Anti-Inflammatory Activity of Qutsh Al Hindi (Saussurea lappa) Root Fractions: In Vitro Assay and Characterization of its Active Compound

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Published: Dec 2, 2024
DOI: https://doi.org/10.26538/tjnpr/v8i11.35
Keywords:
LC-MS, Costunolide, Anti-inflammatory activity, Saussurea lappa

Main Article Content

Sukmawati
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, University of Padjadjaran, Indonesia
Ida Musfiroh
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, University of Padjadjaran, Indonesia
Muchtaridi Muchtaridi
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, University of Padjadjaran, Indonesia
Adryan Fristiohady
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, University Halu Oleo, Kendari, Indonesia
Nur Kusaira Khairul Ikram
Institute of Biological Sciences, Faculty of Science, University Malaya, Kuala Lumpur, 50603, Malaysia

Abstract

Inflammation is a physiological response involved in various pathological conditions. Natural products such as Saussurea lappa (S.lappa) offer potential anti-inflammatory treatments with fewer side effects. This study aimed to evaluate the anti-inflammatory activity of the fractions of S. lappa root, and identify the active compound. S.lappa fractions were obtained by liquid-liquid fractionation of the crude ethanol extract using n-hexane and ethyl acetate. The antiinflammatory activity of the fractions was evaluated in vitro using the erythrocyte membrane stabilization method. The potentially active compound in the ethyl acetate fraction was identified by liquid chromatography-mass spectrometric (LC-MS) analysis. Results showed that the n-hexane and ethyl acetate fractions of S.lappa exhibited anti-inflammatory activity in vitro. The ethyl acetate fraction showed a higher anti-inflammatory activity (IC50= 1.113 ppm), than the n-hexane fraction with an IC50 of 1.206 ppm. LC-MS analysis identified costunolide as the potentially active anti-inflammatory compound in the ethyl acetate fraction of S. lappa root extract. Therefore, S. lappa root has shown promising anti-inflammatory properties that could be harnessed for therapeutic purposes.
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How to Cite
Sukmawati, Musfiroh, I., Muchtaridi, M., Fristiohady, A., & Ikram, N. K. K. (2024). Anti-Inflammatory Activity of Qutsh Al Hindi (Saussurea lappa) Root Fractions: In Vitro Assay and Characterization of its Active Compound. Tropical Journal of Natural Product Research (TJNPR), 8(11), 9219-9223. https://doi.org/10.26538/tjnpr/v8i11.35
Issue
Vol. 8 No. 11 (2024): Tropical Journal of Natural Product Research (TJNPR)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biography

Sukmawati, Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, University of Padjadjaran, Indonesia

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Muslim University, Indonesia

How to Cite

Sukmawati, Musfiroh, I., Muchtaridi, M., Fristiohady, A., & Ikram, N. K. K. (2024). Anti-Inflammatory Activity of Qutsh Al Hindi (Saussurea lappa) Root Fractions: In Vitro Assay and Characterization of its Active Compound. Tropical Journal of Natural Product Research (TJNPR), 8(11), 9219-9223. https://doi.org/10.26538/tjnpr/v8i11.35

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