Fez, Sidi Mohamed Ben Abdellah University, Imouzzer Road, Fez, Morocco
Mohammed Er-rajy
Laboratoire d'Ingénierie Modélisation et Analyses des Systèmes (LIMAS). Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco  
Somdutt Mujwar
Chitkara College of Pharmacy, Chitkara University, Rajpura, Punjab, India  
Mohammed Chalkha
Laboratory of Material Engineering for the Environment and Natural Resources, Faculty of Sciences and Techniques, University of Moulay Ismail of Meknès, Boutalamine, Errachidia, Morocco 
Khalid Chebbac
Laboratory of Biotechnology and Preservation of Natural Resources, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdallah University, Fez, Morocco 
Fouad El Akhal
Higher Institute of Nursing Professions and Health Techniques of Fez, Regional Directorate of Health, El Ghassani Hospital, Dhar El Mehraz, Fez, Morocco 
Abdelhakim El Ouali Lalami
Materials, Processes, Catalysis, and Environment Laboratory, Higher School of Technology of Fez, Sidi Mohamed Ben Abdellah University, Imouzzer Road, Fez, Morocco 

Abstract

Synthetic food preservatives protect against oxidation and pathogens but can harm consumer health. Plant-based bioactive components like phenolic acids, flavonoids, and essential oils (EOs) are crucial for safe alternatives. The study investigated the chemical composition and biological activities of EOs extracted from Mentha piperita and Mentha pulegium. Essential oils from both plant species were extracted using hydro-distillation method. Gas chromatography-mass spectrometry (GC-MS) profiling was performed to determine the EOs' constituents. The EOs were assessed for antioxidant properties using three assays, while their antibacterial activity was evaluated against Escherichia coli and Staphylococcus aureus. Molecular docking and dynamic simulation studies were performed to elucidate the interaction between EO constituents and target enzymes. The results revealed that the main components in M. piperita and M. pulegium EOs were isomintlactone (35.55%) and pulegone (74.04%), respectively. The EOs of M. piperita and M. pulegium exhibited remarkable antioxidant activity, with inhibitory concentration (IC50) values of 3.24±0.22 and 3.51±0.22 mg/mL, respectively, in the 2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH) assay. Strong antibacterial efficacy was observed with a minimum inhibitory concentration (MIC) of 4% for M. pulegium and moderate effects for M. piperita with MIC values of 0.5% against Staphylococcus aureus and its ATCC29213, and 1% against Escherichia coli and its ATCC25922. Molecular docking and dynamic molecular simulations showed that the identified chemical constituents in the test EOs have a good affinity to the target enzymes. These findings suggest that M. piperita and M. pulegium EOs are promising natural sources of antioxidant and antibacterial agents, potentially valuable in food preservation and health applications. 

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How to Cite
El maimouni, M. A., El amrani, S., Lairini, S., Bouslamti, R., Er-rajy, M., Mujwar, S., Chalkha, M., Chebbac, K., El Akhal, F., & Lalami, A. E. O. (2024). Gas Chromatography-Mass Spectrometry Profiling, Antioxidant and Antibacterial Evaluations of Essential Oils Extracted from Mentha piperita and Mentha pulegium. Tropical Journal of Natural Product Research (TJNPR), 8(12), 9386-9398. https://doi.org/10.26538/tjnpr/v8i12.6
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Vol. 8 No. 12 (2024): Tropical Journal of Natural Product Research (TJNPR)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biographies

Mohammed Chalkha, Laboratory of Material Engineering for the Environment and Natural Resources, Faculty of Sciences and Techniques, University of Moulay Ismail of Meknès, Boutalamine, Errachidia, Morocco 

Engineering Laboratory of Organometallic, Molecular Materials and Environment, Faculty of Sciences Dhar EL Mahraz, Sidi Mohamed Ben Abdellah University, Atlas, Fez, Morocco  

Abdelhakim El Ouali Lalami, Materials, Processes, Catalysis, and Environment Laboratory, Higher School of Technology of Fez, Sidi Mohamed Ben Abdellah University, Imouzzer Road, Fez, Morocco 

Higher Institute of Nursing Professions and Health Techniques of Fez, Regional Directorate of Health, El Ghassani Hospital, Dhar El Mehraz, Fez, Morocco

How to Cite

El maimouni, M. A., El amrani, S., Lairini, S., Bouslamti, R., Er-rajy, M., Mujwar, S., Chalkha, M., Chebbac, K., El Akhal, F., & Lalami, A. E. O. (2024). Gas Chromatography-Mass Spectrometry Profiling, Antioxidant and Antibacterial Evaluations of Essential Oils Extracted from Mentha piperita and Mentha pulegium. Tropical Journal of Natural Product Research (TJNPR), 8(12), 9386-9398. https://doi.org/10.26538/tjnpr/v8i12.6

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