Chemical Constituents and Antioxidant Activity of Ultrasonication-Assisted Aqueous Extract of Pumpkin (Cucurbita moschata) Seeds

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Published: Dec 2, 2024
DOI: https://doi.org/10.26538/tjnpr/v8i11.34
Keywords:
Ultrasonification-Assited extraction, Cucurbita moschata, Antioxidant, Gas Chromatography-Mass Spectrometry

Main Article Content

Erinda N. Pratiwi
Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Soetrisno
Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Bambang Purwanto
Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Yulia L.R. Dewi
Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Eti P. Pamungkasari
Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Brian Wasita
Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Abstract

Plants are widely used in alternative medicine, and hold significant potential for novel drug discovery and development. A notable example of such plant is the pumpkin plant (Cucurbita moschata) which offers numerous health benefits due to its antioxidants properties, especially the fruits and seeds. Therefore, this study aimed to identify the chemical constituents of pumpkin seeds and evaluate ots antioxidant activity. Pumpkin seeds were extracted by ultrasonication-assisted extraction (UAE) method using water as the extraction solvent. The chemical composition of the extract was identified by gas chromatographic-mass spectrometric (GC-MS) analysis. The extract was evaluated for its antioxidant activity using the 1-1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. GC-MS analysis revealed numerous chemical constituents including; Squalene, Stigmasterol, Hexadecanoic acid methyl ester, 2-Pentylcyclopropyl cyclopropanebutanoic acid, Hexadecanoic acid ethyl ester, 9,12-Octadecadienoic acid (Z, Z)-methyl ester, 16-methyl-Heptadecanoic acid methyl ester, Ethyl 9-cis,11-trans-octadecadienoate, and several forms of Ethyl iso-allocholate and its derivatives. Other compounds identified are 3-(octadecyloxy)propyl ester of oleic acid, 1-Heptatriacotanol, 8,14-Seco-3,19-epoxyandrost derived from Ethyl iso-allocholate, Tocopherol, Tris(acetyloxy) derivative of 3-Pyridinecarboxylic acid, 4,4-Dimethyl cholesta-22,24-dien-5-ol, Triol derivative of 9,10-Secocholesta-5,7,10(19)-triene presented as the 3ß,5Z,7E isomer, and 3-hydroxy Spirost-8-en-11-one with specific stereochemistry of 3β,5α,14β,20β,22β,25R. the aqueous extract of pumpkin seeds exhibited potent antioxidant activity with IC50 value of 8.625 ± 0.169 µg/mL. In conclusion, pumpkin seed aqueous extract obtained through UAE method contains several compounds that may act as antioxidants. 

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How to Cite
Pratiwi, E. N., Soetrisno, Purwanto, B., Dewi, Y. L., Pamungkasari, E. P., & Wasita, B. (2024). Chemical Constituents and Antioxidant Activity of Ultrasonication-Assisted Aqueous Extract of Pumpkin (Cucurbita moschata) Seeds. Tropical Journal of Natural Product Research (TJNPR), 8(11), 9215-9218. https://doi.org/10.26538/tjnpr/v8i11.34
Issue
Vol. 8 No. 11 (2024): Tropical Journal of Natural Product Research (TJNPR)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biographies

Erinda N. Pratiwi, Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Midwifery Program, Faculty of Health, Universitas Kusuma Husada Surakarta, Surakarta, Indonesia

Soetrisno, Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Sebelas Maret-Universitas Sebelas Maret Hospital, Surakarta, Indonesia

Bambang Purwanto, Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Department of Internal Medicine, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Yulia L.R. Dewi, Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Department of Nutrition, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Eti P. Pamungkasari, Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Department of Public Health, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Brian Wasita, Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Department of Anatomical Pathology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

How to Cite

Pratiwi, E. N., Soetrisno, Purwanto, B., Dewi, Y. L., Pamungkasari, E. P., & Wasita, B. (2024). Chemical Constituents and Antioxidant Activity of Ultrasonication-Assisted Aqueous Extract of Pumpkin (Cucurbita moschata) Seeds. Tropical Journal of Natural Product Research (TJNPR), 8(11), 9215-9218. https://doi.org/10.26538/tjnpr/v8i11.34

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